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Protein

Ribonuclease H2 subunit A

Gene

RNH201

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Divalent metal cation1
Metal bindingi40Divalent metal cationBy similarity1
Metal bindingi155Divalent metal cation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: InterPro
  • RNA-DNA hybrid ribonuclease activity Source: SGD

GO - Biological processi

  • DNA replication, removal of RNA primer Source: SGD
  • mismatch repair Source: GO_Central

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMetal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33101-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit A (EC:3.1.26.4)
Short name:
RNase H2 subunit A
Alternative name(s):
RNase H(201)
RNase H(35)
Ribonuclease HI large subunit
Short name:
RNase HI large subunit
Ribonuclease HI subunit A
Gene namesi
Name:RNH201
Synonyms:RNH35
Ordered Locus Names:YNL072W
ORF Names:N2369
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL072W
SGDiS000005016 RNH201

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39D → A: Abolishes enzyme activity. 1 Publication1
Mutagenesisi155D → A: Abolishes enzyme activity. 1 Publication1
Mutagenesisi183D → A: Strongly impairs enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001117181 – 307Ribonuclease H2 subunit AAdd BLAST307

Proteomic databases

MaxQBiP53942
PaxDbiP53942
PRIDEiP53942

Interactioni

Subunit structurei

The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35751, 219 interactors
DIPiDIP-4565N
IntActiP53942, 3 interactors
MINTiP53942
STRINGi4932.YNL072W

Structurei

3D structure databases

ProteinModelPortaliP53942
SMRiP53942
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. Eukaryotic subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010768
HOGENOMiHOG000100290
InParanoidiP53942
KOiK10743
OMAiREECRFF
OrthoDBiEOG092C57KP

Family and domain databases

Gene3Di1.10.10.460, 1 hit
3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR004649 RNase_H2_suA
IPR001352 RNase_HII/HIII
IPR024567 RNase_HII/HIII_dom
IPR023160 RNase_HII_hlx-loop-hlx_cap_dom
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10954 PTHR10954, 1 hit
PfamiView protein in Pfam
PF01351 RNase_HII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00729 TIGR00729, 1 hit

Sequencei

Sequence statusi: Complete.

P53942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM
60 70 80 90 100
VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI
110 120 130 140 150
GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS
160 170 180 190 200
HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR
210 220 230 240 250
DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT
260 270 280 290 300
CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR

TLDNWYR
Length:307
Mass (Da):34,875
Last modified:October 1, 1996 - v1
Checksum:i60B1005F674ECC88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86470 Genomic DNA Translation: CAA60188.1
Z71348 Genomic DNA Translation: CAA95946.1
BK006947 Genomic DNA Translation: DAA10473.1
PIRiS53908
RefSeqiNP_014327.1, NM_001182910.1

Genome annotation databases

EnsemblFungiiYNL072W; YNL072W; YNL072W
GeneIDi855652
KEGGisce:YNL072W

Similar proteinsi

Entry informationi

Entry nameiRNH2A_YEAST
AccessioniPrimary (citable) accession number: P53942
Secondary accession number(s): D6W1A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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