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P53942

- RNH2A_YEAST

UniProt

P53942 - RNH2A_YEAST

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Protein

Ribonuclease H2 subunit A

Gene

RNH201

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Divalent metal cation
Metal bindingi40 – 401Divalent metal cationBy similarity
Metal bindingi155 – 1551Divalent metal cation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA-DNA hybrid ribonuclease activity Source: SGD

GO - Biological processi

  1. DNA replication, removal of RNA primer Source: SGD
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33101-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit A (EC:3.1.26.4)
Short name:
RNase H2 subunit A
Alternative name(s):
RNase H(201)
RNase H(35)
Ribonuclease HI large subunit
Short name:
RNase HI large subunit
Ribonuclease HI subunit A
Gene namesi
Name:RNH201
Synonyms:RNH35
Ordered Locus Names:YNL072W
ORF Names:N2369
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL072w.
SGDiS000005016. RNH201.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: SGD
  3. ribonuclease H2 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391D → A: Abolishes enzyme activity. 1 Publication
Mutagenesisi155 – 1551D → A: Abolishes enzyme activity. 1 Publication
Mutagenesisi183 – 1831D → A: Strongly impairs enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Ribonuclease H2 subunit APRO_0000111718Add
BLAST

Proteomic databases

MaxQBiP53942.
PaxDbiP53942.
PeptideAtlasiP53942.

Expressioni

Gene expression databases

GenevestigatoriP53942.

Interactioni

Subunit structurei

The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

Binary interactionsi

WithEntry#Exp.IntActNotes
RNH202Q056353EBI-15663,EBI-33940
RNH203Q123382EBI-15663,EBI-33805

Protein-protein interaction databases

BioGridi35751. 67 interactions.
DIPiDIP-4565N.
IntActiP53942. 2 interactions.
MINTiMINT-532801.
STRINGi4932.YNL072W.

Structurei

3D structure databases

ProteinModelPortaliP53942.
SMRiP53942. Positions 20-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. Eukaryotic subfamily.Curated

Phylogenomic databases

eggNOGiCOG0164.
GeneTreeiENSGT00390000010768.
HOGENOMiHOG000100290.
InParanoidiP53942.
KOiK10743.
OMAiGPPEKYQ.
OrthoDBiEOG76X68V.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.

Sequencei

Sequence statusi: Complete.

P53942-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM
60 70 80 90 100
VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI
110 120 130 140 150
GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS
160 170 180 190 200
HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR
210 220 230 240 250
DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT
260 270 280 290 300
CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR

TLDNWYR
Length:307
Mass (Da):34,875
Last modified:October 1, 1996 - v1
Checksum:i60B1005F674ECC88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86470 Genomic DNA. Translation: CAA60188.1.
Z71348 Genomic DNA. Translation: CAA95946.1.
BK006947 Genomic DNA. Translation: DAA10473.1.
PIRiS53908.
RefSeqiNP_014327.1. NM_001182910.1.

Genome annotation databases

EnsemblFungiiYNL072W; YNL072W; YNL072W.
GeneIDi855652.
KEGGisce:YNL072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86470 Genomic DNA. Translation: CAA60188.1 .
Z71348 Genomic DNA. Translation: CAA95946.1 .
BK006947 Genomic DNA. Translation: DAA10473.1 .
PIRi S53908.
RefSeqi NP_014327.1. NM_001182910.1.

3D structure databases

ProteinModelPortali P53942.
SMRi P53942. Positions 20-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35751. 67 interactions.
DIPi DIP-4565N.
IntActi P53942. 2 interactions.
MINTi MINT-532801.
STRINGi 4932.YNL072W.

Proteomic databases

MaxQBi P53942.
PaxDbi P53942.
PeptideAtlasi P53942.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL072W ; YNL072W ; YNL072W .
GeneIDi 855652.
KEGGi sce:YNL072W.

Organism-specific databases

CYGDi YNL072w.
SGDi S000005016. RNH201.

Phylogenomic databases

eggNOGi COG0164.
GeneTreei ENSGT00390000010768.
HOGENOMi HOG000100290.
InParanoidi P53942.
KOi K10743.
OMAi GPPEKYQ.
OrthoDBi EOG76X68V.

Enzyme and pathway databases

BioCyci YEAST:G3O-33101-MONOMER.

Miscellaneous databases

NextBioi 979902.
PROi P53942.

Gene expression databases

Genevestigatori P53942.

Family and domain databases

Gene3Di 1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProi IPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view ]
PANTHERi PTHR10954. PTHR10954. 1 hit.
Pfami PF01351. RNase_HII. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00729. TIGR00729. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
    Poehlmann R., Philippsen P.
    Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII."
    Frank P., Braunshofer-Reiter C., Wintersberger U.
    FEBS Lett. 421:23-26(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "RNase H2 of Saccharomyces cerevisiae is a complex of three proteins."
    Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
    Nucleic Acids Res. 32:407-414(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RNH202 AND RNH203, MUTAGENESIS OF ASP-39; ASP-155 AND ASP-183.
  8. Erratum
    Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
    Nucleic Acids Res. 32:1616-1616(2004)

Entry informationi

Entry nameiRNH2A_YEAST
AccessioniPrimary (citable) accession number: P53942
Secondary accession number(s): D6W1A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3