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P53942 (RNH2A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease H2 subunit A

Short name=RNase H2 subunit A
EC=3.1.26.4
Alternative name(s):
RNase H(201)
RNase H(35)
Ribonuclease HI large subunit
Short name=RNase HI large subunit
Ribonuclease HI subunit A
Gene names
Name:RNH201
Synonyms:RNH35
Ordered Locus Names:YNL072W
ORF Names:N2369
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactor

Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.

Subunit structure

The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RNase HII family. Eukaryotic subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RNH202Q056353EBI-15663,EBI-33940
RNH203Q123382EBI-15663,EBI-33805

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Ribonuclease H2 subunit A
PRO_0000111718

Sites

Metal binding391Divalent metal cation
Metal binding401Divalent metal cation By similarity
Metal binding1551Divalent metal cation

Experimental info

Mutagenesis391D → A: Abolishes enzyme activity. Ref.7
Mutagenesis1551D → A: Abolishes enzyme activity. Ref.7
Mutagenesis1831D → A: Strongly impairs enzyme activity. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P53942 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 60B1005F674ECC88

FASTA30734,875
        10         20         30         40         50         60 
MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM VYAVAYSTQK 

        70         80         90        100        110        120 
YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI GYATTCITPL DISRGMSKFP 

       130        140        150        160        170        180 
PTRNYNLNEQ AHDVTMALID GVIKQNVKLS HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK 

       190        200        210        220        230        240 
KADSLYCMVS VASVVAKVTR DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP 

       250        260        270        280        290        300 
ANMVRFSWQT CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR 


TLDNWYR 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
Poehlmann R., Philippsen P.
Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII."
Frank P., Braunshofer-Reiter C., Wintersberger U.
FEBS Lett. 421:23-26(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"RNase H2 of Saccharomyces cerevisiae is a complex of three proteins."
Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
Nucleic Acids Res. 32:407-414(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RNH202 AND RNH203, MUTAGENESIS OF ASP-39; ASP-155 AND ASP-183.
[8]Erratum
Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
Nucleic Acids Res. 32:1616-1616(2004)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86470 Genomic DNA. Translation: CAA60188.1.
Z71348 Genomic DNA. Translation: CAA95946.1.
BK006947 Genomic DNA. Translation: DAA10473.1.
PIRS53908.
RefSeqNP_014327.1. NM_001182910.1.

3D structure databases

ProteinModelPortalP53942.
SMRP53942. Positions 20-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35751. 65 interactions.
DIPDIP-4565N.
IntActP53942. 2 interactions.
MINTMINT-532801.
STRING4932.YNL072W.

Proteomic databases

PaxDbP53942.
PeptideAtlasP53942.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL072W; YNL072W; YNL072W.
GeneID855652.
KEGGsce:YNL072W.

Organism-specific databases

CYGDYNL072w.
SGDS000005016. RNH201.

Phylogenomic databases

eggNOGCOG0164.
GeneTreeENSGT00390000010768.
HOGENOMHOG000100290.
KOK10743.
OMAGPPEKYQ.
OrthoDBEOG76X68V.

Enzyme and pathway databases

BioCycYEAST:G3O-33101-MONOMER.

Gene expression databases

GenevestigatorP53942.

Family and domain databases

Gene3D1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERPTHR10954. PTHR10954. 1 hit.
PfamPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00729. TIGR00729. 1 hit.
ProtoNetSearch...

Other

NextBio979902.
PROP53942.

Entry information

Entry nameRNH2A_YEAST
AccessionPrimary (citable) accession number: P53942
Secondary accession number(s): D6W1A7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families