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P53942

- RNH2A_YEAST

UniProt

P53942 - RNH2A_YEAST

Protein

Ribonuclease H2 subunit A

Gene

RNH201

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi39 – 391Divalent metal cation
    Metal bindingi40 – 401Divalent metal cationBy similarity
    Metal bindingi155 – 1551Divalent metal cation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. RNA binding Source: InterPro
    4. RNA-DNA hybrid ribonuclease activity Source: SGD

    GO - Biological processi

    1. DNA replication, removal of RNA primer Source: SGD
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33101-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease H2 subunit A (EC:3.1.26.4)
    Short name:
    RNase H2 subunit A
    Alternative name(s):
    RNase H(201)
    RNase H(35)
    Ribonuclease HI large subunit
    Short name:
    RNase HI large subunit
    Ribonuclease HI subunit A
    Gene namesi
    Name:RNH201
    Synonyms:RNH35
    Ordered Locus Names:YNL072W
    ORF Names:N2369
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL072w.
    SGDiS000005016. RNH201.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD
    3. ribonuclease H2 complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi39 – 391D → A: Abolishes enzyme activity. 1 Publication
    Mutagenesisi155 – 1551D → A: Abolishes enzyme activity. 1 Publication
    Mutagenesisi183 – 1831D → A: Strongly impairs enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307Ribonuclease H2 subunit APRO_0000111718Add
    BLAST

    Proteomic databases

    MaxQBiP53942.
    PaxDbiP53942.
    PeptideAtlasiP53942.

    Expressioni

    Gene expression databases

    GenevestigatoriP53942.

    Interactioni

    Subunit structurei

    The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RNH202Q056353EBI-15663,EBI-33940
    RNH203Q123382EBI-15663,EBI-33805

    Protein-protein interaction databases

    BioGridi35751. 65 interactions.
    DIPiDIP-4565N.
    IntActiP53942. 2 interactions.
    MINTiMINT-532801.
    STRINGi4932.YNL072W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53942.
    SMRiP53942. Positions 20-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNase HII family. Eukaryotic subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0164.
    GeneTreeiENSGT00390000010768.
    HOGENOMiHOG000100290.
    KOiK10743.
    OMAiGPPEKYQ.
    OrthoDBiEOG76X68V.

    Family and domain databases

    Gene3Di1.10.10.460. 1 hit.
    3.30.420.10. 1 hit.
    InterProiIPR004649. RNase_H2_suA.
    IPR001352. RNase_HII/HIII.
    IPR024567. RNase_HII/HIII_dom.
    IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PANTHERiPTHR10954. PTHR10954. 1 hit.
    PfamiPF01351. RNase_HII. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00729. TIGR00729. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53942-1 [UniParc]FASTAAdd to Basket

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    MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM    50
    VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI 100
    GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS 150
    HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR 200
    DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT 250
    CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR 300
    TLDNWYR 307
    Length:307
    Mass (Da):34,875
    Last modified:October 1, 1996 - v1
    Checksum:i60B1005F674ECC88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60188.1.
    Z71348 Genomic DNA. Translation: CAA95946.1.
    BK006947 Genomic DNA. Translation: DAA10473.1.
    PIRiS53908.
    RefSeqiNP_014327.1. NM_001182910.1.

    Genome annotation databases

    EnsemblFungiiYNL072W; YNL072W; YNL072W.
    GeneIDi855652.
    KEGGisce:YNL072W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60188.1 .
    Z71348 Genomic DNA. Translation: CAA95946.1 .
    BK006947 Genomic DNA. Translation: DAA10473.1 .
    PIRi S53908.
    RefSeqi NP_014327.1. NM_001182910.1.

    3D structure databases

    ProteinModelPortali P53942.
    SMRi P53942. Positions 20-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35751. 65 interactions.
    DIPi DIP-4565N.
    IntActi P53942. 2 interactions.
    MINTi MINT-532801.
    STRINGi 4932.YNL072W.

    Proteomic databases

    MaxQBi P53942.
    PaxDbi P53942.
    PeptideAtlasi P53942.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL072W ; YNL072W ; YNL072W .
    GeneIDi 855652.
    KEGGi sce:YNL072W.

    Organism-specific databases

    CYGDi YNL072w.
    SGDi S000005016. RNH201.

    Phylogenomic databases

    eggNOGi COG0164.
    GeneTreei ENSGT00390000010768.
    HOGENOMi HOG000100290.
    KOi K10743.
    OMAi GPPEKYQ.
    OrthoDBi EOG76X68V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33101-MONOMER.

    Miscellaneous databases

    NextBioi 979902.
    PROi P53942.

    Gene expression databases

    Genevestigatori P53942.

    Family and domain databases

    Gene3Di 1.10.10.460. 1 hit.
    3.30.420.10. 1 hit.
    InterProi IPR004649. RNase_H2_suA.
    IPR001352. RNase_HII/HIII.
    IPR024567. RNase_HII/HIII_dom.
    IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    PANTHERi PTHR10954. PTHR10954. 1 hit.
    Pfami PF01351. RNase_HII. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00729. TIGR00729. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
      Poehlmann R., Philippsen P.
      Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII."
      Frank P., Braunshofer-Reiter C., Wintersberger U.
      FEBS Lett. 421:23-26(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "RNase H2 of Saccharomyces cerevisiae is a complex of three proteins."
      Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
      Nucleic Acids Res. 32:407-414(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RNH202 AND RNH203, MUTAGENESIS OF ASP-39; ASP-155 AND ASP-183.
    8. Erratum
      Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
      Nucleic Acids Res. 32:1616-1616(2004)

    Entry informationi

    Entry nameiRNH2A_YEAST
    AccessioniPrimary (citable) accession number: P53942
    Secondary accession number(s): D6W1A7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 623 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3