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P53939

- NIS1_YEAST

UniProt

P53939 - NIS1_YEAST

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Protein

Protein NIS1

Gene

NIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in a mitotic signaling network. Binds sumoylated proteins and may stabilize SUMO chains.1 Publication

GO - Biological processi

  1. regulation of mitosis Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33107-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NIS1
Alternative name(s):
Jumonji domain-interacting protein 1
Neck protein interacting with septins protein 1
Gene namesi
Name:NIS1
Synonyms:JIP1
Ordered Locus Names:YNL078W
ORF Names:N2337
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL078w.
SGDiS000005022. NIS1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cytoplasm Source: UniProtKB-KW
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Protein NIS1PRO_0000203447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei260 – 2601Phosphoserine1 Publication
Modified residuei264 – 2641Phosphoserine1 Publication
Modified residuei300 – 3001Phosphoserine1 Publication
Modified residuei302 – 3021Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53939.
PaxDbiP53939.

Expressioni

Inductioni

Expression is regulated by the ACE2 and SWI5 transcription factors.1 Publication

Gene expression databases

GenevestigatoriP53939.

Interactioni

Subunit structurei

Interacts with CBF2, GIS1, NAP1, PRM8, REI1, SHS1 and SMT3.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NAP1P252936EBI-28760,EBI-11850
NBA1Q082293EBI-28760,EBI-36841

Protein-protein interaction databases

BioGridi35745. 46 interactions.
DIPiDIP-1980N.
IntActiP53939. 15 interactions.
MINTiMINT-397951.
STRINGi4932.YNL078W.

Structurei

3D structure databases

ProteinModelPortaliP53939.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi391 – 3988SUMO-binding

Phylogenomic databases

eggNOGiNOG77367.
InParanoidiP53939.
OMAiDDSHITH.
OrthoDBiEOG7GQZ86.

Sequencei

Sequence statusi: Complete.

P53939-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METYETSIGT QSYPPTLFPP PLGTGGFTTS GYIHALVDST SNSNSNSNTN
60 70 80 90 100
SNTNSNTNSN SDTKIPIVQI SDDSHITHDS FKPYMEYHDA SHLRNRNISK
110 120 130 140 150
ADQVESTEVM EQFTQWSNYK MRSRSPTINA KPIRHTSQRR TDFTSKNELS
160 170 180 190 200
KFSKNHNFIF HKGFLKRQHS IRREDRQAKV RSRFRSKKEL TSVLNYIELE
210 220 230 240 250
QMDIANVLAS QPVNLHAIRN LTSRDPAVTP IPFLRSQMYA TSSRPPYLRN
260 270 280 290 300
RSISRKLPKS QPGSLPTTMP ATATKTIKQN STTPTTRSVY NKNVGRSNTS
310 320 330 340 350
PSVLYHPKRR GKLNTKSHAR KEQLLLELWR EYLMLVITQR TQLRLTLLCS
360 370 380 390 400
PGSASNESSV CSSNASDLDM SLLSTPSSLF QMAGETKSNP IIIPDSQDDS

ILSSDPF
Length:407
Mass (Da):45,908
Last modified:October 1, 1996 - v1
Checksum:i925C7B6063BCE4F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86470 Genomic DNA. Translation: CAA60180.1.
Z71354 Genomic DNA. Translation: CAA95952.1.
BK006947 Genomic DNA. Translation: DAA10467.1.
PIRiS53900.
RefSeqiNP_014321.3. NM_001182916.3.

Genome annotation databases

EnsemblFungiiYNL078W; YNL078W; YNL078W.
GeneIDi855646.
KEGGisce:YNL078W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86470 Genomic DNA. Translation: CAA60180.1 .
Z71354 Genomic DNA. Translation: CAA95952.1 .
BK006947 Genomic DNA. Translation: DAA10467.1 .
PIRi S53900.
RefSeqi NP_014321.3. NM_001182916.3.

3D structure databases

ProteinModelPortali P53939.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35745. 46 interactions.
DIPi DIP-1980N.
IntActi P53939. 15 interactions.
MINTi MINT-397951.
STRINGi 4932.YNL078W.

Proteomic databases

MaxQBi P53939.
PaxDbi P53939.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL078W ; YNL078W ; YNL078W .
GeneIDi 855646.
KEGGi sce:YNL078W.

Organism-specific databases

CYGDi YNL078w.
SGDi S000005022. NIS1.

Phylogenomic databases

eggNOGi NOG77367.
InParanoidi P53939.
OMAi DDSHITH.
OrthoDBi EOG7GQZ86.

Enzyme and pathway databases

BioCyci YEAST:G3O-33107-MONOMER.

Miscellaneous databases

NextBioi 979884.

Gene expression databases

Genevestigatori P53939.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
    Poehlmann R., Philippsen P.
    Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae."
    Iwase M., Toh-e A.
    Genes Genet. Syst. 76:335-343(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH SHS1 AND NAP1.
  5. "Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
    Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
    Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability."
    Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C.
    Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRM8.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae."
    Iwase M., Toh-e A.
    Cell Struct. Funct. 29:1-15(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH REI1, FUNCTION.
  10. "Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation."
    Montpetit B., Hazbun T.R., Fields S., Hieter P.
    J. Cell Biol. 174:653-663(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBF2.
  11. Cited for: DOMAIN, INTERACTION WITH SUMOYLATED PROTEINS.
  12. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  13. "The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair."
    Tronnersjoe S., Hanefalk C., Balciunas D., Hu G.-Z., Nordberg N., Muren E., Ronne H.
    Mol. Genet. Genomics 277:57-70(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GIS1.
  14. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
    Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
    Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-260; SER-264 AND SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNIS1_YEAST
AccessioniPrimary (citable) accession number: P53939
Secondary accession number(s): D6W1A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3