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P53939

- NIS1_YEAST

UniProt

P53939 - NIS1_YEAST

Protein

Protein NIS1

Gene

NIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May be involved in a mitotic signaling network. Binds sumoylated proteins and may stabilize SUMO chains.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of mitosis Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33107-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NIS1
    Alternative name(s):
    Jumonji domain-interacting protein 1
    Neck protein interacting with septins protein 1
    Gene namesi
    Name:NIS1
    Synonyms:JIP1
    Ordered Locus Names:YNL078W
    ORF Names:N2337
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL078w.
    SGDiS000005022. NIS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB-SubCell
    2. cellular bud neck Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Protein NIS1PRO_0000203447Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei260 – 2601Phosphoserine2 Publications
    Modified residuei264 – 2641Phosphoserine2 Publications
    Modified residuei300 – 3001Phosphoserine2 Publications
    Modified residuei302 – 3021Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53939.
    PaxDbiP53939.

    Expressioni

    Inductioni

    Expression is regulated by the ACE2 and SWI5 transcription factors.1 Publication

    Gene expression databases

    GenevestigatoriP53939.

    Interactioni

    Subunit structurei

    Interacts with CBF2, GIS1, NAP1, PRM8, REI1, SHS1 and SMT3.7 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NAP1P252936EBI-28760,EBI-11850
    NBA1Q082293EBI-28760,EBI-36841

    Protein-protein interaction databases

    BioGridi35745. 45 interactions.
    DIPiDIP-1980N.
    IntActiP53939. 15 interactions.
    MINTiMINT-397951.
    STRINGi4932.YNL078W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53939.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi391 – 3988SUMO-binding

    Phylogenomic databases

    eggNOGiNOG77367.
    OMAiDDSHITH.
    OrthoDBiEOG7GQZ86.

    Sequencei

    Sequence statusi: Complete.

    P53939-1 [UniParc]FASTAAdd to Basket

    « Hide

    METYETSIGT QSYPPTLFPP PLGTGGFTTS GYIHALVDST SNSNSNSNTN    50
    SNTNSNTNSN SDTKIPIVQI SDDSHITHDS FKPYMEYHDA SHLRNRNISK 100
    ADQVESTEVM EQFTQWSNYK MRSRSPTINA KPIRHTSQRR TDFTSKNELS 150
    KFSKNHNFIF HKGFLKRQHS IRREDRQAKV RSRFRSKKEL TSVLNYIELE 200
    QMDIANVLAS QPVNLHAIRN LTSRDPAVTP IPFLRSQMYA TSSRPPYLRN 250
    RSISRKLPKS QPGSLPTTMP ATATKTIKQN STTPTTRSVY NKNVGRSNTS 300
    PSVLYHPKRR GKLNTKSHAR KEQLLLELWR EYLMLVITQR TQLRLTLLCS 350
    PGSASNESSV CSSNASDLDM SLLSTPSSLF QMAGETKSNP IIIPDSQDDS 400
    ILSSDPF 407
    Length:407
    Mass (Da):45,908
    Last modified:October 1, 1996 - v1
    Checksum:i925C7B6063BCE4F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60180.1.
    Z71354 Genomic DNA. Translation: CAA95952.1.
    BK006947 Genomic DNA. Translation: DAA10467.1.
    PIRiS53900.
    RefSeqiNP_014321.3. NM_001182916.3.

    Genome annotation databases

    EnsemblFungiiYNL078W; YNL078W; YNL078W.
    GeneIDi855646.
    KEGGisce:YNL078W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60180.1 .
    Z71354 Genomic DNA. Translation: CAA95952.1 .
    BK006947 Genomic DNA. Translation: DAA10467.1 .
    PIRi S53900.
    RefSeqi NP_014321.3. NM_001182916.3.

    3D structure databases

    ProteinModelPortali P53939.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35745. 45 interactions.
    DIPi DIP-1980N.
    IntActi P53939. 15 interactions.
    MINTi MINT-397951.
    STRINGi 4932.YNL078W.

    Proteomic databases

    MaxQBi P53939.
    PaxDbi P53939.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL078W ; YNL078W ; YNL078W .
    GeneIDi 855646.
    KEGGi sce:YNL078W.

    Organism-specific databases

    CYGDi YNL078w.
    SGDi S000005022. NIS1.

    Phylogenomic databases

    eggNOGi NOG77367.
    OMAi DDSHITH.
    OrthoDBi EOG7GQZ86.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33107-MONOMER.

    Miscellaneous databases

    NextBioi 979884.

    Gene expression databases

    Genevestigatori P53939.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
      Poehlmann R., Philippsen P.
      Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae."
      Iwase M., Toh-e A.
      Genes Genet. Syst. 76:335-343(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH SHS1 AND NAP1.
    5. "Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
      Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
      Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability."
      Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C.
      Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRM8.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae."
      Iwase M., Toh-e A.
      Cell Struct. Funct. 29:1-15(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH REI1, FUNCTION.
    10. "Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation."
      Montpetit B., Hazbun T.R., Fields S., Hieter P.
      J. Cell Biol. 174:653-663(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBF2.
    11. Cited for: DOMAIN, INTERACTION WITH SUMOYLATED PROTEINS.
    12. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    13. "The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair."
      Tronnersjoe S., Hanefalk C., Balciunas D., Hu G.-Z., Nordberg N., Muren E., Ronne H.
      Mol. Genet. Genomics 277:57-70(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GIS1.
    14. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
      Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
      Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
    15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-260; SER-264 AND SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNIS1_YEAST
    AccessioniPrimary (citable) accession number: P53939
    Secondary accession number(s): D6W1A1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 504 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3