P53939 (NIS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NIS1

Alternative name(s):
Jumonji domain-interacting protein 1
Neck protein interacting with septins protein 1

Gene names

Name:	NIS1
Synonyms:	JIP1
Ordered Locus Names:	YNL078W
ORF Names:	N2337

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in a mitotic signaling network. Binds sumoylated proteins and may stabilize SUMO chains. Ref.9
Subunit structure	Interacts with CBF2, GIS1, NAP1, PRM8, REI1, SHS1 and SMT3. Ref.4 Ref.6 Ref.9 Ref.11 Ref.12 Ref.14 Ref.15
Subcellular location	Bud neck. Cytoplasm › cell cortex Ref.4 Ref.7.
Induction	Expression is regulated by the ACE2 and SWI5 transcription factors. Ref.5
Miscellaneous	Present with 504 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of mitosis Inferred from physical interaction Ref.4. Source: SGD
Cellular_component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud neck Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NAP1	P25293	6	EBI-28760,EBI-11850
NBA1	Q08229	3	EBI-28760,EBI-36841

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Protein NIS1		PRO_0000203447
Regions
	Motif	391 – 398	8	SUMO-binding
Amino acid modifications
	Modified residue	299	1	Phosphothreonine Ref.16
	Modified residue	300	1	Phosphoserine Ref.10 Ref.13

Sequences

	Sequence		Length	Mass (Da)
	P53939 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 925C7B6063BCE4F1	FASTA	407	45,908
	10 20 30 40 50 60 METYETSIGT QSYPPTLFPP PLGTGGFTTS GYIHALVDST SNSNSNSNTN SNTNSNTNSN 70 80 90 100 110 120 SDTKIPIVQI SDDSHITHDS FKPYMEYHDA SHLRNRNISK ADQVESTEVM EQFTQWSNYK 130 140 150 160 170 180 MRSRSPTINA KPIRHTSQRR TDFTSKNELS KFSKNHNFIF HKGFLKRQHS IRREDRQAKV 190 200 210 220 230 240 RSRFRSKKEL TSVLNYIELE QMDIANVLAS QPVNLHAIRN LTSRDPAVTP IPFLRSQMYA 250 260 270 280 290 300 TSSRPPYLRN RSISRKLPKS QPGSLPTTMP ATATKTIKQN STTPTTRSVY NKNVGRSNTS 310 320 330 340 350 360 PSVLYHPKRR GKLNTKSHAR KEQLLLELWR EYLMLVITQR TQLRLTLLCS PGSASNESSV 370 380 390 400 CSSNASDLDM SLLSTPSSLF QMAGETKSNP IIIPDSQDDS ILSSDPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs." Poehlmann R., Philippsen P. Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae." Iwase M., Toh-e A. Genes Genet. Syst. 76:335-343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH SHS1 AND NAP1.
[5]	"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p." Doolin M.-T., Johnson A.L., Johnston L.H., Butler G. Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability." Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C. Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PRM8.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae." Iwase M., Toh-e A. Cell Struct. Funct. 29:1-15(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH REI1, FUNCTION.
[10]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300, MASS SPECTROMETRY. Strain: YAL6B.
[11]	"Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation." Montpetit B., Hazbun T.R., Fields S., Hieter P. J. Cell Biol. 174:653-663(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CBF2.
[12]	"Ubiquitin-dependent proteolytic control of SUMO conjugates." Uzunova K., Goettsche K., Miteva M., Weisshaar S.R., Glanemann C., Schnellhardt M., Niessen M., Scheel H., Hofmann K., Johnson E.S., Praefcke G.J.K., Dohmen R.J. J. Biol. Chem. 282:34167-34175(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN, INTERACTION WITH SUMOYLATED PROTEINS.
[13]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300, MASS SPECTROMETRY. Strain: ADR376.
[14]	"The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair." Tronnersjoe S., Hanefalk C., Balciunas D., Hu G.-Z., Nordberg N., Muren E., Ronne H. Mol. Genet. Genomics 277:57-70(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GIS1.
[15]	"Phosphorylation by casein kinase 2 regulates Nap1 localization and function." Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F. Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NAP1, MASS SPECTROMETRY.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-299, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86470 Genomic DNA. Translation: CAA60180.1. Z71354 Genomic DNA. Translation: CAA95952.1. BK006947 Genomic DNA. Translation: DAA10467.1.
PIR	S53900.
RefSeq	NP_014321.3. NM_001182916.3.
3D structure databases
ProteinModelPortal	P53939.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1980N.
IntAct	P53939. 13 interactions.
MINT	MINT-397951.
STRING	4932.YNL078W.
Proteomic databases
PaxDb	P53939.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL078W; YNL078W; YNL078W.
GeneID	855646.
KEGG	sce:YNL078W.
Organism-specific databases
CYGD	YNL078w.
SGD	S000005022. NIS1.
Phylogenomic databases
eggNOG	NOG77367.
OMA	DDSHITH.
OrthoDB	EOG4ZGSP8.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33107-MONOMER.
Gene expression databases
Genevestigator	P53939.
GermOnline	YNL078W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979884.

Entry information

Entry name

NIS1_YEAST

Accession

Primary (citable) accession number: P53939
Secondary accession number(s): D6W1A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program