Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P53939 (NIS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein NIS1
Alternative name(s):
Jumonji domain-interacting protein 1
Neck protein interacting with septins protein 1
Gene names
Name:NIS1
Synonyms:JIP1
Ordered Locus Names:YNL078W
ORF Names:N2337
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in a mitotic signaling network. Binds sumoylated proteins and may stabilize SUMO chains. Ref.9

Subunit structure

Interacts with CBF2, GIS1, NAP1, PRM8, REI1, SHS1 and SMT3. Ref.4 Ref.6 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14

Subcellular location

Bud neck. Cytoplasmcell cortex Ref.4 Ref.7.

Induction

Expression is regulated by the ACE2 and SWI5 transcription factors. Ref.5

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.

Ontologies

Keywords
   Cellular componentCytoplasm
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of mitosis

Inferred from physical interaction Ref.4. Source: SGD

   Cellular_componentcell cortex

Inferred from electronic annotation. Source: UniProtKB-SubCell

cellular bud neck

Inferred from direct assay Ref.4. Source: SGD

nucleus

Inferred from direct assay Ref.4. Source: SGD

   Molecular_functionSUMO binding

Inferred from physical interaction Ref.11. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NAP1P252936EBI-28760,EBI-11850
NBA1Q082293EBI-28760,EBI-36841

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Protein NIS1
PRO_0000203447

Regions

Motif391 – 3988SUMO-binding

Amino acid modifications

Modified residue2601Phosphoserine Ref.16
Modified residue2641Phosphoserine Ref.16
Modified residue3001Phosphoserine Ref.12
Modified residue3021Phosphoserine Ref.16

Sequences

Sequence LengthMass (Da)Tools
P53939 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 925C7B6063BCE4F1

FASTA40745,908
        10         20         30         40         50         60 
METYETSIGT QSYPPTLFPP PLGTGGFTTS GYIHALVDST SNSNSNSNTN SNTNSNTNSN 

        70         80         90        100        110        120 
SDTKIPIVQI SDDSHITHDS FKPYMEYHDA SHLRNRNISK ADQVESTEVM EQFTQWSNYK 

       130        140        150        160        170        180 
MRSRSPTINA KPIRHTSQRR TDFTSKNELS KFSKNHNFIF HKGFLKRQHS IRREDRQAKV 

       190        200        210        220        230        240 
RSRFRSKKEL TSVLNYIELE QMDIANVLAS QPVNLHAIRN LTSRDPAVTP IPFLRSQMYA 

       250        260        270        280        290        300 
TSSRPPYLRN RSISRKLPKS QPGSLPTTMP ATATKTIKQN STTPTTRSVY NKNVGRSNTS 

       310        320        330        340        350        360 
PSVLYHPKRR GKLNTKSHAR KEQLLLELWR EYLMLVITQR TQLRLTLLCS PGSASNESSV 

       370        380        390        400 
CSSNASDLDM SLLSTPSSLF QMAGETKSNP IIIPDSQDDS ILSSDPF 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
Poehlmann R., Philippsen P.
Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae."
Iwase M., Toh-e A.
Genes Genet. Syst. 76:335-343(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH SHS1 AND NAP1.
[5]"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
Mol. Microbiol. 40:422-432(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability."
Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C.
Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRM8.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae."
Iwase M., Toh-e A.
Cell Struct. Funct. 29:1-15(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH REI1, FUNCTION.
[10]"Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation."
Montpetit B., Hazbun T.R., Fields S., Hieter P.
J. Cell Biol. 174:653-663(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CBF2.
[11]"Ubiquitin-dependent proteolytic control of SUMO conjugates."
Uzunova K., Goettsche K., Miteva M., Weisshaar S.R., Glanemann C., Schnellhardt M., Niessen M., Scheel H., Hofmann K., Johnson E.S., Praefcke G.J.K., Dohmen R.J.
J. Biol. Chem. 282:34167-34175(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN, INTERACTION WITH SUMOYLATED PROTEINS.
[12]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[13]"The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair."
Tronnersjoe S., Hanefalk C., Balciunas D., Hu G.-Z., Nordberg N., Muren E., Ronne H.
Mol. Genet. Genomics 277:57-70(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GIS1.
[14]"Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
[15]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-260; SER-264 AND SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86470 Genomic DNA. Translation: CAA60180.1.
Z71354 Genomic DNA. Translation: CAA95952.1.
BK006947 Genomic DNA. Translation: DAA10467.1.
PIRS53900.
RefSeqNP_014321.3. NM_001182916.3.

3D structure databases

ProteinModelPortalP53939.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35745. 45 interactions.
DIPDIP-1980N.
IntActP53939. 15 interactions.
MINTMINT-397951.
STRING4932.YNL078W.

Proteomic databases

PaxDbP53939.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL078W; YNL078W; YNL078W.
GeneID855646.
KEGGsce:YNL078W.

Organism-specific databases

CYGDYNL078w.
SGDS000005022. NIS1.

Phylogenomic databases

eggNOGNOG77367.
OMADDSHITH.
OrthoDBEOG7GQZ86.

Enzyme and pathway databases

BioCycYEAST:G3O-33107-MONOMER.

Gene expression databases

GenevestigatorP53939.

Family and domain databases

ProtoNetSearch...

Other

NextBio979884.

Entry information

Entry nameNIS1_YEAST
AccessionPrimary (citable) accession number: P53939
Secondary accession number(s): D6W1A1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD