P53938 (EOS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-glycosylation protein EOS1

Alternative name(s):
ER-localized and oxidants sensitive protein 1

Gene names

Name:	EOS1
Ordered Locus Names:	YNL080C
ORF Names:	N2327

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in oxidative stress resistance and N-glycosylation. Ref.8
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5 Ref.8.
Post-translational modification	N-glycosylated.
Miscellaneous	Present with 937 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the EOS1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to oxidative stress Inferred from mutant phenotype Ref.8. Source: SGD protein N-linked glycosylation Inferred from mutant phenotype Ref.8. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.8. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	N-glycosylation protein EOS1		PRO_0000203446
Regions
	Topological domain	1 – 136	136	Lumenal Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Topological domain	158 – 167	10	Cytoplasmic Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Topological domain	189 – 195	7	Lumenal Potential
	Transmembrane	196 – 216	21	Helical; Potential
	Topological domain	217 – 321	105	Cytoplasmic Potential
	Transmembrane	322 – 342	21	Helical; Potential
	Topological domain	343 – 366	24	Lumenal Potential
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53938 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CFE1BA51A638DF5A	FASTA	366	41,674
	10 20 30 40 50 60 MTWILSTGMG PHEDKYAKHE RATFKKTYSS MKTLSLNHLT AKQHMLMALC RDISLLPPLT 70 80 90 100 110 120 YIFTSLRKAW RVSMRTSITL YEPQSLRDAF TYFWQKLNSA YDNNSSFEGA SQKAVNGDGK 130 140 150 160 170 180 DSLLLSALTT ARASEYLLCS LWCLVSLYLS YAILDSLMVR WIVKYSTVAA ILRMFSMSLI 190 200 210 220 230 240 IVTLELLLLS SLSPELDYFL HTWILISCVL TAVYIWQSYL TSDLRYIRNQ EGEVQEDTNV 250 260 270 280 290 300 PEETEDYEDG EDDADEDSHV VVADESTVDV PSNDSLSDNS DGGLFPVNRP SVSHSQSPKR 310 320 330 340 350 360 PKKYPKKAFN FTTKRTIDLY KITVLCVVPV GLASFITMLG LLRNLFIQRL DVEQLERILH EMHPPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SK1.
[2]	"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs." Poehlmann R., Philippsen P. Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Analysis of deletion phenotypes and GFP fusions of 21 novel Saccharomyces cerevisiae open reading frames." Brachat A., Liebundguth N., Rebischung C., Lemire S., Schaerer F., Hoepfner D., Demchyshyn V., Howald I., Duesterhoeft A., Moestl D., Poehlmann R., Koetter P., Hall M.N., Wach A., Philippsen P. Yeast 16:241-253(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"EOS1, whose deletion confers sensitivity to oxidative stress, is involved in N-glycosylation in Saccharomyces cerevisiae." Nakamura T., Ando A., Takagi H., Shima J. Biochem. Biophys. Res. Commun. 353:293-298(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ115393 Genomic DNA. Translation: AAZ22528.1. X86470 Genomic DNA. Translation: CAA60178.1. Z71356 Genomic DNA. Translation: CAA95954.1. BK006947 Genomic DNA. Translation: DAA10465.1.
PIR	S53898.
RefSeq	NP_014319.1. NM_001182918.1.
3D structure databases
ProteinModelPortal	P53938.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53938. 1 interaction.
STRING	4932.YNL080C.
Proteomic databases
PaxDb	P53938.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL080C; YNL080C; YNL080C.
GeneID	855644.
KEGG	sce:YNL080C.
Organism-specific databases
CYGD	YNL080c.
SGD	S000005024. EOS1.
Phylogenomic databases
eggNOG	NOG46394.
HOGENOM	HOG000248035.
OMA	SLMVRWI.
OrthoDB	EOG4DNJDS.
Gene expression databases
Genevestigator	P53938.
GermOnline	YNL080C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021100. N-glycosylation_EOS1. [Graphical view]
Pfam	PF12326. EOS1. 1 hit. [Graphical view]
PRINTS	PR02070. NGLYCOSEOS1.
ProtoNet	Search...
Other
NextBio	979878.

Entry information

Entry name

EOS1_YEAST

Accession

Primary (citable) accession number: P53938
Secondary accession number(s): D6W199, Q45TY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program