P53935 (NST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stress response protein NST1

Alternative name(s):
Negatively-affecting salt tolerance protein 1

Gene names

Name:	NST1
Ordered Locus Names:	YNL091W
ORF Names:	N2231

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	With MSL1, acts as a negative regulator of salt tolerance. Ref.5
Subunit structure	Interacts with MSL1.
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 217 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NST1 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to salt stress Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAF40	P53829	3	EBI-28788,EBI-28306

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1240	1240	Stress response protein NST1		PRO_0000203442
Regions
	Coiled coil	616 – 777	162	Potential
	Compositional bias	506 – 554	49	His-rich
	Compositional bias	989 – 1115	127	Ser-rich
Amino acid modifications
	Modified residue	266	1	Phosphoserine Ref.8
Natural variations
	Natural variant	162	1	D → A in strain: SK1. Ref.1
	Natural variant	208	1	S → G in strain: SK1. Ref.1
	Natural variant	354	1	E → D in strain: SK1. Ref.1
	Natural variant	899	1	N → D in strain: SK1. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53935 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3FE9D265822D5778	FASTA	1,240	141,514
	10 20 30 40 50 60 MPPNSKSKRR KNKSKQHNKK NGNSDPEQSI NPTQLVPRME PELYHTESDY PTSRVIKRAP 70 80 90 100 110 120 NGDVIVEPIN TDDDKKERTA NLTHNKDSMD SASSLAFTLD SHWESLSPEE KKTILRIEKE 130 140 150 160 170 180 EVFNVIRNYQ DDHSCSCSVC GRRHLAMDQE MERIYNTLYA MDKDKDPETN PIKFHLGIIK 190 200 210 220 230 240 ELQISKNQQQ NDLSSTKGEV VKNFLSSSTV GSLKEEVLHF KQKQLSKQEQ AHNETADNTS 250 260 270 280 290 300 LLEENLNNIH INKTSSEISA NFNSVSDEEL QQKYSNFTKT FISSHPKIAE EYVQKMMMYP 310 320 330 340 350 360 NIRALTDDLM NSNGQGFLNA IEDFVRDGQI QASKKDDSIT EDEASSTDLT DPKEFTTMLH 370 380 390 400 410 420 SGKPLTEDEY ADLQRNIAER MTNAYDTASK KFKDVSQLEK ELFTRFMSGR DKKSFRELII 430 440 450 460 470 480 QSFKNKFDGE LGPSVLAATL SSCFSSQSKD TSLDTDSIYE DEDEEDYDDY SEYAEDSEEV 490 500 510 520 530 540 SEYEGIEAVE KPEHDEKSNG IRETLHLSYD HDHKRQNHPH HHYHSTSTHS EDELSEEEYI 550 560 570 580 590 600 SDIELPHDPH KHFHRDDDIL DGDEDEPEEE DENEGDDEED TYDSGLDETD RLEEGRKLIQ 610 620 630 640 650 660 IAITKLLQSR IMASYHEKQA DNNRLKLLQE LEEEKRKKRE KEEKKQKKRE KEKEKKRLQQ 670 680 690 700 710 720 LAKEEEKRKR EEEKERLKKE LEEREMRRRE AQRKKVEEAK RKKDEERKRR LEEQQRREEM 730 740 750 760 770 780 QEKQRKQKEE LKRKREEEKK RIREQKRLEQ EKLQKEKEEE ERQRLIAEDA LRKQKLNEEQ 790 800 810 820 830 840 TSANILSAKP FTENGVGNPV SSQSHPNMTN YQEDNSCSIN DEILKMVNSV AASKPVSPTG 850 860 870 880 890 900 FNVHDLLLPS TNNQMPAMEQ SHLPQPGNQN NHFGTTTIPN ALDLATKSSL QTENNYLMNS 910 920 930 940 950 960 QTLENTSLLM HNNSSPTKLL PNDFGLSSWG GLTNTMSINP TCKPPVIQTS EMESQAHKSS 970 980 990 1000 1010 1020 PQATMPSFGL PNGGTHRKSF TDELNTLTSM LSSSGFADTS LSSSGFPPSQ RSVWNDQKSS 1030 1040 1050 1060 1070 1080 FSGPSTAGNF NNSSIQSGML LAPTLGSVES FPNRTSIWDS STTPMMNKSE LSGRNITSTA 1090 1100 1110 1120 1130 1140 QDSPAFMASN IWSSNSQYNS PYLTSNVLQS PQISSGVDES HILDSIYNTY LAISPQDSLN 1150 1160 1170 1180 1190 1200 PYIAIGTLFQ NLVGLNLDYS TFINKLISMQ GAYNCEFFTD NNGSITHVRF ARQTPAGHSK 1210 1220 1230 1240 GLLNQLFSGL NDPTATPFTS RPHTSTRASF PIASSTTQTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-162; GLY-208; ASP-354 AND ASP-899. Strain: SK1.
[2]	"Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames." Garcia-Cantalejo J.M., Boskovic J., Jimenez A. Yeast 12:599-608(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Involvement of Nst1p/YNL091w and Msl1p, a U2B'' splicing factor, in Saccharomyces cerevisiae salt tolerance." Goossens A., Forment J., Serrano R. Yeast 19:193-202(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ115393 Genomic DNA. Translation: AAZ22517.1. X85811 Genomic DNA. Translation: CAA59826.1. Z71367 Genomic DNA. Translation: CAA95967.1. BK006947 Genomic DNA. Translation: DAA10455.1.
PIR	S52734.
RefSeq	NP_014308.1. NM_001182929.1.
3D structure databases
ProteinModelPortal	P53935.
SMR	P53935. Positions 630-761.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-872N.
IntAct	P53935. 10 interactions.
MINT	MINT-387884.
STRING	4932.YNL091W.
Proteomic databases
PaxDb	P53935.
PeptideAtlas	P53935.
PRIDE	P53935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL091W; YNL091W; YNL091W.
GeneID	855633.
KEGG	sce:YNL091W.
Organism-specific databases
CYGD	YNL091w.
SGD	S000005035. NST1.
Phylogenomic databases
eggNOG	NOG121137.
GeneTree	ENSGT00700000104144.
HOGENOM	HOG000113867.
OMA	SHWESLS.
OrthoDB	EOG4CC78J.
Gene expression databases
Genevestigator	P53935.
GermOnline	YNL091W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR025279. NST1. [Graphical view]
Pfam	PF13945. NST1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979848.

Entry information

Entry name

NST1_YEAST

Accession

Primary (citable) accession number: P53935
Secondary accession number(s): D6W189, Q45TZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program