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P53935

- NST1_YEAST

UniProt

P53935 - NST1_YEAST

Protein

Stress response protein NST1

Gene

NST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    With MSL1, acts as a negative regulator of salt tolerance.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. response to salt stress Source: SGD

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33119-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Stress response protein NST1
    Alternative name(s):
    Negatively-affecting salt tolerance protein 1
    Gene namesi
    Name:NST1
    Ordered Locus Names:YNL091W
    ORF Names:N2231
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL091w.
    SGDiS000005035. NST1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12401240Stress response protein NST1PRO_0000203442Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei266 – 2661Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53935.
    PaxDbiP53935.
    PeptideAtlasiP53935.
    PRIDEiP53935.

    Expressioni

    Gene expression databases

    GenevestigatoriP53935.

    Interactioni

    Subunit structurei

    Interacts with MSL1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAF40P538293EBI-28788,EBI-28306

    Protein-protein interaction databases

    BioGridi35733. 172 interactions.
    DIPiDIP-872N.
    IntActiP53935. 11 interactions.
    MINTiMINT-387884.
    STRINGi4932.YNL091W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53935.
    SMRiP53935. Positions 630-761.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili616 – 777162Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi506 – 55449His-richAdd
    BLAST
    Compositional biasi989 – 1115127Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NST1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG121137.
    HOGENOMiHOG000113867.
    OMAiSHWESLS.
    OrthoDBiEOG71CFWZ.

    Family and domain databases

    InterProiIPR025279. NST1.
    [Graphical view]
    PfamiPF13945. NST1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53935-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPNSKSKRR KNKSKQHNKK NGNSDPEQSI NPTQLVPRME PELYHTESDY     50
    PTSRVIKRAP NGDVIVEPIN TDDDKKERTA NLTHNKDSMD SASSLAFTLD 100
    SHWESLSPEE KKTILRIEKE EVFNVIRNYQ DDHSCSCSVC GRRHLAMDQE 150
    MERIYNTLYA MDKDKDPETN PIKFHLGIIK ELQISKNQQQ NDLSSTKGEV 200
    VKNFLSSSTV GSLKEEVLHF KQKQLSKQEQ AHNETADNTS LLEENLNNIH 250
    INKTSSEISA NFNSVSDEEL QQKYSNFTKT FISSHPKIAE EYVQKMMMYP 300
    NIRALTDDLM NSNGQGFLNA IEDFVRDGQI QASKKDDSIT EDEASSTDLT 350
    DPKEFTTMLH SGKPLTEDEY ADLQRNIAER MTNAYDTASK KFKDVSQLEK 400
    ELFTRFMSGR DKKSFRELII QSFKNKFDGE LGPSVLAATL SSCFSSQSKD 450
    TSLDTDSIYE DEDEEDYDDY SEYAEDSEEV SEYEGIEAVE KPEHDEKSNG 500
    IRETLHLSYD HDHKRQNHPH HHYHSTSTHS EDELSEEEYI SDIELPHDPH 550
    KHFHRDDDIL DGDEDEPEEE DENEGDDEED TYDSGLDETD RLEEGRKLIQ 600
    IAITKLLQSR IMASYHEKQA DNNRLKLLQE LEEEKRKKRE KEEKKQKKRE 650
    KEKEKKRLQQ LAKEEEKRKR EEEKERLKKE LEEREMRRRE AQRKKVEEAK 700
    RKKDEERKRR LEEQQRREEM QEKQRKQKEE LKRKREEEKK RIREQKRLEQ 750
    EKLQKEKEEE ERQRLIAEDA LRKQKLNEEQ TSANILSAKP FTENGVGNPV 800
    SSQSHPNMTN YQEDNSCSIN DEILKMVNSV AASKPVSPTG FNVHDLLLPS 850
    TNNQMPAMEQ SHLPQPGNQN NHFGTTTIPN ALDLATKSSL QTENNYLMNS 900
    QTLENTSLLM HNNSSPTKLL PNDFGLSSWG GLTNTMSINP TCKPPVIQTS 950
    EMESQAHKSS PQATMPSFGL PNGGTHRKSF TDELNTLTSM LSSSGFADTS 1000
    LSSSGFPPSQ RSVWNDQKSS FSGPSTAGNF NNSSIQSGML LAPTLGSVES 1050
    FPNRTSIWDS STTPMMNKSE LSGRNITSTA QDSPAFMASN IWSSNSQYNS 1100
    PYLTSNVLQS PQISSGVDES HILDSIYNTY LAISPQDSLN PYIAIGTLFQ 1150
    NLVGLNLDYS TFINKLISMQ GAYNCEFFTD NNGSITHVRF ARQTPAGHSK 1200
    GLLNQLFSGL NDPTATPFTS RPHTSTRASF PIASSTTQTS 1240
    Length:1,240
    Mass (Da):141,514
    Last modified:October 1, 1996 - v1
    Checksum:i3FE9D265822D5778
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti162 – 1621D → A in strain: SK1. 1 Publication
    Natural varianti208 – 2081S → G in strain: SK1. 1 Publication
    Natural varianti354 – 3541E → D in strain: SK1. 1 Publication
    Natural varianti899 – 8991N → D in strain: SK1. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115393 Genomic DNA. Translation: AAZ22517.1.
    X85811 Genomic DNA. Translation: CAA59826.1.
    Z71367 Genomic DNA. Translation: CAA95967.1.
    BK006947 Genomic DNA. Translation: DAA10455.1.
    PIRiS52734.
    RefSeqiNP_014308.1. NM_001182929.1.

    Genome annotation databases

    EnsemblFungiiYNL091W; YNL091W; YNL091W.
    GeneIDi855633.
    KEGGisce:YNL091W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115393 Genomic DNA. Translation: AAZ22517.1 .
    X85811 Genomic DNA. Translation: CAA59826.1 .
    Z71367 Genomic DNA. Translation: CAA95967.1 .
    BK006947 Genomic DNA. Translation: DAA10455.1 .
    PIRi S52734.
    RefSeqi NP_014308.1. NM_001182929.1.

    3D structure databases

    ProteinModelPortali P53935.
    SMRi P53935. Positions 630-761.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35733. 172 interactions.
    DIPi DIP-872N.
    IntActi P53935. 11 interactions.
    MINTi MINT-387884.
    STRINGi 4932.YNL091W.

    Proteomic databases

    MaxQBi P53935.
    PaxDbi P53935.
    PeptideAtlasi P53935.
    PRIDEi P53935.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL091W ; YNL091W ; YNL091W .
    GeneIDi 855633.
    KEGGi sce:YNL091W.

    Organism-specific databases

    CYGDi YNL091w.
    SGDi S000005035. NST1.

    Phylogenomic databases

    eggNOGi NOG121137.
    HOGENOMi HOG000113867.
    OMAi SHWESLS.
    OrthoDBi EOG71CFWZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33119-MONOMER.

    Miscellaneous databases

    NextBioi 979848.

    Gene expression databases

    Genevestigatori P53935.

    Family and domain databases

    InterProi IPR025279. NST1.
    [Graphical view ]
    Pfami PF13945. NST1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
      Deutschbauer A.M., Davis R.W.
      Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-162; GLY-208; ASP-354 AND ASP-899.
      Strain: SK1.
    2. "Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames."
      Garcia-Cantalejo J.M., Boskovic J., Jimenez A.
      Yeast 12:599-608(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Involvement of Nst1p/YNL091w and Msl1p, a U2B'' splicing factor, in Saccharomyces cerevisiae salt tolerance."
      Goossens A., Forment J., Serrano R.
      Yeast 19:193-202(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNST1_YEAST
    AccessioniPrimary (citable) accession number: P53935
    Secondary accession number(s): D6W189, Q45TZ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 217 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3