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P53935 (NST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Stress response protein NST1
Alternative name(s):
Negatively-affecting salt tolerance protein 1
Gene names
Name:NST1
Ordered Locus Names:YNL091W
ORF Names:N2231
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1240 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

With MSL1, acts as a negative regulator of salt tolerance. Ref.5

Subunit structure

Interacts with MSL1.

Subcellular location

Cytoplasm Ref.6.

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NST1 family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to salt stress

Inferred from mutant phenotype Ref.5. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CAF40P538293EBI-28788,EBI-28306

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12401240Stress response protein NST1
PRO_0000203442

Regions

Coiled coil616 – 777162 Potential
Compositional bias506 – 55449His-rich
Compositional bias989 – 1115127Ser-rich

Amino acid modifications

Modified residue2661Phosphoserine Ref.9 Ref.10

Natural variations

Natural variant1621D → A in strain: SK1. Ref.1
Natural variant2081S → G in strain: SK1. Ref.1
Natural variant3541E → D in strain: SK1. Ref.1
Natural variant8991N → D in strain: SK1. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53935 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3FE9D265822D5778

FASTA1,240141,514
        10         20         30         40         50         60 
MPPNSKSKRR KNKSKQHNKK NGNSDPEQSI NPTQLVPRME PELYHTESDY PTSRVIKRAP 

        70         80         90        100        110        120 
NGDVIVEPIN TDDDKKERTA NLTHNKDSMD SASSLAFTLD SHWESLSPEE KKTILRIEKE 

       130        140        150        160        170        180 
EVFNVIRNYQ DDHSCSCSVC GRRHLAMDQE MERIYNTLYA MDKDKDPETN PIKFHLGIIK 

       190        200        210        220        230        240 
ELQISKNQQQ NDLSSTKGEV VKNFLSSSTV GSLKEEVLHF KQKQLSKQEQ AHNETADNTS 

       250        260        270        280        290        300 
LLEENLNNIH INKTSSEISA NFNSVSDEEL QQKYSNFTKT FISSHPKIAE EYVQKMMMYP 

       310        320        330        340        350        360 
NIRALTDDLM NSNGQGFLNA IEDFVRDGQI QASKKDDSIT EDEASSTDLT DPKEFTTMLH 

       370        380        390        400        410        420 
SGKPLTEDEY ADLQRNIAER MTNAYDTASK KFKDVSQLEK ELFTRFMSGR DKKSFRELII 

       430        440        450        460        470        480 
QSFKNKFDGE LGPSVLAATL SSCFSSQSKD TSLDTDSIYE DEDEEDYDDY SEYAEDSEEV 

       490        500        510        520        530        540 
SEYEGIEAVE KPEHDEKSNG IRETLHLSYD HDHKRQNHPH HHYHSTSTHS EDELSEEEYI 

       550        560        570        580        590        600 
SDIELPHDPH KHFHRDDDIL DGDEDEPEEE DENEGDDEED TYDSGLDETD RLEEGRKLIQ 

       610        620        630        640        650        660 
IAITKLLQSR IMASYHEKQA DNNRLKLLQE LEEEKRKKRE KEEKKQKKRE KEKEKKRLQQ 

       670        680        690        700        710        720 
LAKEEEKRKR EEEKERLKKE LEEREMRRRE AQRKKVEEAK RKKDEERKRR LEEQQRREEM 

       730        740        750        760        770        780 
QEKQRKQKEE LKRKREEEKK RIREQKRLEQ EKLQKEKEEE ERQRLIAEDA LRKQKLNEEQ 

       790        800        810        820        830        840 
TSANILSAKP FTENGVGNPV SSQSHPNMTN YQEDNSCSIN DEILKMVNSV AASKPVSPTG 

       850        860        870        880        890        900 
FNVHDLLLPS TNNQMPAMEQ SHLPQPGNQN NHFGTTTIPN ALDLATKSSL QTENNYLMNS 

       910        920        930        940        950        960 
QTLENTSLLM HNNSSPTKLL PNDFGLSSWG GLTNTMSINP TCKPPVIQTS EMESQAHKSS 

       970        980        990       1000       1010       1020 
PQATMPSFGL PNGGTHRKSF TDELNTLTSM LSSSGFADTS LSSSGFPPSQ RSVWNDQKSS 

      1030       1040       1050       1060       1070       1080 
FSGPSTAGNF NNSSIQSGML LAPTLGSVES FPNRTSIWDS STTPMMNKSE LSGRNITSTA 

      1090       1100       1110       1120       1130       1140 
QDSPAFMASN IWSSNSQYNS PYLTSNVLQS PQISSGVDES HILDSIYNTY LAISPQDSLN 

      1150       1160       1170       1180       1190       1200 
PYIAIGTLFQ NLVGLNLDYS TFINKLISMQ GAYNCEFFTD NNGSITHVRF ARQTPAGHSK 

      1210       1220       1230       1240 
GLLNQLFSGL NDPTATPFTS RPHTSTRASF PIASSTTQTS 

« Hide

References

« Hide 'large scale' references
[1]"Quantitative trait loci mapped to single-nucleotide resolution in yeast."
Deutschbauer A.M., Davis R.W.
Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-162; GLY-208; ASP-354 AND ASP-899.
Strain: SK1.
[2]"Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames."
Garcia-Cantalejo J.M., Boskovic J., Jimenez A.
Yeast 12:599-608(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Involvement of Nst1p/YNL091w and Msl1p, a U2B'' splicing factor, in Saccharomyces cerevisiae salt tolerance."
Goossens A., Forment J., Serrano R.
Yeast 19:193-202(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ115393 Genomic DNA. Translation: AAZ22517.1.
X85811 Genomic DNA. Translation: CAA59826.1.
Z71367 Genomic DNA. Translation: CAA95967.1.
BK006947 Genomic DNA. Translation: DAA10455.1.
PIRS52734.
RefSeqNP_014308.1. NM_001182929.1.

3D structure databases

ProteinModelPortalP53935.
SMRP53935. Positions 630-761.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35733. 172 interactions.
DIPDIP-872N.
IntActP53935. 11 interactions.
MINTMINT-387884.
STRING4932.YNL091W.

Proteomic databases

PaxDbP53935.
PeptideAtlasP53935.
PRIDEP53935.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL091W; YNL091W; YNL091W.
GeneID855633.
KEGGsce:YNL091W.

Organism-specific databases

CYGDYNL091w.
SGDS000005035. NST1.

Phylogenomic databases

eggNOGNOG121137.
HOGENOMHOG000113867.
OMASHWESLS.
OrthoDBEOG71CFWZ.

Enzyme and pathway databases

BioCycYEAST:G3O-33119-MONOMER.

Gene expression databases

GenevestigatorP53935.

Family and domain databases

InterProIPR025279. NST1.
[Graphical view]
PfamPF13945. NST1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979848.

Entry information

Entry nameNST1_YEAST
AccessionPrimary (citable) accession number: P53935
Secondary accession number(s): D6W189, Q45TZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families