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Protein

Carnosine N-methyltransferase

Gene

YNL092W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

N-methyltransferase that mediates the formation of anserine (beta-alanyl-N(Pi)-methyl-L-histidine) from carnosine. Also methylates other L-histidine-containing di- and tripeptides such as Gly-Gly-His, Gly-His and homocarnosine (GABA-His).1 Publication

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + carnosine = S-adenosyl-L-homocysteine + anserine.1 Publication

Kineticsi

kcat is 0.38 min(-1) for carnosine. kcat is 0.74 min(-1) for S-adenosyl-L-methionine.1 Publication
  1. KM=21.50 mM for carnosine1 Publication
  2. KM=0.002 mM for S-adenosyl-L-methionine1 Publication
  1. Vmax=7.89 nmol/min/mg enzyme with carnosine as substrate1 Publication

pH dependencei

Optimum pH is 7.0-7.5.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

GO - Molecular functioni

  • carnosine N-methyltransferase activity Source: UniProtKB
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

GO - Biological processi

  • carnosine metabolic process Source: UniProtKB
  • protein methylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-33120-MONOMER
ReactomeiR-SCE-70921 Histidine catabolism
SABIO-RKP53934

Names & Taxonomyi

Protein namesi
Recommended name:
Carnosine N-methyltransferase1 Publication (EC:2.1.1.221 Publication)
Gene namesi
Ordered Locus Names:YNL092W
ORF Names:N2227
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL092W
SGDiS000005036 YNL092W

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002034411 – 400Carnosine N-methyltransferaseAdd BLAST400

Proteomic databases

PaxDbiP53934
PRIDEiP53934

Interactioni

Protein-protein interaction databases

BioGridi35732, 37 interactors
DIPiDIP-1880N
IntActiP53934, 3 interactors
MINTiP53934
STRINGi4932.YNL092W

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 23Combined sources15
Helixi25 – 33Combined sources9
Helixi35 – 43Combined sources9
Helixi46 – 51Combined sources6
Helixi55 – 81Combined sources27
Helixi96 – 113Combined sources18
Helixi118 – 135Combined sources18
Beta strandi141 – 145Combined sources5
Helixi151 – 158Combined sources8
Beta strandi162 – 167Combined sources6
Helixi170 – 181Combined sources12
Beta strandi189 – 191Combined sources3
Turni193 – 196Combined sources4
Helixi204 – 208Combined sources5
Beta strandi211 – 213Combined sources3
Beta strandi228 – 233Combined sources6
Helixi235 – 239Combined sources5
Beta strandi242 – 244Combined sources3
Helixi255 – 264Combined sources10
Beta strandi268 – 275Combined sources8
Beta strandi279 – 281Combined sources3
Helixi283 – 293Combined sources11
Beta strandi294 – 304Combined sources11
Turni309 – 312Combined sources4
Beta strandi316 – 320Combined sources5
Beta strandi328 – 331Combined sources4
Beta strandi333 – 338Combined sources6
Helixi345 – 355Combined sources11
Beta strandi358 – 369Combined sources12
Beta strandi385 – 394Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5X62X-ray2.20A/B1-400[»]
ProteinModelPortaliP53934
SMRiP53934
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000005323
HOGENOMiHOG000204444
InParanoidiP53934
KOiK19787
OMAiFDICRAG
OrthoDBiEOG092C4H83

Family and domain databases

InterProiView protein in InterPro
IPR012901 N2227
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12303 PTHR12303, 1 hit
PfamiView protein in Pfam
PF07942 N2227, 1 hit
SMARTiView protein in SMART
SM01296 N2227, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits

Sequencei

Sequence statusi: Complete.

P53934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDENEFDNQR ENKAVARVII SFLKYEEYAL KEIYNLRVKK WASISDRQKD
60 70 80 90 100
MVPNYTKYLA NLKAAIIENG KFFRSVAEYA LQSISFEPGE IVQPNDLDMS
110 120 130 140 150
KTCSLLTQVY REWSAEAISE RNCLNSRLVP FLKTLSPPKA DILIPGCGTG
160 170 180 190 200
RLLVDLSRMG YNCEGNEFSY HMLLVSQYML NAGLLQNQII IYPFIHCFSH
210 220 230 240 250
WKKIEDQLSP IKVPDIEAWS SNKGMGSMSI CAGSFVDCYG RNQGTKISSH
260 270 280 290 300
YTFSRRMQLS RAKAENSKDV VVTNFFIDTG SNILDYLDTI GHVLKPGGIW
310 320 330 340 350
CNFGPLLYHF ENDHGVETTY EVNPYSGFQD KINDYTPLMG LELSSDDIIS
360 370 380 390 400
IATNHLDFEL IRRESGILCG YGRYAGPESC AMPGYMCHYW ILKSNPTNES
Length:400
Mass (Da):45,497
Last modified:October 1, 1996 - v1
Checksum:i6B6C051891F075EE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti42A → E in strain: SK1. 1
Natural varianti154V → M in strain: SK1. 1
Natural varianti317E → D in strain: SK1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85811 Genomic DNA Translation: CAA59825.1
DQ115393 Genomic DNA Translation: AAZ22516.1
Z71368 Genomic DNA Translation: CAA95968.1
BK006947 Genomic DNA Translation: DAA10454.1
PIRiS52733
RefSeqiNP_014307.1, NM_001182930.1

Genome annotation databases

EnsemblFungiiYNL092W; YNL092W; YNL092W
GeneIDi855632
KEGGisce:YNL092W

Similar proteinsi

Entry informationi

Entry nameiCARME_YEAST
AccessioniPrimary (citable) accession number: P53934
Secondary accession number(s): D6W188, Q45TZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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