Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidate phosphatase APP1

Gene

APP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mg2+-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches.1 Publication

Miscellaneous

Present with 2289 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

Inhibited by N-ethylmaleimide.1 Publication

GO - Molecular functioni

  • phosphatidate phosphatase activity Source: SGD

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • cellular lipid metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-33122-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate phosphatase APP1 (EC:3.1.3.4)
Short name:
PAP
Alternative name(s):
Actin patch protein 1
Gene namesi
Name:APP1
Ordered Locus Names:YNL094W
ORF Names:N2219
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL094W.
SGDiS000005038. APP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi281D → E: Abolishes PAP activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000761801 – 587Phosphatidate phosphatase APP1Add BLAST587

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53933.
PRIDEiP53933.

Interactioni

Subunit structurei

Interacts with ABP1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35730. 113 interactors.
DIPiDIP-2006N.
IntActiP53933. 46 interactors.
MINTiMINT-385983.
STRINGi4932.YNL094W.

Structurei

3D structure databases

ProteinModelPortaliP53933.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni467 – 483Interaction with SH3 domain of ABP1Add BLAST17

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi281 – 285DXDXT motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi469 – 475Poly-Pro7

Domaini

Contains one Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.

Phylogenomic databases

HOGENOMiHOG000000857.
InParanoidiP53933.
OMAiIYIRCCK.
OrthoDBiEOG092C0OYH.

Family and domain databases

InterProiView protein in InterPro
IPR017210. APP1.
IPR019236. DUF2183.
PfamiView protein in Pfam
PF09949. DUF2183. 1 hit.
PIRSFiPIRSF037464. UCP037464_APP1. 1 hit.

Sequencei

Sequence statusi: Complete.

P53933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSQGYDESS SSTAATSGPT SGDPRMGKKQ RFMNLIRTTK DVYIPNLTSS
60 70 80 90 100
ISQKTMDGIR STTNSFEGYN DLPMELPHNT TITYFPTYTT TNLVDPDGLS
110 120 130 140 150
APRKDFETTV RCAVSYPGNP TSRRNRWLLS LCKQYLRTGT AEADVAPVVP
160 170 180 190 200
PHLEEDSGDL NDSQSSIESS LSSKSENRYS HMGIQEEDVL NERIQGFLSK
210 220 230 240 250
KVPNTPVVVD LLPKDKLRGD TASFFGTTDS YGNLLIKAET DFLPSKINIT
260 270 280 290 300
LDTPIEGHAD PISETFPANY VSPYGIGLIS DIDDTIKHTG VTGDRRSMFR
310 320 330 340 350
NVFIHDVQSW VIDGVPLWYK TLHDVADVDF FYVSNSPIQT FTLLKQYICA
360 370 380 390 400
NFPPGPIFLK QYSGNFFSTI MTSSANRKIQ PIANILKDFP KKKFILVGDS
410 420 430 440 450
GEHDLEAYTT TALQFPNQIL AIYIRCCSNS MSDVPSHDEE VMNEVNNIIE
460 470 480 490 500
LQQRPMQMTK STVRTRRRPP PPPIPSTQKP SLTEEQTESI RMSRRNKDEN
510 520 530 540 550
NAKRVAPPPL PNRQLPNLDA NTYYVPSSQN DYGMYGAFMD KKADEWKRRV
560 570 580
MDSIQKLSNQ DTTLMFFSDP ALSLEDSIRR IREKYSN
Length:587
Mass (Da):66,134
Last modified:October 1, 1996 - v1
Checksum:iB85C525548BA34BC
GO

Sequence cautioni

The sequence CAA59823 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85811 Genomic DNA. Translation: CAA59823.1. Different initiation.
Z71370 Genomic DNA. Translation: CAA95970.1.
BK006947 Genomic DNA. Translation: DAA10452.1.
PIRiS63033.
RefSeqiNP_014305.1. NM_001182932.1.

Genome annotation databases

EnsemblFungiiYNL094W; YNL094W; YNL094W.
GeneIDi855630.
KEGGisce:YNL094W.

Similar proteinsi

Entry informationi

Entry nameiAPP1_YEAST
AccessioniPrimary (citable) accession number: P53933
Secondary accession number(s): D6W186
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names