Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53933

- APP1_YEAST

UniProt

P53933 - APP1_YEAST

Protein

Phosphatidate phosphatase APP1

Gene

APP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Mg2+-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches.1 Publication

    Catalytic activityi

    A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.1 Publication

    Cofactori

    Mg2+.By similarity

    Enzyme regulationi

    Inhibited by N-ethylmaleimide.1 Publication

    GO - Molecular functioni

    1. phosphatidate phosphatase activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin cytoskeleton organization Source: SGD
    2. cellular lipid metabolic process Source: SGD
    3. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid metabolism

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33122-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidate phosphatase APP1 (EC:3.1.3.4)
    Short name:
    PAP
    Alternative name(s):
    Actin patch protein 1
    Gene namesi
    Name:APP1
    Ordered Locus Names:YNL094W
    ORF Names:N2219
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL094w.
    SGDiS000005038. APP1.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 2 Publications

    GO - Cellular componenti

    1. actin cortical patch Source: SGD
    2. actin cytoskeleton Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi281 – 2811D → E: Abolishes PAP activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 587587Phosphatidate phosphatase APP1PRO_0000076180Add
    BLAST

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP53933.
    PaxDbiP53933.

    Expressioni

    Gene expression databases

    GenevestigatoriP53933.

    Interactioni

    Subunit structurei

    Interacts with ABP1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P158919EBI-28798,EBI-2036
    BBC1P470683EBI-28798,EBI-3437
    BZZ1P3882214EBI-28798,EBI-3889
    HOF1Q050802EBI-28798,EBI-5412
    LSB1P532814EBI-28798,EBI-23329
    LSB3P436038EBI-28798,EBI-22980
    MYO5Q044392EBI-28798,EBI-11687
    PEX13P806672EBI-28798,EBI-13206
    PIN3Q064494EBI-28798,EBI-35523
    RVS167P397439EBI-28798,EBI-14500
    SLA1P327904EBI-28798,EBI-17313
    YSC84P327936EBI-28798,EBI-24460

    Protein-protein interaction databases

    BioGridi35730. 57 interactions.
    DIPiDIP-2006N.
    IntActiP53933. 28 interactions.
    MINTiMINT-385983.
    STRINGi4932.YNL094W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53933.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni467 – 48317Interaction with SH3 domain of ABP1Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi281 – 2855DXDXT motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi469 – 4757Poly-Pro

    Domaini

    Contains one Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.

    Phylogenomic databases

    eggNOGiCOG4850.
    HOGENOMiHOG000000857.
    OMAiSPWQLFS.
    OrthoDBiEOG7P2Z1R.

    Family and domain databases

    InterProiIPR019236. DUF2183.
    IPR017210. UCP037464_APP1.
    [Graphical view]
    PfamiPF09949. DUF2183. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037464. UCP037464_APP1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53933-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSQGYDESS SSTAATSGPT SGDPRMGKKQ RFMNLIRTTK DVYIPNLTSS    50
    ISQKTMDGIR STTNSFEGYN DLPMELPHNT TITYFPTYTT TNLVDPDGLS 100
    APRKDFETTV RCAVSYPGNP TSRRNRWLLS LCKQYLRTGT AEADVAPVVP 150
    PHLEEDSGDL NDSQSSIESS LSSKSENRYS HMGIQEEDVL NERIQGFLSK 200
    KVPNTPVVVD LLPKDKLRGD TASFFGTTDS YGNLLIKAET DFLPSKINIT 250
    LDTPIEGHAD PISETFPANY VSPYGIGLIS DIDDTIKHTG VTGDRRSMFR 300
    NVFIHDVQSW VIDGVPLWYK TLHDVADVDF FYVSNSPIQT FTLLKQYICA 350
    NFPPGPIFLK QYSGNFFSTI MTSSANRKIQ PIANILKDFP KKKFILVGDS 400
    GEHDLEAYTT TALQFPNQIL AIYIRCCSNS MSDVPSHDEE VMNEVNNIIE 450
    LQQRPMQMTK STVRTRRRPP PPPIPSTQKP SLTEEQTESI RMSRRNKDEN 500
    NAKRVAPPPL PNRQLPNLDA NTYYVPSSQN DYGMYGAFMD KKADEWKRRV 550
    MDSIQKLSNQ DTTLMFFSDP ALSLEDSIRR IREKYSN 587
    Length:587
    Mass (Da):66,134
    Last modified:October 1, 1996 - v1
    Checksum:iB85C525548BA34BC
    GO

    Sequence cautioni

    The sequence CAA59823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85811 Genomic DNA. Translation: CAA59823.1. Different initiation.
    Z71370 Genomic DNA. Translation: CAA95970.1.
    BK006947 Genomic DNA. Translation: DAA10452.1.
    PIRiS63033.
    RefSeqiNP_014305.1. NM_001182932.1.

    Genome annotation databases

    EnsemblFungiiYNL094W; YNL094W; YNL094W.
    GeneIDi855630.
    KEGGisce:YNL094W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85811 Genomic DNA. Translation: CAA59823.1 . Different initiation.
    Z71370 Genomic DNA. Translation: CAA95970.1 .
    BK006947 Genomic DNA. Translation: DAA10452.1 .
    PIRi S63033.
    RefSeqi NP_014305.1. NM_001182932.1.

    3D structure databases

    ProteinModelPortali P53933.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35730. 57 interactions.
    DIPi DIP-2006N.
    IntActi P53933. 28 interactions.
    MINTi MINT-385983.
    STRINGi 4932.YNL094W.

    Proteomic databases

    MaxQBi P53933.
    PaxDbi P53933.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL094W ; YNL094W ; YNL094W .
    GeneIDi 855630.
    KEGGi sce:YNL094W.

    Organism-specific databases

    CYGDi YNL094w.
    SGDi S000005038. APP1.

    Phylogenomic databases

    eggNOGi COG4850.
    HOGENOMi HOG000000857.
    OMAi SPWQLFS.
    OrthoDBi EOG7P2Z1R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33122-MONOMER.

    Miscellaneous databases

    NextBioi 979839.

    Gene expression databases

    Genevestigatori P53933.

    Family and domain databases

    InterProi IPR019236. DUF2183.
    IPR017210. UCP037464_APP1.
    [Graphical view ]
    Pfami PF09949. DUF2183. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037464. UCP037464_APP1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames."
      Garcia-Cantalejo J.M., Boskovic J., Jimenez A.
      Yeast 12:599-608(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: INTERACTION WITH ABP1.
    8. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
      Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
      Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
    9. "The Saccharomyces cerevisiae actin patch protein App1p is a phosphatidate phosphatase enzyme."
      Chae M., Han G.S., Carman G.M.
      J. Biol. Chem. 287:40186-40196(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF ASP-281.

    Entry informationi

    Entry nameiAPP1_YEAST
    AccessioniPrimary (citable) accession number: P53933
    Secondary accession number(s): D6W186
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2289 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3