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P53933 (APP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidate phosphatase APP1

Short name=PAP
EC=3.1.3.4
Alternative name(s):
Actin patch protein 1
Gene names
Name:APP1
Ordered Locus Names:YNL094W
ORF Names:N2219
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mg2+-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches. Ref.9

Catalytic activity

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate. Ref.9

Cofactor

Mg2+ By similarity.

Enzyme regulation

Inhibited by N-ethylmaleimide. Ref.9

Subunit structure

Interacts with ABP1. Ref.7

Subcellular location

Cytoplasmcytoskeletonactin patch Ref.4 Ref.5.

Domain

Contains one Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.

Post-translational modification

N-glycosylated.

Miscellaneous

Present with 2289 molecules/cell in log phase SD medium.

Sequence caution

The sequence CAA59823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Phosphatidate phosphatase APP1
PRO_0000076180

Regions

Region467 – 48317Interaction with SH3 domain of ABP1
Motif281 – 2855DXDXT motif
Compositional bias469 – 4757Poly-Pro

Experimental info

Mutagenesis2811D → E: Abolishes PAP activity. Ref.9

Sequences

Sequence LengthMass (Da)Tools
P53933 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B85C525548BA34BC

FASTA58766,134
        10         20         30         40         50         60 
MNSQGYDESS SSTAATSGPT SGDPRMGKKQ RFMNLIRTTK DVYIPNLTSS ISQKTMDGIR 

        70         80         90        100        110        120 
STTNSFEGYN DLPMELPHNT TITYFPTYTT TNLVDPDGLS APRKDFETTV RCAVSYPGNP 

       130        140        150        160        170        180 
TSRRNRWLLS LCKQYLRTGT AEADVAPVVP PHLEEDSGDL NDSQSSIESS LSSKSENRYS 

       190        200        210        220        230        240 
HMGIQEEDVL NERIQGFLSK KVPNTPVVVD LLPKDKLRGD TASFFGTTDS YGNLLIKAET 

       250        260        270        280        290        300 
DFLPSKINIT LDTPIEGHAD PISETFPANY VSPYGIGLIS DIDDTIKHTG VTGDRRSMFR 

       310        320        330        340        350        360 
NVFIHDVQSW VIDGVPLWYK TLHDVADVDF FYVSNSPIQT FTLLKQYICA NFPPGPIFLK 

       370        380        390        400        410        420 
QYSGNFFSTI MTSSANRKIQ PIANILKDFP KKKFILVGDS GEHDLEAYTT TALQFPNQIL 

       430        440        450        460        470        480 
AIYIRCCSNS MSDVPSHDEE VMNEVNNIIE LQQRPMQMTK STVRTRRRPP PPPIPSTQKP 

       490        500        510        520        530        540 
SLTEEQTESI RMSRRNKDEN NAKRVAPPPL PNRQLPNLDA NTYYVPSSQN DYGMYGAFMD 

       550        560        570        580 
KKADEWKRRV MDSIQKLSNQ DTTLMFFSDP ALSLEDSIRR IREKYSN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames."
Garcia-Cantalejo J.M., Boskovic J., Jimenez A.
Yeast 12:599-608(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A protein interaction map for cell polarity development."
Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. expand/collapse author list , Pringle J.R., Davis T.N., Fields S., Drubin D.G.
J. Cell Biol. 154:549-571(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Protein interaction networks by proteome peptide scanning."
Landgraf C., Panni S., Montecchi-Palazzi L., Castagnoli L., Schneider-Mergener J., Volkmer-Engert R., Cesareni G.
PLoS Biol. 2:94-103(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ABP1.
[8]"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
[9]"The Saccharomyces cerevisiae actin patch protein App1p is a phosphatidate phosphatase enzyme."
Chae M., Han G.S., Carman G.M.
J. Biol. Chem. 287:40186-40196(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF ASP-281.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85811 Genomic DNA. Translation: CAA59823.1. Different initiation.
Z71370 Genomic DNA. Translation: CAA95970.1.
BK006947 Genomic DNA. Translation: DAA10452.1.
PIRS63033.
RefSeqNP_014305.1. NM_001182932.1.

3D structure databases

ProteinModelPortalP53933.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35730. 57 interactions.
DIPDIP-2006N.
IntActP53933. 28 interactions.
MINTMINT-385983.
STRING4932.YNL094W.

Proteomic databases

PaxDbP53933.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL094W; YNL094W; YNL094W.
GeneID855630.
KEGGsce:YNL094W.

Organism-specific databases

CYGDYNL094w.
SGDS000005038. APP1.

Phylogenomic databases

eggNOGCOG4850.
HOGENOMHOG000000857.
OMASPWQLFS.
OrthoDBEOG7P2Z1R.

Enzyme and pathway databases

BioCycYEAST:G3O-33122-MONOMER.

Gene expression databases

ArrayExpressP53933.
GenevestigatorP53933.

Family and domain databases

InterProIPR019236. DUF2183.
IPR017210. UCP037464_APP1.
[Graphical view]
PfamPF09949. DUF2183. 1 hit.
[Graphical view]
PIRSFPIRSF037464. UCP037464_APP1. 1 hit.
ProtoNetSearch...

Other

NextBio979839.

Entry information

Entry nameAPP1_YEAST
AccessionPrimary (citable) accession number: P53933
Secondary accession number(s): D6W186
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD