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Protein

Protein AF-9 homolog

Gene

YAF9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex is also involved in DNA repair. Yaf9 may also be required for viability in conditions in which the structural integrity of the spindle is compromised.5 Publications

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • chromatin remodeling Source: SGD
  • chromatin silencing at telomere Source: SGD
  • DNA repair Source: SGD
  • histone exchange Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33132-MONOMER.
BRENDAi2.3.1.48. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AF-9 homolog
Gene namesi
Name:YAF9
Ordered Locus Names:YNL107W
ORF Names:N1966
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL107W.
SGDiS000005051. YAF9.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • cytoplasm Source: UniProtKB-SubCell
  • NuA4 histone acetyltransferase complex Source: SGD
  • Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002159341 – 226Protein AF-9 homologAdd BLAST226

Proteomic databases

MaxQBiP53930.
PRIDEiP53930.

Interactioni

Subunit structurei

Component of the SWR1 chromatin-remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2. Interacts with SWC4.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF1P358173EBI-28841,EBI-3493
EAF1Q063372EBI-28841,EBI-35867
ESA1Q086496EBI-28841,EBI-6648
SWC4P532015EBI-28841,EBI-23061
TRA1P388114EBI-28841,EBI-24638

Protein-protein interaction databases

BioGridi35717. 386 interactors.
DIPiDIP-4347N.
IntActiP53930. 31 interactors.
MINTiMINT-515470.

Structurei

Secondary structure

1226
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 11Combined sources4
Beta strandi14 – 26Combined sources13
Beta strandi41 – 48Combined sources8
Helixi50 – 52Combined sources3
Turni56 – 58Combined sources3
Beta strandi59 – 65Combined sources7
Beta strandi70 – 72Combined sources3
Beta strandi74 – 77Combined sources4
Beta strandi79 – 90Combined sources12
Beta strandi93 – 100Combined sources8
Helixi102 – 104Combined sources3
Beta strandi109 – 114Combined sources6
Beta strandi145 – 157Combined sources13
Helixi160 – 168Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FK3X-ray2.30A/B/C8-169[»]
3RLSX-ray1.70A/B9-176[»]
ProteinModelPortaliP53930.
SMRiP53930.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53930.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 123YEATSPROSITE-ProRule annotationAdd BLAST109

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili187 – 224Sequence analysisAdd BLAST38

Domaini

The coiled-coil domain is required for assembly into the NuA4 complex.

Sequence similaritiesi

Contains 1 YEATS domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000063543.
HOGENOMiHOG000190181.
InParanoidiP53930.
KOiK11341.
OMAiPEHTHEW.
OrthoDBiEOG092C5HF0.

Family and domain databases

InterProiIPR005033. YEATS.
[Graphical view]
PANTHERiPTHR23195. PTHR23195. 1 hit.
PfamiPF03366. YEATS. 1 hit.
[Graphical view]
PROSITEiPS51037. YEATS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPTISKRIK TLSVSRPIIY GNTAKKMGSV KPPNAPAEHT HLWTIFVRGP
60 70 80 90 100
QNEDISYFIK KVVFKLHDTY PNPVRSIEAP PFELTETGWG EFDINIKVYF
110 120 130 140 150
VEEANEKVLN FYHRLRLHPY ANPVPNSDNG NEQNTTDHNS KDAEVSSVYF
160 170 180 190 200
DEIVFNEPNE EFFKILMSRP GNLLPSNKTD DCVYSKQLEQ EEIDRIEIGI
210 220
EKVDKEIDEL KQKLENLVKQ EAINGS
Length:226
Mass (Da):25,981
Last modified:October 1, 1996 - v1
Checksum:i1FE08F72942061D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65K → R in AAT93068 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA. Translation: CAA93400.1.
Z71383 Genomic DNA. Translation: CAA95984.1.
AY693049 Genomic DNA. Translation: AAT93068.1.
BK006947 Genomic DNA. Translation: DAA10439.1.
PIRiS63048.
RefSeqiNP_014292.3. NM_001182945.3.

Genome annotation databases

EnsemblFungiiYNL107W; YNL107W; YNL107W.
GeneIDi855616.
KEGGisce:YNL107W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA. Translation: CAA93400.1.
Z71383 Genomic DNA. Translation: CAA95984.1.
AY693049 Genomic DNA. Translation: AAT93068.1.
BK006947 Genomic DNA. Translation: DAA10439.1.
PIRiS63048.
RefSeqiNP_014292.3. NM_001182945.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FK3X-ray2.30A/B/C8-169[»]
3RLSX-ray1.70A/B9-176[»]
ProteinModelPortaliP53930.
SMRiP53930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35717. 386 interactors.
DIPiDIP-4347N.
IntActiP53930. 31 interactors.
MINTiMINT-515470.

Proteomic databases

MaxQBiP53930.
PRIDEiP53930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL107W; YNL107W; YNL107W.
GeneIDi855616.
KEGGisce:YNL107W.

Organism-specific databases

EuPathDBiFungiDB:YNL107W.
SGDiS000005051. YAF9.

Phylogenomic databases

GeneTreeiENSGT00530000063543.
HOGENOMiHOG000190181.
InParanoidiP53930.
KOiK11341.
OMAiPEHTHEW.
OrthoDBiEOG092C5HF0.

Enzyme and pathway databases

BioCyciYEAST:G3O-33132-MONOMER.
BRENDAi2.3.1.48. 984.

Miscellaneous databases

EvolutionaryTraceiP53930.
PROiP53930.

Family and domain databases

InterProiIPR005033. YEATS.
[Graphical view]
PANTHERiPTHR23195. PTHR23195. 1 hit.
PfamiPF03366. YEATS. 1 hit.
[Graphical view]
PROSITEiPS51037. YEATS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAF9_YEAST
AccessioniPrimary (citable) accession number: P53930
Secondary accession number(s): D6W173, Q6B1N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.