Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosome biogenesis protein 15

Gene

NOP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.2 Publications

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • rRNA binding Source: GO_Central

GO - Biological processi

  • actomyosin contractile ring assembly Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  • ribosomal large subunit biogenesis Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33134-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein 15
Alternative name(s):
Nucleolar protein 151 Publication
Gene namesi
Name:NOP151 Publication
Ordered Locus Names:YNL110C
ORF Names:N1954
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL110C.
SGDiS000005054. NOP15.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleolus Source: SGD
  • nucleus Source: SGD
  • preribosome, large subunit precursor Source: SGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Abrupt growth arrest prior to substantial depletion of ribosomal subunits. Fails to synthesize the 25S and 5.8S rRNA components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA is strongly inhibited. Arrests at cytokinesis and fails to assemble a contractile actin ring at the bud neck.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000820351 – 220Ribosome biogenesis protein 15Add BLAST220

Proteomic databases

MaxQBiP53927.
PRIDEiP53927.

PTM databases

iPTMnetiP53927.

Interactioni

Subunit structurei

Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi35715. 226 interactors.
DIPiDIP-4741N.
IntActiP53927. 53 interactors.
MINTiMINT-551800.
STRINGi4932.YNL110C.

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi89 – 96Combined sources8
Helixi104 – 111Combined sources8
Helixi112 – 114Combined sources3
Beta strandi117 – 124Combined sources8
Turni126 – 128Combined sources3
Beta strandi131 – 141Combined sources11
Helixi142 – 152Combined sources11
Beta strandi163 – 168Combined sources6
Helixi173 – 176Combined sources4
Helixi181 – 186Combined sources6
Turni187 – 190Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08o1-220[»]
5T9PX-ray2.00A/B/C/D81-191[»]
ProteinModelPortaliP53927.
SMRiP53927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 169RRMPROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

GeneTreeiENSGT00390000011515.
HOGENOMiHOG000000855.
InParanoidiP53927.
KOiK14838.
OMAiDEKHEER.
OrthoDBiEOG092C4XOZ.

Family and domain databases

CDDicd12552. RRM_Nop15p. 1 hit.
InterProiView protein in InterPro
IPR034469. Nop15_RRM.
IPR000504. RRM_dom.
PfamiView protein in Pfam
PF00076. RRM_1. 1 hit.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

P53927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI
60 70 80 90 100
EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH
110 120 130 140 150
GFHEKELSKY FAQFGDLKEV RLARNKKTGN SRHYGFLEFV NKEDAMIAQE
160 170 180 190 200
SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK KRVLVEKGIT KPVKQLKDNM
210 220
KQKHEERIKK LAKSGIEFKW
Length:220
Mass (Da):25,447
Last modified:October 1, 1996 - v1
Checksum:iEC86E3C77C863292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA. Translation: CAA93397.1.
Z71386 Genomic DNA. Translation: CAA95989.1.
AY693098 Genomic DNA. Translation: AAT93117.1.
BK006947 Genomic DNA. Translation: DAA10437.1.
PIRiS63051.
RefSeqiNP_014289.1. NM_001182948.1.

Genome annotation databases

EnsemblFungiiYNL110C; YNL110C; YNL110C.
GeneIDi855613.
KEGGisce:YNL110C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNOP15_YEAST
AccessioniPrimary (citable) accession number: P53927
Secondary accession number(s): D6W171
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names