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Protein

Ribosome biogenesis protein 15

Gene

NOP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.2 Publications

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • rRNA binding Source: GO_Central

GO - Biological processi

  • actomyosin contractile ring assembly Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  • ribosomal large subunit biogenesis Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33134-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein 15
Alternative name(s):
Nucleolar protein 151 Publication
Gene namesi
Name:NOP151 Publication
Ordered Locus Names:YNL110C
ORF Names:N1954
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL110C
SGDiS000005054 NOP15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Abrupt growth arrest prior to substantial depletion of ribosomal subunits. Fails to synthesize the 25S and 5.8S rRNA components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA is strongly inhibited. Arrests at cytokinesis and fails to assemble a contractile actin ring at the bud neck.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000820351 – 220Ribosome biogenesis protein 15Add BLAST220

Proteomic databases

MaxQBiP53927
PaxDbiP53927
PRIDEiP53927

PTM databases

iPTMnetiP53927

Interactioni

Subunit structurei

Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35715, 231 interactors
DIPiDIP-4741N
IntActiP53927, 54 interactors
MINTiP53927
STRINGi4932.YNL110C

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi89 – 96Combined sources8
Helixi104 – 111Combined sources8
Helixi112 – 114Combined sources3
Beta strandi117 – 124Combined sources8
Turni126 – 128Combined sources3
Beta strandi131 – 141Combined sources11
Helixi142 – 152Combined sources11
Beta strandi163 – 168Combined sources6
Helixi173 – 176Combined sources4
Helixi181 – 186Combined sources6
Turni187 – 190Combined sources4
Helixi195 – 207Combined sources13
Helixi209 – 213Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08o1-220[»]
5T9PX-ray2.00A/B/C/D81-191[»]
5Z3Gelectron microscopy3.65E1-220[»]
6C0Felectron microscopy3.70o1-220[»]
6CB1electron microscopy4.60o1-220[»]
6ELZelectron microscopy3.30o1-220[»]
6EM1electron microscopy3.60o1-220[»]
6EM3electron microscopy3.20o1-220[»]
6EM4electron microscopy4.10o1-220[»]
6EM5electron microscopy4.30o1-220[»]
ProteinModelPortaliP53927
SMRiP53927
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 169RRMPROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

GeneTreeiENSGT00390000011515
HOGENOMiHOG000000855
InParanoidiP53927
KOiK14838
OMAiDEKHEER
OrthoDBiEOG092C4XOZ

Family and domain databases

CDDicd12552 RRM_Nop15p, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034469 Nop15_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

P53927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI
60 70 80 90 100
EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH
110 120 130 140 150
GFHEKELSKY FAQFGDLKEV RLARNKKTGN SRHYGFLEFV NKEDAMIAQE
160 170 180 190 200
SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK KRVLVEKGIT KPVKQLKDNM
210 220
KQKHEERIKK LAKSGIEFKW
Length:220
Mass (Da):25,447
Last modified:October 1, 1996 - v1
Checksum:iEC86E3C77C863292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA Translation: CAA93397.1
Z71386 Genomic DNA Translation: CAA95989.1
AY693098 Genomic DNA Translation: AAT93117.1
BK006947 Genomic DNA Translation: DAA10437.1
PIRiS63051
RefSeqiNP_014289.1, NM_001182948.1

Genome annotation databases

EnsemblFungiiYNL110C; YNL110C; YNL110C
GeneIDi855613
KEGGisce:YNL110C

Similar proteinsi

Entry informationi

Entry nameiNOP15_YEAST
AccessioniPrimary (citable) accession number: P53927
Secondary accession number(s): D6W171
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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