Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53927

- NOP15_YEAST

UniProt

P53927 - NOP15_YEAST

Protein

Ribosome biogenesis protein 15

Gene

NOP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.2 Publications

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. actomyosin contractile ring assembly Source: SGD
    2. ribosomal large subunit biogenesis Source: SGD
    3. rRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33134-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome biogenesis protein 15
    Alternative name(s):
    Nucleolar protein 15
    Gene namesi
    Name:NOP15
    Ordered Locus Names:YNL110C
    ORF Names:N1954
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL110c.
    SGDiS000005054. NOP15.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: SGD
    3. nucleus Source: SGD
    4. preribosome, large subunit precursor Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Essential gene. Abrupt growth arrest prior to substantial depletion of ribosomal subunits. Fails to synthesize the 25S and 5.8S rRNA components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA is strongly inhibited. Arrests at cytokinesis and fails to assemble a contractile actin ring at the bud neck.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220Ribosome biogenesis protein 15PRO_0000082035Add
    BLAST

    Proteomic databases

    MaxQBiP53927.
    PaxDbiP53927.
    PeptideAtlasiP53927.

    Expressioni

    Gene expression databases

    GenevestigatoriP53927.

    Interactioni

    Subunit structurei

    Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CIC1P387794EBI-28853,EBI-24538

    Protein-protein interaction databases

    BioGridi35715. 222 interactions.
    DIPiDIP-4741N.
    IntActiP53927. 51 interactions.
    MINTiMINT-551800.
    STRINGi4932.YNL110C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53927.
    SMRiP53927. Positions 59-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 16979RRMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG244773.
    GeneTreeiENSGT00390000011515.
    HOGENOMiHOG000000855.
    KOiK14838.
    OMAiKGFHERE.
    OrthoDBiEOG747PVB.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI    50
    EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH 100
    GFHEKELSKY FAQFGDLKEV RLARNKKTGN SRHYGFLEFV NKEDAMIAQE 150
    SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK KRVLVEKGIT KPVKQLKDNM 200
    KQKHEERIKK LAKSGIEFKW 220
    Length:220
    Mass (Da):25,447
    Last modified:October 1, 1996 - v1
    Checksum:iEC86E3C77C863292
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69382 Genomic DNA. Translation: CAA93397.1.
    Z71386 Genomic DNA. Translation: CAA95989.1.
    AY693098 Genomic DNA. Translation: AAT93117.1.
    BK006947 Genomic DNA. Translation: DAA10437.1.
    PIRiS63051.
    RefSeqiNP_014289.1. NM_001182948.1.

    Genome annotation databases

    EnsemblFungiiYNL110C; YNL110C; YNL110C.
    GeneIDi855613.
    KEGGisce:YNL110C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69382 Genomic DNA. Translation: CAA93397.1 .
    Z71386 Genomic DNA. Translation: CAA95989.1 .
    AY693098 Genomic DNA. Translation: AAT93117.1 .
    BK006947 Genomic DNA. Translation: DAA10437.1 .
    PIRi S63051.
    RefSeqi NP_014289.1. NM_001182948.1.

    3D structure databases

    ProteinModelPortali P53927.
    SMRi P53927. Positions 59-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35715. 222 interactions.
    DIPi DIP-4741N.
    IntActi P53927. 51 interactions.
    MINTi MINT-551800.
    STRINGi 4932.YNL110C.

    Proteomic databases

    MaxQBi P53927.
    PaxDbi P53927.
    PeptideAtlasi P53927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL110C ; YNL110C ; YNL110C .
    GeneIDi 855613.
    KEGGi sce:YNL110C.

    Organism-specific databases

    CYGDi YNL110c.
    SGDi S000005054. NOP15.

    Phylogenomic databases

    eggNOGi NOG244773.
    GeneTreei ENSGT00390000011515.
    HOGENOMi HOG000000855.
    KOi K14838.
    OMAi KGFHERE.
    OrthoDBi EOG747PVB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33134-MONOMER.

    Miscellaneous databases

    NextBioi 979795.

    Gene expression databases

    Genevestigatori P53927.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV."
      Capozzo C., Sartorello F., Dal Pero F., D'Angelo M., Vezzi A., Campanaro S., Valle G.
      Yeast 16:1089-1097(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    5. "Composition and functional characterization of yeast 66S ribosome assembly intermediates."
      Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr.
      Mol. Cell 8:505-515(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PRE-66S RIBOSOMAL PARTICLE, FUNCTION, INTERACTION WITH NOP7, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis."
      Oeffinger M., Tollervey D.
      EMBO J. 22:6573-6583(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, RNA-BINDING, DISRUPTION PHENOTYPE.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation."
      Horsey E.W., Jakovljevic J., Miles T.D., Harnpicharnchai P., Woolford J.L. Jr.
      RNA 10:813-827(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RRP1, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiNOP15_YEAST
    AccessioniPrimary (citable) accession number: P53927
    Secondary accession number(s): D6W171
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3