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Protein

Ribosome biogenesis protein 15

Gene

NOP15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.2 Publications

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. actomyosin contractile ring assembly Source: SGD
  2. ribosomal large subunit biogenesis Source: SGD
  3. rRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33134-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein 15
Alternative name(s):
Nucleolar protein 15
Gene namesi
Name:NOP15
Ordered Locus Names:YNL110C
ORF Names:N1954
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL110c.
SGDiS000005054. NOP15.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleolus Source: SGD
  3. nucleus Source: SGD
  4. preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Abrupt growth arrest prior to substantial depletion of ribosomal subunits. Fails to synthesize the 25S and 5.8S rRNA components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA is strongly inhibited. Arrests at cytokinesis and fails to assemble a contractile actin ring at the bud neck.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ribosome biogenesis protein 15PRO_0000082035Add
BLAST

Proteomic databases

MaxQBiP53927.
PaxDbiP53927.
PeptideAtlasiP53927.

Expressioni

Gene expression databases

GenevestigatoriP53927.

Interactioni

Subunit structurei

Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIC1P387794EBI-28853,EBI-24538

Protein-protein interaction databases

BioGridi35715. 222 interactions.
DIPiDIP-4741N.
IntActiP53927. 51 interactions.
MINTiMINT-551800.
STRINGi4932.YNL110C.

Structurei

3D structure databases

ProteinModelPortaliP53927.
SMRiP53927. Positions 59-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 16979RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG244773.
GeneTreeiENSGT00390000011515.
HOGENOMiHOG000000855.
InParanoidiP53927.
KOiK14838.
OMAiKGFHERE.
OrthoDBiEOG747PVB.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53927-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI
60 70 80 90 100
EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH
110 120 130 140 150
GFHEKELSKY FAQFGDLKEV RLARNKKTGN SRHYGFLEFV NKEDAMIAQE
160 170 180 190 200
SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK KRVLVEKGIT KPVKQLKDNM
210 220
KQKHEERIKK LAKSGIEFKW
Length:220
Mass (Da):25,447
Last modified:October 1, 1996 - v1
Checksum:iEC86E3C77C863292
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA. Translation: CAA93397.1.
Z71386 Genomic DNA. Translation: CAA95989.1.
AY693098 Genomic DNA. Translation: AAT93117.1.
BK006947 Genomic DNA. Translation: DAA10437.1.
PIRiS63051.
RefSeqiNP_014289.1. NM_001182948.1.

Genome annotation databases

EnsemblFungiiYNL110C; YNL110C; YNL110C.
GeneIDi855613.
KEGGisce:YNL110C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA. Translation: CAA93397.1.
Z71386 Genomic DNA. Translation: CAA95989.1.
AY693098 Genomic DNA. Translation: AAT93117.1.
BK006947 Genomic DNA. Translation: DAA10437.1.
PIRiS63051.
RefSeqiNP_014289.1. NM_001182948.1.

3D structure databases

ProteinModelPortaliP53927.
SMRiP53927. Positions 59-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35715. 222 interactions.
DIPiDIP-4741N.
IntActiP53927. 51 interactions.
MINTiMINT-551800.
STRINGi4932.YNL110C.

Proteomic databases

MaxQBiP53927.
PaxDbiP53927.
PeptideAtlasiP53927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL110C; YNL110C; YNL110C.
GeneIDi855613.
KEGGisce:YNL110C.

Organism-specific databases

CYGDiYNL110c.
SGDiS000005054. NOP15.

Phylogenomic databases

eggNOGiNOG244773.
GeneTreeiENSGT00390000011515.
HOGENOMiHOG000000855.
InParanoidiP53927.
KOiK14838.
OMAiKGFHERE.
OrthoDBiEOG747PVB.

Enzyme and pathway databases

BioCyciYEAST:G3O-33134-MONOMER.

Miscellaneous databases

NextBioi979795.

Gene expression databases

GenevestigatoriP53927.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV."
    Capozzo C., Sartorello F., Dal Pero F., D'Angelo M., Vezzi A., Campanaro S., Valle G.
    Yeast 16:1089-1097(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  5. "Composition and functional characterization of yeast 66S ribosome assembly intermediates."
    Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr.
    Mol. Cell 8:505-515(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PRE-66S RIBOSOMAL PARTICLE, FUNCTION, INTERACTION WITH NOP7, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis."
    Oeffinger M., Tollervey D.
    EMBO J. 22:6573-6583(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, RNA-BINDING, DISRUPTION PHENOTYPE.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation."
    Horsey E.W., Jakovljevic J., Miles T.D., Harnpicharnchai P., Woolford J.L. Jr.
    RNA 10:813-827(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RRP1, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNOP15_YEAST
AccessioniPrimary (citable) accession number: P53927
Secondary accession number(s): D6W171
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.