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P53927 (NOP15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome biogenesis protein 15
Alternative name(s):
Nucleolar protein 15
Gene names
Name:NOP15
Ordered Locus Names:YNL110C
ORF Names:N1954
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs. Ref.5 Ref.6

Subunit structure

Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1. Ref.5 Ref.6 Ref.8

Subcellular location

Cytoplasm. Nucleusnucleolus Ref.4 Ref.5.

Disruption phenotype

Essential gene. Abrupt growth arrest prior to substantial depletion of ribosomal subunits. Fails to synthesize the 25S and 5.8S rRNA components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA is strongly inhibited. Arrests at cytokinesis and fails to assemble a contractile actin ring at the bud neck. Ref.4 Ref.6

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CIC1P387794EBI-28853,EBI-24538

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Ribosome biogenesis protein 15
PRO_0000082035

Regions

Domain91 – 16979RRM

Sequences

Sequence LengthMass (Da)Tools
P53927 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: EC86E3C77C863292

FASTA22025,447
        10         20         30         40         50         60 
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI EGLAASDDEQ 

        70         80         90        100        110        120 
SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH GFHEKELSKY FAQFGDLKEV 

       130        140        150        160        170        180 
RLARNKKTGN SRHYGFLEFV NKEDAMIAQE SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK 

       190        200        210        220 
KRVLVEKGIT KPVKQLKDNM KQKHEERIKK LAKSGIEFKW 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV."
Capozzo C., Sartorello F., Dal Pero F., D'Angelo M., Vezzi A., Campanaro S., Valle G.
Yeast 16:1089-1097(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[5]"Composition and functional characterization of yeast 66S ribosome assembly intermediates."
Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr.
Mol. Cell 8:505-515(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PRE-66S RIBOSOMAL PARTICLE, FUNCTION, INTERACTION WITH NOP7, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis."
Oeffinger M., Tollervey D.
EMBO J. 22:6573-6583(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, RNA-BINDING, DISRUPTION PHENOTYPE.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation."
Horsey E.W., Jakovljevic J., Miles T.D., Harnpicharnchai P., Woolford J.L. Jr.
RNA 10:813-827(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RRP1, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z69382 Genomic DNA. Translation: CAA93397.1.
Z71386 Genomic DNA. Translation: CAA95989.1.
AY693098 Genomic DNA. Translation: AAT93117.1.
BK006947 Genomic DNA. Translation: DAA10437.1.
PIRS63051.
RefSeqNP_014289.1. NM_001182948.1.

3D structure databases

ProteinModelPortalP53927.
SMRP53927. Positions 59-164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35715. 222 interactions.
DIPDIP-4741N.
IntActP53927. 51 interactions.
MINTMINT-551800.
STRING4932.YNL110C.

Proteomic databases

MaxQBP53927.
PaxDbP53927.
PeptideAtlasP53927.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL110C; YNL110C; YNL110C.
GeneID855613.
KEGGsce:YNL110C.

Organism-specific databases

CYGDYNL110c.
SGDS000005054. NOP15.

Phylogenomic databases

eggNOGNOG244773.
GeneTreeENSGT00390000011515.
HOGENOMHOG000000855.
KOK14838.
OMAKGFHERE.
OrthoDBEOG747PVB.

Enzyme and pathway databases

BioCycYEAST:G3O-33134-MONOMER.

Gene expression databases

GenevestigatorP53927.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979795.

Entry information

Entry nameNOP15_YEAST
AccessionPrimary (citable) accession number: P53927
Secondary accession number(s): D6W171
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families