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P53920 (NM111_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pro-apoptotic serine protease NMA111

EC=3.4.21.-
Alternative name(s):
111 kDa nuclear mediator of apoptosis
Gene names
Name:NMA111
Synonyms:YNM3
Ordered Locus Names:YNL123W
ORF Names:N1897
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length997 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nuclear serine protease which mediates apoptosis through proteolysis of the apoptotic inhibitor BIR1. Ref.8 Ref.9 Ref.10

Subunit structure

Interacts with BIR1. Ref.10

Subcellular location

Nucleus Ref.6 Ref.8.

Miscellaneous

Present with 3150 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase S1B family.

Contains 2 PDZ (DHR) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 997997Pro-apoptotic serine protease NMA111
PRO_0000203429

Regions

Domain300 – 37879PDZ 1
Domain779 – 85476PDZ 2
Region83 – 273191Serine protease

Sites

Active site1211Charge relay system Potential
Active site1521Charge relay system Potential
Active site2351Charge relay system Potential

Natural variations

Natural variant1621N → S in strain: YJM269, YJM270 and YJM1129. Ref.1
Natural variant2411V → L in strain: YJM269 and YJM270. Ref.1
Natural variant3311E → G in strain: YJM326. Ref.1
Natural variant3431T → S in strain: YJM269 and YJM270. Ref.1
Natural variant3861T → I in strain: YJM269, YJM270 and YJM1129. Ref.1
Natural variant4571D → G in strain: YJM269 and YJM270. Ref.1
Natural variant5301S → P in strain: YJM627. Ref.1
Natural variant5801K → N in strain: YJM627. Ref.1
Natural variant6211A → T in strain: YJM269 and YJM270. Ref.1
Natural variant9421P → F in strain: YJM627. Ref.1

Experimental info

Mutagenesis2351S → C: Impairs BIR1 degradation and death-promoting activity. Ref.8 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P53920 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A26005C1DDDB932C

FASTA997110,881
        10         20         30         40         50         60 
MTISLSNIKK RDHSKISDGT SGESSLVKRK QLESATGDQE EEYTDHEIII EPLHFANNNN 

        70         80         90        100        110        120 
TVLTDSENYL RWQNTISNVV KSVVSIHFSQ VAPFDCDSAL VSEATGFVVD AKLGIILTNR 

       130        140        150        160        170        180 
HVVGPGPFVG YVVFDNHEEC DVIPIYRDPV HDFGFLKFDP KNIKYSKIKA LTLKPSLAKV 

       190        200        210        220        230        240 
GSEIRVVGND AGEKLSILAG FISRIDRNAP EYGELTYNDF NTEYIQAAAS ASGGSSGSPV 

       250        260        270        280        290        300 
VNIDGYAVAL QAGGSTEAST DFFLPLDRIL RALICIQTNK PITRGTIQVQ WLLKPYDECR 

       310        320        330        340        350        360 
RLGLTSERES EARAKFPENI GLLVAETVLR EGPGYDKIKE GDTLISINGE TISSFMQVDK 

       370        380        390        400        410        420 
IQDENVGKEI QLVIQRGGVE CTVTCTVGDL HAITPHRYVE VCGATFHELS YQMARFYALP 

       430        440        450        460        470        480 
VRGVFLSSAS GSFNFDSKER VGWIVDSIDN KETPDLDTFI EIMKTIPDRK RVTVRYHHLT 

       490        500        510        520        530        540 
DQHSPLVTSI YIDRHWCNEF RVYTRNDTTG IWDYKNVADP LPADALKPRS AKIIPIPVNN 

       550        560        570        580        590        600 
EKVAKLSSSL CTVATMAAVP LDSLSADILK TSGLIIDAEK GYVLVSRRVV PHDCLDTFVT 

       610        620        630        640        650        660 
IADSLVVPAT VEFLHPTHNF AIVKYDPELV KAPLITPKLS TTRMKRGDKL QFIGFTQNDR 

       670        680        690        700        710        720 
IVTSETTVTD ISSVSIPSNL IPRYRATNLE AISIDCNVST RCNSGILTDN DGTVRGLWLP 

       730        740        750        760        770        780 
FLGERLENKE KVYLMGLDIM DCREVIDILK NGGKPRVSIV DAGFGSISVL QARIRGVPEE 

       790        800        810        820        830        840 
WIMRMEHESN NRLQFITVSR VSYTEDKIHL ETGDVILSVN GKLVTEMNDL NGVVSSADGI 

       850        860        870        880        890        900 
LPSAMLDFKV VRDGNIVDLK IKTVEVQETD RFVIFAGSIL QKPHHAVLQA MVDVPKGVYC 

       910        920        930        940        950        960 
TFRGESSPAL QYGISATNFI THVNEIETPD LDTFLKVVKT IPDNSYCKMR LMTFDNVPFA 

       970        980        990 
ISLKTNYHYF PTAELKRDNI THKWIEKEFT GNSQSEK 

« Hide

References

« Hide 'large scale' references
[1]"Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-162; LEU-241; GLY-331; SER-343; ILE-386; GLY-457; PRO-530; ASN-580; THR-621 AND PHE-942.
Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
[2]"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels."
Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M.
Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis."
Fahrenkrog B., Sauder U., Aebi U.
J. Cell Sci. 117:115-126(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF SER-235.
[9]"Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast."
Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S.
Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2."
Walter D., Wissing S., Madeo F., Fahrenkrog B.
J. Cell Sci. 119:1843-1851(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF SER-235, INTERACTION WITH BIR1.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF125216 Genomic DNA. Translation: ABN58534.1.
EF125217 Genomic DNA. Translation: ABN58544.1.
EF125218 Genomic DNA. Translation: ABN58553.1.
EF125219 Genomic DNA. Translation: ABN58562.1.
EF125220 Genomic DNA. Translation: ABN58571.1.
EF125221 Genomic DNA. Translation: ABN58580.1.
EF125222 Genomic DNA. Translation: ABN58589.1.
EF125223 Genomic DNA. Translation: ABN58598.1.
EF125224 Genomic DNA. Translation: ABN58607.1.
EF125225 Genomic DNA. Translation: ABN58616.1.
EF125226 Genomic DNA. Translation: ABN58625.1.
EF125228 Genomic DNA. Translation: ABN58643.1.
Z69382 Genomic DNA. Translation: CAA93384.1.
Z71399 Genomic DNA. Translation: CAA96004.1.
BK006947 Genomic DNA. Translation: DAA10426.1.
PIRS63064.
RefSeqNP_014276.1. NM_001182961.1.

3D structure databases

ProteinModelPortalP53920.
SMRP53920. Positions 74-388, 805-880.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35704. 15 interactions.
MINTMINT-4499860.
STRING4932.YNL123W.

Protein family/group databases

MEROPSS01.434.

Proteomic databases

MaxQBP53920.
PaxDbP53920.
PeptideAtlasP53920.
PRIDEP53920.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL123W; YNL123W; YNL123W.
GeneID855600.
KEGGsce:YNL123W.

Organism-specific databases

CYGDYNL123w.
SGDS000005067. NMA111.

Phylogenomic databases

eggNOGCOG0265.
OMATCSFDTD.
OrthoDBEOG7XH6ZJ.

Enzyme and pathway databases

BioCycYEAST:G3O-33144-MONOMER.

Gene expression databases

GenevestigatorP53920.

Family and domain databases

Gene3D2.30.42.10. 2 hits.
InterProIPR001478. PDZ.
IPR025926. PDZ-like_dom.
IPR001940. Peptidase_S1C.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
PF12812. PDZ_1. 2 hits.
[Graphical view]
PRINTSPR00834. PROTEASES2C.
SMARTSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMSSF50156. SSF50156. 3 hits.
SSF50494. SSF50494. 2 hits.
ProtoNetSearch...

Other

NextBio979762.

Entry information

Entry nameNM111_YEAST
AccessionPrimary (citable) accession number: P53920
Secondary accession number(s): B0KZR3 expand/collapse secondary AC list , B0KZU0, B0KZX6, B0L003, B0L021, D6W160
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries