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P53920

- NM111_YEAST

UniProt

P53920 - NM111_YEAST

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Protein
Pro-apoptotic serine protease NMA111
Gene
NMA111, YNM3, YNL123W, N1897
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Nuclear serine protease which mediates apoptosis through proteolysis of the apoptotic inhibitor BIR1.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei121 – 1211Charge relay system Reviewed prediction
Active sitei152 – 1521Charge relay system Reviewed prediction
Active sitei235 – 2351Charge relay system Reviewed prediction

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type peptidase activity Source: SGD

GO - Biological processi

  1. apoptotic process Source: SGD
  2. cellular lipid metabolic process Source: SGD
  3. cellular response to heat Source: SGD
  4. protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33144-MONOMER.

Protein family/group databases

MEROPSiS01.434.

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-apoptotic serine protease NMA111 (EC:3.4.21.-)
Alternative name(s):
111 kDa nuclear mediator of apoptosis
Gene namesi
Name:NMA111
Synonyms:YNM3
Ordered Locus Names:YNL123W
ORF Names:N1897
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL123w.
SGDiS000005067. NMA111.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi235 – 2351S → C: Impairs BIR1 degradation and death-promoting activity. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 997997Pro-apoptotic serine protease NMA111
PRO_0000203429Add
BLAST

Proteomic databases

MaxQBiP53920.
PaxDbiP53920.
PeptideAtlasiP53920.
PRIDEiP53920.

Expressioni

Gene expression databases

GenevestigatoriP53920.

Interactioni

Subunit structurei

Interacts with BIR1.1 Publication

Protein-protein interaction databases

BioGridi35704. 15 interactions.
MINTiMINT-4499860.
STRINGi4932.YNL123W.

Structurei

3D structure databases

ProteinModelPortaliP53920.
SMRiP53920. Positions 74-388, 805-880.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini300 – 37879PDZ 1
Add
BLAST
Domaini779 – 85476PDZ 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 273191Serine protease
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1B family.
Contains 2 PDZ (DHR) domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0265.
OMAiTCSFDTD.
OrthoDBiEOG7XH6ZJ.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR001478. PDZ.
IPR025926. PDZ-like_dom.
IPR001940. Peptidase_S1C.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
PF12812. PDZ_1. 2 hits.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 3 hits.
SSF50494. SSF50494. 2 hits.

Sequencei

Sequence statusi: Complete.

P53920-1 [UniParc]FASTAAdd to Basket

« Hide

MTISLSNIKK RDHSKISDGT SGESSLVKRK QLESATGDQE EEYTDHEIII    50
EPLHFANNNN TVLTDSENYL RWQNTISNVV KSVVSIHFSQ VAPFDCDSAL 100
VSEATGFVVD AKLGIILTNR HVVGPGPFVG YVVFDNHEEC DVIPIYRDPV 150
HDFGFLKFDP KNIKYSKIKA LTLKPSLAKV GSEIRVVGND AGEKLSILAG 200
FISRIDRNAP EYGELTYNDF NTEYIQAAAS ASGGSSGSPV VNIDGYAVAL 250
QAGGSTEAST DFFLPLDRIL RALICIQTNK PITRGTIQVQ WLLKPYDECR 300
RLGLTSERES EARAKFPENI GLLVAETVLR EGPGYDKIKE GDTLISINGE 350
TISSFMQVDK IQDENVGKEI QLVIQRGGVE CTVTCTVGDL HAITPHRYVE 400
VCGATFHELS YQMARFYALP VRGVFLSSAS GSFNFDSKER VGWIVDSIDN 450
KETPDLDTFI EIMKTIPDRK RVTVRYHHLT DQHSPLVTSI YIDRHWCNEF 500
RVYTRNDTTG IWDYKNVADP LPADALKPRS AKIIPIPVNN EKVAKLSSSL 550
CTVATMAAVP LDSLSADILK TSGLIIDAEK GYVLVSRRVV PHDCLDTFVT 600
IADSLVVPAT VEFLHPTHNF AIVKYDPELV KAPLITPKLS TTRMKRGDKL 650
QFIGFTQNDR IVTSETTVTD ISSVSIPSNL IPRYRATNLE AISIDCNVST 700
RCNSGILTDN DGTVRGLWLP FLGERLENKE KVYLMGLDIM DCREVIDILK 750
NGGKPRVSIV DAGFGSISVL QARIRGVPEE WIMRMEHESN NRLQFITVSR 800
VSYTEDKIHL ETGDVILSVN GKLVTEMNDL NGVVSSADGI LPSAMLDFKV 850
VRDGNIVDLK IKTVEVQETD RFVIFAGSIL QKPHHAVLQA MVDVPKGVYC 900
TFRGESSPAL QYGISATNFI THVNEIETPD LDTFLKVVKT IPDNSYCKMR 950
LMTFDNVPFA ISLKTNYHYF PTAELKRDNI THKWIEKEFT GNSQSEK 997
Length:997
Mass (Da):110,881
Last modified:October 1, 1996 - v1
Checksum:iA26005C1DDDB932C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti162 – 1621N → S in strain: YJM269, YJM270 and YJM1129. 1 Publication
Natural varianti241 – 2411V → L in strain: YJM269 and YJM270. 1 Publication
Natural varianti331 – 3311E → G in strain: YJM326. 1 Publication
Natural varianti343 – 3431T → S in strain: YJM269 and YJM270. 1 Publication
Natural varianti386 – 3861T → I in strain: YJM269, YJM270 and YJM1129. 1 Publication
Natural varianti457 – 4571D → G in strain: YJM269 and YJM270. 1 Publication
Natural varianti530 – 5301S → P in strain: YJM627. 1 Publication
Natural varianti580 – 5801K → N in strain: YJM627. 1 Publication
Natural varianti621 – 6211A → T in strain: YJM269 and YJM270. 1 Publication
Natural varianti942 – 9421P → F in strain: YJM627. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF125216 Genomic DNA. Translation: ABN58534.1.
EF125217 Genomic DNA. Translation: ABN58544.1.
EF125218 Genomic DNA. Translation: ABN58553.1.
EF125219 Genomic DNA. Translation: ABN58562.1.
EF125220 Genomic DNA. Translation: ABN58571.1.
EF125221 Genomic DNA. Translation: ABN58580.1.
EF125222 Genomic DNA. Translation: ABN58589.1.
EF125223 Genomic DNA. Translation: ABN58598.1.
EF125224 Genomic DNA. Translation: ABN58607.1.
EF125225 Genomic DNA. Translation: ABN58616.1.
EF125226 Genomic DNA. Translation: ABN58625.1.
EF125228 Genomic DNA. Translation: ABN58643.1.
Z69382 Genomic DNA. Translation: CAA93384.1.
Z71399 Genomic DNA. Translation: CAA96004.1.
BK006947 Genomic DNA. Translation: DAA10426.1.
PIRiS63064.
RefSeqiNP_014276.1. NM_001182961.1.

Genome annotation databases

EnsemblFungiiYNL123W; YNL123W; YNL123W.
GeneIDi855600.
KEGGisce:YNL123W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF125216 Genomic DNA. Translation: ABN58534.1 .
EF125217 Genomic DNA. Translation: ABN58544.1 .
EF125218 Genomic DNA. Translation: ABN58553.1 .
EF125219 Genomic DNA. Translation: ABN58562.1 .
EF125220 Genomic DNA. Translation: ABN58571.1 .
EF125221 Genomic DNA. Translation: ABN58580.1 .
EF125222 Genomic DNA. Translation: ABN58589.1 .
EF125223 Genomic DNA. Translation: ABN58598.1 .
EF125224 Genomic DNA. Translation: ABN58607.1 .
EF125225 Genomic DNA. Translation: ABN58616.1 .
EF125226 Genomic DNA. Translation: ABN58625.1 .
EF125228 Genomic DNA. Translation: ABN58643.1 .
Z69382 Genomic DNA. Translation: CAA93384.1 .
Z71399 Genomic DNA. Translation: CAA96004.1 .
BK006947 Genomic DNA. Translation: DAA10426.1 .
PIRi S63064.
RefSeqi NP_014276.1. NM_001182961.1.

3D structure databases

ProteinModelPortali P53920.
SMRi P53920. Positions 74-388, 805-880.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35704. 15 interactions.
MINTi MINT-4499860.
STRINGi 4932.YNL123W.

Protein family/group databases

MEROPSi S01.434.

Proteomic databases

MaxQBi P53920.
PaxDbi P53920.
PeptideAtlasi P53920.
PRIDEi P53920.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL123W ; YNL123W ; YNL123W .
GeneIDi 855600.
KEGGi sce:YNL123W.

Organism-specific databases

CYGDi YNL123w.
SGDi S000005067. NMA111.

Phylogenomic databases

eggNOGi COG0265.
OMAi TCSFDTD.
OrthoDBi EOG7XH6ZJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-33144-MONOMER.

Miscellaneous databases

NextBioi 979762.

Gene expression databases

Genevestigatori P53920.

Family and domain databases

Gene3Di 2.30.42.10. 2 hits.
InterProi IPR001478. PDZ.
IPR025926. PDZ-like_dom.
IPR001940. Peptidase_S1C.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00595. PDZ. 1 hit.
PF12812. PDZ_1. 2 hits.
[Graphical view ]
PRINTSi PR00834. PROTEASES2C.
SMARTi SM00228. PDZ. 2 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 3 hits.
SSF50494. SSF50494. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
    Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
    Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-162; LEU-241; GLY-331; SER-343; ILE-386; GLY-457; PRO-530; ASN-580; THR-621 AND PHE-942.
    Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
  2. "The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
    de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
    Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels."
    Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M.
    Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis."
    Fahrenkrog B., Sauder U., Aebi U.
    J. Cell Sci. 117:115-126(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF SER-235.
  9. "Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast."
    Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S.
    Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2."
    Walter D., Wissing S., Madeo F., Fahrenkrog B.
    J. Cell Sci. 119:1843-1851(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-235, INTERACTION WITH BIR1.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNM111_YEAST
AccessioniPrimary (citable) accession number: P53920
Secondary accession number(s): B0KZR3
, B0KZU0, B0KZX6, B0L003, B0L021, D6W160
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3150 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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