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P53920

- NM111_YEAST

UniProt

P53920 - NM111_YEAST

Protein

Pro-apoptotic serine protease NMA111

Gene

NMA111

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Nuclear serine protease which mediates apoptosis through proteolysis of the apoptotic inhibitor BIR1.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei121 – 1211Charge relay systemSequence Analysis
    Active sitei152 – 1521Charge relay systemSequence Analysis
    Active sitei235 – 2351Charge relay systemSequence Analysis

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro
    2. serine-type peptidase activity Source: SGD

    GO - Biological processi

    1. apoptotic process Source: SGD
    2. cellular lipid metabolic process Source: SGD
    3. cellular response to heat Source: SGD
    4. protein catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33144-MONOMER.

    Protein family/group databases

    MEROPSiS01.434.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pro-apoptotic serine protease NMA111 (EC:3.4.21.-)
    Alternative name(s):
    111 kDa nuclear mediator of apoptosis
    Gene namesi
    Name:NMA111
    Synonyms:YNM3
    Ordered Locus Names:YNL123W
    ORF Names:N1897
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL123w.
    SGDiS000005067. NMA111.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi235 – 2351S → C: Impairs BIR1 degradation and death-promoting activity. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 997997Pro-apoptotic serine protease NMA111PRO_0000203429Add
    BLAST

    Proteomic databases

    MaxQBiP53920.
    PaxDbiP53920.
    PeptideAtlasiP53920.
    PRIDEiP53920.

    Expressioni

    Gene expression databases

    GenevestigatoriP53920.

    Interactioni

    Subunit structurei

    Interacts with BIR1.1 Publication

    Protein-protein interaction databases

    BioGridi35704. 15 interactions.
    MINTiMINT-4499860.
    STRINGi4932.YNL123W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53920.
    SMRiP53920. Positions 74-388, 805-880.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini300 – 37879PDZ 1Add
    BLAST
    Domaini779 – 85476PDZ 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni83 – 273191Serine proteaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1B family.Curated
    Contains 2 PDZ (DHR) domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0265.
    OMAiTCSFDTD.
    OrthoDBiEOG7XH6ZJ.

    Family and domain databases

    Gene3Di2.30.42.10. 2 hits.
    InterProiIPR001478. PDZ.
    IPR025926. PDZ-like_dom.
    IPR001940. Peptidase_S1C.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00595. PDZ. 1 hit.
    PF12812. PDZ_1. 2 hits.
    [Graphical view]
    PRINTSiPR00834. PROTEASES2C.
    SMARTiSM00228. PDZ. 2 hits.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 3 hits.
    SSF50494. SSF50494. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P53920-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTISLSNIKK RDHSKISDGT SGESSLVKRK QLESATGDQE EEYTDHEIII    50
    EPLHFANNNN TVLTDSENYL RWQNTISNVV KSVVSIHFSQ VAPFDCDSAL 100
    VSEATGFVVD AKLGIILTNR HVVGPGPFVG YVVFDNHEEC DVIPIYRDPV 150
    HDFGFLKFDP KNIKYSKIKA LTLKPSLAKV GSEIRVVGND AGEKLSILAG 200
    FISRIDRNAP EYGELTYNDF NTEYIQAAAS ASGGSSGSPV VNIDGYAVAL 250
    QAGGSTEAST DFFLPLDRIL RALICIQTNK PITRGTIQVQ WLLKPYDECR 300
    RLGLTSERES EARAKFPENI GLLVAETVLR EGPGYDKIKE GDTLISINGE 350
    TISSFMQVDK IQDENVGKEI QLVIQRGGVE CTVTCTVGDL HAITPHRYVE 400
    VCGATFHELS YQMARFYALP VRGVFLSSAS GSFNFDSKER VGWIVDSIDN 450
    KETPDLDTFI EIMKTIPDRK RVTVRYHHLT DQHSPLVTSI YIDRHWCNEF 500
    RVYTRNDTTG IWDYKNVADP LPADALKPRS AKIIPIPVNN EKVAKLSSSL 550
    CTVATMAAVP LDSLSADILK TSGLIIDAEK GYVLVSRRVV PHDCLDTFVT 600
    IADSLVVPAT VEFLHPTHNF AIVKYDPELV KAPLITPKLS TTRMKRGDKL 650
    QFIGFTQNDR IVTSETTVTD ISSVSIPSNL IPRYRATNLE AISIDCNVST 700
    RCNSGILTDN DGTVRGLWLP FLGERLENKE KVYLMGLDIM DCREVIDILK 750
    NGGKPRVSIV DAGFGSISVL QARIRGVPEE WIMRMEHESN NRLQFITVSR 800
    VSYTEDKIHL ETGDVILSVN GKLVTEMNDL NGVVSSADGI LPSAMLDFKV 850
    VRDGNIVDLK IKTVEVQETD RFVIFAGSIL QKPHHAVLQA MVDVPKGVYC 900
    TFRGESSPAL QYGISATNFI THVNEIETPD LDTFLKVVKT IPDNSYCKMR 950
    LMTFDNVPFA ISLKTNYHYF PTAELKRDNI THKWIEKEFT GNSQSEK 997
    Length:997
    Mass (Da):110,881
    Last modified:October 1, 1996 - v1
    Checksum:iA26005C1DDDB932C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti162 – 1621N → S in strain: YJM269, YJM270 and YJM1129. 1 Publication
    Natural varianti241 – 2411V → L in strain: YJM269 and YJM270. 1 Publication
    Natural varianti331 – 3311E → G in strain: YJM326. 1 Publication
    Natural varianti343 – 3431T → S in strain: YJM269 and YJM270. 1 Publication
    Natural varianti386 – 3861T → I in strain: YJM269, YJM270 and YJM1129. 1 Publication
    Natural varianti457 – 4571D → G in strain: YJM269 and YJM270. 1 Publication
    Natural varianti530 – 5301S → P in strain: YJM627. 1 Publication
    Natural varianti580 – 5801K → N in strain: YJM627. 1 Publication
    Natural varianti621 – 6211A → T in strain: YJM269 and YJM270. 1 Publication
    Natural varianti942 – 9421P → F in strain: YJM627. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF125216 Genomic DNA. Translation: ABN58534.1.
    EF125217 Genomic DNA. Translation: ABN58544.1.
    EF125218 Genomic DNA. Translation: ABN58553.1.
    EF125219 Genomic DNA. Translation: ABN58562.1.
    EF125220 Genomic DNA. Translation: ABN58571.1.
    EF125221 Genomic DNA. Translation: ABN58580.1.
    EF125222 Genomic DNA. Translation: ABN58589.1.
    EF125223 Genomic DNA. Translation: ABN58598.1.
    EF125224 Genomic DNA. Translation: ABN58607.1.
    EF125225 Genomic DNA. Translation: ABN58616.1.
    EF125226 Genomic DNA. Translation: ABN58625.1.
    EF125228 Genomic DNA. Translation: ABN58643.1.
    Z69382 Genomic DNA. Translation: CAA93384.1.
    Z71399 Genomic DNA. Translation: CAA96004.1.
    BK006947 Genomic DNA. Translation: DAA10426.1.
    PIRiS63064.
    RefSeqiNP_014276.1. NM_001182961.1.

    Genome annotation databases

    EnsemblFungiiYNL123W; YNL123W; YNL123W.
    GeneIDi855600.
    KEGGisce:YNL123W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF125216 Genomic DNA. Translation: ABN58534.1 .
    EF125217 Genomic DNA. Translation: ABN58544.1 .
    EF125218 Genomic DNA. Translation: ABN58553.1 .
    EF125219 Genomic DNA. Translation: ABN58562.1 .
    EF125220 Genomic DNA. Translation: ABN58571.1 .
    EF125221 Genomic DNA. Translation: ABN58580.1 .
    EF125222 Genomic DNA. Translation: ABN58589.1 .
    EF125223 Genomic DNA. Translation: ABN58598.1 .
    EF125224 Genomic DNA. Translation: ABN58607.1 .
    EF125225 Genomic DNA. Translation: ABN58616.1 .
    EF125226 Genomic DNA. Translation: ABN58625.1 .
    EF125228 Genomic DNA. Translation: ABN58643.1 .
    Z69382 Genomic DNA. Translation: CAA93384.1 .
    Z71399 Genomic DNA. Translation: CAA96004.1 .
    BK006947 Genomic DNA. Translation: DAA10426.1 .
    PIRi S63064.
    RefSeqi NP_014276.1. NM_001182961.1.

    3D structure databases

    ProteinModelPortali P53920.
    SMRi P53920. Positions 74-388, 805-880.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35704. 15 interactions.
    MINTi MINT-4499860.
    STRINGi 4932.YNL123W.

    Protein family/group databases

    MEROPSi S01.434.

    Proteomic databases

    MaxQBi P53920.
    PaxDbi P53920.
    PeptideAtlasi P53920.
    PRIDEi P53920.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL123W ; YNL123W ; YNL123W .
    GeneIDi 855600.
    KEGGi sce:YNL123W.

    Organism-specific databases

    CYGDi YNL123w.
    SGDi S000005067. NMA111.

    Phylogenomic databases

    eggNOGi COG0265.
    OMAi TCSFDTD.
    OrthoDBi EOG7XH6ZJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33144-MONOMER.

    Miscellaneous databases

    NextBioi 979762.

    Gene expression databases

    Genevestigatori P53920.

    Family and domain databases

    Gene3Di 2.30.42.10. 2 hits.
    InterProi IPR001478. PDZ.
    IPR025926. PDZ-like_dom.
    IPR001940. Peptidase_S1C.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00595. PDZ. 1 hit.
    PF12812. PDZ_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00834. PROTEASES2C.
    SMARTi SM00228. PDZ. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 3 hits.
    SSF50494. SSF50494. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
      Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
      Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-162; LEU-241; GLY-331; SER-343; ILE-386; GLY-457; PRO-530; ASN-580; THR-621 AND PHE-942.
      Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
    2. "The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
      de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
      Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels."
      Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M.
      Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis."
      Fahrenkrog B., Sauder U., Aebi U.
      J. Cell Sci. 117:115-126(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF SER-235.
    9. "Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast."
      Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S.
      Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2."
      Walter D., Wissing S., Madeo F., Fahrenkrog B.
      J. Cell Sci. 119:1843-1851(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-235, INTERACTION WITH BIR1.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNM111_YEAST
    AccessioniPrimary (citable) accession number: P53920
    Secondary accession number(s): B0KZR3
    , B0KZU0, B0KZX6, B0L003, B0L021, D6W160
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3150 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3