P53920 (NM111_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pro-apoptotic serine protease NMA111
EC=3.4.21.-

Alternative name(s):
111 kDa nuclear mediator of apoptosis

Gene names

Name:	NMA111
Synonyms:	YNM3
Ordered Locus Names:	YNL123W
ORF Names:	N1897

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	997 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nuclear serine protease which mediates apoptosis through proteolysis of the apoptotic inhibitor BIR1. Ref.8 Ref.9 Ref.10
Subunit structure	Interacts with BIR1. Ref.10
Subcellular location	Nucleus Ref.6 Ref.8.
Miscellaneous	Present with 3150 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase S1B family. Contains 2 PDZ (DHR) domains.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Nucleus
Domain	Repeat
Molecular function	Hydrolase Protease Serine protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from mutant phenotype Ref.8PubMed 16470384Ref.10. Source: SGD cellular lipid metabolic process Inferred from mutant phenotype PubMed 16470384. Source: SGD cellular response to heat Inferred from mutant phenotype PubMed 18946088. Source: SGD protein catabolic process Inferred from mutant phenotype PubMed 18946088. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.6Ref.8. Source: SGD
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type peptidase activity Inferred from direct assay PubMed 18946088. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 997	997	Pro-apoptotic serine protease NMA111		PRO_0000203429
Regions
	Domain	300 – 378	79	PDZ 1
	Domain	779 – 854	76	PDZ 2
	Region	83 – 273	191	Serine protease
Sites
	Active site	121	1	Charge relay system Potential
	Active site	152	1	Charge relay system Potential
	Active site	235	1	Charge relay system Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.11
	Modified residue	21	1	Phosphoserine Ref.11
	Modified residue	993	1	Phosphoserine Ref.11
Natural variations
	Natural variant	162	1	N → S in strain: YJM269, YJM270 and YJM1129. Ref.1
	Natural variant	241	1	V → L in strain: YJM269 and YJM270. Ref.1
	Natural variant	331	1	E → G in strain: YJM326. Ref.1
	Natural variant	343	1	T → S in strain: YJM269 and YJM270. Ref.1
	Natural variant	386	1	T → I in strain: YJM269, YJM270 and YJM1129. Ref.1
	Natural variant	457	1	D → G in strain: YJM269 and YJM270. Ref.1
	Natural variant	530	1	S → P in strain: YJM627. Ref.1
	Natural variant	580	1	K → N in strain: YJM627. Ref.1
	Natural variant	621	1	A → T in strain: YJM269 and YJM270. Ref.1
	Natural variant	942	1	P → F in strain: YJM627. Ref.1
Experimental info
	Mutagenesis	235	1	S → C: Impairs BIR1 degradation and death-promoting activity. Ref.8 Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P53920 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A26005C1DDDB932C	FASTA	997	110,881
	10 20 30 40 50 60 MTISLSNIKK RDHSKISDGT SGESSLVKRK QLESATGDQE EEYTDHEIII EPLHFANNNN 70 80 90 100 110 120 TVLTDSENYL RWQNTISNVV KSVVSIHFSQ VAPFDCDSAL VSEATGFVVD AKLGIILTNR 130 140 150 160 170 180 HVVGPGPFVG YVVFDNHEEC DVIPIYRDPV HDFGFLKFDP KNIKYSKIKA LTLKPSLAKV 190 200 210 220 230 240 GSEIRVVGND AGEKLSILAG FISRIDRNAP EYGELTYNDF NTEYIQAAAS ASGGSSGSPV 250 260 270 280 290 300 VNIDGYAVAL QAGGSTEAST DFFLPLDRIL RALICIQTNK PITRGTIQVQ WLLKPYDECR 310 320 330 340 350 360 RLGLTSERES EARAKFPENI GLLVAETVLR EGPGYDKIKE GDTLISINGE TISSFMQVDK 370 380 390 400 410 420 IQDENVGKEI QLVIQRGGVE CTVTCTVGDL HAITPHRYVE VCGATFHELS YQMARFYALP 430 440 450 460 470 480 VRGVFLSSAS GSFNFDSKER VGWIVDSIDN KETPDLDTFI EIMKTIPDRK RVTVRYHHLT 490 500 510 520 530 540 DQHSPLVTSI YIDRHWCNEF RVYTRNDTTG IWDYKNVADP LPADALKPRS AKIIPIPVNN 550 560 570 580 590 600 EKVAKLSSSL CTVATMAAVP LDSLSADILK TSGLIIDAEK GYVLVSRRVV PHDCLDTFVT 610 620 630 640 650 660 IADSLVVPAT VEFLHPTHNF AIVKYDPELV KAPLITPKLS TTRMKRGDKL QFIGFTQNDR 670 680 690 700 710 720 IVTSETTVTD ISSVSIPSNL IPRYRATNLE AISIDCNVST RCNSGILTDN DGTVRGLWLP 730 740 750 760 770 780 FLGERLENKE KVYLMGLDIM DCREVIDILK NGGKPRVSIV DAGFGSISVL QARIRGVPEE 790 800 810 820 830 840 WIMRMEHESN NRLQFITVSR VSYTEDKIHL ETGDVILSVN GKLVTEMNDL NGVVSSADGI 850 860 870 880 890 900 LPSAMLDFKV VRDGNIVDLK IKTVEVQETD RFVIFAGSIL QKPHHAVLQA MVDVPKGVYC 910 920 930 940 950 960 TFRGESSPAL QYGISATNFI THVNEIETPD LDTFLKVVKT IPDNSYCKMR LMTFDNVPFA 970 980 990 ISLKTNYHYF PTAELKRDNI THKWIEKEFT GNSQSEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast." Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M. Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-162; LEU-241; GLY-331; SER-343; ILE-386; GLY-457; PRO-530; ASN-580; THR-621 AND PHE-942. Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
[2]	"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames." de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G. Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels." Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M. Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis." Fahrenkrog B., Sauder U., Aebi U. J. Cell Sci. 117:115-126(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF SER-235.
[9]	"Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast." Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S. Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2." Walter D., Wissing S., Madeo F., Fahrenkrog B. J. Cell Sci. 119:1843-1851(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF SER-235, INTERACTION WITH BIR1.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-21 AND SER-993, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF125216 Genomic DNA. Translation: ABN58534.1. EF125217 Genomic DNA. Translation: ABN58544.1. EF125218 Genomic DNA. Translation: ABN58553.1. EF125219 Genomic DNA. Translation: ABN58562.1. EF125220 Genomic DNA. Translation: ABN58571.1. EF125221 Genomic DNA. Translation: ABN58580.1. EF125222 Genomic DNA. Translation: ABN58589.1. EF125223 Genomic DNA. Translation: ABN58598.1. EF125224 Genomic DNA. Translation: ABN58607.1. EF125225 Genomic DNA. Translation: ABN58616.1. EF125226 Genomic DNA. Translation: ABN58625.1. EF125228 Genomic DNA. Translation: ABN58643.1. Z69382 Genomic DNA. Translation: CAA93384.1. Z71399 Genomic DNA. Translation: CAA96004.1. BK006947 Genomic DNA. Translation: DAA10426.1.
PIR	S63064.
RefSeq	NP_014276.1. NM_001182961.1.
3D structure databases
ProteinModelPortal	P53920.
SMR	P53920. Positions 74-387, 810-851.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YNL123W.
Protein family/group databases
MEROPS	S01.434.
Proteomic databases
PaxDb	P53920.
PeptideAtlas	P53920.
PRIDE	P53920.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL123W; YNL123W; YNL123W.
GeneID	855600.
KEGG	sce:YNL123W.
Organism-specific databases
CYGD	YNL123w.
SGD	S000005067. NMA111.
Phylogenomic databases
eggNOG	COG0265.
GeneTree	ENSGT00510000046315.
OMA	VHDFGFL.
OrthoDB	EOG4ZW8K6.
Gene expression databases
Genevestigator	P53920.
GermOnline	YNL123W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001478. PDZ. IPR025926. PDZ-like_dom. IPR001940. Peptidase_S1C. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. PF12812. PDZ_1. 2 hits. [Graphical view]
PRINTS	PR00834. PROTEASES2C.
SMART	SM00228. PDZ. 2 hits. [Graphical view]
SUPFAM	SSF50156. PDZ. 2 hits. SSF50494. Pept_Ser_Cys. 2 hits.
PROSITE	PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979762.

Entry information

Entry name

NM111_YEAST

Accession

Primary (citable) accession number: P53920
Secondary accession number(s): B0KZR3 , B0KZU0, B0KZX6, B0L003, B0L021, D6W160

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program