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Protein

H/ACA ribonucleoprotein complex non-core subunit NAF1

Gene

NAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by GAR1 to yield mature H/ACA snoRNPs complex. Acts as a competitive binder for CBF5 probably required to prevent non-cognate RNAs from being loaded during transport of the particle by inducing a non-productive conformation of CBF5.5 Publications

Miscellaneous

Present with 1810 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • box H/ACA snoRNP assembly Source: SGD
  • pseudouridine synthesis Source: InterPro
  • rRNA processing Source: UniProtKB-KW

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33145-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex non-core subunit NAF1
Alternative name(s):
Nuclear assembly factor 1
Gene namesi
Name:NAF1
Ordered Locus Names:YNL124W
ORF Names:N1888
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL124W
SGDiS000005068 NAF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002034281 – 492H/ACA ribonucleoprotein complex non-core subunit NAF1Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei255PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53919
PaxDbiP53919
PRIDEiP53919

PTM databases

iPTMnetiP53919

Interactioni

Subunit structurei

During assembly of the complex, component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) which contains CBF5, NAF1, NHP2 and NOP10 proteins. Interacts with SHQ1. Interacts directly with CBF5. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (RPB1).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35703, 209 interactors
DIPiDIP-4345N
IntActiP53919, 257 interactors
MINTiP53919
STRINGi4932.YNL124W

Structurei

Secondary structure

1492
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi137 – 147Combined sources11
Beta strandi150 – 155Combined sources6
Beta strandi169 – 172Combined sources4
Beta strandi177 – 180Combined sources4
Beta strandi183 – 186Combined sources4
Beta strandi188 – 190Combined sources3
Beta strandi192 – 196Combined sources5
Helixi199 – 201Combined sources3
Helixi202 – 209Combined sources8
Turni210 – 213Combined sources4
Beta strandi214 – 219Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V3MX-ray2.74A/B/C/D/E/F109-232[»]
ProteinModelPortaliP53919
SMRiP53919
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53919

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 230RNA-bindingAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 81Ser-richAdd BLAST27
Compositional biasi361 – 456Pro-richAdd BLAST96
Compositional biasi419 – 488Gln-richAdd BLAST70

Domaini

The central region (136-221) reveals a striking structural homology with the core domain of GAR1.1 Publication

Sequence similaritiesi

Belongs to the NAF1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004697
InParanoidiP53919
KOiK14763
OMAiYHPSYNP
OrthoDBiEOG092C3IL1

Family and domain databases

Gene3Di2.40.10.230, 1 hit
InterProiView protein in InterPro
IPR038664 Gar1/Naf1_Cbf5-bd_sf
IPR007504 H/ACA_rnp_Gar1/Naf1
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF04410 Gar1, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit

Sequencei

Sequence statusi: Complete.

P53919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDLFSKAL ENPDQDLNVE LPKDDVDLGL LGDGGNERKT DEPVADAERS
60 70 80 90 100
TGLGSGSSES ESDSGSDSDS DSGSSGSEDD SADQDVEGED EGGDAIENED
110 120 130 140 150
EDEDPSPSGP ILSKNEILEE TVPELPEDYE ISEKTIITPI GVLKSAFENN
160 170 180 190 200
IIIHATMSGE KRVLKEGSIF CLEDRTLIGM LTEVFGPLQN PFYRIKLPDS
210 220 230 240 250
KKNLFDELKV RLGEKAFIVT PDAHWIDTFE LKRNKGTDAS NGYDEELPEE
260 270 280 290 300
EQEFSDDEKE ALFKKMKKQQ RQRKKRDNRK LANDSDNVKV KRARQPKANS
310 320 330 340 350
LPKLVPPLGM SSNAPMQHGY KSRNARENIK RESSATSNRN GSSPVPITQH
360 370 380 390 400
HQQQFSANNY PFPQQPNGMP YPPYSPFPQP TNFQYPPPPF GQATPAQFSN
410 420 430 440 450
TVPYGSLPPA YNNMSPPTQQ SFMPMTQSQP PLPYGVPPMN QMQNPMYIQP
460 470 480 490
PPQAPPQGNG NFQQVMELHQ ILLQQQQQQH QYQHQHQQDP RT
Length:492
Mass (Da):54,949
Last modified:October 1, 1996 - v1
Checksum:i5A496278070F6255
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti378P → A in strain: YJM269, YJM270, YJM326 and YJM1129. 1 Publication1
Natural varianti423M → I in strain: YJM627. 1 Publication1
Natural varianti424P → S in strain: YJM269, YJM270, YJM326 and YJM1129. 1 Publication1
Natural varianti426T → A in strain: SK1, V1-09, YJM269, YJM270, YJM280, YJM320, YJM326, YJM339, YJM627, YJM789 and YJM1129. 1 Publication1
Natural varianti480H → N in strain: V1-09 and YJM627. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69382 Genomic DNA Translation: CAA93381.1
Z71400 Genomic DNA Translation: CAA96005.1
EF125216 Genomic DNA Translation: ABN58535.1
EF125217 Genomic DNA Translation: ABN58543.1
EF125218 Genomic DNA Translation: ABN58552.1
EF125219 Genomic DNA Translation: ABN58561.1
EF125220 Genomic DNA Translation: ABN58570.1
EF125221 Genomic DNA Translation: ABN58579.1
EF125222 Genomic DNA Translation: ABN58588.1
EF125223 Genomic DNA Translation: ABN58597.1
EF125224 Genomic DNA Translation: ABN58606.1
EF125225 Genomic DNA Translation: ABN58615.1
EF125226 Genomic DNA Translation: ABN58624.1
EF125228 Genomic DNA Translation: ABN58642.1
BK006947 Genomic DNA Translation: DAA10425.1
PIRiS63065
RefSeqiNP_014275.3, NM_001182962.3

Genome annotation databases

EnsemblFungiiYNL124W; YNL124W; YNL124W
GeneIDi855599
KEGGisce:YNL124W

Similar proteinsi

Entry informationi

Entry nameiNAF1_YEAST
AccessioniPrimary (citable) accession number: P53919
Secondary accession number(s): B0KZR2
, B0KZS1, B0KZT9, B0KZZ3, B0L002, D6W159
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Protein nomenclature information
    List of publications dealing with protein nomenclature and links to specialized websites
  2. Forthcoming format changes (XML)
    Announcements of forthcoming format changes in the XML format

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