P53917 (FAR11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Factor arrest protein 11

Gene names

Name:	FAR11
Ordered Locus Names:	YNL127W
ORF Names:	N1221, N1875

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the control of the reentry into the cell cycle following pheromone treatment. Ref.5 Ref.6
Subunit structure	Component of a complex at least composed of FAR3, FAR7, FAR8, FAR10, FAR11 and VPS64.
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the FAR11 family.

Ontologies

Keywords
Biological process	Cell cycle
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intra-S DNA damage checkpoint Inferred from mutant phenotype PubMed 22782902. Source: SGD positive regulation of macroautophagy Inferred from mutant phenotype PubMed 22782902. Source: SGD re-entry into mitotic cell cycle after pheromone arrest Inferred from genetic interaction Ref.6. Source: SGD
Cellular_component	Golgi trans cisterna Inferred from direct assay PubMed 22782902. Source: SGD endoplasmic reticulum Inferred from direct assay PubMed 23625923. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TPD3	P31383	2	EBI-28900,EBI-1936

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Factor arrest protein 11		PRO_0000087190
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.8
	Modified residue	81	1	Phosphoserine Ref.9 Ref.10 Ref.11
	Modified residue	524	1	Phosphoserine Ref.9
	Modified residue	527	1	Phosphoserine Ref.11
	Modified residue	528	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P53917 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0FA7B36B181D97A3	FASTA	953	109,793
	10 20 30 40 50 60 MNASGRSHSK GPIIRSVSLE DLKRNSSFKG NLKYKDEVTS HKEPQVGTLS NEELLKDLDN 70 80 90 100 110 120 MLRGKLNMGR NSFHADKRNK SDGNISALTF KARSGLEGDI RTIDIQQDSS DENDNFKFSD 130 140 150 160 170 180 DGVNKDRNNE KDNNTDNAVE FQDDAEEAEE ENEDESFANV DELDGFDLNK VSDGKHVPIN 190 200 210 220 230 240 EKGEVDYNMP VDKEFQKSLD QCAASLEERS SAPYALQRAV DWELKMFYSL EDELSEWFCS 250 260 270 280 290 300 SDYMHFGQTQ TLFKQKITQP QLFFDDENYA ASVVECLIED IPNSLASNLL ALSYISMGCF 310 320 330 340 350 360 AFTNSKSEHT KIIRRNNLML VPHIQEIVHA FKKIAISCRD DNRNLKKQTI LLFHSSTILY 370 380 390 400 410 420 FICSICIEGR GENPEAVNVV IDAFEKTDLL EFLTKYIENW RWNSRLAMRI RNMISLLFKL 430 440 450 460 470 480 IVLQFGDSSV YKQTKSSIYN LHGLTYPSKH PEKLSISPLH YQAFREDITS RFPDYNMPSS 490 500 510 520 530 540 GLPKDVDKSE SLSQFLEIPR PKSKNPLNMA LIVPEKHIAT PAPSPPSSPQ LMHLGEGPRP 550 560 570 580 590 600 RKSFQTNMAY PCLYPSDNEG SEDDTLEDRI DLNIERKPDN DIVIPFSTEE AARILSESLE 610 620 630 640 650 660 IKLSTKQLWY ERDLFMITER GWKQQLENEP YDYAALNHDA NSSKEEKSAI CIMQRIDKYY 670 680 690 700 710 720 KSCLSSFNSL VFVLLQTMES SLTNNFHRKS EVSDKNLLNM LTPQLEIVRA KELSLKSAAG 730 740 750 760 770 780 ILHALLKWFK LSHILKFEHL AVVIHDSRYI NTCASILSKY SEVYPERVFN KYVQTPNSFW 790 800 810 820 830 840 KECSLSNESY RESYSVDDSG EVDTEIMPSF AYLLRILRKV TGNKTQRLKE LPLSIGILFK 850 860 870 880 890 900 RYYRLFNLDM YHPILKITRE LTPFKNKRWK SEHMELISGV YLYEKLELTD NWVTGKDISG 910 920 930 940 950 ELSDACGQEI ALRALLQFYN FQHYEISMED LGYGHRNSSS QDLLNKESEY LNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames." de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G. Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-953.
[5]	"Identification and characterization of FAR3, a gene required for pheromone-mediated G1 arrest in Saccharomyces cerevisiae." Horecka J., Sprague G.F. Jr. Genetics 144:905-921(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Far3 and five interacting proteins prevent premature recovery from pheromone arrest in the budding yeast Saccharomyces cerevisiae." Kemp H.A., Sprague G.F. Jr. Mol. Cell. Biol. 23:1750-1763(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FAR3; FAR7; FAR8; FAR10 AND VPS64.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81 AND SER-524, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: ADR376.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; SER-527 AND SER-528, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46843 Genomic DNA. Translation: CAA86898.1. Z71402 Genomic DNA. Translation: CAA96008.1. Z71403 Genomic DNA. Translation: CAA96009.1. Z69382 Genomic DNA. Translation: CAA93376.1. BK006947 Genomic DNA. Translation: DAA10422.1.
PIR	S55156.
RefSeq	NP_014272.3. NM_001182965.3.
3D structure databases
ProteinModelPortal	P53917.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35700. 86 interactions.
DIP	DIP-1830N.
IntAct	P53917. 21 interactions.
MINT	MINT-390151.
STRING	4932.YNL127W.
Proteomic databases
MaxQB	P53917.
PaxDb	P53917.
PRIDE	P53917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL127W; YNL127W; YNL127W.
GeneID	855596.
KEGG	sce:YNL127W.
Organism-specific databases
CYGD	YNL127w.
SGD	S000005071. FAR11.
Phylogenomic databases
eggNOG	NOG247682.
GeneTree	ENSGT00400000022095.
HOGENOM	HOG000112500.
OMA	LLQFYNM.
OrthoDB	EOG7RV9QF.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33148-MONOMER.
Gene expression databases
Genevestigator	P53917.
Family and domain databases
InterPro	IPR021819. DUF3402. IPR012486. N1221. [Graphical view]
Pfam	PF11882. DUF3402. 1 hit. PF07923. N1221. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979750.

Entry information

Entry name

FAR11_YEAST

Accession

Primary (citable) accession number: P53917
Secondary accession number(s): D6W156

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program