Reviewed, UniProtKB/Swiss-Prot P53916 (TEP1_YEAST)

Names and origin

Protein names

Recommended name:
    Probable phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TEP1
    EC=3.1.3.67

Gene names

Name:	TEP1
Ordered Locus Names:	YNL128W
ORF Names:	N1220, N1872

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a phosphoinositide 3-phosphatase by regulating PtdIns(3,4,5)P3 levels. Ref.3
Catalytic activity	Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate.
Sequence similarities	Contains 1 phosphatase tensin-type domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ascospore wall assembly Ref.3 Inferred from mutant phenotype. Source: SGD inositol lipid-mediated signaling Ref.3 Inferred from mutant phenotype. Source: SGD phosphoinositide dephosphorylation Ref.3 Inferred from direct assay. Source: SGD protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Probable phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TEP1		PRO_0000215906
Regions
	Domain	33 – 255	223	Phosphatase tensin-type
Sites
	Active site	193	1	Phosphocysteine intermediate Potential

Sequences

	Sequence		Length	Mass (Da)
	P53916-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E56739475D1FA898	FASTA	434	50,152
	10 20 30 40 50 60 MREEGSELEM EKGFLKWKPV NLMKKILSLP MKKTKNDIGL RLDISYILVN LIVCSYPVNT 70 80 90 100 110 120 YPKLLYRNSL DDLILFLTVY HGKGNFRIFN FRGEKEDSDY KDNDLIGIAA KFESKDFEIQ 130 140 150 160 170 180 ELRSTLINDG KIPISPIDLE TRTLVEEETN NVICERIGWL DHFPPPFELL EEIVDGIENY 190 200 210 220 230 240 LSVSKNRVAV LHCRMGKGRS GMITVAYLMK YLQCPLGEAR LIFMQARFKY GMTNGVTIPS 250 260 270 280 290 300 QLRYLRYHEF FITHEKAAQE GISNEAVKFK FKFRLAKMTF LRPSSLITSE SAIVTTKIQH 310 320 330 340 350 360 YNDDRNALLT RKVVYSDIMA HECGGNMTFI FGRDYLTLEN DCRIEFTLGT SKSKAASSII 370 380 390 400 410 420 SWTSCASCWL NIYLETLMHI IKDDSSPDYF QVERLKRDEM LGTTISWQEL DGFGELSTHG 430 LKLFQALKLE WEII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed: 8619318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"TEP1, the yeast homolog of the human tumor suppressor gene PTEN/MMAC1/TEP1, is linked to the phosphatidylinositol pathway and plays a role in the developmental process of sporulation." Heymont J., Berenfeld L., Collins J., Kaganovich A., Maynes B., Moulin A., Ratskovskaya I., Poon P.P., Johnston G.C., Kamenetsky M., DeSilva J., Sun H., Petsko G.A., Engebrecht J. Proc. Natl. Acad. Sci. U.S.A. 97:12672-12677(2000) [PubMed: 11070083] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46843 Genomic DNA. Translation: CAA86897.1. Z71404 Genomic DNA. Translation: CAA96010.1.
PIR	S55155.
RefSeq	NP_014271.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4928N.
IntAct	P53916. 5 interactions.
Proteomic databases
PeptideAtlas	P53916.
PRIDE	P53916.
Genome annotation databases
Ensembl	YNL128W. Saccharomyces cerevisiae. [Contig view]
GeneID	855595.
GenomeReviews	Gene locus YNL128W in contig Y13139_GR.
KEGG	sce:YNL128W.
NMPDR	fig|4932.3.peg.5341.
Organism-specific databases
CYGD	YNL128w.
SGD	S000005072. TEP1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53916.
OMA	P53916. MGKGRSG.
Enzyme and pathway databases
BRENDA	3.1.3.67. 250.
Gene expression databases
GermOnline	YNL128W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014019. Phosphatase_tensin-typ. IPR000340. Tyr_Pase_dual_specific. [Graphical view]
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
PROSITE	PS51181. PPASE_TENSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979747.

Entry information

Entry name

TEP1_YEAST

Accession

Primary (citable) accession number: P53916

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)