UniProtKB - P53915 (NRK1_YEAST)
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Protein
Nicotinamide riboside kinase
Gene
NRK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN).By similarity1 Publication
Miscellaneous
Present with 1460 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide.By similarity
ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide.By similarity
: NAD(+) biosynthesis Pathwayi
This protein is involved in the pathway NAD(+) biosynthesis, which is part of Cofactor biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 20 | MagnesiumBy similarity | 1 | |
Active sitei | 39 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 39 | MagnesiumBy similarity | 1 | |
Binding sitei | 158 | ATPBy similarity | 1 | |
Binding sitei | 159 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 13 – 21 | ATPBy similarity | 9 | |
Nucleotide bindingi | 162 – 164 | ATPBy similarity | 3 | |
Nucleotide bindingi | 208 – 210 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribosylnicotinamide kinase activity Source: SGD
GO - Biological processi
- NAD biosynthesis via nicotinamide riboside salvage pathway Source: SGD
- NAD biosynthetic process Source: SGD
- nicotinamide riboside metabolic process Source: SGD
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Pyridine nucleotide biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER3O-4139. YEAST:MONOMER3O-4139. |
BRENDAi | 2.7.1.22. 984. |
UniPathwayi | UPA00253. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NRK1 Ordered Locus Names:YNL129W ORF Names:N1219, N1870 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL129W. |
SGDi | S000005073. NRK1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215898 | 1 – 240 | Nicotinamide riboside kinaseAdd BLAST | 240 |
Proteomic databases
MaxQBi | P53915. |
PaxDbi | P53915. |
PRIDEi | P53915. |
Interactioni
Protein-protein interaction databases
BioGridi | 35698. 87 interactors. |
DIPi | DIP-8168N. |
IntActi | P53915. 2 interactors. |
STRINGi | 4932.YNL129W. |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 39 – 42 | Substrate bindingBy similarity | 4 | |
Regioni | 59 – 60 | Substrate bindingBy similarity | 2 | |
Regioni | 164 – 165 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | HOG000248838. |
InParanoidi | P53915. |
KOi | K10524. |
OMAi | PQKFARA. |
OrthoDBi | EOG092C4HBE. |
Family and domain databases
InterProi | View protein in InterPro IPR026681. NRK. IPR027417. P-loop_NTPase. |
PANTHERi | PTHR42666:SF1. PTHR42666:SF1. 1 hit. |
SUPFAMi | SSF52540. SSF52540. 2 hits. |
i Sequence
Sequence statusi: Complete.
P53915-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSKKVILVA LSGCSSSGKT TIAKLTASLF TKATLIHEDD FYKHDNEVPV
60 70 80 90 100
DAKYNIQNWD SPEALDFKLF GKELDVIKQT GKIATKLIHN NNVDDPFTKF
110 120 130 140 150
HIDRQVWDEL KAKYDSINDD KYEVVIVDGF MIFNNTGISK KFDLKILVRA
160 170 180 190 200
PYEVLKKRRA SRKGYQTLDS FWVDPPYYFD EFVYESYRAN HAQLFVNGDV
210 220 230 240
EGLLDPRKSK NIKEFINDDD TPIAKPLSWV CQEILKLCKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY611479 Genomic DNA. Translation: AAT11927.1. Z46843 Genomic DNA. Translation: CAA86896.1. Z71405 Genomic DNA. Translation: CAA96011.1. AY558011 Genomic DNA. Translation: AAS56337.1. BK006947 Genomic DNA. Translation: DAA10420.1. |
PIRi | S55154. |
RefSeqi | NP_014270.1. NM_001182967.1. |
Genome annotation databases
EnsemblFungii | YNL129W; YNL129W; YNL129W. |
GeneIDi | 855594. |
KEGGi | sce:YNL129W. |
Similar proteinsi
Entry informationi
Entry namei | NRK1_YEAST | |
Accessioni | P53915Primary (citable) accession number: P53915 Secondary accession number(s): D6W154 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | March 28, 2018 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |