Reviewed, UniProtKB/Swiss-Prot P53915 (NRK1_YEAST)

Names and origin

Protein names

Recommended name:
    Nicotinamide riboside kinase
      Short name=NmR-K
      Short name=NRK
    EC=2.7.1.22
Alternative name(s):
    Ribosylnicotinamide kinase
      Short name=RNK
    Nicotinic acid riboside kinase
    EC=2.7.1.n4
    Ribosylnicotinic acid kinase

Gene names

Name:	NRK1
Ordered Locus Names:	YNL129W
ORF Names:	N1219, N1870

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN) By similarity.
Catalytic activity	ATP + N-ribosylnicotinamide = ADP + nicotinamide ribonucleotide. ATP + D-ribosylnicotinate = ADP + nicotinate D-ribonucleotide.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis.
Miscellaneous	Present with 1460 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the uridine kinase family. NRK subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthesis via nicotinamide riboside salvage pathway Ref.1 Inferred from genetic interaction. Source: SGD nicotinamide riboside metabolic process Ref.1 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nicotinamide riboside kinase activity Ref.1 Inferred from direct assay. Source: SGD ribosylnicotinamide kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Nicotinamide riboside kinase		PRO_0000215898
Regions
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Active site	39	1	Proton acceptor By similarity
	Metal binding	20	1	Magnesium By similarity
	Metal binding	39	1	Magnesium By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	158	1	ATP By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	162	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53915-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F04E99885774CF53	FASTA	240	27,690
	10 20 30 40 50 60 MTSKKVILVA LSGCSSSGKT TIAKLTASLF TKATLIHEDD FYKHDNEVPV DAKYNIQNWD 70 80 90 100 110 120 SPEALDFKLF GKELDVIKQT GKIATKLIHN NNVDDPFTKF HIDRQVWDEL KAKYDSINDD 130 140 150 160 170 180 KYEVVIVDGF MIFNNTGISK KFDLKILVRA PYEVLKKRRA SRKGYQTLDS FWVDPPYYFD 190 200 210 220 230 240 EFVYESYRAN HAQLFVNGDV EGLLDPRKSK NIKEFINDDD TPIAKPLSWV CQEILKLCKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans." Bieganowski P., Brenner C. Cell 117:495-502(2004) [PubMed: 15137942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed: 8619318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY611479 Genomic DNA. Translation: AAT11927.1. Z46843 Genomic DNA. Translation: CAA86896.1. Z71405 Genomic DNA. Translation: CAA96011.1. AY558011 Genomic DNA. Translation: AAS56337.1.
PIR	S55154.
RefSeq	NP_014270.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:8168N.
STRING	P53915.
Genome annotation databases
Ensembl	YNL129W; YNL129W; YNL129W; Saccharomyces cerevisiae. [Genome view]
GeneID	855594.
GenomeReviews	Gene locus YNL129W in contig Y13139_GR.
KEGG	sce:YNL129W.
NMPDR	fig|4932.3.peg.5340.
Organism-specific databases
CYGD	YNL129w.
SGD	S000005073. NRK1.
Phylogenomic databases
HOGENOM	P53915.
OMA	LHEDDFY.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13653.
Gene expression databases
ArrayExpress	P53915.
Genevestigator	P53915.
GermOnline	YNL129W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	979744.

Entry information

Entry name

NRK1_YEAST

Accession

Primary (citable) accession number: P53915

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)