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Protein

Nicotinamide riboside kinase

Gene

NRK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN).By similarity1 Publication

Catalytic activityi

ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide.By similarity
ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide.By similarity

Pathwayi: NAD(+) biosynthesis

This protein is involved in the pathway NAD(+) biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201MagnesiumBy similarity
Active sitei39 – 391Proton acceptorBy similarity
Metal bindingi39 – 391MagnesiumBy similarity
Binding sitei158 – 1581ATPBy similarity
Binding sitei159 – 1591SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 219ATPBy similarity
Nucleotide bindingi162 – 1643ATPBy similarity
Nucleotide bindingi208 – 2103ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ribosylnicotinamide kinase activity Source: SGD

GO - Biological processi

  • NAD biosynthesis via nicotinamide riboside salvage pathway Source: SGD
  • NAD biosynthetic process Source: SGD
  • nicotinamide riboside metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13653.
YEAST:G3O-33150-MONOMER.
YEAST:MONOMER3O-4139.
BRENDAi2.7.1.22. 984.
UniPathwayiUPA00253.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide riboside kinase (EC:2.7.1.22By similarity)
Short name:
NRK
Short name:
NmR-K
Alternative name(s):
Nicotinic acid riboside kinase (EC:2.7.1.173By similarity)
Ribosylnicotinamide kinase
Short name:
RNK
Ribosylnicotinic acid kinase
Gene namesi
Name:NRK1
Ordered Locus Names:YNL129W
ORF Names:N1219, N1870
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL129W.
SGDiS000005073. NRK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Nicotinamide riboside kinasePRO_0000215898Add
BLAST

Proteomic databases

MaxQBiP53915.

Interactioni

Protein-protein interaction databases

BioGridi35698. 29 interactions.
DIPiDIP-8168N.
MINTiMINT-4499900.

Structurei

3D structure databases

ProteinModelPortaliP53915.
SMRiP53915. Positions 5-31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 424Substrate bindingBy similarity
Regioni59 – 602Substrate bindingBy similarity
Regioni164 – 1652Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the uridine kinase family. NRK subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000248838.
InParanoidiP53915.
KOiK10524.
OMAiQDWDSAP.
OrthoDBiEOG7J44KB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR026681. NRK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23404:SF1. PTHR23404:SF1. 2 hits.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P53915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKKVILVA LSGCSSSGKT TIAKLTASLF TKATLIHEDD FYKHDNEVPV
60 70 80 90 100
DAKYNIQNWD SPEALDFKLF GKELDVIKQT GKIATKLIHN NNVDDPFTKF
110 120 130 140 150
HIDRQVWDEL KAKYDSINDD KYEVVIVDGF MIFNNTGISK KFDLKILVRA
160 170 180 190 200
PYEVLKKRRA SRKGYQTLDS FWVDPPYYFD EFVYESYRAN HAQLFVNGDV
210 220 230 240
EGLLDPRKSK NIKEFINDDD TPIAKPLSWV CQEILKLCKD
Length:240
Mass (Da):27,690
Last modified:October 1, 1996 - v1
Checksum:iF04E99885774CF53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY611479 Genomic DNA. Translation: AAT11927.1.
Z46843 Genomic DNA. Translation: CAA86896.1.
Z71405 Genomic DNA. Translation: CAA96011.1.
AY558011 Genomic DNA. Translation: AAS56337.1.
BK006947 Genomic DNA. Translation: DAA10420.1.
PIRiS55154.
RefSeqiNP_014270.1. NM_001182967.1.

Genome annotation databases

EnsemblFungiiYNL129W; YNL129W; YNL129W.
GeneIDi855594.
KEGGisce:YNL129W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY611479 Genomic DNA. Translation: AAT11927.1.
Z46843 Genomic DNA. Translation: CAA86896.1.
Z71405 Genomic DNA. Translation: CAA96011.1.
AY558011 Genomic DNA. Translation: AAS56337.1.
BK006947 Genomic DNA. Translation: DAA10420.1.
PIRiS55154.
RefSeqiNP_014270.1. NM_001182967.1.

3D structure databases

ProteinModelPortaliP53915.
SMRiP53915. Positions 5-31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35698. 29 interactions.
DIPiDIP-8168N.
MINTiMINT-4499900.

Proteomic databases

MaxQBiP53915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL129W; YNL129W; YNL129W.
GeneIDi855594.
KEGGisce:YNL129W.

Organism-specific databases

EuPathDBiFungiDB:YNL129W.
SGDiS000005073. NRK1.

Phylogenomic databases

HOGENOMiHOG000248838.
InParanoidiP53915.
KOiK10524.
OMAiQDWDSAP.
OrthoDBiEOG7J44KB.

Enzyme and pathway databases

UniPathwayiUPA00253.
BioCyciMetaCyc:MONOMER-13653.
YEAST:G3O-33150-MONOMER.
YEAST:MONOMER3O-4139.
BRENDAi2.7.1.22. 984.

Miscellaneous databases

NextBioi979744.
PROiP53915.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR026681. NRK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23404:SF1. PTHR23404:SF1. 2 hits.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans."
    Bieganowski P., Brenner C.
    Cell 117:495-502(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNRK1_YEAST
AccessioniPrimary (citable) accession number: P53915
Secondary accession number(s): D6W154
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1460 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.