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Protein

RNA cytidine acetyltransferase

Gene

KRE33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N4-acetylcytidine (ac4C) at positions 1280 and 1773 in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25086048, PubMed:25653167). Catalyzes the formation of ac4C at position 12 in serine and leucine tRNAs. Requires the tRNA-binding adapter protein TAN1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (PubMed:25653167).UniRule annotation2 Publications

Catalytic activityi

A cytidine in 18S rRNA + ATP + acetyl-CoA + H2O = an N4-acetylcytidine in 18S rRNA + ADP + phosphate + CoA.UniRule annotation2 Publications
A cytidine in tRNA + ATP + acetyl-CoA + H2O = an N4-acetylcytidine in tRNA + ADP + phosphate + CoA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei475ATPUniRule annotationBy similarity1
Binding sitei739Acetyl-CoAUniRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 295ATPUniRule annotationBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processrRNA processing, tRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33152-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA cytidine acetyltransferaseUniRule annotationCurated (EC:2.3.1.-UniRule annotation)
Alternative name(s):
18S rRNA cytosine acetyltransferase1 PublicationUniRule annotation
Killer toxin-resistance protein 331 Publication
Ribosomal RNA cytidine acetyltransferase 11 Publication
Gene namesi
Name:KRE331 Publication
Synonyms:NAT10UniRule annotation, RRA11 Publication
Ordered Locus Names:YNL132WImported
ORF Names:N1216, N1858
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL132W
SGDiS000005076 KRE33

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to a strong inhibition of 18S rRNA synthesis (PubMed:25653167). Deletion results in an altered alkali-soluble beta-glucan phenotype (PubMed:12150911). Heterozygous mutants show haploinsufficiency in K1 killer toxin resistance (PubMed:12663529).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi289K → A: Reduces 18S rRNA acetylation by 80% and tRNA acetylation by 47%. 1 Publication1
Mutagenesisi545H → A: Total loss of 18S rRNA acetylation and tRNA acetylation. 1 Publication1
Mutagenesisi637R → A: Total loss of 18S rRNA acetylation and tRNA acetylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158901 – 1056RNA cytidine acetyltransferaseAdd BLAST1056

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1001PhosphoserineCombined sources1
Modified residuei1007PhosphoserineCombined sources1
Modified residuei1010PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53914
PaxDbiP53914
PRIDEiP53914

PTM databases

iPTMnetiP53914

Interactioni

Subunit structurei

Interacts with TAN1 (PubMed:25653167). Associates with 90S pre-ribosomal particles (PubMed:12150911).UniRule annotation2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35695, 197 interactors
DIPiDIP-6588N
IntActiP53914, 94 interactors
MINTiP53914
STRINGi4932.YNL132W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WLCelectron microscopy3.80LX/LY1-208[»]
ProteinModelPortaliP53914
SMRiP53914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini566 – 706N-acetyltransferaseUniRule annotationAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni638 – 640Acetyl-CoA bindingUniRule annotationBy similarity3
Regioni645 – 651Acetyl-CoA bindingUniRule annotationBy similarity7

Sequence similaritiesi

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000009140
HOGENOMiHOG000210833
InParanoidiP53914
KOiK14521
OMAiGDDEEWN
OrthoDBiEOG092C0F0X

Family and domain databases

HAMAPiMF_03211 RNA_acetyltr_Nat10, 1 hit
InterProiView protein in InterPro
IPR000182 GNAT_dom
IPR007807 Helicase_dom
IPR033688 NAT10
IPR032672 TmcA/NAT10/Kre33
IPR013562 TmcA_N
IPR027992 tRNA_bind_dom
PANTHERiPTHR10925 PTHR10925, 1 hit
PfamiView protein in Pfam
PF08351 DUF1726, 1 hit
PF13718 GNAT_acetyltr_2, 1 hit
PF05127 Helicase_RecD, 1 hit
PF13725 tRNA_bind_2, 1 hit

Sequencei

Sequence statusi: Complete.

P53914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKAIDSRI PSLIRNGVQT KQRSIFVIVG DRARNQLPNL HYLMMSADLK
60 70 80 90 100
MNKSVLWAYK KKLLGFTSHR KKRENKIKKE IKRGTREVNE MDPFESFISN
110 120 130 140 150
QNIRYVYYKE SEKILGNTYG MCILQDFEAL TPNLLARTIE TVEGGGIVVI
160 170 180 190 200
LLKSMSSLKQ LYTMTMDVHA RYRTEAHGDV VARFNERFIL SLGSNPNCLV
210 220 230 240 250
VDDELNVLPL SGAKNVKPLP PKEDDELPPK QLELQELKES LEDVQPAGSL
260 270 280 290 300
VSLSKTVNQA HAILSFIDAI SEKTLNFTVA LTAGRGRGKS AALGISIAAA
310 320 330 340 350
VSHGYSNIFV TSPSPENLKT LFEFIFKGFD ALGYQEHIDY DIIQSTNPDF
360 370 380 390 400
NKAIVRVDIK RDHRQTIQYI VPQDHQVLGQ AELVVIDEAA AIPLPIVKNL
410 420 430 440 450
LGPYLVFMAS TINGYEGTGR SLSLKLIQQL RNQNNTSGRE STQTAVVSRD
460 470 480 490 500
NKEKDSHLHS QSRQLREISL DEPIRYAPGD PIEKWLNKLL CLDVTLIKNP
510 520 530 540 550
RFATRGTPHP SQCNLFVVNR DTLFSYHPVS ENFLEKMMAL YVSSHYKNSP
560 570 580 590 600
NDLQLMSDAP AHKLFVLLPP IDPKDGGRIP DPLCVIQIAL EGEISKESVR
610 620 630 640 650
NSLSRGQRAG GDLIPWLISQ QFQDEEFASL SGARIVRIAT NPEYASMGYG
660 670 680 690 700
SRAIELLRDY FEGKFTDMSE DVRPKDYSIK RVSDKELAKT NLLKDDVKLR
710 720 730 740 750
DAKTLPPLLL KLSEQPPHYL HYLGVSYGLT QSLHKFWKNN SFVPVYLRQT
760 770 780 790 800
ANDLTGEHTC VMLNVLEGRE SNWLVEFAKD FRKRFLSLLS YDFHKFTAVQ
810 820 830 840 850
ALSVIESSKK AQDLSDDEKH DNKELTRTHL DDIFSPFDLK RLDSYSNNLL
860 870 880 890 900
DYHVIGDMIP MLALLYFGDK MGDSVKLSSV QSAILLAIGL QRKNIDTIAK
910 920 930 940 950
ELNLPSNQTI AMFAKIMRKM SQYFRQLLSQ SIEETLPNIK DDAIAEMDGE
960 970 980 990 1000
EIKNYNAAEA LDQMEEDLEE AGSEAVQAMR EKQKELINSL NLDKYAINDN
1010 1020 1030 1040 1050
SEEWAESQKS LEIAAKAKGV VSLKTGKKRT TEKAEDIYRQ EMKAMKKPRK

SKKAAN
Length:1,056
Mass (Da):119,348
Last modified:October 1, 1996 - v1
Checksum:i76721ED0867ED618
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA Translation: CAA86893.1
Z71408 Genomic DNA Translation: CAA96014.1
BK006947 Genomic DNA Translation: DAA10416.1
PIRiS55151
RefSeqiNP_014267.1, NM_001182970.1

Genome annotation databases

EnsemblFungiiYNL132W; YNL132W; YNL132W
GeneIDi855591
KEGGisce:YNL132W

Similar proteinsi

Entry informationi

Entry nameiNAT10_YEAST
AccessioniPrimary (citable) accession number: P53914
Secondary accession number(s): D6W150
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health