P53911 (EAF7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromatin modification-related protein EAF7

Alternative name(s):
ESA1-associated factor 7

Gene names

Name:	EAF7
Ordered Locus Names:	YNL136W
ORF Names:	N1212, N1843

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.
Subunit structure	Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2. Ref.7
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the EAF7 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from direct assay PubMed 16135807. Source: SGD histone acetylation Inferred from physical interaction Ref.7. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.7. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	NuA4 histone acetyltransferase complex Inferred from physical interaction Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-28927,EBI-2042717

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Chromatin modification-related protein EAF7		PRO_0000215882
Regions
	Coiled coil	209 – 310	102	Potential
Amino acid modifications
	Modified residue	143	1	Phosphoserine Ref.10
	Modified residue	167	1	Phosphoserine Ref.10
	Modified residue	200	1	Phosphoserine Ref.10
	Modified residue	225	1	Phosphoserine Ref.8 Ref.9 Ref.10
	Modified residue	393	1	Phosphoserine Ref.4 Ref.9
	Modified residue	397	1	Phosphoserine Ref.4 Ref.9
	Modified residue	411	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P53911 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4C99805F529B4CF1	FASTA	425	49,391
	10 20 30 40 50 60 MVVHWTIVDE IRLLRWASEF KPAGIHKHFH MFCIVERMNS PDKYPVTLLQ KETMKLGKVF 70 80 90 100 110 120 TAKDIWDKLS QSYNLEKIDE MENTYSLEAT TESSRNGNGN GDDAEIHEET LLELNNRIRV 130 140 150 160 170 180 RKQDFTLPWE EYGELILENA RKSPNSNEEY PRVEDMNEKD STIPKESPST DLKNDNNKQE 190 200 210 220 230 240 KNATIKVKEL PEYHTEENDS PIDVQKEPIK EVQSDEKELQ REHMSEEEQK MKSTNKTAAP 250 260 270 280 290 300 VRKSQRLKRS KEVKFEDEEK EEIEEDNTKD EEQKEKKEEI QEPKITHNEE VDKEKNENEE 310 320 330 340 350 360 GDDEREKSTS YENTNGSESE GVDEGVDEEL GYESEREAEG KGKQIESEGG NLKKKTENKK 370 380 390 400 410 420 GDDQQDDTKK DSKDKNEPLA KRTRHSSSTG NTSNETSPKR KRRKAGSRKN SPPATRVSSR LRNKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-397, MASS SPECTROMETRY. Strain: 2124.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4." Krogan N.J., Baetz K., Keogh M.-C., Datta N., Sawa C., Kwok T.C.Y., Thompson N.J., Davey M.G., Pootoolal J., Hughes T.R., Emili A., Buratowski S., Hieter P., Greenblatt J.F. Proc. Natl. Acad. Sci. U.S.A. 101:13513-13518(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-393; SER-397 AND SER-411, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143; SER-167; SER-200 AND SER-225, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46843 Genomic DNA. Translation: CAA86889.1. Z71412 Genomic DNA. Translation: CAA96018.1. BK006947 Genomic DNA. Translation: DAA10412.1.
PIR	S55147.
RefSeq	NP_014263.1. NM_001182974.1.
3D structure databases
ProteinModelPortal	P53911.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53911. 13 interactions.
MINT	MINT-2492018.
STRING	4932.YNL136W.
Proteomic databases
PaxDb	P53911.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL136W; YNL136W; YNL136W.
GeneID	855586.
KEGG	sce:YNL136W.
Organism-specific databases
CYGD	YNL136w.
SGD	S000005080. EAF7.
Phylogenomic databases
eggNOG	NOG279588.
GeneTree	ENSGT00690000102194.
KO	K11399.
OMA	SELANEP.
OrthoDB	EOG4VQF00.
Gene expression databases
Genevestigator	P53911.
GermOnline	YNL136W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012423. EAF7. [Graphical view]
PANTHER	PTHR13581. PTHR13581. 1 hit.
Pfam	PF07904. Eaf7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979721.

Entry information

Entry name

EAF7_YEAST

Accession

Primary (citable) accession number: P53911
Secondary accession number(s): D6W146

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program