Reviewed, UniProtKB/Swiss-Prot P53909 (ADA_YEAST)

Names and origin

Protein names

Recommended name:
    Adenosine deaminase
    EC=3.5.4.4
Alternative name(s):
    Adenosine aminohydrolase

Gene names

Name:	AAH1
Ordered Locus Names:	YNL141W
ORF Names:	N1208, N1825

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3.
Subcellular location	Cytoplasm. Nucleus Ref.4.
Post-translational modification	Probably ubiquitinated when cells enter into quiescence in response to nutrient limitation, since it is specifically degraded via a process requiring the F-box protein SAF1 and components of the SKP1-Cullin-F-box complex.
Miscellaneous	Present with 20700 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adenine catabolic process Inferred from direct assay. Source: SGD hypoxanthine salvage Inferred from direct assay. Source: SGD purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	adenine deaminase activity Inferred from direct assay. Source: SGD adenosine deaminase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38352	1	EBI-2197,EBI-21172

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Adenosine deaminase		PRO_0000194360
Sites
	Active site	204	1	Potential
	Active site	252	1	Potential
	Active site	285	1	Potential
	Active site	286	1	Potential
Experimental info
	Mutagenesis	69	1	I → T in aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
	Mutagenesis	72	1	N → K in aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
	Mutagenesis	219	1	D → G in aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
	Mutagenesis	237	1	E → V in aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
	Mutagenesis	329	1	K → E in aah1-7; impairs AAH1 degradation during postdiauxic growth.

Sequences

	Sequence		Length	Mass (Da)
	P53909-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E25573A9F9EB7BB6	FASTA	347	39,635
	10 20 30 40 50 60 MVSVEFLQEL PKCEHHLHLE GTLEPDLLFP LAKRNDIILP EGFPKSVEEL NEKYKKFRDL 70 80 90 100 110 120 QDFLDYYYIG TNVLISEQDF FDLAWAYFKK VHKQGLVHAE VFYDPQSHTS RGISIETVTK 130 140 150 160 170 180 GFQRACDKAF SEFGITSKLI MCLLRHIEPE ECLKTIEEAT PFIKDGTISA LGLDSAEKPF 190 200 210 220 230 240 PPHLFVECYG KAASLNKDLK LTAHAGEEGP AQFVSDALDL LQVTRIDHGI NSQYDEELLD 250 260 270 280 290 300 RLSRDQTMLT ICPLSNVKLQ VVQSVSELPL QKFLDRDVPF SLNSDDPAYF GGYILDVYTQ 310 320 330 340 VSKDFPHWDH ETWGRIAKNA IKGSWCDDKR KNGLLSRVDE VVTKYSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed: 8619318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sub-families of alpha/beta barrel enzymes: a new adenine deaminase family." Ribard C., Rochet M., Labedan B., Daignan-Fornier B., Alzari P., Scazzocchio C., Oestreicher N. J. Mol. Biol. 334:1117-1131(2003) [PubMed: 14643670] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p." Escusa S., Laporte D., Massoni A., Boucherie H., Dautant A., Daignan-Fornier B. J. Biol. Chem. 282:20097-20103(2007) [PubMed: 17517885] [Abstract] Cited for: FUNCTION, INTERACTION WITH SAF1, UBIQUITINATION, MUTAGENESIS OF ILE-69; ASN-72; ASP-219; GLU-237 AND LYS-329.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46843 Genomic DNA. Translation: CAA86885.1. Z71417 Genomic DNA. Translation: CAA96024.1.
PIR	S55143.
RefSeq	NP_014258.1.
3D structure databases
SMR	P53909. Positions 9-339.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4342N.
IntAct	P53909. 6 interactions.
STRING	P53909.
Proteomic databases
PeptideAtlas	P53909.
Genome annotation databases
Ensembl	YNL141W; YNL141W; YNL141W; Saccharomyces cerevisiae. [Genome view]
GeneID	855581.
KEGG	sce:YNL141W.
NMPDR	fig|4932.3.peg.5326.
Organism-specific databases
CYGD	YNL141w.
SGD	S000005085. AAH1.
Phylogenomic databases
eggNOG	fuNOG04795.
HOGENOM	HBG630382.
OMA	KLIMCLL.
OrthoDB	EOG9B2VDF.
PhylomeDB	P53909.
Enzyme and pathway databases
BRENDA	3.5.4.4. 250.
Gene expression databases
ArrayExpress	P53909.
Genevestigator	P53909.
GermOnline	YNL141W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase. IPR006330. A_deaminase. [Graphical view]
PANTHER	PTHR11409. A/AMP_deaminase. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979709.

Entry information

Entry name

ADA_YEAST

Accession

Primary (citable) accession number: P53909

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)