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Protein

Adenine deaminase

Gene

AAH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N6-substituted adenines that are commonly known as the plant hormones cytokinins.UniRule annotation4 Publications

Miscellaneous

Present with 20700 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Kineticsi

  1. KM=55 µM for adenine1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 30-37 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi16Zinc; catalyticUniRule annotation1
    Metal bindingi18Zinc; catalyticUniRule annotation1
    Metal bindingi204Zinc; catalyticUniRule annotation1
    Active sitei207Proton donorUniRule annotation1
    Sitei228Important for catalytic activityUniRule annotation1
    Metal bindingi285Zinc; catalyticUniRule annotation1
    Binding sitei286SubstrateUniRule annotation1

    GO - Molecular functioni

    • adenine deaminase activity Source: SGD
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • adenine catabolic process Source: SGD
    • hypoxanthine salvage Source: SGD
    • nucleotide metabolic process Source: UniProtKB-KW
    • purine-containing compound salvage Source: SGD
    • purine ribonucleoside monophosphate biosynthetic process Source: InterPro

    Keywordsi

    Molecular functionHydrolase
    Biological processNucleotide metabolism
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:YNL141W-MONOMER.
    BRENDAi3.5.4.2. 984.
    3.5.4.4. 984.
    ReactomeiR-SCE-74217. Purine salvage.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenine deaminaseUniRule annotation (EC:3.5.4.2UniRule annotation)
    Short name:
    ADEUniRule annotation
    Alternative name(s):
    Adenine aminohydrolaseUniRule annotation
    Short name:
    AAHUniRule annotation
    Gene namesi
    Name:AAH1UniRule annotation
    Ordered Locus Names:YNL141W
    ORF Names:N1208, N1825
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIV

    Organism-specific databases

    EuPathDBiFungiDB:YNL141W.
    SGDiS000005085. AAH1.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi69I → T in aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication1
    Mutagenesisi72N → K in aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication1
    Mutagenesisi219D → G in aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication1
    Mutagenesisi237E → V in aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication1
    Mutagenesisi329K → E in aah1-7; impairs AAH1 degradation during postdiauxic growth. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001943601 – 347Adenine deaminaseAdd BLAST347

    Post-translational modificationi

    Probably ubiquitinated when cells enter quiescence in response to nutrient limitation, since it is specifically degraded via a process requiring the F-box protein SAF1 and components of the SKP1-Cullin-F-box complex.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiP53909.
    PRIDEiP53909.

    Expressioni

    Inductioni

    Reduced when grown in a poor nitrogen source medium and strongly down-regulated when cells enter quiescence under nutrient-limiting conditions.1 Publication

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SAF1P383522EBI-2197,EBI-21172

    Protein-protein interaction databases

    BioGridi35686. 273 interactors.
    DIPiDIP-4342N.
    IntActiP53909. 7 interactors.
    MINTiMINT-499127.
    STRINGi4932.YNL141W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53909.
    SMRiP53909.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.UniRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00730000111151.
    InParanoidiP53909.
    KOiK01488.
    OMAiDERLMQR.
    OrthoDBiEOG092C2UZH.

    Family and domain databases

    CDDicd01320. ADA. 1 hit.
    HAMAPiMF_01962. Adenine_deaminase. 1 hit.
    InterProiView protein in InterPro
    IPR006650. A/AMP_deam_AS.
    IPR001365. A/AMP_deaminase_dom.
    IPR028892. ADE.
    IPR006330. Ado/ade_deaminase.
    IPR032466. Metal_Hydrolase.
    PfamiView protein in Pfam
    PF00962. A_deaminase. 1 hit.
    SUPFAMiSSF51556. SSF51556. 1 hit.
    TIGRFAMsiTIGR01430. aden_deam. 1 hit.
    PROSITEiView protein in PROSITE
    PS00485. A_DEAMINASE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53909-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVSVEFLQEL PKCEHHLHLE GTLEPDLLFP LAKRNDIILP EGFPKSVEEL
    60 70 80 90 100
    NEKYKKFRDL QDFLDYYYIG TNVLISEQDF FDLAWAYFKK VHKQGLVHAE
    110 120 130 140 150
    VFYDPQSHTS RGISIETVTK GFQRACDKAF SEFGITSKLI MCLLRHIEPE
    160 170 180 190 200
    ECLKTIEEAT PFIKDGTISA LGLDSAEKPF PPHLFVECYG KAASLNKDLK
    210 220 230 240 250
    LTAHAGEEGP AQFVSDALDL LQVTRIDHGI NSQYDEELLD RLSRDQTMLT
    260 270 280 290 300
    ICPLSNVKLQ VVQSVSELPL QKFLDRDVPF SLNSDDPAYF GGYILDVYTQ
    310 320 330 340
    VSKDFPHWDH ETWGRIAKNA IKGSWCDDKR KNGLLSRVDE VVTKYSH
    Length:347
    Mass (Da):39,635
    Last modified:October 1, 1996 - v1
    Checksum:iE25573A9F9EB7BB6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z46843 Genomic DNA. Translation: CAA86885.1.
    Z71417 Genomic DNA. Translation: CAA96024.1.
    BK006947 Genomic DNA. Translation: DAA10407.1.
    PIRiS55143.
    RefSeqiNP_014258.1. NM_001182979.1.

    Genome annotation databases

    EnsemblFungiiYNL141W; YNL141W; YNL141W.
    GeneIDi855581.
    KEGGisce:YNL141W.

    Similar proteinsi

    Entry informationi

    Entry nameiADE_YEAST
    AccessioniPrimary (citable) accession number: P53909
    Secondary accession number(s): D6W141
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: September 27, 2017
    This is version 146 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names