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P53904 (TBCB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tubulin-specific chaperone B
Alternative name(s):
Alpha-tubulin formation protein 1
Tubulin-folding cofactor B
Gene names
Name:ALF1
Ordered Locus Names:YNL148C
ORF Names:N1201, N1777
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts to sequester alpha-tubulin from interaction with beta-tubulin, raising the possibility that it plays a regulatory role in the formation of the tubulin heterodimer.

Subunit structure

Binds to monomeric alpha-tubulin.

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton. Note: Microtubule-associated.

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TBCB family.

Contains 1 CAP-Gly domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Tubulin-specific chaperone B
PRO_0000083536

Regions

Domain182 – 22544CAP-Gly

Sequences

Sequence LengthMass (Da)Tools
P53904 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BEB01698DB3B3FCC

FASTA25428,354
        10         20         30         40         50         60 
MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD NKEIYSTKKG 

        70         80         90        100        110        120 
GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE GIPSMEVISE EDYLRRDQSV 

       130        140        150        160        170        180 
LRWKMAHGYG RFNAAQQSQR AALAKQDEAY AREQLTAAIG RHCRVTVDGS APREAILRYV 

       190        200        210        220        230        240 
GPLPLDVMGT WCGVEFPEAA GKNDGRINGV TLFGPVAPGH GSFVRPRAVE ILSKDEESAE 

       250 
VEDVHDDVES DDEI 

« Hide

References

« Hide 'large scale' references
[1]"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
Mallet L., Bussereau F., Jacquet M.
Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin."
Feierbach B., Nogales E., Downing K.H., Stearns T.
J. Cell Biol. 144:113-124(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46843 Genomic DNA. Translation: CAA86878.1.
X92517 Genomic DNA. Translation: CAA63291.1.
Z71424 Genomic DNA. Translation: CAA96031.1.
BK006947 Genomic DNA. Translation: DAA10400.1.
PIRS55136.
RefSeqNP_014251.1. NM_001182986.1.

3D structure databases

ProteinModelPortalP53904.
SMRP53904. Positions 1-94, 133-231.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35680. 23 interactions.
DIPDIP-901N.
STRING4932.YNL148C.

Proteomic databases

PaxDbP53904.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL148C; YNL148C; YNL148C.
GeneID855574.
KEGGsce:YNL148C.

Organism-specific databases

CYGDYNL148c.
SGDS000005092. ALF1.

Phylogenomic databases

eggNOGNOG249270.
HOGENOMHOG000209180.
KOK17262.
OMAGWLRYVG.
OrthoDBEOG7MPRSF.

Enzyme and pathway databases

BioCycYEAST:G3O-33166-MONOMER.

Gene expression databases

GenevestigatorP53904.

Family and domain databases

Gene3D2.30.30.190. 1 hit.
InterProIPR000938. CAP-Gly_domain.
IPR000626. Ubiquitin-like.
[Graphical view]
PfamPF01302. CAP_GLY. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
[Graphical view]
SMARTSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMSSF74924. SSF74924. 1 hit.
PROSITEPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979691.

Entry information

Entry nameTBCB_YEAST
AccessionPrimary (citable) accession number: P53904
Secondary accession number(s): D6W134
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families