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P53904

- TBCB_YEAST

UniProt

P53904 - TBCB_YEAST

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Protein

Tubulin-specific chaperone B

Gene

ALF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Acts to sequester alpha-tubulin from interaction with beta-tubulin, raising the possibility that it plays a regulatory role in the formation of the tubulin heterodimer.

GO - Molecular functioni

  1. alpha-tubulin binding Source: SGD
  2. microtubule binding Source: SGD

GO - Biological processi

  1. post-chaperonin tubulin folding pathway Source: SGD
  2. protein folding Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-33166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-specific chaperone B
Alternative name(s):
Alpha-tubulin formation protein 1
Tubulin-folding cofactor B
Gene namesi
Name:ALF1
Ordered Locus Names:YNL148C
ORF Names:N1201, N1777
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL148c.
SGDiS000005092. ALF1.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeleton
Note: Microtubule-associated.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Tubulin-specific chaperone BPRO_0000083536Add
BLAST

Proteomic databases

MaxQBiP53904.
PaxDbiP53904.

Expressioni

Gene expression databases

GenevestigatoriP53904.

Interactioni

Subunit structurei

Binds to monomeric alpha-tubulin.

Protein-protein interaction databases

BioGridi35680. 23 interactions.
DIPiDIP-901N.
STRINGi4932.YNL148C.

Structurei

3D structure databases

ProteinModelPortaliP53904.
SMRiP53904. Positions 133-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini182 – 22544CAP-GlyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TBCB family.Curated
Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG249270.
GeneTreeiENSGT00770000120567.
HOGENOMiHOG000209180.
InParanoidiP53904.
KOiK17262.
OMAiTWCGVEF.
OrthoDBiEOG7MPRSF.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiIPR000938. CAP-Gly_domain.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01302. CAP_GLY. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
[Graphical view]
SMARTiSM01052. CAP_GLY. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEiPS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53904-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD
60 70 80 90 100
NKEIYSTKKG GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE
110 120 130 140 150
GIPSMEVISE EDYLRRDQSV LRWKMAHGYG RFNAAQQSQR AALAKQDEAY
160 170 180 190 200
AREQLTAAIG RHCRVTVDGS APREAILRYV GPLPLDVMGT WCGVEFPEAA
210 220 230 240 250
GKNDGRINGV TLFGPVAPGH GSFVRPRAVE ILSKDEESAE VEDVHDDVES

DDEI
Length:254
Mass (Da):28,354
Last modified:October 1, 1996 - v1
Checksum:iBEB01698DB3B3FCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86878.1.
X92517 Genomic DNA. Translation: CAA63291.1.
Z71424 Genomic DNA. Translation: CAA96031.1.
BK006947 Genomic DNA. Translation: DAA10400.1.
PIRiS55136.
RefSeqiNP_014251.1. NM_001182986.1.

Genome annotation databases

EnsemblFungiiYNL148C; YNL148C; YNL148C.
GeneIDi855574.
KEGGisce:YNL148C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86878.1 .
X92517 Genomic DNA. Translation: CAA63291.1 .
Z71424 Genomic DNA. Translation: CAA96031.1 .
BK006947 Genomic DNA. Translation: DAA10400.1 .
PIRi S55136.
RefSeqi NP_014251.1. NM_001182986.1.

3D structure databases

ProteinModelPortali P53904.
SMRi P53904. Positions 133-231.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35680. 23 interactions.
DIPi DIP-901N.
STRINGi 4932.YNL148C.

Proteomic databases

MaxQBi P53904.
PaxDbi P53904.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL148C ; YNL148C ; YNL148C .
GeneIDi 855574.
KEGGi sce:YNL148C.

Organism-specific databases

CYGDi YNL148c.
SGDi S000005092. ALF1.

Phylogenomic databases

eggNOGi NOG249270.
GeneTreei ENSGT00770000120567.
HOGENOMi HOG000209180.
InParanoidi P53904.
KOi K17262.
OMAi TWCGVEF.
OrthoDBi EOG7MPRSF.

Enzyme and pathway databases

BioCyci YEAST:G3O-33166-MONOMER.

Miscellaneous databases

NextBioi 979691.

Gene expression databases

Genevestigatori P53904.

Family and domain databases

Gene3Di 2.30.30.190. 1 hit.
InterProi IPR000938. CAP-Gly_domain.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF01302. CAP_GLY. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
[Graphical view ]
SMARTi SM01052. CAP_GLY. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEi PS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
    Nasr F., Becam A.-M., Herbert C.J.
    Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin."
    Feierbach B., Nogales E., Downing K.H., Stearns T.
    J. Cell Biol. 144:113-124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTBCB_YEAST
AccessioniPrimary (citable) accession number: P53904
Secondary accession number(s): D6W134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3