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Protein

Tubulin-specific chaperone B

Gene

ALF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts to sequester alpha-tubulin from interaction with beta-tubulin, raising the possibility that it plays a regulatory role in the formation of the tubulin heterodimer.

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • alpha-tubulin binding Source: SGD
  • microtubule binding Source: SGD

GO - Biological processi

  • post-chaperonin tubulin folding pathway Source: SGD
  • protein folding Source: SGD

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-33166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-specific chaperone B
Alternative name(s):
Alpha-tubulin formation protein 1
Tubulin-folding cofactor B
Gene namesi
Name:ALF1
Ordered Locus Names:YNL148C
ORF Names:N1201, N1777
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL148C.
SGDiS000005092. ALF1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835361 – 254Tubulin-specific chaperone BAdd BLAST254

Proteomic databases

MaxQBiP53904.
PRIDEiP53904.

Interactioni

Subunit structurei

Binds to monomeric alpha-tubulin.

GO - Molecular functioni

  • alpha-tubulin binding Source: SGD
  • microtubule binding Source: SGD

Protein-protein interaction databases

BioGridi35680. 53 interactors.
DIPiDIP-901N.
STRINGi4932.YNL148C.

Structurei

3D structure databases

ProteinModelPortaliP53904.
SMRiP53904.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini182 – 225CAP-GlyPROSITE-ProRule annotationAdd BLAST44

Sequence similaritiesi

Belongs to the TBCB family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119173.
HOGENOMiHOG000209180.
InParanoidiP53904.
KOiK17262.
OMAiGWLRYVG.
OrthoDBiEOG092C54ZV.

Family and domain databases

Gene3Di2.30.30.190. 1 hit.
InterProiView protein in InterPro
IPR000938. CAP-Gly_domain.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF01302. CAP_GLY. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
SMARTiView protein in SMART
SM01052. CAP_GLY. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF74924. SSF74924. 1 hit.
PROSITEiView protein in PROSITE
PS00845. CAP_GLY_1. 1 hit.
PS50245. CAP_GLY_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P53904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD
60 70 80 90 100
NKEIYSTKKG GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE
110 120 130 140 150
GIPSMEVISE EDYLRRDQSV LRWKMAHGYG RFNAAQQSQR AALAKQDEAY
160 170 180 190 200
AREQLTAAIG RHCRVTVDGS APREAILRYV GPLPLDVMGT WCGVEFPEAA
210 220 230 240 250
GKNDGRINGV TLFGPVAPGH GSFVRPRAVE ILSKDEESAE VEDVHDDVES

DDEI
Length:254
Mass (Da):28,354
Last modified:October 1, 1996 - v1
Checksum:iBEB01698DB3B3FCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86878.1.
X92517 Genomic DNA. Translation: CAA63291.1.
Z71424 Genomic DNA. Translation: CAA96031.1.
BK006947 Genomic DNA. Translation: DAA10400.1.
PIRiS55136.
RefSeqiNP_014251.1. NM_001182986.1.

Genome annotation databases

EnsemblFungiiYNL148C; YNL148C; YNL148C.
GeneIDi855574.
KEGGisce:YNL148C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTBCB_YEAST
AccessioniPrimary (citable) accession number: P53904
Secondary accession number(s): D6W134
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names