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P53904

- TBCB_YEAST

UniProt

P53904 - TBCB_YEAST

Protein

Tubulin-specific chaperone B

Gene

ALF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Acts to sequester alpha-tubulin from interaction with beta-tubulin, raising the possibility that it plays a regulatory role in the formation of the tubulin heterodimer.

    GO - Molecular functioni

    1. alpha-tubulin binding Source: SGD
    2. microtubule binding Source: SGD

    GO - Biological processi

    1. post-chaperonin tubulin folding pathway Source: SGD
    2. protein folding Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33166-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tubulin-specific chaperone B
    Alternative name(s):
    Alpha-tubulin formation protein 1
    Tubulin-folding cofactor B
    Gene namesi
    Name:ALF1
    Ordered Locus Names:YNL148C
    ORF Names:N1201, N1777
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL148c.
    SGDiS000005092. ALF1.

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton
    Note: Microtubule-associated.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Tubulin-specific chaperone BPRO_0000083536Add
    BLAST

    Proteomic databases

    MaxQBiP53904.
    PaxDbiP53904.

    Expressioni

    Gene expression databases

    GenevestigatoriP53904.

    Interactioni

    Subunit structurei

    Binds to monomeric alpha-tubulin.

    Protein-protein interaction databases

    BioGridi35680. 23 interactions.
    DIPiDIP-901N.
    STRINGi4932.YNL148C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53904.
    SMRiP53904. Positions 133-231.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini182 – 22544CAP-GlyPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TBCB family.Curated
    Contains 1 CAP-Gly domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG249270.
    HOGENOMiHOG000209180.
    KOiK17262.
    OMAiTWCGVEF.
    OrthoDBiEOG7MPRSF.

    Family and domain databases

    Gene3Di2.30.30.190. 1 hit.
    InterProiIPR000938. CAP-Gly_domain.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF01302. CAP_GLY. 1 hit.
    PF14560. Ubiquitin_2. 1 hit.
    [Graphical view]
    SMARTiSM01052. CAP_GLY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    SSF74924. SSF74924. 1 hit.
    PROSITEiPS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53904-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD    50
    NKEIYSTKKG GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE 100
    GIPSMEVISE EDYLRRDQSV LRWKMAHGYG RFNAAQQSQR AALAKQDEAY 150
    AREQLTAAIG RHCRVTVDGS APREAILRYV GPLPLDVMGT WCGVEFPEAA 200
    GKNDGRINGV TLFGPVAPGH GSFVRPRAVE ILSKDEESAE VEDVHDDVES 250
    DDEI 254
    Length:254
    Mass (Da):28,354
    Last modified:October 1, 1996 - v1
    Checksum:iBEB01698DB3B3FCC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46843 Genomic DNA. Translation: CAA86878.1.
    X92517 Genomic DNA. Translation: CAA63291.1.
    Z71424 Genomic DNA. Translation: CAA96031.1.
    BK006947 Genomic DNA. Translation: DAA10400.1.
    PIRiS55136.
    RefSeqiNP_014251.1. NM_001182986.1.

    Genome annotation databases

    EnsemblFungiiYNL148C; YNL148C; YNL148C.
    GeneIDi855574.
    KEGGisce:YNL148C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46843 Genomic DNA. Translation: CAA86878.1 .
    X92517 Genomic DNA. Translation: CAA63291.1 .
    Z71424 Genomic DNA. Translation: CAA96031.1 .
    BK006947 Genomic DNA. Translation: DAA10400.1 .
    PIRi S55136.
    RefSeqi NP_014251.1. NM_001182986.1.

    3D structure databases

    ProteinModelPortali P53904.
    SMRi P53904. Positions 133-231.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35680. 23 interactions.
    DIPi DIP-901N.
    STRINGi 4932.YNL148C.

    Proteomic databases

    MaxQBi P53904.
    PaxDbi P53904.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL148C ; YNL148C ; YNL148C .
    GeneIDi 855574.
    KEGGi sce:YNL148C.

    Organism-specific databases

    CYGDi YNL148c.
    SGDi S000005092. ALF1.

    Phylogenomic databases

    eggNOGi NOG249270.
    HOGENOMi HOG000209180.
    KOi K17262.
    OMAi TWCGVEF.
    OrthoDBi EOG7MPRSF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33166-MONOMER.

    Miscellaneous databases

    NextBioi 979691.

    Gene expression databases

    Genevestigatori P53904.

    Family and domain databases

    Gene3Di 2.30.30.190. 1 hit.
    InterProi IPR000938. CAP-Gly_domain.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF01302. CAP_GLY. 1 hit.
    PF14560. Ubiquitin_2. 1 hit.
    [Graphical view ]
    SMARTi SM01052. CAP_GLY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    SSF74924. SSF74924. 1 hit.
    PROSITEi PS00845. CAP_GLY_1. 1 hit.
    PS50245. CAP_GLY_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
      Mallet L., Bussereau F., Jacquet M.
      Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
      Nasr F., Becam A.-M., Herbert C.J.
      Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin."
      Feierbach B., Nogales E., Downing K.H., Stearns T.
      J. Cell Biol. 144:113-124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTBCB_YEAST
    AccessioniPrimary (citable) accession number: P53904
    Secondary accession number(s): D6W134
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 656 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3