ID   INN1_YEAST              Reviewed;         409 AA.
AC   P53901; D6W131; Q6Q5J6;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 163.
DE   RecName: Full=Ingression protein 1;
GN   Name=INN1; OrderedLocusNames=YNL152W; ORFNames=N1765;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8686380;
RX   DOI=10.1002/(sici)1097-0061(199602)12:2<169::aid-yea894>3.0.co;2-b;
RA   Nasr F., Becam A.-M., Herbert C.J.;
RT   "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24
RT   complete open reading frames: 18 correspond to new genes, one of which
RT   encodes a protein similar to the human myotonic dystrophy kinase.";
RL   Yeast 12:169-175(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CYK2 AND IQG1, AND
RP   MUTAGENESIS OF LYS-28 AND LYS-31.
RX   PubMed=18344988; DOI=10.1038/ncb1701;
RA   Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G.,
RA   Edmondson R., Allen T., Labib K.;
RT   "Inn1 couples contraction of the actomyosin ring to membrane ingression
RT   during cytokinesis in budding yeast.";
RL   Nat. Cell Biol. 10:395-406(2008).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH CYK2 AND CYK3.
RX   PubMed=19528296; DOI=10.1083/jcb.200903125;
RA   Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J.,
RA   Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.;
RT   "Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage
RT   furrow and septum formation in S. cerevisiae.";
RL   J. Cell Biol. 185:995-1012(2009).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH CYK3.
RX   PubMed=19707790; DOI=10.1007/s00438-009-0476-0;
RA   Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J.;
RT   "Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to
RT   the yeast bud neck.";
RL   Mol. Genet. Genomics 282:437-451(2009).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
RN   [9]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20442249; DOI=10.1242/jcs.063891;
RA   Meitinger F., Petrova B., Lombardi I.M., Bertazzi D.T., Hub B.,
RA   Zentgraf H., Pereira G.;
RT   "Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network
RT   regulates cytokinesis in budding yeast.";
RL   J. Cell Sci. 123:1851-1861(2010).
CC   -!- FUNCTION: Required for the ingression of the plasma membrane into the
CC       bud neck at the end of cytokinesis, leading to the separation of the
CC       mother and daughter cells. Stimulates the synthesis of the primary
CC       septum (PS) by CHS2. {ECO:0000269|PubMed:18344988,
CC       ECO:0000269|PubMed:19528296, ECO:0000269|PubMed:19707790}.
CC   -!- SUBUNIT: Interacts with CYK2, CYK3 and IQG1.
CC       {ECO:0000269|PubMed:18344988, ECO:0000269|PubMed:19528296,
CC       ECO:0000269|PubMed:19707790}.
CC   -!- INTERACTION:
CC       P53901; Q05080: HOF1; NbExp=6; IntAct=EBI-28955, EBI-5412;
CC       P53901; P80667: PEX13; NbExp=2; IntAct=EBI-28955, EBI-13206;
CC       P53901; P40073: SHO1; NbExp=4; IntAct=EBI-28955, EBI-18140;
CC   -!- SUBCELLULAR LOCATION: Bud neck {ECO:0000269|PubMed:18344988,
CC       ECO:0000269|PubMed:19528296, ECO:0000269|PubMed:19707790,
CC       ECO:0000269|PubMed:20442249}. Note=Localizes at the contractile
CC       actinomyosin ring at the end of cytokinesis. Recruitment to the bud
CC       neck requires either CYK2 or CYK3.
CC   -!- DOMAIN: The C2 domain is essential for membrane ingression during
CC       cytokinesis, but not for recruitment to the actomyosin ring.
CC       {ECO:0000269|PubMed:18344988}.
CC   -!- SIMILARITY: Belongs to the INN1/fic1 family. {ECO:0000305}.
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DR   EMBL; X92517; CAA63287.1; -; Genomic_DNA.
DR   EMBL; Z71428; CAA96039.1; -; Genomic_DNA.
DR   EMBL; AY558026; AAS56352.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10397.1; -; Genomic_DNA.
DR   PIR; S60975; S60975.
DR   RefSeq; NP_014247.1; NM_001182990.1.
DR   AlphaFoldDB; P53901; -.
DR   SMR; P53901; -.
DR   BioGRID; 35677; 204.
DR   ComplexPortal; CPX-1140; HICS complex.
DR   DIP; DIP-4339N; -.
DR   IntAct; P53901; 12.
DR   MINT; P53901; -.
DR   STRING; 4932.YNL152W; -.
DR   GlyGen; P53901; 3 sites, 1 O-linked glycan (3 sites).
DR   iPTMnet; P53901; -.
DR   MaxQB; P53901; -.
DR   PaxDb; 4932-YNL152W; -.
DR   PeptideAtlas; P53901; -.
DR   TopDownProteomics; P53901; -.
DR   EnsemblFungi; YNL152W_mRNA; YNL152W; YNL152W.
DR   GeneID; 855570; -.
DR   KEGG; sce:YNL152W; -.
DR   AGR; SGD:S000005096; -.
DR   SGD; S000005096; INN1.
DR   VEuPathDB; FungiDB:YNL152W; -.
DR   eggNOG; ENOG502QSUF; Eukaryota.
DR   HOGENOM; CLU_673016_0_0_1; -.
DR   InParanoid; P53901; -.
DR   OMA; TRFHFAN; -.
DR   OrthoDB; 5471322at2759; -.
DR   BioCyc; YEAST:G3O-33169-MONOMER; -.
DR   BioGRID-ORCS; 855570; 8 hits in 10 CRISPR screens.
DR   PRO; PR:P53901; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P53901; Protein.
DR   GO; GO:0005935; C:cellular bud neck; NAS:ComplexPortal.
DR   GO; GO:0000142; C:cellular bud neck contractile ring; IDA:SGD.
DR   GO; GO:0032154; C:cleavage furrow; IMP:CACAO.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0044697; C:HICS complex; IPI:SGD.
DR   GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR   GO; GO:0030234; F:enzyme regulator activity; IGI:SGD.
DR   GO; GO:0051276; P:chromosome organization; IMP:SGD.
DR   GO; GO:0000281; P:mitotic cytokinesis; IMP:SGD.
DR   GO; GO:1902410; P:mitotic cytokinetic process; NAS:ComplexPortal.
DR   GO; GO:1990344; P:secondary cell septum biogenesis; IMP:SGD.
DR   CDD; cd08681; C2_fungal_Inn1p-like; 1.
DR   Gene3D; 2.60.40.150; C2 domain; 1.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR037791; C2_fungal_Inn1.
DR   PANTHER; PTHR47052; CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790); 1.
DR   PANTHER; PTHR47052:SF3; INGRESSION PROTEIN 1; 1.
DR   Pfam; PF00168; C2; 1.
DR   SMART; SM00239; C2; 1.
DR   SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR   PROSITE; PS50004; C2; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Mitosis; Phosphoprotein; Reference proteome.
FT   CHAIN           1..409
FT                   /note="Ingression protein 1"
FT                   /id="PRO_0000203419"
FT   DOMAIN          1..114
FT                   /note="C2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   REGION          300..409
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        362..399
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         392
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MUTAGEN         28
FT                   /note="K->A: Impairs plasma membrane ingression; when
FT                   associated with A-31."
FT                   /evidence="ECO:0000269|PubMed:18344988"
FT   MUTAGEN         31
FT                   /note="K->A: Impairs plasma membrane ingression; when
FT                   associated with A-28."
FT                   /evidence="ECO:0000269|PubMed:18344988"
FT   CONFLICT        221
FT                   /note="D -> G (in Ref. 2; AAS56352)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   409 AA;  46226 MW;  66C2B81D21CE5520 CRC64;
     MSEEVWNGNQ GILSVYVSKA RDLPNLNKLD KQNVMLRLRI AHMTRASNTL HRAGQNPVFH
     YLEKFDITPE IKPLMYVEVY CDRRKKSPLP IGRCEIDLLN AIRADPKEGY CTWYELKRSG
     DEFAGTIFIE LTFTPKVPRL NRDDLNKEMD RLDSSMAMRP IPPLPTESEY DYVHGSTMRQ
     ITPQCVSTSH EDKDEGQPYR NGNVFSMSSK SDTAVLANSN DPIILPPTFS ASMGTTSTLE
     TNDTAISNTS NTKFHFANLR KLKEKINIFK NPDSSTNNCQ NESNKVDIEA LQKAIGVTSL
     SYDEDDDDDD ENDAFYSSSH RVSHNYNQPP LPPIPTRDDM SNYSSSRNTP LVRRDRPSRL
     DSSSPNSHPH PSGLNSPKLP PLPTTSNSNF NSRKNSMSPT RKRPPPRLS
//