P53901 (INN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ingression protein 1

Gene names

Name:	INN1
Ordered Locus Names:	YNL152W
ORF Names:	N1765

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the ingression of the plasma membrane into the bud neck at the end of cytokinesis, leading to the separation of the mother and daughter cells. Stimulates the synthesis of the primary septum (PS) by CHS2. Ref.5 Ref.6 Ref.7
Subunit structure	Interacts with CYK2, CYK3 and IQG1. Ref.5 Ref.6 Ref.7
Subcellular location	Bud neck. Note: Localizes at the contractile actinomyosin ring at the end of cytokinesis. Recruitment to the bud neck requires either CYK2 or CYK3. Ref.5 Ref.6 Ref.7 Ref.9
Domain	The C2 domain is essential for membrane ingression during cytokinesis, but not for recruitment to the actomyosin ring. Ref.5
Sequence similarities	Belongs to the INN1/fic1 family. Contains 1 C2 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly Inferred from mutant phenotype Ref.6. Source: SGD chromosome organization Inferred from mutant phenotype PubMed 18439903. Source: SGD mitotic cytokinesis Inferred from mutant phenotype Ref.5PubMed 22956544. Source: SGD mitotic nuclear division Inferred from electronic annotation. Source: UniProtKB-KW regulation of catalytic activity Inferred from genetic interaction PubMed 22956544. Source: GOC
Cellular_component	HICS complex Inferred from physical interaction PubMed 22623719. Source: SGD cellular bud neck contractile ring Inferred from direct assay Ref.5. Source: SGD
Molecular_function	enzyme regulator activity Inferred from genetic interaction PubMed 22956544. Source: SGD phospholipid binding Inferred from sequence or structural similarity Ref.5. Source: SGD protein binding Inferred from physical interaction PubMed 11283351PubMed 11743162PubMed 18719252PubMed 19841731. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HOF1	Q05080	5	EBI-28955,EBI-5412
PEX13	P80667	2	EBI-28955,EBI-13206

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Ingression protein 1		PRO_0000203419
Regions
	Domain	1 – 134	134	C2
	Compositional bias	303 – 310	8	Poly-Asp
Amino acid modifications
	Modified residue	392	1	Phosphoserine Ref.8
Experimental info
	Mutagenesis	28	1	K → A: Impairs plasma membrane ingression; when associated with A-31. Ref.5
	Mutagenesis	31	1	K → A: Impairs plasma membrane ingression; when associated with A-28. Ref.5
	Sequence conflict	221	1	D → G in AAS56352. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P53901 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 66C2B81D21CE5520	FASTA	409	46,226
	10 20 30 40 50 60 MSEEVWNGNQ GILSVYVSKA RDLPNLNKLD KQNVMLRLRI AHMTRASNTL HRAGQNPVFH 70 80 90 100 110 120 YLEKFDITPE IKPLMYVEVY CDRRKKSPLP IGRCEIDLLN AIRADPKEGY CTWYELKRSG 130 140 150 160 170 180 DEFAGTIFIE LTFTPKVPRL NRDDLNKEMD RLDSSMAMRP IPPLPTESEY DYVHGSTMRQ 190 200 210 220 230 240 ITPQCVSTSH EDKDEGQPYR NGNVFSMSSK SDTAVLANSN DPIILPPTFS ASMGTTSTLE 250 260 270 280 290 300 TNDTAISNTS NTKFHFANLR KLKEKINIFK NPDSSTNNCQ NESNKVDIEA LQKAIGVTSL 310 320 330 340 350 360 SYDEDDDDDD ENDAFYSSSH RVSHNYNQPP LPPIPTRDDM SNYSSSRNTP LVRRDRPSRL 370 380 390 400 DSSSPNSHPH PSGLNSPKLP PLPTTSNSNF NSRKNSMSPT RKRPPPRLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase." Nasr F., Becam A.-M., Herbert C.J. Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast." Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K. Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CYK2 AND IQG1, MUTAGENESIS OF LYS-28 AND LYS-31.
[6]	"Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae." Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E. J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYK2 AND CYK3.
[7]	"Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck." Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J. Mol. Genet. Genomics 282:437-451(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYK3.
[8]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast." Meitinger F., Petrova B., Lombardi I.M., Bertazzi D.T., Hub B., Zentgraf H., Pereira G. J. Cell Sci. 123:1851-1861(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92517 Genomic DNA. Translation: CAA63287.1. Z71428 Genomic DNA. Translation: CAA96039.1. AY558026 Genomic DNA. Translation: AAS56352.1. BK006947 Genomic DNA. Translation: DAA10397.1.
PIR	S60975.
RefSeq	NP_014247.1. NM_001182990.1.
3D structure databases
ProteinModelPortal	P53901.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35677. 19 interactions.
DIP	DIP-4339N.
IntAct	P53901. 9 interactions.
MINT	MINT-496500.
STRING	4932.YNL152W.
Proteomic databases
MaxQB	P53901.
PaxDb	P53901.
PeptideAtlas	P53901.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL152W; YNL152W; YNL152W.
GeneID	855570.
KEGG	sce:YNL152W.
Organism-specific databases
CYGD	YNL152w.
SGD	S000005096. INN1.
Phylogenomic databases
eggNOG	NOG305949.
HOGENOM	HOG000142049.
OMA	INIFKNP.
OrthoDB	EOG7P5TC4.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33169-MONOMER.
Gene expression databases
Genevestigator	P53901.
Family and domain databases
Gene3D	2.60.40.150. 1 hit.
InterPro	IPR000008. C2_dom. [Graphical view]
Pfam	PF00168. C2. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. [Graphical view]
SUPFAM	SSF49562. SSF49562. 1 hit.
ProtoNet	Search...
Other
NextBio	979682.

Entry information

Entry name

INN1_YEAST

Accession

Primary (citable) accession number: P53901
Secondary accession number(s): D6W131, Q6Q5J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program