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P53901

- INN1_YEAST

UniProt

P53901 - INN1_YEAST

Protein

Ingression protein 1

Gene

INN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required for the ingression of the plasma membrane into the bud neck at the end of cytokinesis, leading to the separation of the mother and daughter cells. Stimulates the synthesis of the primary septum (PS) by CHS2.3 Publications

    GO - Molecular functioni

    1. enzyme regulator activity Source: SGD
    2. phospholipid binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. barrier septum assembly Source: SGD
    2. chromosome organization Source: SGD
    3. mitotic cytokinesis Source: SGD
    4. mitotic nuclear division Source: UniProtKB-KW
    5. regulation of catalytic activity Source: GOC

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33169-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ingression protein 1
    Gene namesi
    Name:INN1
    Ordered Locus Names:YNL152W
    ORF Names:N1765
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL152w.
    SGDiS000005096. INN1.

    Subcellular locationi

    Bud neck 4 Publications
    Note: Localizes at the contractile actinomyosin ring at the end of cytokinesis. Recruitment to the bud neck requires either CYK2 or CYK3.

    GO - Cellular componenti

    1. cellular bud neck contractile ring Source: SGD
    2. HICS complex Source: SGD

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi28 – 281K → A: Impairs plasma membrane ingression; when associated with A-31. 1 Publication
    Mutagenesisi31 – 311K → A: Impairs plasma membrane ingression; when associated with A-28. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 409409Ingression protein 1PRO_0000203419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei392 – 3921Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53901.
    PaxDbiP53901.
    PeptideAtlasiP53901.

    Expressioni

    Gene expression databases

    GenevestigatoriP53901.

    Interactioni

    Subunit structurei

    Interacts with CYK2, CYK3 and IQG1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HOF1Q050805EBI-28955,EBI-5412
    PEX13P806672EBI-28955,EBI-13206

    Protein-protein interaction databases

    BioGridi35677. 19 interactions.
    DIPiDIP-4339N.
    IntActiP53901. 9 interactions.
    MINTiMINT-496500.
    STRINGi4932.YNL152W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53901.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 134134C2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi303 – 3108Poly-Asp

    Domaini

    The C2 domain is essential for membrane ingression during cytokinesis, but not for recruitment to the actomyosin ring.1 Publication

    Sequence similaritiesi

    Belongs to the INN1/fic1 family.Curated
    Contains 1 C2 domain.Curated

    Phylogenomic databases

    eggNOGiNOG305949.
    HOGENOMiHOG000142049.
    OMAiINIFKNP.
    OrthoDBiEOG7P5TC4.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR000008. C2_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53901-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEEVWNGNQ GILSVYVSKA RDLPNLNKLD KQNVMLRLRI AHMTRASNTL    50
    HRAGQNPVFH YLEKFDITPE IKPLMYVEVY CDRRKKSPLP IGRCEIDLLN 100
    AIRADPKEGY CTWYELKRSG DEFAGTIFIE LTFTPKVPRL NRDDLNKEMD 150
    RLDSSMAMRP IPPLPTESEY DYVHGSTMRQ ITPQCVSTSH EDKDEGQPYR 200
    NGNVFSMSSK SDTAVLANSN DPIILPPTFS ASMGTTSTLE TNDTAISNTS 250
    NTKFHFANLR KLKEKINIFK NPDSSTNNCQ NESNKVDIEA LQKAIGVTSL 300
    SYDEDDDDDD ENDAFYSSSH RVSHNYNQPP LPPIPTRDDM SNYSSSRNTP 350
    LVRRDRPSRL DSSSPNSHPH PSGLNSPKLP PLPTTSNSNF NSRKNSMSPT 400
    RKRPPPRLS 409
    Length:409
    Mass (Da):46,226
    Last modified:October 1, 1996 - v1
    Checksum:i66C2B81D21CE5520
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti221 – 2211D → G in AAS56352. (PubMed:9169873)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92517 Genomic DNA. Translation: CAA63287.1.
    Z71428 Genomic DNA. Translation: CAA96039.1.
    AY558026 Genomic DNA. Translation: AAS56352.1.
    BK006947 Genomic DNA. Translation: DAA10397.1.
    PIRiS60975.
    RefSeqiNP_014247.1. NM_001182990.1.

    Genome annotation databases

    EnsemblFungiiYNL152W; YNL152W; YNL152W.
    GeneIDi855570.
    KEGGisce:YNL152W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92517 Genomic DNA. Translation: CAA63287.1 .
    Z71428 Genomic DNA. Translation: CAA96039.1 .
    AY558026 Genomic DNA. Translation: AAS56352.1 .
    BK006947 Genomic DNA. Translation: DAA10397.1 .
    PIRi S60975.
    RefSeqi NP_014247.1. NM_001182990.1.

    3D structure databases

    ProteinModelPortali P53901.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35677. 19 interactions.
    DIPi DIP-4339N.
    IntActi P53901. 9 interactions.
    MINTi MINT-496500.
    STRINGi 4932.YNL152W.

    Proteomic databases

    MaxQBi P53901.
    PaxDbi P53901.
    PeptideAtlasi P53901.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL152W ; YNL152W ; YNL152W .
    GeneIDi 855570.
    KEGGi sce:YNL152W.

    Organism-specific databases

    CYGDi YNL152w.
    SGDi S000005096. INN1.

    Phylogenomic databases

    eggNOGi NOG305949.
    HOGENOMi HOG000142049.
    OMAi INIFKNP.
    OrthoDBi EOG7P5TC4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33169-MONOMER.

    Miscellaneous databases

    NextBioi 979682.

    Gene expression databases

    Genevestigatori P53901.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR000008. C2_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
      Nasr F., Becam A.-M., Herbert C.J.
      Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast."
      Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K.
      Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CYK2 AND IQG1, MUTAGENESIS OF LYS-28 AND LYS-31.
    6. "Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
      Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
      J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYK2 AND CYK3.
    7. "Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck."
      Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J.
      Mol. Genet. Genomics 282:437-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYK3.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast."
      Meitinger F., Petrova B., Lombardi I.M., Bertazzi D.T., Hub B., Zentgraf H., Pereira G.
      J. Cell Sci. 123:1851-1861(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiINN1_YEAST
    AccessioniPrimary (citable) accession number: P53901
    Secondary accession number(s): D6W131, Q6Q5J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3