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P53901 (INN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ingression protein 1
Gene names
Name:INN1
Ordered Locus Names:YNL152W
ORF Names:N1765
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the ingression of the plasma membrane into the bud neck at the end of cytokinesis, leading to the separation of the mother and daughter cells. Stimulates the synthesis of the primary septum (PS) by CHS2. Ref.5 Ref.6 Ref.7

Subunit structure

Interacts with CYK2, CYK3 and IQG1. Ref.5 Ref.6 Ref.7

Subcellular location

Bud neck. Note: Localizes at the contractile actinomyosin ring at the end of cytokinesis. Recruitment to the bud neck requires either CYK2 or CYK3. Ref.5 Ref.6 Ref.7 Ref.9

Domain

The C2 domain is essential for membrane ingression during cytokinesis, but not for recruitment to the actomyosin ring. Ref.5

Sequence similarities

Belongs to the INN1/fic1 family.

Contains 1 C2 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HOF1Q050805EBI-28955,EBI-5412
PEX13P806672EBI-28955,EBI-13206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409Ingression protein 1
PRO_0000203419

Regions

Domain1 – 134134C2
Compositional bias303 – 3108Poly-Asp

Amino acid modifications

Modified residue3921Phosphoserine Ref.8

Experimental info

Mutagenesis281K → A: Impairs plasma membrane ingression; when associated with A-31. Ref.5
Mutagenesis311K → A: Impairs plasma membrane ingression; when associated with A-28. Ref.5
Sequence conflict2211D → G in AAS56352. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P53901 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 66C2B81D21CE5520

FASTA40946,226
        10         20         30         40         50         60 
MSEEVWNGNQ GILSVYVSKA RDLPNLNKLD KQNVMLRLRI AHMTRASNTL HRAGQNPVFH 

        70         80         90        100        110        120 
YLEKFDITPE IKPLMYVEVY CDRRKKSPLP IGRCEIDLLN AIRADPKEGY CTWYELKRSG 

       130        140        150        160        170        180 
DEFAGTIFIE LTFTPKVPRL NRDDLNKEMD RLDSSMAMRP IPPLPTESEY DYVHGSTMRQ 

       190        200        210        220        230        240 
ITPQCVSTSH EDKDEGQPYR NGNVFSMSSK SDTAVLANSN DPIILPPTFS ASMGTTSTLE 

       250        260        270        280        290        300 
TNDTAISNTS NTKFHFANLR KLKEKINIFK NPDSSTNNCQ NESNKVDIEA LQKAIGVTSL 

       310        320        330        340        350        360 
SYDEDDDDDD ENDAFYSSSH RVSHNYNQPP LPPIPTRDDM SNYSSSRNTP LVRRDRPSRL 

       370        380        390        400 
DSSSPNSHPH PSGLNSPKLP PLPTTSNSNF NSRKNSMSPT RKRPPPRLS 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast."
Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K.
Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CYK2 AND IQG1, MUTAGENESIS OF LYS-28 AND LYS-31.
[6]"Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYK2 AND CYK3.
[7]"Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck."
Jendretzki A., Ciklic I., Rodicio R., Schmitz H.P., Heinisch J.J.
Mol. Genet. Genomics 282:437-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CYK3.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast."
Meitinger F., Petrova B., Lombardi I.M., Bertazzi D.T., Hub B., Zentgraf H., Pereira G.
J. Cell Sci. 123:1851-1861(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92517 Genomic DNA. Translation: CAA63287.1.
Z71428 Genomic DNA. Translation: CAA96039.1.
AY558026 Genomic DNA. Translation: AAS56352.1.
BK006947 Genomic DNA. Translation: DAA10397.1.
PIRS60975.
RefSeqNP_014247.1. NM_001182990.1.

3D structure databases

ProteinModelPortalP53901.
SMRP53901. Positions 10-135.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35677. 19 interactions.
DIPDIP-4339N.
IntActP53901. 9 interactions.
MINTMINT-496500.
STRING4932.YNL152W.

Proteomic databases

PaxDbP53901.
PeptideAtlasP53901.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL152W; YNL152W; YNL152W.
GeneID855570.
KEGGsce:YNL152W.

Organism-specific databases

CYGDYNL152w.
SGDS000005096. INN1.

Phylogenomic databases

eggNOGNOG305949.
HOGENOMHOG000142049.
OMAINIFKNP.
OrthoDBEOG7P5TC4.

Enzyme and pathway databases

BioCycYEAST:G3O-33169-MONOMER.

Gene expression databases

GenevestigatorP53901.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
InterProIPR000008. C2_dom.
[Graphical view]
PfamPF00168. C2. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
ProtoNetSearch...

Other

NextBio979682.

Entry information

Entry nameINN1_YEAST
AccessionPrimary (citable) accession number: P53901
Secondary accession number(s): D6W131, Q6Q5J6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families