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Protein

Prefoldin subunit 4

Gene

GIM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Miscellaneous

Present with 7470 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chaperone binding Source: GO_Central
  • tubulin binding Source: SGD
  • unfolded protein binding Source: InterPro

GO - Biological processi

  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein folding Source: GO_Central
  • tubulin complex assembly Source: SGD
  • ubiquitin-dependent catabolism of misfolded proteins by cytoplasm-associated proteasome Source: SGD

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-33170-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prefoldin subunit 4
Alternative name(s):
Genes involved in microtubule biogenesis protein 3
Gim complex subunit 3
Short name:
GimC subunit 3
Gene namesi
Name:GIM3
Synonyms:PFD4
Ordered Locus Names:YNL153C
ORF Names:N1761
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL153C.
SGDiS000005097. GIM3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • prefoldin complex Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001248481 – 129Prefoldin subunit 4Add BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53900.
PRIDEiP53900.

Interactioni

Subunit structurei

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PAC10P483634EBI-13246,EBI-13239

GO - Molecular functioni

  • chaperone binding Source: GO_Central
  • tubulin binding Source: SGD
  • unfolded protein binding Source: InterPro

Protein-protein interaction databases

BioGridi35676. 729 interactors.
DIPiDIP-3000N.
IntActiP53900. 11 interactors.
MINTiMINT-434964.
STRINGi4932.YNL153C.

Structurei

3D structure databases

ProteinModelPortaliP53900.
SMRiP53900.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prefoldin subunit beta family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006696.
HOGENOMiHOG000204477.
InParanoidiP53900.
KOiK09550.
OMAiMTQLKVE.
OrthoDBiEOG092C5KAA.

Family and domain databases

InterProiView protein in InterPro
IPR002777. PFD_beta-like.
IPR016661. PFDN4.
PfamiView protein in Pfam
PF01920. Prefoldin_2. 1 hit.
PIRSFiPIRSF016477. Prefoldin_subunit_4. 1 hit.

Sequencei

Sequence statusi: Complete.

P53900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLPQGQRN NTQVTFEDQQ KINEFSKLIM RKDAIAQELS LQREEKEYLD
60 70 80 90 100
DVSLEIELID EDEPVQYKVG DLFIFMKQSK VTAQLEKDAE RLDNKIETLE
110 120
DKQRDIDSRL DALKAILYAK FGDNINLER
Length:129
Mass (Da):15,180
Last modified:October 1, 1996 - v1
Checksum:i4738F9EA813E04A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63286.1.
Z71429 Genomic DNA. Translation: CAA96040.1.
AY692812 Genomic DNA. Translation: AAT92831.1.
BK006947 Genomic DNA. Translation: DAA10396.1.
PIRiS60974.
RefSeqiNP_014246.1. NM_001182991.1.

Genome annotation databases

EnsemblFungiiYNL153C; YNL153C; YNL153C.
GeneIDi855569.
KEGGisce:YNL153C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPFD4_YEAST
AccessioniPrimary (citable) accession number: P53900
Secondary accession number(s): D6W130
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names