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P53900 (PFD4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prefoldin subunit 4
Alternative name(s):
Genes involved in microtubule biogenesis protein 3
Gim complex subunit 3
Short name=GimC subunit 3
Gene names
Name:GIM3
Synonyms:PFD4
Ordered Locus Names:YNL153C
ORF Names:N1761
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length129 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Subunit structure

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Ref.6

Miscellaneous

Present with 7470 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the prefoldin subunit beta family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PAC10P483634EBI-13246,EBI-13239

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 129129Prefoldin subunit 4
PRO_0000124848

Amino acid modifications

Modified residue11N-acetylmethionine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P53900 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 4738F9EA813E04A9

FASTA12915,180
        10         20         30         40         50         60 
MELLPQGQRN NTQVTFEDQQ KINEFSKLIM RKDAIAQELS LQREEKEYLD DVSLEIELID 

        70         80         90        100        110        120 
EDEPVQYKVG DLFIFMKQSK VTAQLEKDAE RLDNKIETLE DKQRDIDSRL DALKAILYAK 


FGDNINLER 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
Geissler S., Siegers K., Schiebel E.
EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92517 Genomic DNA. Translation: CAA63286.1.
Z71429 Genomic DNA. Translation: CAA96040.1.
AY692812 Genomic DNA. Translation: AAT92831.1.
BK006947 Genomic DNA. Translation: DAA10396.1.
PIRS60974.
RefSeqNP_014246.1. NM_001182991.1.

3D structure databases

ProteinModelPortalP53900.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35676. 409 interactions.
DIPDIP-3000N.
IntActP53900. 11 interactions.
MINTMINT-434964.
STRING4932.YNL153C.

Proteomic databases

PaxDbP53900.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL153C; YNL153C; YNL153C.
GeneID855569.
KEGGsce:YNL153C.

Organism-specific databases

CYGDYNL153c.
SGDS000005097. GIM3.

Phylogenomic databases

eggNOGCOG1382.
GeneTreeENSGT00390000006696.
HOGENOMHOG000204477.
KOK09550.
OMAYAKFGDN.
OrthoDBEOG735492.

Enzyme and pathway databases

BioCycYEAST:G3O-33170-MONOMER.

Gene expression databases

GenevestigatorP53900.

Family and domain databases

Gene3D1.10.287.370. 1 hit.
InterProIPR002777. PFD_beta-like.
IPR016661. PFDN4.
IPR009053. Prefoldin.
[Graphical view]
PANTHERPTHR21100. PTHR21100. 1 hit.
PfamPF01920. Prefoldin_2. 1 hit.
[Graphical view]
PIRSFPIRSF016477. Prefoldin_subunit_4. 1 hit.
SUPFAMSSF46579. SSF46579. 1 hit.
ProtoNetSearch...

Other

NextBio979679.
PROP53900.

Entry information

Entry namePFD4_YEAST
AccessionPrimary (citable) accession number: P53900
Secondary accession number(s): D6W130
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families