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P53900

- PFD4_YEAST

UniProt

P53900 - PFD4_YEAST

Protein

Prefoldin subunit 4

Gene

GIM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. tubulin binding Source: SGD

    GO - Biological processi

    1. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
    2. protein folding Source: InterPro
    3. tubulin complex assembly Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33170-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prefoldin subunit 4
    Alternative name(s):
    Genes involved in microtubule biogenesis protein 3
    Gim complex subunit 3
    Short name:
    GimC subunit 3
    Gene namesi
    Name:GIM3
    Synonyms:PFD4
    Ordered Locus Names:YNL153C
    ORF Names:N1761
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL153c.
    SGDiS000005097. GIM3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. prefoldin complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 129129Prefoldin subunit 4PRO_0000124848Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP53900.
    PaxDbiP53900.

    Expressioni

    Gene expression databases

    GenevestigatoriP53900.

    Interactioni

    Subunit structurei

    Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAC10P483634EBI-13246,EBI-13239

    Protein-protein interaction databases

    BioGridi35676. 410 interactions.
    DIPiDIP-3000N.
    IntActiP53900. 11 interactions.
    MINTiMINT-434964.
    STRINGi4932.YNL153C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53900.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the prefoldin subunit beta family.Curated

    Phylogenomic databases

    eggNOGiCOG1382.
    GeneTreeiENSGT00390000006696.
    HOGENOMiHOG000204477.
    KOiK09550.
    OMAiYAKFGDN.
    OrthoDBiEOG735492.

    Family and domain databases

    Gene3Di1.10.287.370. 1 hit.
    InterProiIPR002777. PFD_beta-like.
    IPR016661. PFDN4.
    IPR009053. Prefoldin.
    [Graphical view]
    PANTHERiPTHR21100. PTHR21100. 1 hit.
    PfamiPF01920. Prefoldin_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016477. Prefoldin_subunit_4. 1 hit.
    SUPFAMiSSF46579. SSF46579. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53900-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELLPQGQRN NTQVTFEDQQ KINEFSKLIM RKDAIAQELS LQREEKEYLD    50
    DVSLEIELID EDEPVQYKVG DLFIFMKQSK VTAQLEKDAE RLDNKIETLE 100
    DKQRDIDSRL DALKAILYAK FGDNINLER 129
    Length:129
    Mass (Da):15,180
    Last modified:October 1, 1996 - v1
    Checksum:i4738F9EA813E04A9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92517 Genomic DNA. Translation: CAA63286.1.
    Z71429 Genomic DNA. Translation: CAA96040.1.
    AY692812 Genomic DNA. Translation: AAT92831.1.
    BK006947 Genomic DNA. Translation: DAA10396.1.
    PIRiS60974.
    RefSeqiNP_014246.1. NM_001182991.1.

    Genome annotation databases

    EnsemblFungiiYNL153C; YNL153C; YNL153C.
    GeneIDi855569.
    KEGGisce:YNL153C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92517 Genomic DNA. Translation: CAA63286.1 .
    Z71429 Genomic DNA. Translation: CAA96040.1 .
    AY692812 Genomic DNA. Translation: AAT92831.1 .
    BK006947 Genomic DNA. Translation: DAA10396.1 .
    PIRi S60974.
    RefSeqi NP_014246.1. NM_001182991.1.

    3D structure databases

    ProteinModelPortali P53900.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35676. 410 interactions.
    DIPi DIP-3000N.
    IntActi P53900. 11 interactions.
    MINTi MINT-434964.
    STRINGi 4932.YNL153C.

    Proteomic databases

    MaxQBi P53900.
    PaxDbi P53900.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL153C ; YNL153C ; YNL153C .
    GeneIDi 855569.
    KEGGi sce:YNL153C.

    Organism-specific databases

    CYGDi YNL153c.
    SGDi S000005097. GIM3.

    Phylogenomic databases

    eggNOGi COG1382.
    GeneTreei ENSGT00390000006696.
    HOGENOMi HOG000204477.
    KOi K09550.
    OMAi YAKFGDN.
    OrthoDBi EOG735492.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33170-MONOMER.

    Miscellaneous databases

    NextBioi 979679.
    PROi P53900.

    Gene expression databases

    Genevestigatori P53900.

    Family and domain databases

    Gene3Di 1.10.287.370. 1 hit.
    InterProi IPR002777. PFD_beta-like.
    IPR016661. PFDN4.
    IPR009053. Prefoldin.
    [Graphical view ]
    PANTHERi PTHR21100. PTHR21100. 1 hit.
    Pfami PF01920. Prefoldin_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016477. Prefoldin_subunit_4. 1 hit.
    SUPFAMi SSF46579. SSF46579. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
      Nasr F., Becam A.-M., Herbert C.J.
      Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "A novel protein complex promoting formation of functional alpha- and gamma-tubulin."
      Geissler S., Siegers K., Schiebel E.
      EMBO J. 17:952-966(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system."
      Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E., Hartl F.U.
      EMBO J. 18:75-84(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PREFOLDIN COMPLEX.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPFD4_YEAST
    AccessioniPrimary (citable) accession number: P53900
    Secondary accession number(s): D6W130
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7470 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3