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Protein

CDC48-associated ubiquitin-like/zinc finger protein 1

Gene

CUZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in the ubiquitin-proteasome system (UPS) protecting cells from metalloid-induced proteotoxicity.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri15 – 5844AN1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. proteasome binding Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to arsenic-containing substance Source: SGD
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CDC48-associated ubiquitin-like/zinc finger protein 11 Publication
Short name:
CDC48-associated UBL/Zn-finger protein 11 Publication
Gene namesi
Name:CUZ11 Publication
Ordered Locus Names:YNL155WImported
ORF Names:N1751
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL155w.
SGDiS000005099. CUZ1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274CDC48-associated ubiquitin-like/zinc finger protein 1PRO_0000203418Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei273 – 2731Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53899.
PaxDbiP53899.

Expressioni

Inductioni

By transcriptional activator RPN4.1 Publication

Gene expression databases

GenevestigatoriP53899.

Interactioni

Subunit structurei

Interacts (via its ubiquitin-like domain) with CDC48 (via N-terminus). Associates with the 26S proteasome. Specifically interacts with the regulatory particle (RP) subunit RPN2. Exposure to arsenite, a known inducer of protein misfolding resulting in accumulation of polyubiquitinated conjugates, enhances the association with the proteoasome. Binds to ubiquitinated proteins conjugated to a 4 or more molecule ubiquitin chain. Binding to ubiquitinated proteins is zinc-dependent.2 Publications

Protein-protein interaction databases

BioGridi35674. 63 interactions.
DIPiDIP-1796N.
IntActiP53899. 6 interactions.
MINTiMINT-398748.
STRINGi4932.YNL155W.

Structurei

3D structure databases

ProteinModelPortaliP53899.
SMRiP53899. Positions 12-75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 26697Ubiquitin-like1 PublicationAdd
BLAST

Sequence similaritiesi

Contains 1 AN1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri15 – 5844AN1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG3582.
GeneTreeiENSGT00730000111180.
HOGENOMiHOG000248569.
InParanoidiP53899.
KOiK07059.
OMAiNDRIYIW.
OrthoDBiEOG7HF1Z1.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR000058. Znf_AN1.
[Graphical view]
PfamiPF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51039. ZF_AN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53899-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAAIEKETG MLDVGKHCAY CRQLDFLPFH CSFCNEDFCS NHRLKEDHHC
60 70 80 90 100
RWLLEHEEVH KTEKSPSKSR DGSSSNDEAY FKSLLPERAS VRIQRVSETR
110 120 130 140 150
EPLRGSNTAK VSSTLNSKTL DKIFKFFQRN EKRKSNNKSK KNFGSSSNKI
160 170 180 190 200
IQLANLKKIA KGDPKIPMQN RIYIWCYLVD GDETDIAKED TRMPLYINKM
210 220 230 240 250
WPVGRAMDYL SIQLNVKSST LTNSSSNDKF QLCKLKEGKQ VSFYNIGASL
260 270
RVTNEIKDLD TLYLVHNNAD EKSN
Length:274
Mass (Da):31,517
Last modified:October 1, 1996 - v1
Checksum:i1BC3E0D932AC1365
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63284.1.
Z71431 Genomic DNA. Translation: CAA96042.1.
AY692693 Genomic DNA. Translation: AAT92712.1.
BK006947 Genomic DNA. Translation: DAA10394.1.
PIRiS60972.
RefSeqiNP_014244.1. NM_001182993.1.

Genome annotation databases

EnsemblFungiiYNL155W; YNL155W; YNL155W.
GeneIDi855567.
KEGGisce:YNL155W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63284.1.
Z71431 Genomic DNA. Translation: CAA96042.1.
AY692693 Genomic DNA. Translation: AAT92712.1.
BK006947 Genomic DNA. Translation: DAA10394.1.
PIRiS60972.
RefSeqiNP_014244.1. NM_001182993.1.

3D structure databases

ProteinModelPortaliP53899.
SMRiP53899. Positions 12-75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35674. 63 interactions.
DIPiDIP-1796N.
IntActiP53899. 6 interactions.
MINTiMINT-398748.
STRINGi4932.YNL155W.

Proteomic databases

MaxQBiP53899.
PaxDbiP53899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL155W; YNL155W; YNL155W.
GeneIDi855567.
KEGGisce:YNL155W.

Organism-specific databases

CYGDiYNL155w.
SGDiS000005099. CUZ1.

Phylogenomic databases

eggNOGiCOG3582.
GeneTreeiENSGT00730000111180.
HOGENOMiHOG000248569.
InParanoidiP53899.
KOiK07059.
OMAiNDRIYIW.
OrthoDBiEOG7HF1Z1.

Enzyme and pathway databases

BioCyciYEAST:G3O-33171-MONOMER.

Miscellaneous databases

NextBioi979673.
PROiP53899.

Gene expression databases

GenevestigatoriP53899.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR000058. Znf_AN1.
[Graphical view]
PfamiPF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51039. ZF_AN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
    Nasr F., Becam A.-M., Herbert C.J.
    Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A conserved protein with AN1 zinc finger and ubiquitin-like domains modulates Cdc48 (p97) function in the ubiquitin-proteasome pathway."
    Sa-Moura B., Funakoshi M., Tomko R.J. Jr., Dohmen R.J., Wu Z., Peng J., Hochstrasser M.
    J. Biol. Chem. 288:33682-33696(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC48 AND THE PROTEASOME.
  10. "Cuz1/Ynl155w, a zinc-dependent ubiquitin-binding protein, protects cells from metalloid-induced proteotoxicity."
    Hanna J., Waterman D., Isasa M., Elsasser S., Shi Y., Gygi S., Finley D.
    J. Biol. Chem. 289:1876-1885(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC48; RPN2; THE PROTEASOME AND UBIQUITINATED PROTEINS, INDUCTION BY RPN4.

Entry informationi

Entry nameiCUZ1_YEAST
AccessioniPrimary (citable) accession number: P53899
Secondary accession number(s): D6W128
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.