P53895 (ASI2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ASI2

Alternative name(s):
Amino acid sensor-independent protein 2

Gene names

Name:	ASI2
Ordered Locus Names:	YNL159C
ORF Names:	N1735

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of SPS-sensor signaling. Together with ASI1 and ASI3, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals. Ref.5
Subcellular location	Nucleus inner membrane; Multi-pass membrane protein Ref.5.
Post-translational modification	Not glycosylated. Ref.5

Ontologies

Keywords
Cellular component	Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from genetic interaction PubMed 11454748. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear inner membrane Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Protein ASI2		PRO_0000203414
Regions
	Topological domain	1 – 222	222	Nuclear Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Topological domain	244 – 247	4	Perinuclear space Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Topological domain	269 – 289	21	Nuclear Potential

Sequences

	Sequence		Length	Mass (Da)
	P53895 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 95C425488F1E3724	FASTA	289	32,986
	10 20 30 40 50 60 MARPQNHRRS NWTERDDNDD YLFQRFLEES ETRHSREPSP VTEQSQQELQ QDVQQAIDGI 70 80 90 100 110 120 FNSLRRNMSS TSNINRAANM DATTNGNGGI NADTIRATNA NTADSPFTAR QQSPLRTFLR 130 140 150 160 170 180 NLFILDYFIG LILFPFSVYN ILRSGFNSMT FSENDFIIEI VGYWKFAKIF GSGGTTLIAY 190 200 210 220 230 240 KDTGKLGLLG KFHNIIVFYS SPVIKHIMKS RDGNEPNLNW IRLMFAKAFE LFVKVSTILI 250 260 270 280 YLAYGVSGTV YMVTAGFFFV LCLLFTVIRR YKGVHRMLVS QRITGPGVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase." Nasr F., Becam A.-M., Herbert C.J. Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[5]	"Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2." Zargari A., Boban M., Heessen S., Andreasson C., Thyberg J., Ljungdahl P.O. J. Biol. Chem. 282:594-605(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, LACK OF GLYCOSYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92517 Genomic DNA. Translation: CAA63280.1. Z71435 Genomic DNA. Translation: CAA96046.1. BK006947 Genomic DNA. Translation: DAA10390.1.
PIR	S60968.
RefSeq	NP_014240.1. NM_001182997.1.
3D structure databases
ProteinModelPortal	P53895.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4337N.
IntAct	P53895. 1 interaction.
MINT	MINT-484885.
STRING	4932.YNL159C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL159C; YNL159C; YNL159C.
GeneID	855563.
KEGG	sce:YNL159C.
Organism-specific databases
CYGD	YNL159c.
SGD	S000005103. ASI2.
Phylogenomic databases
eggNOG	NOG46201.
HOGENOM	HOG000000852.
OMA	KFHNIIV.
OrthoDB	EOG40KC8H.
Gene expression databases
Genevestigator	P53895.
GermOnline	YNL159C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979661.

Entry information

Entry name

ASI2_YEAST

Accession

Primary (citable) accession number: P53895
Secondary accession number(s): D6W124

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program