Serine/threonine-protein kinase CBK1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P53894 (CBK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase CBK1
EC=2.7.11.1

Alternative name(s):
Cell wall biosynthesis kinase

Gene names

Name:	CBK1
Ordered Locus Names:	YNL161W
ORF Names:	N1727

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein kinase that seems to play a role in the regulation of cell morphogenesis and proliferation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Associates with PAG1/TAO3 and interacts with MOB2. Ref.4 Ref.5
Miscellaneous	Present with 450 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	budding cell apical bud growth Inferred from mutant phenotype. Source: SGD cytokinetic cell separation involved in cell cycle cytokinesis Inferred from mutant phenotype Ref.5. Source: SGD establishment or maintenance of actin cytoskeleton polarity Inferred from mutant phenotype. Source: SGD regulation of fungal-type cell wall organization Inferred from genetic interaction. Source: SGD
Cellular component	cell cortex Inferred from direct assay. Source: SGD cellular bud neck Inferred from direct assay Ref.5. Source: SGD cellular bud tip Inferred from direct assay. Source: SGD incipient cellular bud site Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		3	EBI-4110,EBI-4110
CDC14	Q00684	2	EBI-4110,EBI-4192
MOB2	P43563	5	EBI-4110,EBI-11125
TAO3	P40468	8	EBI-4110,EBI-18961

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 756

756

Serine/threonine-protein kinase CBK1

PRO_0000085697

Regions

Domain

352 – 672

321

Protein kinase

Domain

673 – 754

AGC-kinase C-terminal

Nucleotide binding

358 – 366

ATP By similarity

Compositional bias

23 – 34

Poly-Gln

Compositional bias

160 – 164

Poly-Ser

Compositional bias

214 – 232

Poly-Gln

Compositional bias

235 – 250

Poly-Gln

Sites

Active site

475

Proton acceptor By similarity

Binding site

381

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.7 Ref.8

Modified residue

Phosphoserine Ref.7 Ref.8

Modified residue

109

Phosphothreonine Ref.8

Modified residue

177

Phosphoserine Ref.8

Modified residue

187

Phosphoserine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P53894 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 87EBCD2C3C96EE11
Show »

FASTA

756

86,946

        10         20         30         40         50         60 
MYNSSTNHHE GAPTSGHGYY MSQQQDQQHQ QQQQYANEMN PYQQIPRPPA AGFSSNYMKE 

        70         80         90        100        110        120 
QGSHQSLQEH LQRETGNLGS GFTDVPALNY PATPPPHNNY AASNQMINTP PPSMGGLYRH 

       130        140        150        160        170        180 
NNNSQSMVQN GNGSGNAQLP QLSPGQYSIE SEYNQNLNGS SSSSPFHQPQ TLRSNGSYSS 

       190        200        210        220        230        240 
GLRSVKSFQR LQQEQENVQV QQQLSQAQQQ NSRQQQQQLQ YQQQQQQQQQ QQHMQIQQQQ 

       250        260        270        280        290        300 
QQQQQQQQSQ SPVQSGFNNG TISNYMYFER RPDLLTKGTQ DKAAAVKLKI ENFYQSSVKY 

       310        320        330        340        350        360 
AIERNERRVE LETELTSHNW SEERKSRQLS SLGKKESQFL RLRRTRLSLE DFHTVKVIGK 

       370        380        390        400        410        420 
GAFGEVRLVQ KKDTGKIYAM KTLLKSEMYK KDQLAHVKAE RDVLAGSDSP WVVSLYYSFQ 

       430        440        450        460        470        480 
DAQYLYLIME FLPGGDLMTM LIRWQLFTED VTRFYMAECI LAIETIHKLG FIHRDIKPDN 

       490        500        510        520        530        540 
ILIDIRGHIK LSDFGLSTGF HKTHDSNYYK KLLQQDEATN GISKPGTYNA NTTDTANKRQ 

       550        560        570        580        590        600 
TMVVDSISLT MSNRQQIQTW RKSRRLMAYS TVGTPDYIAP EIFLYQGYGQ ECDWWSLGAI 

       610        620        630        640        650        660 
MYECLIGWPP FCSETPQETY RKIMNFEQTL QFPDDIHISY EAEDLIRRLL THADQRLGRH 

       670        680        690        700        710        720 
GGADEIKSHP FFRGVDWNTI RQVEAPYIPK LSSITDTRFF PTDELENVPD SPAMAQAAKQ 

       730        740        750 
REQMTKQGGS APVKEDLPFI GYTYSRFDYL TRKNAL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase." Nasr F., Becam A.-M., Herbert C.J. Yeast 12:169-175(1996) [PubMed: 8686380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae." Du L.L., Novick P. Mol. Biol. Cell 13:503-514(2002) [PubMed: 11854408] [Abstract] Cited for: INTERACTION WITH PAG1/TAO3.
[5]	"The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor." Weiss E.L., Kurischko C., Zhang C., Shokat K., Drubin D.G., Luca F.C. J. Cell Biol. 158:885-900(2002) [PubMed: 12196508] [Abstract] Cited for: INTERACTION WITH MOB2.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63; SER-66 AND SER-187, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63; SER-66; THR-109 AND SER-177, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92517 Genomic DNA. Translation: CAA63278.1. Z71437 Genomic DNA. Translation: CAA96048.1. BK006947 Genomic DNA. Translation: DAA10388.1.
PIR	S60966.
RefSeq	NP_014238.1. NM_001182999.1.
3D structure databases
ProteinModelPortal	P53894.
SMR	P53894. Positions 339-748.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5656N.
IntAct	P53894. 47 interactions.
MINT	MINT-540797.
STRING	P53894.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL161W; YNL161W; YNL161W.
GeneID	855561.
KEGG	sce:YNL161W.
NMPDR	fig\|4932.3.peg.5304.
Organism-specific databases
CYGD	YNL161w.
SGD	S000005105. CBK1.
Phylogenomic databases
eggNOG	fuNOG04797.
GeneTree	EFGT00070000008712.
HOGENOM	HBG755340.
OMA	IAPEIFV.
OrthoDB	EOG4M3DHS.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
ArrayExpress	P53894.
Genevestigator	P53894.
GermOnline	YNL161W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08286.
Pfam	PF00069. Pkinase. 2 hits. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979655.

Entry information

Entry name

CBK1_YEAST

Accession

Primary (citable) accession number: P53894
Secondary accession number(s): D6W122

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents