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Protein

Serine/threonine-protein kinase CBK1

Gene

CBK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that seems to play a role in the regulation of cell morphogenesis and proliferation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei381 – 3811ATPPROSITE-ProRule annotation
Active sitei475 – 4751Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi358 – 3669ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • budding cell apical bud growth Source: SGD
  • cell separation after cytokinesis Source: SGD
  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein phosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33177-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2028269. Signaling by Hippo.
R-SCE-3928663. EPHA-mediated growth cone collapse.
R-SCE-416482. G alpha (12/13) signalling events.
R-SCE-416572. Sema4D induced cell migration and growth-cone collapse.
R-SCE-4420097. VEGFA-VEGFR2 Pathway.
R-SCE-5625900. RHO GTPases activate CIT.
R-SCE-5627117. RHO GTPases Activate ROCKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase CBK1 (EC:2.7.11.1)
Alternative name(s):
Cell wall biosynthesis kinase
Gene namesi
Name:CBK1
Ordered Locus Names:YNL161W
ORF Names:N1727
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL161W.
SGDiS000005105. CBK1.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: SGD
  • cellular bud Source: SGD
  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 756756Serine/threonine-protein kinase CBK1PRO_0000085697Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53894.

PTM databases

iPTMnetiP53894.

Interactioni

Subunit structurei

Associates with PAG1/TAO3 and interacts with MOB2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-4110,EBI-4110
CDC14Q006842EBI-4110,EBI-4192
MOB2P435633EBI-4110,EBI-11125
TAO3P404687EBI-4110,EBI-18961

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi35668. 120 interactions.
DIPiDIP-5656N.
IntActiP53894. 37 interactions.
MINTiMINT-540797.

Structurei

Secondary structure

1
756
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi299 – 31820Combined sources
Beta strandi349 – 35911Combined sources
Beta strandi366 – 3716Combined sources
Turni372 – 3743Combined sources
Beta strandi378 – 3825Combined sources
Beta strandi385 – 3873Combined sources
Beta strandi415 – 4206Combined sources
Beta strandi425 – 4295Combined sources
Helixi437 – 4437Combined sources
Helixi450 – 4556Combined sources
Helixi458 – 46811Combined sources
Beta strandi478 – 4803Combined sources
Helixi557 – 5604Combined sources
Helixi561 – 5633Combined sources
Beta strandi582 – 5865Combined sources
Helixi591 – 60616Combined sources
Turni619 – 6213Combined sources
Helixi622 – 6254Combined sources
Helixi640 – 64910Combined sources
Beta strandi656 – 6583Combined sources
Beta strandi663 – 6664Combined sources
Helixi679 – 6813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50A251-756[»]
4LQQX-ray3.60A/D251-756[»]
4LQSX-ray3.30A251-756[»]
ProteinModelPortaliP53894.
SMRiP53894. Positions 259-749.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini352 – 672321Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini673 – 75482AGC-kinase C-terminalAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 3412Poly-GlnAdd
BLAST
Compositional biasi160 – 1645Poly-Ser
Compositional biasi214 – 23219Poly-GlnAdd
BLAST
Compositional biasi235 – 25016Poly-GlnAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118994.
HOGENOMiHOG000233033.
InParanoidiP53894.
KOiK08286.
OMAiLAIETIH.
OrthoDBiEOG092C0HH3.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNSSTNHHE GAPTSGHGYY MSQQQDQQHQ QQQQYANEMN PYQQIPRPPA
60 70 80 90 100
AGFSSNYMKE QGSHQSLQEH LQRETGNLGS GFTDVPALNY PATPPPHNNY
110 120 130 140 150
AASNQMINTP PPSMGGLYRH NNNSQSMVQN GNGSGNAQLP QLSPGQYSIE
160 170 180 190 200
SEYNQNLNGS SSSSPFHQPQ TLRSNGSYSS GLRSVKSFQR LQQEQENVQV
210 220 230 240 250
QQQLSQAQQQ NSRQQQQQLQ YQQQQQQQQQ QQHMQIQQQQ QQQQQQQQSQ
260 270 280 290 300
SPVQSGFNNG TISNYMYFER RPDLLTKGTQ DKAAAVKLKI ENFYQSSVKY
310 320 330 340 350
AIERNERRVE LETELTSHNW SEERKSRQLS SLGKKESQFL RLRRTRLSLE
360 370 380 390 400
DFHTVKVIGK GAFGEVRLVQ KKDTGKIYAM KTLLKSEMYK KDQLAHVKAE
410 420 430 440 450
RDVLAGSDSP WVVSLYYSFQ DAQYLYLIME FLPGGDLMTM LIRWQLFTED
460 470 480 490 500
VTRFYMAECI LAIETIHKLG FIHRDIKPDN ILIDIRGHIK LSDFGLSTGF
510 520 530 540 550
HKTHDSNYYK KLLQQDEATN GISKPGTYNA NTTDTANKRQ TMVVDSISLT
560 570 580 590 600
MSNRQQIQTW RKSRRLMAYS TVGTPDYIAP EIFLYQGYGQ ECDWWSLGAI
610 620 630 640 650
MYECLIGWPP FCSETPQETY RKIMNFEQTL QFPDDIHISY EAEDLIRRLL
660 670 680 690 700
THADQRLGRH GGADEIKSHP FFRGVDWNTI RQVEAPYIPK LSSITDTRFF
710 720 730 740 750
PTDELENVPD SPAMAQAAKQ REQMTKQGGS APVKEDLPFI GYTYSRFDYL

TRKNAL
Length:756
Mass (Da):86,946
Last modified:October 1, 1996 - v1
Checksum:i87EBCD2C3C96EE11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63278.1.
Z71437 Genomic DNA. Translation: CAA96048.1.
BK006947 Genomic DNA. Translation: DAA10388.1.
PIRiS60966.
RefSeqiNP_014238.3. NM_001182999.3.

Genome annotation databases

EnsemblFungiiYNL161W; YNL161W; YNL161W.
GeneIDi855561.
KEGGisce:YNL161W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63278.1.
Z71437 Genomic DNA. Translation: CAA96048.1.
BK006947 Genomic DNA. Translation: DAA10388.1.
PIRiS60966.
RefSeqiNP_014238.3. NM_001182999.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50A251-756[»]
4LQQX-ray3.60A/D251-756[»]
4LQSX-ray3.30A251-756[»]
ProteinModelPortaliP53894.
SMRiP53894. Positions 259-749.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35668. 120 interactions.
DIPiDIP-5656N.
IntActiP53894. 37 interactions.
MINTiMINT-540797.

PTM databases

iPTMnetiP53894.

Proteomic databases

MaxQBiP53894.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL161W; YNL161W; YNL161W.
GeneIDi855561.
KEGGisce:YNL161W.

Organism-specific databases

EuPathDBiFungiDB:YNL161W.
SGDiS000005105. CBK1.

Phylogenomic databases

GeneTreeiENSGT00760000118994.
HOGENOMiHOG000233033.
InParanoidiP53894.
KOiK08286.
OMAiLAIETIH.
OrthoDBiEOG092C0HH3.

Enzyme and pathway databases

BioCyciYEAST:G3O-33177-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2028269. Signaling by Hippo.
R-SCE-3928663. EPHA-mediated growth cone collapse.
R-SCE-416482. G alpha (12/13) signalling events.
R-SCE-416572. Sema4D induced cell migration and growth-cone collapse.
R-SCE-4420097. VEGFA-VEGFR2 Pathway.
R-SCE-5625900. RHO GTPases activate CIT.
R-SCE-5627117. RHO GTPases Activate ROCKs.

Miscellaneous databases

PROiP53894.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBK1_YEAST
AccessioniPrimary (citable) accession number: P53894
Secondary accession number(s): D6W122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.