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Protein

Serine/threonine-protein kinase CBK1

Gene

CBK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that seems to play a role in the regulation of cell morphogenesis and proliferation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei381ATPPROSITE-ProRule annotation1
Active sitei475Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi358 – 366ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • budding cell apical bud growth Source: SGD
  • cell separation after cytokinesis Source: SGD
  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein phosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33177-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2028269. Signaling by Hippo.
R-SCE-3928663. EPHA-mediated growth cone collapse.
R-SCE-416482. G alpha (12/13) signalling events.
R-SCE-416572. Sema4D induced cell migration and growth-cone collapse.
R-SCE-4420097. VEGFA-VEGFR2 Pathway.
R-SCE-5627117. RHO GTPases Activate ROCKs.
R-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase CBK1 (EC:2.7.11.1)
Alternative name(s):
Cell wall biosynthesis kinase
Gene namesi
Name:CBK1
Ordered Locus Names:YNL161W
ORF Names:N1727
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL161W.
SGDiS000005105. CBK1.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: SGD
  • cellular bud Source: SGD
  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856971 – 756Serine/threonine-protein kinase CBK1Add BLAST756

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53894.
PRIDEiP53894.

PTM databases

iPTMnetiP53894.

Interactioni

Subunit structurei

Associates with PAG1/TAO3 and interacts with MOB2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-4110,EBI-4110
CDC14Q006842EBI-4110,EBI-4192
MOB2P435633EBI-4110,EBI-11125
TAO3P404687EBI-4110,EBI-18961

Protein-protein interaction databases

BioGridi35668. 120 interactors.
DIPiDIP-5656N.
IntActiP53894. 37 interactors.
MINTiMINT-540797.

Structurei

Secondary structure

1756
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi299 – 318Combined sources20
Beta strandi349 – 359Combined sources11
Beta strandi366 – 371Combined sources6
Turni372 – 374Combined sources3
Beta strandi378 – 382Combined sources5
Beta strandi385 – 387Combined sources3
Beta strandi415 – 420Combined sources6
Beta strandi425 – 429Combined sources5
Helixi437 – 443Combined sources7
Helixi450 – 455Combined sources6
Helixi458 – 468Combined sources11
Beta strandi478 – 480Combined sources3
Helixi557 – 560Combined sources4
Helixi561 – 563Combined sources3
Beta strandi582 – 586Combined sources5
Helixi591 – 606Combined sources16
Turni619 – 621Combined sources3
Helixi622 – 625Combined sources4
Helixi640 – 649Combined sources10
Beta strandi656 – 658Combined sources3
Beta strandi663 – 666Combined sources4
Helixi679 – 681Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50A251-756[»]
4LQQX-ray3.60A/D251-756[»]
4LQSX-ray3.30A251-756[»]
ProteinModelPortaliP53894.
SMRiP53894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini352 – 672Protein kinasePROSITE-ProRule annotationAdd BLAST321
Domaini673 – 754AGC-kinase C-terminalAdd BLAST82

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 34Poly-GlnAdd BLAST12
Compositional biasi160 – 164Poly-Ser5
Compositional biasi214 – 232Poly-GlnAdd BLAST19
Compositional biasi235 – 250Poly-GlnAdd BLAST16

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118994.
HOGENOMiHOG000233033.
InParanoidiP53894.
KOiK08286.
OMAiLAIETIH.
OrthoDBiEOG092C0HH3.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNSSTNHHE GAPTSGHGYY MSQQQDQQHQ QQQQYANEMN PYQQIPRPPA
60 70 80 90 100
AGFSSNYMKE QGSHQSLQEH LQRETGNLGS GFTDVPALNY PATPPPHNNY
110 120 130 140 150
AASNQMINTP PPSMGGLYRH NNNSQSMVQN GNGSGNAQLP QLSPGQYSIE
160 170 180 190 200
SEYNQNLNGS SSSSPFHQPQ TLRSNGSYSS GLRSVKSFQR LQQEQENVQV
210 220 230 240 250
QQQLSQAQQQ NSRQQQQQLQ YQQQQQQQQQ QQHMQIQQQQ QQQQQQQQSQ
260 270 280 290 300
SPVQSGFNNG TISNYMYFER RPDLLTKGTQ DKAAAVKLKI ENFYQSSVKY
310 320 330 340 350
AIERNERRVE LETELTSHNW SEERKSRQLS SLGKKESQFL RLRRTRLSLE
360 370 380 390 400
DFHTVKVIGK GAFGEVRLVQ KKDTGKIYAM KTLLKSEMYK KDQLAHVKAE
410 420 430 440 450
RDVLAGSDSP WVVSLYYSFQ DAQYLYLIME FLPGGDLMTM LIRWQLFTED
460 470 480 490 500
VTRFYMAECI LAIETIHKLG FIHRDIKPDN ILIDIRGHIK LSDFGLSTGF
510 520 530 540 550
HKTHDSNYYK KLLQQDEATN GISKPGTYNA NTTDTANKRQ TMVVDSISLT
560 570 580 590 600
MSNRQQIQTW RKSRRLMAYS TVGTPDYIAP EIFLYQGYGQ ECDWWSLGAI
610 620 630 640 650
MYECLIGWPP FCSETPQETY RKIMNFEQTL QFPDDIHISY EAEDLIRRLL
660 670 680 690 700
THADQRLGRH GGADEIKSHP FFRGVDWNTI RQVEAPYIPK LSSITDTRFF
710 720 730 740 750
PTDELENVPD SPAMAQAAKQ REQMTKQGGS APVKEDLPFI GYTYSRFDYL

TRKNAL
Length:756
Mass (Da):86,946
Last modified:October 1, 1996 - v1
Checksum:i87EBCD2C3C96EE11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63278.1.
Z71437 Genomic DNA. Translation: CAA96048.1.
BK006947 Genomic DNA. Translation: DAA10388.1.
PIRiS60966.
RefSeqiNP_014238.3. NM_001182999.3.

Genome annotation databases

EnsemblFungiiYNL161W; YNL161W; YNL161W.
GeneIDi855561.
KEGGisce:YNL161W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63278.1.
Z71437 Genomic DNA. Translation: CAA96048.1.
BK006947 Genomic DNA. Translation: DAA10388.1.
PIRiS60966.
RefSeqiNP_014238.3. NM_001182999.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50A251-756[»]
4LQQX-ray3.60A/D251-756[»]
4LQSX-ray3.30A251-756[»]
ProteinModelPortaliP53894.
SMRiP53894.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35668. 120 interactors.
DIPiDIP-5656N.
IntActiP53894. 37 interactors.
MINTiMINT-540797.

PTM databases

iPTMnetiP53894.

Proteomic databases

MaxQBiP53894.
PRIDEiP53894.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL161W; YNL161W; YNL161W.
GeneIDi855561.
KEGGisce:YNL161W.

Organism-specific databases

EuPathDBiFungiDB:YNL161W.
SGDiS000005105. CBK1.

Phylogenomic databases

GeneTreeiENSGT00760000118994.
HOGENOMiHOG000233033.
InParanoidiP53894.
KOiK08286.
OMAiLAIETIH.
OrthoDBiEOG092C0HH3.

Enzyme and pathway databases

BioCyciYEAST:G3O-33177-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2028269. Signaling by Hippo.
R-SCE-3928663. EPHA-mediated growth cone collapse.
R-SCE-416482. G alpha (12/13) signalling events.
R-SCE-416572. Sema4D induced cell migration and growth-cone collapse.
R-SCE-4420097. VEGFA-VEGFR2 Pathway.
R-SCE-5627117. RHO GTPases Activate ROCKs.
R-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP53894.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBK1_YEAST
AccessioniPrimary (citable) accession number: P53894
Secondary accession number(s): D6W122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.