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Protein

Ribosome assembly protein 1

Gene

RIA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SDO1, may trigger the GTP-dependent release of TIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating TIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Inhibits GTPase activity of ribosome-bound EF-2.3 Publications

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.

Enzyme regulationi

GTPase activity is stimulated in the presence of 60S subunits.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33GTPBy similarity8
Nucleotide bindingi102 – 106GTPBy similarity5
Nucleotide bindingi156 – 159GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • mature ribosome assembly Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processRibosome biogenesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33179-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome assembly protein 1 (EC:3.6.5.-)
Alternative name(s):
EF-2-like GTPase
Elongation factor-like 1
Gene namesi
Name:RIA1
Synonyms:EFL1
Ordered Locus Names:YNL163C
ORF Names:N1718
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL163C.
SGDiS000005107. RIA1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915591 – 1110Ribosome assembly protein 1Add BLAST1110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei431PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53893.
PRIDEiP53893.

PTM databases

iPTMnetiP53893.

Interactioni

Protein-protein interaction databases

BioGridi35665. 360 interactors.
DIPiDIP-2553N.
IntActiP53893. 4 interactors.
MINTiMINT-424547.
STRINGi4932.YNL163C.

Structurei

3D structure databases

ProteinModelPortaliP53893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 262tr-type GPROSITE-ProRule annotationAdd BLAST246

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074806.
HOGENOMiHOG000231586.
InParanoidiP53893.
KOiK14536.
OMAiADACVQV.
OrthoDBiEOG092C0D4V.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
InterProiView protein in InterPro
IPR035647. EFG_III/V.
IPR000640. EFG_V-like.
IPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
PfamiView protein in Pfam
PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SMARTiView protein in SMART
SM00838. EFG_C. 1 hit.
SUPFAMiSSF50447. SSF50447. 3 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P53893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRVESETYK RLQNDPSCIR NICIVAHVDH GKTSLSDSLL ASNGIISQRL
60 70 80 90 100
AGKIRFLDAR PDEQLRGITM ESSAISLYFR VLRKQEGSDE PLVSEHLVNL
110 120 130 140 150
IDSPGHIDFS SEVSAASRLC DGAVVLVDVV EGVCSQTVTV LRQCWTEKLK
160 170 180 190 200
PILVLNKIDR LITELQLTPQ EAYIHLSKVI EQVNSVIGSF FANERQLDDL
210 220 230 240 250
FWREQLEKNE NAEYIEKDDS GIYFNPTDNN VIFASAIDGW GFNIGQLAKF
260 270 280 290 300
YEQKLGAKRE NLQKVLWGDF YMDPKTKKII NNKGLKGRSL KPLFTSLILE
310 320 330 340 350
NIWKIYQNII TSRDSEMVEK IAKTLNIKLL ARDLRSKDDK QLLRTIMGQW
360 370 380 390 400
LPVSTAVLLT VIEKLPSPLE SQTDRLNTIL VSESDTAAMD PRLLKAMKTC
410 420 430 440 450
DKEGPVSAYV SKMLSIPREE LPVESKRIAS SDELMERSRK AREEALNAAK
460 470 480 490 500
HAGMVENMAM MDLNDNSKNT SDLYKRAKDT VMTPEVGEQT KPKPSRNNDV
510 520 530 540 550
FCVVSEPSSA LDLEFEYEGE DDSDSQDNFG LDFVPTDIDP NDPLSSMFEY
560 570 580 590 600
EEEDPLLESI KQISEDVNDE VDDIFDEKEE CLVAFARIYS GTLRVGQEIS
610 620 630 640 650
VLGPKYDPKC PEEHIETAII THLYLFMGKE LVPLDVCPSG NIVGIRGLAG
660 670 680 690 700
KVLKSGTLIE KGVQGVNLAG VNFHFTPIVR VAVEPANPVE MSKLVRGLKL
710 720 730 740 750
LDQADPCVHT YVENTGEHIL CTAGELHLER CLKDLTERFA GIEITHSEPA
760 770 780 790 800
IPYRETFLSA SDMNPPQNSQ LGRGVHELLL SQYKITFRTF PLSGKVTDFL
810 820 830 840 850
SQHQNSIKNI LKTSTSSMDP VIESTGSSFL DKKSLLVAFE EVINQEEKSR
860 870 880 890 900
ELLSGFKVKL AGFGPSRVGC NILLSQDNLL GSLFEGTPAA FEYSDSIKNG
910 920 930 940 950
FQLAVSEGPL ANEPVQGMCV LVESVHKMSQ DEIESIEDPR YQQHIVDLSG
960 970 980 990 1000
RLITSTRDAI HEAFLDWSPR IMWAIYSCDI QTSVDVLGKV YAVILQRHGK
1010 1020 1030 1040 1050
IISEEMKEGT PFFQIEAHVP VVEAFGLSED IRKRTSGAAQ PQLVFSGFEC
1060 1070 1080 1090 1100
IDLDPFWVPT TEEELEELGD TADRENIARK HMNAIRRRKG LFIEEKVVEN
1110
AEKQRTLKKN
Length:1,110
Mass (Da):124,464
Last modified:October 1, 1996 - v1
Checksum:i6FFD6ACD53F4510C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92517 Genomic DNA. Translation: CAA63276.1.
Z71439 Genomic DNA. Translation: CAA96050.1.
BK006947 Genomic DNA. Translation: DAA10385.1.
PIRiS60964.
RefSeqiNP_014236.1. NM_001183001.1.

Genome annotation databases

EnsemblFungiiYNL163C; YNL163C; YNL163C.
GeneIDi855558.
KEGGisce:YNL163C.

Similar proteinsi

Entry informationi

Entry nameiRIA1_YEAST
AccessioniPrimary (citable) accession number: P53893
Secondary accession number(s): D6W119
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names