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P53893 (RIA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome assembly protein 1

EC=3.6.5.-
Alternative name(s):
EF-2-like GTPase
Elongation factor-like 1
Gene names
Name:RIA1
Synonyms:EFL1
Ordered Locus Names:YNL163C
ORF Names:N1718
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1110 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SDO1, may trigger the GTP-dependent release of TIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating TIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Inhibits GTPase activity of ribosome-bound EF-2. Ref.4 Ref.5 Ref.8

Catalytic activity

GTP + H2O = GDP + phosphate.

Enzyme regulation

GTPase activity is stimulated in the presence of 60S subunits. Ref.4

Subcellular location

Cytoplasm Ref.4 Ref.5 Ref.6.

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GTP-binding elongation factor family.

Ontologies

Keywords
   Biological processRibosome biogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay Ref.4. Source: GOC

mature ribosome assembly

Inferred from mutant phenotype Ref.4. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay Ref.4. Source: SGD

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from direct assay Ref.4. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11101110Ribosome assembly protein 1
PRO_0000091559

Regions

Nucleotide binding26 – 338GTP By similarity
Nucleotide binding102 – 1065GTP By similarity
Nucleotide binding156 – 1594GTP By similarity

Amino acid modifications

Modified residue4311Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P53893 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6FFD6ACD53F4510C

FASTA1,110124,464
        10         20         30         40         50         60 
MPRVESETYK RLQNDPSCIR NICIVAHVDH GKTSLSDSLL ASNGIISQRL AGKIRFLDAR 

        70         80         90        100        110        120 
PDEQLRGITM ESSAISLYFR VLRKQEGSDE PLVSEHLVNL IDSPGHIDFS SEVSAASRLC 

       130        140        150        160        170        180 
DGAVVLVDVV EGVCSQTVTV LRQCWTEKLK PILVLNKIDR LITELQLTPQ EAYIHLSKVI 

       190        200        210        220        230        240 
EQVNSVIGSF FANERQLDDL FWREQLEKNE NAEYIEKDDS GIYFNPTDNN VIFASAIDGW 

       250        260        270        280        290        300 
GFNIGQLAKF YEQKLGAKRE NLQKVLWGDF YMDPKTKKII NNKGLKGRSL KPLFTSLILE 

       310        320        330        340        350        360 
NIWKIYQNII TSRDSEMVEK IAKTLNIKLL ARDLRSKDDK QLLRTIMGQW LPVSTAVLLT 

       370        380        390        400        410        420 
VIEKLPSPLE SQTDRLNTIL VSESDTAAMD PRLLKAMKTC DKEGPVSAYV SKMLSIPREE 

       430        440        450        460        470        480 
LPVESKRIAS SDELMERSRK AREEALNAAK HAGMVENMAM MDLNDNSKNT SDLYKRAKDT 

       490        500        510        520        530        540 
VMTPEVGEQT KPKPSRNNDV FCVVSEPSSA LDLEFEYEGE DDSDSQDNFG LDFVPTDIDP 

       550        560        570        580        590        600 
NDPLSSMFEY EEEDPLLESI KQISEDVNDE VDDIFDEKEE CLVAFARIYS GTLRVGQEIS 

       610        620        630        640        650        660 
VLGPKYDPKC PEEHIETAII THLYLFMGKE LVPLDVCPSG NIVGIRGLAG KVLKSGTLIE 

       670        680        690        700        710        720 
KGVQGVNLAG VNFHFTPIVR VAVEPANPVE MSKLVRGLKL LDQADPCVHT YVENTGEHIL 

       730        740        750        760        770        780 
CTAGELHLER CLKDLTERFA GIEITHSEPA IPYRETFLSA SDMNPPQNSQ LGRGVHELLL 

       790        800        810        820        830        840 
SQYKITFRTF PLSGKVTDFL SQHQNSIKNI LKTSTSSMDP VIESTGSSFL DKKSLLVAFE 

       850        860        870        880        890        900 
EVINQEEKSR ELLSGFKVKL AGFGPSRVGC NILLSQDNLL GSLFEGTPAA FEYSDSIKNG 

       910        920        930        940        950        960 
FQLAVSEGPL ANEPVQGMCV LVESVHKMSQ DEIESIEDPR YQQHIVDLSG RLITSTRDAI 

       970        980        990       1000       1010       1020 
HEAFLDWSPR IMWAIYSCDI QTSVDVLGKV YAVILQRHGK IISEEMKEGT PFFQIEAHVP 

      1030       1040       1050       1060       1070       1080 
VVEAFGLSED IRKRTSGAAQ PQLVFSGFEC IDLDPFWVPT TEEELEELGD TADRENIARK 

      1090       1100       1110 
HMNAIRRRKG LFIEEKVVEN AEKQRTLKKN 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis."
Senger B., Lafontaine D.L.J., Graindorge J.-S., Gadal O., Camasses A., Sanni A., Garnier J.-M., Breitenbach M., Hurt E., Fasiolo F.
Mol. Cell 8:1363-1373(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
[5]"Ria1p (Ynl163c), a protein similar to elongation factors 2, is involved in the biogenesis of the 60S subunit of the ribosome in Saccharomyces cerevisiae."
Becam A.-M., Nasr F., Racki W.J., Zagulski M., Herbert C.J.
Mol. Genet. Genomics 266:454-462(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Deletion of EFL1 results in heterogeneity of the 60 S GTPase-associated rRNA conformation."
Graindorge J.-S., Rousselle J.-C., Senger B., Lenormand P., Namane A., Lacroute F., Fasiolo F.
J. Mol. Biol. 352:355-369(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92517 Genomic DNA. Translation: CAA63276.1.
Z71439 Genomic DNA. Translation: CAA96050.1.
BK006947 Genomic DNA. Translation: DAA10385.1.
PIRS60964.
RefSeqNP_014236.1. NM_001183001.1.

3D structure databases

ProteinModelPortalP53893.
SMRP53893. Positions 19-414, 584-1056.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35665. 219 interactions.
DIPDIP-2553N.
IntActP53893. 3 interactions.
MINTMINT-424547.

Proteomic databases

PaxDbP53893.
PeptideAtlasP53893.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL163C; YNL163C; YNL163C.
GeneID855558.
KEGGsce:YNL163C.

Organism-specific databases

CYGDYNL163c.
SGDS000005107. RIA1.

Phylogenomic databases

eggNOGCOG0480.
GeneTreeENSGT00550000074806.
HOGENOMHOG000231586.
KOK14536.
OMAMYTCDIQ.
OrthoDBEOG7PVWXS.

Enzyme and pathway databases

BioCycYEAST:G3O-33179-MONOMER.

Gene expression databases

GenevestigatorP53893.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 3 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Other

NextBio979646.
PROP53893.

Entry information

Entry nameRIA1_YEAST
AccessionPrimary (citable) accession number: P53893
Secondary accession number(s): D6W119
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families