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P53886 (APC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaphase-promoting complex subunit 1
Gene names
Name:APC1
Ordered Locus Names:YNL172W
ORF Names:N1677
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1748 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. Ref.3 Ref.4

Pathway

Protein modification; protein ubiquitination.

Subunit structure

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC1 interacts directly with MND2. Ref.3 Ref.4

Subcellular location

Nucleus. Cytoplasm. Cytoplasmcytoskeletonspindle pole Ref.5.

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the APC1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MND2P405773EBI-29017,EBI-25433

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17481748Anaphase-promoting complex subunit 1
PRO_0000215875

Regions

Compositional bias353 – 3575Poly-Gln

Amino acid modifications

Modified residue14621Phosphoserine Ref.8

Experimental info

Sequence conflict15471M → I in CAA96060. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53886 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 33D32EC47CA40E61

FASTA1,748196,162
        10         20         30         40         50         60 
MTSKPSTRND YLPRETHNGE YTGDSPEWQL QINITNKIGG INGDIWLSRD GRSVKWCIED 

        70         80         90        100        110        120 
QCLRQFTYNQ KIIKAGYIDF EKTPDCFVVV LSDIAHVYML KNGGSTTVCF PFQIGNAFWY 

       130        140        150        160        170        180 
ANGVILERET SASFMDGGYD LKPIEFDLKH KYITLTDPMA PFGLISITNT FKGGINSASG 

       190        200        210        220        230        240 
NKTDILQDFQ LVLFPSDKDK CIAVFLDRNS KVLRFYYSRI LSSDQSRKGE LTISSTKKTG 

       250        260        270        280        290        300 
LDAAGNSQKS GGISKDLRKF SHLTRRSTSI NSNSHDFNAA ERVLSGNVGN ASGRTDIFAL 

       310        320        330        340        350        360 
PSSCSRRSLS ATLDRMGNNI APTNRAAPSG FFDSSSANTA THSNITPVSQ PMQQQQQEYL 

       370        380        390        400        410        420 
NQTATSSKDI VLTEISSLKL PDDIIFTSRR LSSILSKLKF LSLRFERREG LLIFHEPTHF 

       430        440        450        460        470        480 
CKIWLIDLLP DVLDSIPFKI YGNSPQNMIR LENLKLKEPS RIQAMYIHEL LESCLILVSE 

       490        500        510        520        530        540 
GQNKEEYKAC LYDPFVKITS PSKNISEELT KQNSLPSLQK LFPYPETSFT KLCFEAVKYI 

       550        560        570        580        590        600 
TSPAFNISFI FLWQSAYSIL LSRANDDVVG GLKMEHDAFS LVLSLLILPI PSSSAQEYQE 

       610        620        630        640        650        660 
YKEIYERDLF QHLKQDSEIT SSVLPRIVIG LHLIREEYSL NVLCRNEHAL LGQFLRFATA 

       670        680        690        700        710        720 
AMGWPDLWQS YYVPKMDSES KTFLHPREQN STFFHPLDEP PSITKSLYSI TENSSIPLCP 

       730        740        750        760        770        780 
FISFSRLVAT DTQVELRITP RSFKILGLYE LVHSPNFLPD YVLGILSSFK VDKDELQTYP 

       790        800        810        820        830        840 
LGILVPLQNI LKILEDKLSE VRDNLELLDR ADLQRCSAII NSIRSDSKEV LKRGQRDSYM 

       850        860        870        880        890        900 
LCKVPLAKNR SSLSKKPSDI YSILSEIVKS ASQVPLDGSA MRMSNIQDDE DIDEGRSLKL 

       910        920        930        940        950        960 
NAGLIFSEDK RFTHVVSLLA YYRPTKTQFF TTKTEYAQIL AQKKYFAKIM ALRTCTNGVG 

       970        980        990       1000       1010       1020 
WGAVAYATEK PISTQKWVIQ PLNLISVFPD DTKITVKAPE DIAHDIVEWG QFHAGVSSGL 

      1030       1040       1050       1060       1070       1080 
RISKKATGIT GSWIAFNKPK ELDAYHGGFL LGLGLNGHLK NLEEWHIYNY LSPRNTHISI 

      1090       1100       1110       1120       1130       1140 
GLLLGMSSSM KGSMDSKLIK VISVHLVAFL PSGSSDLNID LKLQTAGIIG MGMLYLNSRH 

      1150       1160       1170       1180       1190       1200 
KRMSDSIFAQ LVSLLNVNDE MVADEEYRLA AGISLGLINL GAGQTKLRKW DSSLLGLGDD 

      1210       1220       1230       1240       1250       1260 
LPEDVYDSSD VEQNVMYEDL TTKLLEIVTS TYDVENDWIP ENSQIGAVIA IMFLFLKSNN 

      1270       1280       1290       1300       1310       1320 
FGISNMLKVD LKEILKANIN TRPELLMYRE WASNMILWEF IGDDLSFIMK DVDIGVKFSE 

      1330       1340       1350       1360       1370       1380 
LNTDLLPIYY TMAGRILAMG IRFASTGNLK IRNILLSLVD KFLPLYQYPG KQNLDFRLTI 

      1390       1400       1410       1420       1430       1440 
SVINVLTNVI VVSLSMVMCA SGDLEVLRRV KYLHEVASGP YSDLFQEIPS SKSDVSGVTQ 

      1450       1460       1470       1480       1490       1500 
VTSNTNTPGN SDRERVDETA ASLDDERSSN GSDISDPTAY LEDKKDIDDH YGKFISTNLA 

      1510       1520       1530       1540       1550       1560 
LGFLFLGSGQ YALNTSTLES IAFLSMSVLP TYTTPHPLQE LKHFWSMAVE PRCLVIKDIS 

      1570       1580       1590       1600       1610       1620 
TGDAVNNVPI ELVVEEDVEK EEVIREISTP CLLPDFSKIK SIRVKMHGYF PLEVNFTKDY 

      1630       1640       1650       1660       1670       1680 
SASDFFSGGT IIYIQRKSES VFENKASFRN VEDIHVALKR KAAESKNYSR LNLKNEQGNT 

      1690       1700       1710       1720       1730       1740 
TSSQLVESLG IQDLTMVELD TLLSAGNNTA LTDSESYNLG LLCSDKNSGD ILDCQLELWY 


KSFGPHDE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 1547.
Strain: ATCC 204508 / S288c.
[3]"Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae."
Zachariae W., Shin T.H., Galova M., Obermaier B., Nasmyth K.
Science 274:1201-1204(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[4]"Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex."
Hall M.C., Torres M.P., Schroeder G.K., Borchers C.H.
J. Biol. Chem. 278:16698-16705(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MND2.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71448 Genomic DNA. Translation: CAA96060.1.
BK006947 Genomic DNA. Translation: DAA10378.2.
PIRS63127.
RefSeqNP_014227.4. NM_001183010.4.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35658. 50 interactions.
DIPDIP-970N.
IntActP53886. 13 interactions.
MINTMINT-633685.
STRING4932.YNL172W.

Proteomic databases

MaxQBP53886.
PaxDbP53886.
PeptideAtlasP53886.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL172W; YNL172W; YNL172W.
GeneID855549.
KEGGsce:YNL172W.

Organism-specific databases

CYGDYNL172w.
SGDS000005116. APC1.

Phylogenomic databases

eggNOGNOG316940.
GeneTreeENSGT00390000016757.
HOGENOMHOG000033985.
KOK03348.
OMAMYREWAS.
OrthoDBEOG7NW6JC.

Enzyme and pathway databases

BioCycYEAST:G3O-33185-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP53886.

Family and domain databases

InterProIPR024990. Apc1.
[Graphical view]
PANTHERPTHR12827. PTHR12827. 1 hit.
PfamPF12859. Apc1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979625.

Entry information

Entry nameAPC1_YEAST
AccessionPrimary (citable) accession number: P53886
Secondary accession number(s): D6W112
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways