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P53886

- APC1_YEAST

UniProt

P53886 - APC1_YEAST

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Protein
Anaphase-promoting complex subunit 1
Gene
APC1, YNL172W, N1677
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.2 Publications

Pathwayi

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  2. cyclin catabolic process Source: SGD
  3. exit from mitosis Source: SGD
  4. metaphase/anaphase transition of mitotic cell cycle Source: SGD
  5. protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33185-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 1
Gene namesi
Name:APC1
Ordered Locus Names:YNL172W
ORF Names:N1677
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL172w.
SGDiS000005116. APC1.

Subcellular locationi

Nucleus. Cytoplasm. Cytoplasmcytoskeletonspindle pole 1 Publication

GO - Cellular componenti

  1. anaphase-promoting complex Source: SGD
  2. cytoplasm Source: UniProtKB-SubCell
  3. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17481748Anaphase-promoting complex subunit 1
PRO_0000215875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1462 – 14621Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53886.
PaxDbiP53886.
PeptideAtlasiP53886.

Expressioni

Gene expression databases

GenevestigatoriP53886.

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC1 interacts directly with MND2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MND2P405773EBI-29017,EBI-25433

Protein-protein interaction databases

BioGridi35658. 50 interactions.
DIPiDIP-970N.
IntActiP53886. 13 interactions.
MINTiMINT-633685.
STRINGi4932.YNL172W.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi353 – 3575Poly-Gln

Sequence similaritiesi

Belongs to the APC1 family.

Phylogenomic databases

eggNOGiNOG316940.
GeneTreeiENSGT00390000016757.
HOGENOMiHOG000033985.
KOiK03348.
OMAiMYREWAS.
OrthoDBiEOG7NW6JC.

Family and domain databases

InterProiIPR024990. Apc1.
[Graphical view]
PANTHERiPTHR12827. PTHR12827. 1 hit.
PfamiPF12859. Apc1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53886-1 [UniParc]FASTAAdd to Basket

« Hide

MTSKPSTRND YLPRETHNGE YTGDSPEWQL QINITNKIGG INGDIWLSRD     50
GRSVKWCIED QCLRQFTYNQ KIIKAGYIDF EKTPDCFVVV LSDIAHVYML 100
KNGGSTTVCF PFQIGNAFWY ANGVILERET SASFMDGGYD LKPIEFDLKH 150
KYITLTDPMA PFGLISITNT FKGGINSASG NKTDILQDFQ LVLFPSDKDK 200
CIAVFLDRNS KVLRFYYSRI LSSDQSRKGE LTISSTKKTG LDAAGNSQKS 250
GGISKDLRKF SHLTRRSTSI NSNSHDFNAA ERVLSGNVGN ASGRTDIFAL 300
PSSCSRRSLS ATLDRMGNNI APTNRAAPSG FFDSSSANTA THSNITPVSQ 350
PMQQQQQEYL NQTATSSKDI VLTEISSLKL PDDIIFTSRR LSSILSKLKF 400
LSLRFERREG LLIFHEPTHF CKIWLIDLLP DVLDSIPFKI YGNSPQNMIR 450
LENLKLKEPS RIQAMYIHEL LESCLILVSE GQNKEEYKAC LYDPFVKITS 500
PSKNISEELT KQNSLPSLQK LFPYPETSFT KLCFEAVKYI TSPAFNISFI 550
FLWQSAYSIL LSRANDDVVG GLKMEHDAFS LVLSLLILPI PSSSAQEYQE 600
YKEIYERDLF QHLKQDSEIT SSVLPRIVIG LHLIREEYSL NVLCRNEHAL 650
LGQFLRFATA AMGWPDLWQS YYVPKMDSES KTFLHPREQN STFFHPLDEP 700
PSITKSLYSI TENSSIPLCP FISFSRLVAT DTQVELRITP RSFKILGLYE 750
LVHSPNFLPD YVLGILSSFK VDKDELQTYP LGILVPLQNI LKILEDKLSE 800
VRDNLELLDR ADLQRCSAII NSIRSDSKEV LKRGQRDSYM LCKVPLAKNR 850
SSLSKKPSDI YSILSEIVKS ASQVPLDGSA MRMSNIQDDE DIDEGRSLKL 900
NAGLIFSEDK RFTHVVSLLA YYRPTKTQFF TTKTEYAQIL AQKKYFAKIM 950
ALRTCTNGVG WGAVAYATEK PISTQKWVIQ PLNLISVFPD DTKITVKAPE 1000
DIAHDIVEWG QFHAGVSSGL RISKKATGIT GSWIAFNKPK ELDAYHGGFL 1050
LGLGLNGHLK NLEEWHIYNY LSPRNTHISI GLLLGMSSSM KGSMDSKLIK 1100
VISVHLVAFL PSGSSDLNID LKLQTAGIIG MGMLYLNSRH KRMSDSIFAQ 1150
LVSLLNVNDE MVADEEYRLA AGISLGLINL GAGQTKLRKW DSSLLGLGDD 1200
LPEDVYDSSD VEQNVMYEDL TTKLLEIVTS TYDVENDWIP ENSQIGAVIA 1250
IMFLFLKSNN FGISNMLKVD LKEILKANIN TRPELLMYRE WASNMILWEF 1300
IGDDLSFIMK DVDIGVKFSE LNTDLLPIYY TMAGRILAMG IRFASTGNLK 1350
IRNILLSLVD KFLPLYQYPG KQNLDFRLTI SVINVLTNVI VVSLSMVMCA 1400
SGDLEVLRRV KYLHEVASGP YSDLFQEIPS SKSDVSGVTQ VTSNTNTPGN 1450
SDRERVDETA ASLDDERSSN GSDISDPTAY LEDKKDIDDH YGKFISTNLA 1500
LGFLFLGSGQ YALNTSTLES IAFLSMSVLP TYTTPHPLQE LKHFWSMAVE 1550
PRCLVIKDIS TGDAVNNVPI ELVVEEDVEK EEVIREISTP CLLPDFSKIK 1600
SIRVKMHGYF PLEVNFTKDY SASDFFSGGT IIYIQRKSES VFENKASFRN 1650
VEDIHVALKR KAAESKNYSR LNLKNEQGNT TSSQLVESLG IQDLTMVELD 1700
TLLSAGNNTA LTDSESYNLG LLCSDKNSGD ILDCQLELWY KSFGPHDE 1748
Length:1,748
Mass (Da):196,162
Last modified:September 21, 2011 - v2
Checksum:i33D32EC47CA40E61
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1547 – 15471M → I in CAA96060. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71448 Genomic DNA. Translation: CAA96060.1.
BK006947 Genomic DNA. Translation: DAA10378.2.
PIRiS63127.
RefSeqiNP_014227.4. NM_001183010.4.

Genome annotation databases

EnsemblFungiiYNL172W; YNL172W; YNL172W.
GeneIDi855549.
KEGGisce:YNL172W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71448 Genomic DNA. Translation: CAA96060.1 .
BK006947 Genomic DNA. Translation: DAA10378.2 .
PIRi S63127.
RefSeqi NP_014227.4. NM_001183010.4.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

BioGridi 35658. 50 interactions.
DIPi DIP-970N.
IntActi P53886. 13 interactions.
MINTi MINT-633685.
STRINGi 4932.YNL172W.

Proteomic databases

MaxQBi P53886.
PaxDbi P53886.
PeptideAtlasi P53886.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL172W ; YNL172W ; YNL172W .
GeneIDi 855549.
KEGGi sce:YNL172W.

Organism-specific databases

CYGDi YNL172w.
SGDi S000005116. APC1.

Phylogenomic databases

eggNOGi NOG316940.
GeneTreei ENSGT00390000016757.
HOGENOMi HOG000033985.
KOi K03348.
OMAi MYREWAS.
OrthoDBi EOG7NW6JC.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-33185-MONOMER.

Miscellaneous databases

NextBioi 979625.

Gene expression databases

Genevestigatori P53886.

Family and domain databases

InterProi IPR024990. Apc1.
[Graphical view ]
PANTHERi PTHR12827. PTHR12827. 1 hit.
Pfami PF12859. Apc1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 1547.
    Strain: ATCC 204508 / S288c.
  3. "Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae."
    Zachariae W., Shin T.H., Galova M., Obermaier B., Nasmyth K.
    Science 274:1201-1204(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  4. "Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex."
    Hall M.C., Torres M.P., Schroeder G.K., Borchers C.H.
    J. Biol. Chem. 278:16698-16705(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MND2.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAPC1_YEAST
AccessioniPrimary (citable) accession number: P53886
Secondary accession number(s): D6W112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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