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P53886

- APC1_YEAST

UniProt

P53886 - APC1_YEAST

Protein

Anaphase-promoting complex subunit 1

Gene

APC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
    2. cyclin catabolic process Source: SGD
    3. exit from mitosis Source: SGD
    4. metaphase/anaphase transition of mitotic cell cycle Source: SGD
    5. protein ubiquitination Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33185-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaphase-promoting complex subunit 1
    Gene namesi
    Name:APC1
    Ordered Locus Names:YNL172W
    ORF Names:N1677
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL172w.
    SGDiS000005116. APC1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Cytoplasmcytoskeletonspindle pole 1 Publication

    GO - Cellular componenti

    1. anaphase-promoting complex Source: SGD
    2. cytoplasm Source: UniProtKB-SubCell
    3. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17481748Anaphase-promoting complex subunit 1PRO_0000215875Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1462 – 14621Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53886.
    PaxDbiP53886.
    PeptideAtlasiP53886.

    Expressioni

    Gene expression databases

    GenevestigatoriP53886.

    Interactioni

    Subunit structurei

    The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC1 interacts directly with MND2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MND2P405773EBI-29017,EBI-25433

    Protein-protein interaction databases

    BioGridi35658. 50 interactions.
    DIPiDIP-970N.
    IntActiP53886. 13 interactions.
    MINTiMINT-633685.
    STRINGi4932.YNL172W.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi353 – 3575Poly-Gln

    Sequence similaritiesi

    Belongs to the APC1 family.Curated

    Phylogenomic databases

    eggNOGiNOG316940.
    GeneTreeiENSGT00390000016757.
    HOGENOMiHOG000033985.
    KOiK03348.
    OMAiMYREWAS.
    OrthoDBiEOG7NW6JC.

    Family and domain databases

    InterProiIPR024990. Apc1.
    [Graphical view]
    PANTHERiPTHR12827. PTHR12827. 1 hit.
    PfamiPF12859. Apc1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53886-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSKPSTRND YLPRETHNGE YTGDSPEWQL QINITNKIGG INGDIWLSRD     50
    GRSVKWCIED QCLRQFTYNQ KIIKAGYIDF EKTPDCFVVV LSDIAHVYML 100
    KNGGSTTVCF PFQIGNAFWY ANGVILERET SASFMDGGYD LKPIEFDLKH 150
    KYITLTDPMA PFGLISITNT FKGGINSASG NKTDILQDFQ LVLFPSDKDK 200
    CIAVFLDRNS KVLRFYYSRI LSSDQSRKGE LTISSTKKTG LDAAGNSQKS 250
    GGISKDLRKF SHLTRRSTSI NSNSHDFNAA ERVLSGNVGN ASGRTDIFAL 300
    PSSCSRRSLS ATLDRMGNNI APTNRAAPSG FFDSSSANTA THSNITPVSQ 350
    PMQQQQQEYL NQTATSSKDI VLTEISSLKL PDDIIFTSRR LSSILSKLKF 400
    LSLRFERREG LLIFHEPTHF CKIWLIDLLP DVLDSIPFKI YGNSPQNMIR 450
    LENLKLKEPS RIQAMYIHEL LESCLILVSE GQNKEEYKAC LYDPFVKITS 500
    PSKNISEELT KQNSLPSLQK LFPYPETSFT KLCFEAVKYI TSPAFNISFI 550
    FLWQSAYSIL LSRANDDVVG GLKMEHDAFS LVLSLLILPI PSSSAQEYQE 600
    YKEIYERDLF QHLKQDSEIT SSVLPRIVIG LHLIREEYSL NVLCRNEHAL 650
    LGQFLRFATA AMGWPDLWQS YYVPKMDSES KTFLHPREQN STFFHPLDEP 700
    PSITKSLYSI TENSSIPLCP FISFSRLVAT DTQVELRITP RSFKILGLYE 750
    LVHSPNFLPD YVLGILSSFK VDKDELQTYP LGILVPLQNI LKILEDKLSE 800
    VRDNLELLDR ADLQRCSAII NSIRSDSKEV LKRGQRDSYM LCKVPLAKNR 850
    SSLSKKPSDI YSILSEIVKS ASQVPLDGSA MRMSNIQDDE DIDEGRSLKL 900
    NAGLIFSEDK RFTHVVSLLA YYRPTKTQFF TTKTEYAQIL AQKKYFAKIM 950
    ALRTCTNGVG WGAVAYATEK PISTQKWVIQ PLNLISVFPD DTKITVKAPE 1000
    DIAHDIVEWG QFHAGVSSGL RISKKATGIT GSWIAFNKPK ELDAYHGGFL 1050
    LGLGLNGHLK NLEEWHIYNY LSPRNTHISI GLLLGMSSSM KGSMDSKLIK 1100
    VISVHLVAFL PSGSSDLNID LKLQTAGIIG MGMLYLNSRH KRMSDSIFAQ 1150
    LVSLLNVNDE MVADEEYRLA AGISLGLINL GAGQTKLRKW DSSLLGLGDD 1200
    LPEDVYDSSD VEQNVMYEDL TTKLLEIVTS TYDVENDWIP ENSQIGAVIA 1250
    IMFLFLKSNN FGISNMLKVD LKEILKANIN TRPELLMYRE WASNMILWEF 1300
    IGDDLSFIMK DVDIGVKFSE LNTDLLPIYY TMAGRILAMG IRFASTGNLK 1350
    IRNILLSLVD KFLPLYQYPG KQNLDFRLTI SVINVLTNVI VVSLSMVMCA 1400
    SGDLEVLRRV KYLHEVASGP YSDLFQEIPS SKSDVSGVTQ VTSNTNTPGN 1450
    SDRERVDETA ASLDDERSSN GSDISDPTAY LEDKKDIDDH YGKFISTNLA 1500
    LGFLFLGSGQ YALNTSTLES IAFLSMSVLP TYTTPHPLQE LKHFWSMAVE 1550
    PRCLVIKDIS TGDAVNNVPI ELVVEEDVEK EEVIREISTP CLLPDFSKIK 1600
    SIRVKMHGYF PLEVNFTKDY SASDFFSGGT IIYIQRKSES VFENKASFRN 1650
    VEDIHVALKR KAAESKNYSR LNLKNEQGNT TSSQLVESLG IQDLTMVELD 1700
    TLLSAGNNTA LTDSESYNLG LLCSDKNSGD ILDCQLELWY KSFGPHDE 1748
    Length:1,748
    Mass (Da):196,162
    Last modified:September 21, 2011 - v2
    Checksum:i33D32EC47CA40E61
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1547 – 15471M → I in CAA96060. (PubMed:9169873)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71448 Genomic DNA. Translation: CAA96060.1.
    BK006947 Genomic DNA. Translation: DAA10378.2.
    PIRiS63127.
    RefSeqiNP_014227.4. NM_001183010.4.

    Genome annotation databases

    EnsemblFungiiYNL172W; YNL172W; YNL172W.
    GeneIDi855549.
    KEGGisce:YNL172W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71448 Genomic DNA. Translation: CAA96060.1 .
    BK006947 Genomic DNA. Translation: DAA10378.2 .
    PIRi S63127.
    RefSeqi NP_014227.4. NM_001183010.4.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35658. 50 interactions.
    DIPi DIP-970N.
    IntActi P53886. 13 interactions.
    MINTi MINT-633685.
    STRINGi 4932.YNL172W.

    Proteomic databases

    MaxQBi P53886.
    PaxDbi P53886.
    PeptideAtlasi P53886.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL172W ; YNL172W ; YNL172W .
    GeneIDi 855549.
    KEGGi sce:YNL172W.

    Organism-specific databases

    CYGDi YNL172w.
    SGDi S000005116. APC1.

    Phylogenomic databases

    eggNOGi NOG316940.
    GeneTreei ENSGT00390000016757.
    HOGENOMi HOG000033985.
    KOi K03348.
    OMAi MYREWAS.
    OrthoDBi EOG7NW6JC.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-33185-MONOMER.

    Miscellaneous databases

    NextBioi 979625.

    Gene expression databases

    Genevestigatori P53886.

    Family and domain databases

    InterProi IPR024990. Apc1.
    [Graphical view ]
    PANTHERi PTHR12827. PTHR12827. 1 hit.
    Pfami PF12859. Apc1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 1547.
      Strain: ATCC 204508 / S288c.
    3. "Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae."
      Zachariae W., Shin T.H., Galova M., Obermaier B., Nasmyth K.
      Science 274:1201-1204(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    4. "Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex."
      Hall M.C., Torres M.P., Schroeder G.K., Borchers C.H.
      J. Biol. Chem. 278:16698-16705(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MND2.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAPC1_YEAST
    AccessioniPrimary (citable) accession number: P53886
    Secondary accession number(s): D6W112
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 178 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3