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Protein

Nucleolar protein 13

Gene

NOP13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33187-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 13
Gene namesi
Name:NOP13
Ordered Locus Names:YNL175C
ORF Names:N1665
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL175C.
SGDiS000005119. NOP13.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • preribosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 403402Nucleolar protein 13PRO_0000082036Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei105 – 1051PhosphothreonineCombined sources
Modified residuei335 – 3351PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP53883.
PeptideAtlasiP53883.

PTM databases

iPTMnetiP53883.

Interactioni

Protein-protein interaction databases

BioGridi35656. 83 interactions.
DIPiDIP-4331N.
IntActiP53883. 70 interactions.
MINTiMINT-479067.

Structurei

3D structure databases

ProteinModelPortaliP53883.
SMRiP53883. Positions 119-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 21995RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini239 – 31779RRM 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000193653.
InParanoidiP53883.
OMAiKSCRKIA.
OrthoDBiEOG706125.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETELSKED AVTKKDNEEQ VKKALLDPTK KRKAEDEIEI DLKSSIPLSK
60 70 80 90 100
KQKRLLRRGK VTLEELNAKY NIDPKSIEEY KEDAEKKKSG ASEKDAQGEE
110 120 130 140 150
STINTPTGDE SGEVVKKKKK DENKYGVWIG NLSFDTTKDD LVRFFIAKTK
160 170 180 190 200
DNEDEKSRVT EQDITRLSMP RVAAKNSNAM KNKGFCYMFF KNVEQMKAVL
210 220 230 240 250
ELSESHLNGR NMLIKDSENY SGRPDKDDLV AMSKNPPSRI LFVGNLSFDV
260 270 280 290 300
TDDLLRKHFQ HCGDIVKIRM ATFEDSGKCK GFAFIDFKNE EGSTNALKDK
310 320 330 340 350
SCRKIAGRPL RMEYGEDRSK RQVRKKVENV SRNNSSSFDI SNNKGYDRAG
360 370 380 390 400
QDNGSKPEYK RSNANRRPPV DSNNRTKSSV ALATAQRGSA AIVPSQGKKV

KFD
Length:403
Mass (Da):45,609
Last modified:September 21, 2011 - v2
Checksum:iFB1A0FB46B13C201
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti296 – 2961A → E in CAA96066 (PubMed:9169873).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71451 Genomic DNA. Translation: CAA96066.1.
BK006947 Genomic DNA. Translation: DAA10376.2.
PIRiS63130.
RefSeqiNP_014224.2. NM_001183013.2.

Genome annotation databases

EnsemblFungiiYNL175C; YNL175C; YNL175C.
GeneIDi855547.
KEGGisce:YNL175C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71451 Genomic DNA. Translation: CAA96066.1.
BK006947 Genomic DNA. Translation: DAA10376.2.
PIRiS63130.
RefSeqiNP_014224.2. NM_001183013.2.

3D structure databases

ProteinModelPortaliP53883.
SMRiP53883. Positions 119-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35656. 83 interactions.
DIPiDIP-4331N.
IntActiP53883. 70 interactions.
MINTiMINT-479067.

PTM databases

iPTMnetiP53883.

Proteomic databases

MaxQBiP53883.
PeptideAtlasiP53883.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL175C; YNL175C; YNL175C.
GeneIDi855547.
KEGGisce:YNL175C.

Organism-specific databases

EuPathDBiFungiDB:YNL175C.
SGDiS000005119. NOP13.

Phylogenomic databases

HOGENOMiHOG000193653.
InParanoidiP53883.
OMAiKSCRKIA.
OrthoDBiEOG706125.

Enzyme and pathway databases

BioCyciYEAST:G3O-33187-MONOMER.

Miscellaneous databases

PROiP53883.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 296.
    Strain: ATCC 204508 / S288c.
  3. "Nucleolar protein Nop12p participates in synthesis of 25S rRNA in Saccharomyces cerevisiae."
    Wu K., Wu P., Aris J.P.
    Nucleic Acids Res. 29:2938-2949(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-105 AND SER-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOP13_YEAST
AccessioniPrimary (citable) accession number: P53883
Secondary accession number(s): D6W110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: June 8, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.