P53878 (YNS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized oxidoreductase YNL181W
EC=1.-.-.-

Gene names

Ordered Locus Names:	YNL181W
ORF Names:	N1640

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential Ref.3.
Miscellaneous	Present with 13900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.3PubMed 14690591. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay PubMed 14690591. Source: SGD
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor Inferred from sequence or structural similarity PubMed 9811845. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Uncharacterized oxidoreductase YNL181W		PRO_0000203405
Regions
	Topological domain	1 – 290	290	Lumenal Potential
	Transmembrane	291 – 311	21	Helical; Potential
	Topological domain	312 – 407	96	Cytoplasmic Potential
	Nucleotide binding	57 – 79	23	NAD or NADP By similarity
	Coiled coil	361 – 390	30	Potential
Experimental info
	Sequence conflict	262	1	A → P in CAA96073. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53878 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: E57B677EB79BC2C9	FASTA	407	46,503
	10 20 30 40 50 60 MPLNIIGTAL LDGTDKIPYY QTIKKVAPYV LATGAIKYWS RGPSNTWERK LHGKVYLVTG 70 80 90 100 110 120 ATSQGMGTSV AYKMAELGAQ LIILTREVDE WVTEWCEELR EKTKNELIFV EKCDLSNLWE 130 140 150 160 170 180 IRKFATSWLD NSPPRRLDGV IVMSGDMEPW GIPKISLPQR RSSKDGLELQ IATNYVAIFH 190 200 210 220 230 240 LLNLLQPSFK AQPPDRDVRI ILATCWLQVV GDINIEDPLW QNAKYKSALK FFASSKLQLG 250 260 270 280 290 300 LSMMELQRRL TEDIKNQKTN GAERTGKNVT ITMVQPGTMR SNSLRRVISN GSVVLLIILY 310 320 330 340 350 360 CILLYPILWL FTKSGRRGDQ SFLYALMTPE LEEVNLKDTK VKYISDCSIV KFARKEFDDE 370 380 390 400 ELQKKLFDNT ERDILQLEKK VAAKRNANKT GNQNSKKKSQ NKSRKDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 262. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71457 Genomic DNA. Translation: CAA96073.1. BK006947 Genomic DNA. Translation: DAA10371.2.
PIR	S63136.
RefSeq	NP_014218.2. NM_001183019.2.
3D structure databases
ProteinModelPortal	P53878.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6413N.
IntAct	P53878. 2 interactions.
STRING	4932.YNL181W.
Proteomic databases
PaxDb	P53878.
PeptideAtlas	P53878.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL181W; YNL181W; YNL181W.
GeneID	855540.
KEGG	sce:YNL181W.
Organism-specific databases
CYGD	YNL181w.
SGD	S000005125. YNL181W.
Phylogenomic databases
eggNOG	COG1028.
HOGENOM	HOG000175307.
OMA	TKWLDNQ.
OrthoDB	EOG4Z65XB.
Gene expression databases
Genevestigator	P53878.
GermOnline	YNL181W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979604.

Entry information

Entry name

YNS1_YEAST

Accession

Primary (citable) accession number: P53878
Secondary accession number(s): D6W105

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program