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P53877 (IPI3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-rRNA-processing protein IPI3
Alternative name(s):
Involved in processing IST2 protein 3
Gene names
Name:IPI3
Ordered Locus Names:YNL182C
ORF Names:N1636
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA. Ref.4 Ref.5

Subunit structure

Component of the RIX1 complex, composed of at least IPI1, IPI3 and RIX1. The complex interacts with MDN1 and the pre-60S ribosome particles. Ref.3 Ref.4 Ref.5 Ref.6

Subcellular location

Nucleus Ref.4.

Sequence similarities

Belongs to the WD repeat IPI3 family.

Contains 4 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RIX1P388837EBI-29063,EBI-24899

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Pre-rRNA-processing protein IPI3
PRO_0000051484

Regions

Repeat90 – 13344WD 1
Repeat137 – 17640WD 2
Repeat187 – 23448WD 3
Repeat342 – 38342WD 4

Amino acid modifications

Modified residue3881Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P53877 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 8EED6854DF9405A6

FASTA55561,773
        10         20         30         40         50         60 
MDEQVIFTTN TSGTIASVHS FEQINLRQCS TQSRNSCVQV GNKYLFIAQA QKALINVYNL 

        70         80         90        100        110        120 
SGSFKRESVE QRLPLPEILK CLEVVENDGV QYDRIQGVNH NLPDFNLPYL LLGSTESGKL 

       130        140        150        160        170        180 
YIWELNSGIL LNVKPMAHYQ SITKIKSILN GKYIITSGND SRVIIWQTVD LVSASNDDPK 

       190        200        210        220        230        240 
PLCILHDHTL PVTDFQVSSS QGKFLSCTDT KLFTVSQDAT IRCYDLSLIG SKKKQKANEN 

       250        260        270        280        290        300 
DVSIGKTPVL LATFTTPYSI KSIVLDPADR ACYIGTAEGC FSLNLFYKLK GNAIVNLLQS 

       310        320        330        340        350        360 
AGVNTVQKGR VFSLVQRNSL TGGENEDLDA LYAMGQLVCE NVLNSNVSCL EISMDGTLLL 

       370        380        390        400        410        420 
IGDTEGKVSI AEIYSKQIIR TIQTLTTSQD SVGEVTNLLT NPYRLERGNL LFEGESKGKQ 

       430        440        450        460        470        480 
PSNNNGHNFM KIPNLQRVIF DGKNKGHLHD IWYQIGEPEA ETDPNLALPL NDFNAYLEQV 

       490        500        510        520        530        540 
KTQESIFSHI GKVSSNVKVI DNKIDATSSL DSNAAKDEEI TELKTNIEAL THAYKELRDM 

       550 
HEKLYEEHQQ MLDKQ 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Assigning function to yeast proteins by integration of technologies."
Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M., Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E., Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.
Mol. Cell 12:1353-1365(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits."
Galani K., Nissan T.A., Petfalski E., Tollervey D., Hurt E.
J. Biol. Chem. 279:55411-55418(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE RIX1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"High-definition macromolecular composition of yeast RNA-processing complexes."
Krogan N.J., Peng W.-T., Cagney G., Robinson M.D., Haw R., Zhong G., Guo X., Zhang X., Canadien V., Richards D.P., Beattie B.K., Lalev A., Zhang W., Davierwala A.P., Mnaimneh S., Starostine A., Tikuisis A.P., Grigull J. expand/collapse author list , Datta N., Bray J.E., Hughes T.R., Emili A., Greenblatt J.F.
Mol. Cell 13:225-239(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, FUNCTION OF THE RIX1 COMPLEX.
[6]"A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits."
Nissan T.A., Galani K., Maco B., Tollervey D., Aebi U., Hurt E.
Mol. Cell 15:295-301(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, INTERACTION WITH MDN1 AND PRE-60S RIBOSOMAL PARTICLES, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-388, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71458 Genomic DNA. Translation: CAA96075.1.
BK006947 Genomic DNA. Translation: DAA10370.1.
PIRS63137.
RefSeqNP_014217.1. NM_001183020.1.

3D structure databases

ProteinModelPortalP53877.
SMRP53877. Positions 303-376.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35650. 56 interactions.
DIPDIP-4297N.
IntActP53877. 20 interactions.
MINTMINT-529654.
STRING4932.YNL182C.

Proteomic databases

MaxQBP53877.
PaxDbP53877.
PeptideAtlasP53877.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL182C; YNL182C; YNL182C.
GeneID855539.
KEGGsce:YNL182C.

Organism-specific databases

CYGDYNL182c.
SGDS000005126. IPI3.

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000113091.
KOK14829.
OMADEQVIFT.
OrthoDBEOG7SFJ5S.

Enzyme and pathway databases

BioCycYEAST:G3O-33193-MONOMER.

Gene expression databases

GenevestigatorP53877.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
PROSITEPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979601.

Entry information

Entry nameIPI3_YEAST
AccessionPrimary (citable) accession number: P53877
Secondary accession number(s): D6W104
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families