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P53877

- IPI3_YEAST

UniProt

P53877 - IPI3_YEAST

Protein

Pre-rRNA-processing protein IPI3

Gene

IPI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.2 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
    2. regulation of DNA-dependent DNA replication initiation Source: SGD
    3. ribosomal large subunit assembly Source: SGD
    4. rRNA processing Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33193-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-rRNA-processing protein IPI3
    Alternative name(s):
    Involved in processing IST2 protein 3
    Gene namesi
    Name:IPI3
    Ordered Locus Names:YNL182C
    ORF Names:N1636
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL182c.
    SGDiS000005126. IPI3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nuclear pre-replicative complex Source: SGD
    2. nucleoplasm Source: SGD
    3. Rix1 complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 555555Pre-rRNA-processing protein IPI3PRO_0000051484Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei388 – 3881Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53877.
    PaxDbiP53877.
    PeptideAtlasiP53877.

    Expressioni

    Gene expression databases

    GenevestigatoriP53877.

    Interactioni

    Subunit structurei

    Component of the RIX1 complex, composed of at least IPI1, IPI3 and RIX1. The complex interacts with MDN1 and the pre-60S ribosome particles.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RIX1P388837EBI-29063,EBI-24899

    Protein-protein interaction databases

    BioGridi35650. 56 interactions.
    DIPiDIP-4297N.
    IntActiP53877. 20 interactions.
    MINTiMINT-529654.
    STRINGi4932.YNL182C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53877.
    SMRiP53877. Positions 303-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati90 – 13344WD 1Add
    BLAST
    Repeati137 – 17640WD 2Add
    BLAST
    Repeati187 – 23448WD 3Add
    BLAST
    Repeati342 – 38342WD 4Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat IPI3 family.Curated
    Contains 4 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000113091.
    KOiK14829.
    OMAiDEQVIFT.
    OrthoDBiEOG7SFJ5S.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 3 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53877-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEQVIFTTN TSGTIASVHS FEQINLRQCS TQSRNSCVQV GNKYLFIAQA    50
    QKALINVYNL SGSFKRESVE QRLPLPEILK CLEVVENDGV QYDRIQGVNH 100
    NLPDFNLPYL LLGSTESGKL YIWELNSGIL LNVKPMAHYQ SITKIKSILN 150
    GKYIITSGND SRVIIWQTVD LVSASNDDPK PLCILHDHTL PVTDFQVSSS 200
    QGKFLSCTDT KLFTVSQDAT IRCYDLSLIG SKKKQKANEN DVSIGKTPVL 250
    LATFTTPYSI KSIVLDPADR ACYIGTAEGC FSLNLFYKLK GNAIVNLLQS 300
    AGVNTVQKGR VFSLVQRNSL TGGENEDLDA LYAMGQLVCE NVLNSNVSCL 350
    EISMDGTLLL IGDTEGKVSI AEIYSKQIIR TIQTLTTSQD SVGEVTNLLT 400
    NPYRLERGNL LFEGESKGKQ PSNNNGHNFM KIPNLQRVIF DGKNKGHLHD 450
    IWYQIGEPEA ETDPNLALPL NDFNAYLEQV KTQESIFSHI GKVSSNVKVI 500
    DNKIDATSSL DSNAAKDEEI TELKTNIEAL THAYKELRDM HEKLYEEHQQ 550
    MLDKQ 555
    Length:555
    Mass (Da):61,773
    Last modified:October 1, 1996 - v1
    Checksum:i8EED6854DF9405A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71458 Genomic DNA. Translation: CAA96075.1.
    BK006947 Genomic DNA. Translation: DAA10370.1.
    PIRiS63137.
    RefSeqiNP_014217.1. NM_001183020.1.

    Genome annotation databases

    EnsemblFungiiYNL182C; YNL182C; YNL182C.
    GeneIDi855539.
    KEGGisce:YNL182C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71458 Genomic DNA. Translation: CAA96075.1 .
    BK006947 Genomic DNA. Translation: DAA10370.1 .
    PIRi S63137.
    RefSeqi NP_014217.1. NM_001183020.1.

    3D structure databases

    ProteinModelPortali P53877.
    SMRi P53877. Positions 303-376.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35650. 56 interactions.
    DIPi DIP-4297N.
    IntActi P53877. 20 interactions.
    MINTi MINT-529654.
    STRINGi 4932.YNL182C.

    Proteomic databases

    MaxQBi P53877.
    PaxDbi P53877.
    PeptideAtlasi P53877.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL182C ; YNL182C ; YNL182C .
    GeneIDi 855539.
    KEGGi sce:YNL182C.

    Organism-specific databases

    CYGDi YNL182c.
    SGDi S000005126. IPI3.

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000113091.
    KOi K14829.
    OMAi DEQVIFT.
    OrthoDBi EOG7SFJ5S.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33193-MONOMER.

    Miscellaneous databases

    NextBioi 979601.

    Gene expression databases

    Genevestigatori P53877.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 3 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits."
      Galani K., Nissan T.A., Petfalski E., Tollervey D., Hurt E.
      J. Biol. Chem. 279:55411-55418(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE RIX1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, FUNCTION OF THE RIX1 COMPLEX.
    6. "A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits."
      Nissan T.A., Galani K., Maco B., Tollervey D., Aebi U., Hurt E.
      Mol. Cell 15:295-301(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RIX1 COMPLEX, INTERACTION WITH MDN1 AND PRE-60S RIBOSOMAL PARTICLES, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-388, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIPI3_YEAST
    AccessioniPrimary (citable) accession number: P53877
    Secondary accession number(s): D6W104
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3