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Protein

Ubiquitin carboxyl-terminal hydrolase 10

Gene

UBP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei371 – 3711NucleophilePROSITE-ProRule annotation
Active sitei691 – 6911Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  1. chromatin silencing at telomere Source: SGD
  2. histone deubiquitination Source: SGD
  3. protein deubiquitination Source: SGD
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33197-MONOMER.

Protein family/group databases

MEROPSiC19.088.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 10 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 10
Disrupter of telomere silencing protein 4
Ubiquitin thioesterase 10
Ubiquitin-specific-processing protease 10
Gene namesi
Name:UBP10
Synonyms:DOT4
Ordered Locus Names:YNL186W
ORF Names:N1619
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL186w.
SGDiS000005130. UBP10.

Subcellular locationi

Chromosometelomere Curated

GO - Cellular componenti

  1. chromosome, telomeric region Source: UniProtKB-SubCell
  2. nucleolus Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 792792Ubiquitin carboxyl-terminal hydrolase 10PRO_0000080595Add
BLAST

Proteomic databases

MaxQBiP53874.
PaxDbiP53874.
PeptideAtlasiP53874.

Expressioni

Gene expression databases

GenevestigatoriP53874.

Interactioni

Protein-protein interaction databases

BioGridi35647. 166 interactions.
DIPiDIP-6664N.
IntActiP53874. 21 interactions.
MINTiMINT-643556.
STRINGi4932.YNL186W.

Structurei

3D structure databases

ProteinModelPortaliP53874.
SMRiP53874. Positions 363-733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini362 – 733372USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi127 – 1304Poly-Ser
Compositional biasi138 – 1447Poly-Glu
Compositional biasi252 – 2554Poly-Glu
Compositional biasi302 – 3098Poly-Glu
Compositional biasi556 – 5627Poly-Ser

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121904.
HOGENOMiHOG000248158.
InParanoidiP53874.
KOiK11873.
OMAiYVCEKCH.
OrthoDBiEOG7R2BSX.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQESIKPL VDRILSNPLQ FNAAMISNKS NNNDTSAAPE NSSYIVIGKQ
60 70 80 90 100
HNNNSNSTAI AATAESKQIK ENNLIDRPNG KKTNTVPKSM AEALLLYTSK
110 120 130 140 150
NDKDAADATG AKKSAELSTE LSTEPPSSSS EDDKVGKEEE EEGEIFHEAR
160 170 180 190 200
DYVEPRKASL KERDNADKGD GEDIGEDIGE DIGEDIGEDI GEDIGENLGS
210 220 230 240 250
PLATIDDSSN ENEKEKRKEL STSISSDDEI EDDEDEDDMD YDSSAMEKEL
260 270 280 290 300
PEEEENDSSS KISEGEKKSL YQDLMENSTV EVNRYEPVNN TKENGNRNPK
310 320 330 340 350
GEEEEEEEEE LKHKSRSITP PVTISNLSNF YQFNENINDR GSLNSTRIVK
360 370 380 390 400
NWGDKFTNLK PRGLLNHGVT CYTNAAVQAM LHIPSIQHYL FDILMGKYDS
410 420 430 440 450
TISKNSVSYT LAETSKKMWL PVSKNPRKNV SASYINPKHL ISRLDDINCM
460 470 480 490 500
MSEWQQEDSH EYFMSLMSRL QEDSVPKGHK LIESIIYDIF GGLLKQIVTC
510 520 530 540 550
KSCGSISKTE QPFYDLSLHL KGKKKLDPNS DLSSDSINGT SATTSTTTSN
560 570 580 590 600
AATKPSLSSS SSVNLNNGSP FAAASDLSSA NRRFSIEKSI KDFFNPELIK
610 620 630 640 650
VDKEQKGYVC EKCHKTTNAV KHSSILRAPE TLLVHLKKFR FNGTSSSKMK
660 670 680 690 700
QAVSYPMFLD LTEYCESKEL PVKYQLLSVV VHEGRSLSSG HYIAHCKQPD
710 720 730 740 750
GSWATYDDEY INIISERDVL KEPNAYYLLY TRLTPKSVPL PLAKSAMATG
760 770 780 790
NVTSKSKQEQ AVNEPNNRPL KINSKKNNRK KWKKNKKRKF TK
Length:792
Mass (Da):88,531
Last modified:September 21, 2011 - v2
Checksum:iAA2DE6DB0C560E37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti310 – 3101E → D in CAA96080 (PubMed:9169873).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71462 Genomic DNA. Translation: CAA96080.1.
BK006947 Genomic DNA. Translation: DAA10367.2.
PIRiS63141.
RefSeqiNP_014213.2. NM_001183024.2.

Genome annotation databases

EnsemblFungiiYNL186W; YNL186W; YNL186W.
GeneIDi855535.
KEGGisce:YNL186W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71462 Genomic DNA. Translation: CAA96080.1.
BK006947 Genomic DNA. Translation: DAA10367.2.
PIRiS63141.
RefSeqiNP_014213.2. NM_001183024.2.

3D structure databases

ProteinModelPortaliP53874.
SMRiP53874. Positions 363-733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35647. 166 interactions.
DIPiDIP-6664N.
IntActiP53874. 21 interactions.
MINTiMINT-643556.
STRINGi4932.YNL186W.

Protein family/group databases

MEROPSiC19.088.

Proteomic databases

MaxQBiP53874.
PaxDbiP53874.
PeptideAtlasiP53874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL186W; YNL186W; YNL186W.
GeneIDi855535.
KEGGisce:YNL186W.

Organism-specific databases

CYGDiYNL186w.
SGDiS000005130. UBP10.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121904.
HOGENOMiHOG000248158.
InParanoidiP53874.
KOiK11873.
OMAiYVCEKCH.
OrthoDBiEOG7R2BSX.

Enzyme and pathway databases

BioCyciYEAST:G3O-33197-MONOMER.

Miscellaneous databases

NextBioi979592.

Gene expression databases

GenevestigatoriP53874.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 310.
    Strain: ATCC 204508 / S288c.
  3. "Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae."
    Singer M.S., Kahana A., Wolf A.J., Meisinger L.L., Peterson S.E., Goggin C., Mahowald M., Gottschling D.E.
    Genetics 150:613-632(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP10_YEAST
AccessioniPrimary (citable) accession number: P53874
Secondary accession number(s): D6W101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: February 4, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.