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P53874 (UBP10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 10

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 10
Disrupter of telomere silencing protein 4
Ubiquitin thioesterase 10
Ubiquitin-specific-processing protease 10
Gene names
Name:UBP10
Synonyms:DOT4
Ordered Locus Names:YNL186W
ORF Names:N1619
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length792 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes. Ref.3

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Chromosometelomere Potential.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 792792Ubiquitin carboxyl-terminal hydrolase 10
PRO_0000080595

Regions

Domain362 – 733372USP
Compositional bias127 – 1304Poly-Ser
Compositional bias138 – 1447Poly-Glu
Compositional bias252 – 2554Poly-Glu
Compositional bias302 – 3098Poly-Glu
Compositional bias556 – 5627Poly-Ser

Sites

Active site3711Nucleophile By similarity
Active site6911Proton acceptor By similarity

Experimental info

Sequence conflict3101E → D in CAA96080. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53874 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: AA2DE6DB0C560E37

FASTA79288,531
        10         20         30         40         50         60 
MTTQESIKPL VDRILSNPLQ FNAAMISNKS NNNDTSAAPE NSSYIVIGKQ HNNNSNSTAI 

        70         80         90        100        110        120 
AATAESKQIK ENNLIDRPNG KKTNTVPKSM AEALLLYTSK NDKDAADATG AKKSAELSTE 

       130        140        150        160        170        180 
LSTEPPSSSS EDDKVGKEEE EEGEIFHEAR DYVEPRKASL KERDNADKGD GEDIGEDIGE 

       190        200        210        220        230        240 
DIGEDIGEDI GEDIGENLGS PLATIDDSSN ENEKEKRKEL STSISSDDEI EDDEDEDDMD 

       250        260        270        280        290        300 
YDSSAMEKEL PEEEENDSSS KISEGEKKSL YQDLMENSTV EVNRYEPVNN TKENGNRNPK 

       310        320        330        340        350        360 
GEEEEEEEEE LKHKSRSITP PVTISNLSNF YQFNENINDR GSLNSTRIVK NWGDKFTNLK 

       370        380        390        400        410        420 
PRGLLNHGVT CYTNAAVQAM LHIPSIQHYL FDILMGKYDS TISKNSVSYT LAETSKKMWL 

       430        440        450        460        470        480 
PVSKNPRKNV SASYINPKHL ISRLDDINCM MSEWQQEDSH EYFMSLMSRL QEDSVPKGHK 

       490        500        510        520        530        540 
LIESIIYDIF GGLLKQIVTC KSCGSISKTE QPFYDLSLHL KGKKKLDPNS DLSSDSINGT 

       550        560        570        580        590        600 
SATTSTTTSN AATKPSLSSS SSVNLNNGSP FAAASDLSSA NRRFSIEKSI KDFFNPELIK 

       610        620        630        640        650        660 
VDKEQKGYVC EKCHKTTNAV KHSSILRAPE TLLVHLKKFR FNGTSSSKMK QAVSYPMFLD 

       670        680        690        700        710        720 
LTEYCESKEL PVKYQLLSVV VHEGRSLSSG HYIAHCKQPD GSWATYDDEY INIISERDVL 

       730        740        750        760        770        780 
KEPNAYYLLY TRLTPKSVPL PLAKSAMATG NVTSKSKQEQ AVNEPNNRPL KINSKKNNRK 

       790 
KWKKNKKRKF TK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 310.
Strain: ATCC 204508 / S288c.
[3]"Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae."
Singer M.S., Kahana A., Wolf A.J., Meisinger L.L., Peterson S.E., Goggin C., Mahowald M., Gottschling D.E.
Genetics 150:613-632(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71462 Genomic DNA. Translation: CAA96080.1.
BK006947 Genomic DNA. Translation: DAA10367.2.
PIRS63141.
RefSeqNP_014213.2. NM_001183024.2.

3D structure databases

ProteinModelPortalP53874.
SMRP53874. Positions 363-733.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35647. 155 interactions.
DIPDIP-6664N.
IntActP53874. 21 interactions.
MINTMINT-643556.
STRING4932.YNL186W.

Protein family/group databases

MEROPSC19.088.

Proteomic databases

MaxQBP53874.
PaxDbP53874.
PeptideAtlasP53874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL186W; YNL186W; YNL186W.
GeneID855535.
KEGGsce:YNL186W.

Organism-specific databases

CYGDYNL186w.
SGDS000005130. UBP10.

Phylogenomic databases

eggNOGCOG5533.
GeneTreeENSGT00750000117701.
HOGENOMHOG000248158.
KOK11873.
OMAYVCEKCH.
OrthoDBEOG7R2BSX.

Enzyme and pathway databases

BioCycYEAST:G3O-33197-MONOMER.

Gene expression databases

GenevestigatorP53874.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979592.

Entry information

Entry nameUBP10_YEAST
AccessionPrimary (citable) accession number: P53874
Secondary accession number(s): D6W101
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries