P53874 (UBP10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 10
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 10
Disrupter of telomere silencing protein 4
Ubiquitin thioesterase 10
Ubiquitin-specific-processing protease 10

Gene names

Name:	UBP10
Synonyms:	DOT4
Ordered Locus Names:	YNL186W
ORF Names:	N1619

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes. Ref.3
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Chromosome › telomere Potential.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Chromosome Telomere
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin silencing at telomere Inferred from mutant phenotype PubMed 15721261. Source: SGD protein deubiquitination Inferred from mutant phenotype PubMed 15721261. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 10490600PubMed 11914276. Source: SGD
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay PubMed 10490600. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 792	792	Ubiquitin carboxyl-terminal hydrolase 10		PRO_0000080595
Regions
	Compositional bias	127 – 130	4	Poly-Ser
	Compositional bias	138 – 144	7	Poly-Glu
	Compositional bias	252 – 255	4	Poly-Glu
	Compositional bias	302 – 309	8	Poly-Glu
	Compositional bias	556 – 562	7	Poly-Ser
Sites
	Active site	371	1	Nucleophile By similarity
	Active site	691	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	310	1	E → D in CAA96080. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53874 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: AA2DE6DB0C560E37	FASTA	792	88,531
	10 20 30 40 50 60 MTTQESIKPL VDRILSNPLQ FNAAMISNKS NNNDTSAAPE NSSYIVIGKQ HNNNSNSTAI 70 80 90 100 110 120 AATAESKQIK ENNLIDRPNG KKTNTVPKSM AEALLLYTSK NDKDAADATG AKKSAELSTE 130 140 150 160 170 180 LSTEPPSSSS EDDKVGKEEE EEGEIFHEAR DYVEPRKASL KERDNADKGD GEDIGEDIGE 190 200 210 220 230 240 DIGEDIGEDI GEDIGENLGS PLATIDDSSN ENEKEKRKEL STSISSDDEI EDDEDEDDMD 250 260 270 280 290 300 YDSSAMEKEL PEEEENDSSS KISEGEKKSL YQDLMENSTV EVNRYEPVNN TKENGNRNPK 310 320 330 340 350 360 GEEEEEEEEE LKHKSRSITP PVTISNLSNF YQFNENINDR GSLNSTRIVK NWGDKFTNLK 370 380 390 400 410 420 PRGLLNHGVT CYTNAAVQAM LHIPSIQHYL FDILMGKYDS TISKNSVSYT LAETSKKMWL 430 440 450 460 470 480 PVSKNPRKNV SASYINPKHL ISRLDDINCM MSEWQQEDSH EYFMSLMSRL QEDSVPKGHK 490 500 510 520 530 540 LIESIIYDIF GGLLKQIVTC KSCGSISKTE QPFYDLSLHL KGKKKLDPNS DLSSDSINGT 550 560 570 580 590 600 SATTSTTTSN AATKPSLSSS SSVNLNNGSP FAAASDLSSA NRRFSIEKSI KDFFNPELIK 610 620 630 640 650 660 VDKEQKGYVC EKCHKTTNAV KHSSILRAPE TLLVHLKKFR FNGTSSSKMK QAVSYPMFLD 670 680 690 700 710 720 LTEYCESKEL PVKYQLLSVV VHEGRSLSSG HYIAHCKQPD GSWATYDDEY INIISERDVL 730 740 750 760 770 780 KEPNAYYLLY TRLTPKSVPL PLAKSAMATG NVTSKSKQEQ AVNEPNNRPL KINSKKNNRK 790 KWKKNKKRKF TK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 310. Strain: ATCC 204508 / S288c.
[3]	"Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae." Singer M.S., Kahana A., Wolf A.J., Meisinger L.L., Peterson S.E., Goggin C., Mahowald M., Gottschling D.E. Genetics 150:613-632(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71462 Genomic DNA. Translation: CAA96080.1. BK006947 Genomic DNA. Translation: DAA10367.2.
PIR	S63141.
RefSeq	NP_014213.2. NM_001183024.2.
3D structure databases
ProteinModelPortal	P53874.
SMR	P53874. Positions 363-733.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6664N.
IntAct	P53874. 21 interactions.
MINT	MINT-643556.
STRING	4932.YNL186W.
Protein family/group databases
MEROPS	C19.088.
Proteomic databases
PaxDb	P53874.
PeptideAtlas	P53874.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL186W; YNL186W; YNL186W.
GeneID	855535.
KEGG	sce:YNL186W.
Organism-specific databases
CYGD	YNL186w.
SGD	S000005130. UBP10.
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00600000085270.
HOGENOM	HOG000248158.
KO	K11873.
OMA	YVCEKCH.
OrthoDB	EOG4JHGQN.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33197-MONOMER.
Gene expression databases
Genevestigator	P53874.
GermOnline	YNL186W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. False negative. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979592.

Entry information

Entry name

UBP10_YEAST

Accession

Primary (citable) accession number: P53874
Secondary accession number(s): D6W101

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 21, 2011
Last modified:	May 29, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program