Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53874

- UBP10_YEAST

UniProt

P53874 - UBP10_YEAST

Protein

Ubiquitin carboxyl-terminal hydrolase 10

Gene

UBP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Has a role in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei371 – 3711NucleophilePROSITE-ProRule annotation
    Active sitei691 – 6911Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: SGD

    GO - Biological processi

    1. chromatin silencing at telomere Source: SGD
    2. histone deubiquitination Source: SGD
    3. protein deubiquitination Source: SGD
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33197-MONOMER.

    Protein family/group databases

    MEROPSiC19.088.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 10 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 10
    Disrupter of telomere silencing protein 4
    Ubiquitin thioesterase 10
    Ubiquitin-specific-processing protease 10
    Gene namesi
    Name:UBP10
    Synonyms:DOT4
    Ordered Locus Names:YNL186W
    ORF Names:N1619
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL186w.
    SGDiS000005130. UBP10.

    Subcellular locationi

    Chromosometelomere Curated

    GO - Cellular componenti

    1. chromosome, telomeric region Source: UniProtKB-SubCell
    2. nucleolus Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Chromosome, Telomere

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 792792Ubiquitin carboxyl-terminal hydrolase 10PRO_0000080595Add
    BLAST

    Proteomic databases

    MaxQBiP53874.
    PaxDbiP53874.
    PeptideAtlasiP53874.

    Expressioni

    Gene expression databases

    GenevestigatoriP53874.

    Interactioni

    Protein-protein interaction databases

    BioGridi35647. 155 interactions.
    DIPiDIP-6664N.
    IntActiP53874. 21 interactions.
    MINTiMINT-643556.
    STRINGi4932.YNL186W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53874.
    SMRiP53874. Positions 363-733.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini362 – 733372USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi127 – 1304Poly-Ser
    Compositional biasi138 – 1447Poly-Glu
    Compositional biasi252 – 2554Poly-Glu
    Compositional biasi302 – 3098Poly-Glu
    Compositional biasi556 – 5627Poly-Ser

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    GeneTreeiENSGT00750000117701.
    HOGENOMiHOG000248158.
    KOiK11873.
    OMAiYVCEKCH.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53874-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTQESIKPL VDRILSNPLQ FNAAMISNKS NNNDTSAAPE NSSYIVIGKQ    50
    HNNNSNSTAI AATAESKQIK ENNLIDRPNG KKTNTVPKSM AEALLLYTSK 100
    NDKDAADATG AKKSAELSTE LSTEPPSSSS EDDKVGKEEE EEGEIFHEAR 150
    DYVEPRKASL KERDNADKGD GEDIGEDIGE DIGEDIGEDI GEDIGENLGS 200
    PLATIDDSSN ENEKEKRKEL STSISSDDEI EDDEDEDDMD YDSSAMEKEL 250
    PEEEENDSSS KISEGEKKSL YQDLMENSTV EVNRYEPVNN TKENGNRNPK 300
    GEEEEEEEEE LKHKSRSITP PVTISNLSNF YQFNENINDR GSLNSTRIVK 350
    NWGDKFTNLK PRGLLNHGVT CYTNAAVQAM LHIPSIQHYL FDILMGKYDS 400
    TISKNSVSYT LAETSKKMWL PVSKNPRKNV SASYINPKHL ISRLDDINCM 450
    MSEWQQEDSH EYFMSLMSRL QEDSVPKGHK LIESIIYDIF GGLLKQIVTC 500
    KSCGSISKTE QPFYDLSLHL KGKKKLDPNS DLSSDSINGT SATTSTTTSN 550
    AATKPSLSSS SSVNLNNGSP FAAASDLSSA NRRFSIEKSI KDFFNPELIK 600
    VDKEQKGYVC EKCHKTTNAV KHSSILRAPE TLLVHLKKFR FNGTSSSKMK 650
    QAVSYPMFLD LTEYCESKEL PVKYQLLSVV VHEGRSLSSG HYIAHCKQPD 700
    GSWATYDDEY INIISERDVL KEPNAYYLLY TRLTPKSVPL PLAKSAMATG 750
    NVTSKSKQEQ AVNEPNNRPL KINSKKNNRK KWKKNKKRKF TK 792
    Length:792
    Mass (Da):88,531
    Last modified:September 21, 2011 - v2
    Checksum:iAA2DE6DB0C560E37
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti310 – 3101E → D in CAA96080. (PubMed:9169873)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71462 Genomic DNA. Translation: CAA96080.1.
    BK006947 Genomic DNA. Translation: DAA10367.2.
    PIRiS63141.
    RefSeqiNP_014213.2. NM_001183024.2.

    Genome annotation databases

    EnsemblFungiiYNL186W; YNL186W; YNL186W.
    GeneIDi855535.
    KEGGisce:YNL186W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71462 Genomic DNA. Translation: CAA96080.1 .
    BK006947 Genomic DNA. Translation: DAA10367.2 .
    PIRi S63141.
    RefSeqi NP_014213.2. NM_001183024.2.

    3D structure databases

    ProteinModelPortali P53874.
    SMRi P53874. Positions 363-733.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35647. 155 interactions.
    DIPi DIP-6664N.
    IntActi P53874. 21 interactions.
    MINTi MINT-643556.
    STRINGi 4932.YNL186W.

    Protein family/group databases

    MEROPSi C19.088.

    Proteomic databases

    MaxQBi P53874.
    PaxDbi P53874.
    PeptideAtlasi P53874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL186W ; YNL186W ; YNL186W .
    GeneIDi 855535.
    KEGGi sce:YNL186W.

    Organism-specific databases

    CYGDi YNL186w.
    SGDi S000005130. UBP10.

    Phylogenomic databases

    eggNOGi COG5533.
    GeneTreei ENSGT00750000117701.
    HOGENOMi HOG000248158.
    KOi K11873.
    OMAi YVCEKCH.
    OrthoDBi EOG7R2BSX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33197-MONOMER.

    Miscellaneous databases

    NextBioi 979592.

    Gene expression databases

    Genevestigatori P53874.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 310.
      Strain: ATCC 204508 / S288c.
    3. "Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae."
      Singer M.S., Kahana A., Wolf A.J., Meisinger L.L., Peterson S.E., Goggin C., Mahowald M., Gottschling D.E.
      Genetics 150:613-632(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBP10_YEAST
    AccessioniPrimary (citable) accession number: P53874
    Secondary accession number(s): D6W101
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3