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P53871 (DUG3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamine amidotransferase DUG3
Alternative name(s):
Deficient in utilization of glutathione protein 3
GSH degradosomal complex subunit DUG3
Gene names
Name:DUG3
Ordered Locus Names:YNL191W
ORF Names:N1410
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond. Ref.6

Subunit structure

Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3. Ref.6

Subcellular location

Cytoplasm Ref.4 Ref.6.

Miscellaneous

Present with 7300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DUG3 family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 357356Probable glutamine amidotransferase DUG3
PRO_0000203401

Regions

Domain2 – 260259Glutamine amidotransferase type-2

Sites

Active site21For GATase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P53871 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: AFAAEFE7152CFE1A

FASTA35740,181
        10         20         30         40         50         60 
MCRFLIFKGK QPIRLSHLLT RPAHSIINQS FDSRLRLDRR RPMNGDGFGV AYYPLDTELS 

        70         80         90        100        110        120 
EDGPCLFKAI TPAWNNQNLS TLAEKTKSDL VFAHVRASTY GVLSETNCHP FTYHSLCFMH 

       130        140        150        160        170        180 
NGGISNFKGI KRKLLNHIKD EYLNFIQGST DSECAFALFL DTLDKLGYDP KKQDGDFGNV 

       190        200        210        220        230        240 
ALRKAMLRTI DYIRDWTKEA NKDEAHVEPS LLNFAVTDGS TVVVSRYITS KTDEAASLHF 

       250        260        270        280        290        300 
SCGSSFVETS PGEYRVERLD RNQDVIMVAS EPLTFERGDW TAVPTNSILT IKKQTILLHP 

       310        320        330        340        350 
IIDEYYQEDP LYLRSSTLAE SKGLMGSIPL AKAVEKNVPP LEREGRTRPP TAVAHIA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae."
Ganguli D., Kumar C., Bachhawat A.K.
Genetics 175:1137-1151(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE GSH DEGRADOSOMAL COMPLEX, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71467 Genomic DNA. Translation: CAA96085.1.
AY692717 Genomic DNA. Translation: AAT92736.1.
BK006947 Genomic DNA. Translation: DAA10362.1.
PIRS63146.
RefSeqNP_014208.1. NM_001183029.1.

3D structure databases

ProteinModelPortalP53871.
SMRP53871. Positions 2-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35642. 5 interactions.
DIPDIP-3956N.
IntActP53871. 4 interactions.
MINTMINT-501795.
STRING4932.YNL191W.

Proteomic databases

PaxDbP53871.
PeptideAtlasP53871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL191W; YNL191W; YNL191W.
GeneID855530.
KEGGsce:YNL191W.

Organism-specific databases

CYGDYNL191w.
SGDS000005135. DUG3.

Phylogenomic databases

eggNOGCOG0121.
HOGENOMHOG000256686.
KOK07008.
OMAPHNPRIA.
OrthoDBEOG7QNVX7.

Enzyme and pathway databases

BioCycYEAST:G3O-33202-MONOMER.

Gene expression databases

GenevestigatorP53871.

Family and domain databases

InterProIPR017932. GATase_2_dom.
IPR026869. Put_GATase_2.
[Graphical view]
PfamPF13230. GATase_4. 1 hit.
[Graphical view]
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979577.

Entry information

Entry nameDUG3_YEAST
AccessionPrimary (citable) accession number: P53871
Secondary accession number(s): D6W0Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families