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Protein

Probable glutamine amidotransferase DUG3

Gene

DUG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity

GO - Molecular functioni

  • amidophosphoribosyltransferase activity Source: SGD
  • omega peptidase activity Source: SGD
  • peptidase activity Source: SGD

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • glutathione catabolic process Source: SGD
  • proteolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamine amidotransferase DUG3
Alternative name(s):
Deficient in utilization of glutathione protein 3
GSH degradosomal complex subunit DUG3
Gene namesi
Name:DUG3
Ordered Locus Names:YNL191W
ORF Names:N1410
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIV

Organism-specific databases

CYGDiYNL191w.
EuPathDBiFungiDB:YNL191W.
SGDiS000005135. DUG3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • glutathione hydrolase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 357356Probable glutamine amidotransferase DUG3PRO_0000203401Add
BLAST

Proteomic databases

MaxQBiP53871.
PaxDbiP53871.
PeptideAtlasiP53871.

Interactioni

Subunit structurei

Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3.1 Publication

Protein-protein interaction databases

BioGridi35642. 6 interactions.
DIPiDIP-3956N.
IntActiP53871. 4 interactions.
MINTiMINT-501795.

Structurei

3D structure databases

ProteinModelPortaliP53871.
SMRiP53871. Positions 46-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 260259Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DUG3 family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0121.
HOGENOMiHOG000256686.
InParanoidiP53871.
KOiK18802.
OMAiYPHNPRI.
OrthoDBiEOG7QNVX7.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR026869. Put_GATase_2.
[Graphical view]
PfamiPF13230. GATase_4. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRFLIFKGK QPIRLSHLLT RPAHSIINQS FDSRLRLDRR RPMNGDGFGV
60 70 80 90 100
AYYPLDTELS EDGPCLFKAI TPAWNNQNLS TLAEKTKSDL VFAHVRASTY
110 120 130 140 150
GVLSETNCHP FTYHSLCFMH NGGISNFKGI KRKLLNHIKD EYLNFIQGST
160 170 180 190 200
DSECAFALFL DTLDKLGYDP KKQDGDFGNV ALRKAMLRTI DYIRDWTKEA
210 220 230 240 250
NKDEAHVEPS LLNFAVTDGS TVVVSRYITS KTDEAASLHF SCGSSFVETS
260 270 280 290 300
PGEYRVERLD RNQDVIMVAS EPLTFERGDW TAVPTNSILT IKKQTILLHP
310 320 330 340 350
IIDEYYQEDP LYLRSSTLAE SKGLMGSIPL AKAVEKNVPP LEREGRTRPP

TAVAHIA
Length:357
Mass (Da):40,181
Last modified:October 1, 1996 - v1
Checksum:iAFAAEFE7152CFE1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71467 Genomic DNA. Translation: CAA96085.1.
AY692717 Genomic DNA. Translation: AAT92736.1.
BK006947 Genomic DNA. Translation: DAA10362.1.
PIRiS63146.
RefSeqiNP_014208.1. NM_001183029.1.

Genome annotation databases

EnsemblFungiiYNL191W; YNL191W; YNL191W.
GeneIDi855530.
KEGGisce:YNL191W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71467 Genomic DNA. Translation: CAA96085.1.
AY692717 Genomic DNA. Translation: AAT92736.1.
BK006947 Genomic DNA. Translation: DAA10362.1.
PIRiS63146.
RefSeqiNP_014208.1. NM_001183029.1.

3D structure databases

ProteinModelPortaliP53871.
SMRiP53871. Positions 46-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35642. 6 interactions.
DIPiDIP-3956N.
IntActiP53871. 4 interactions.
MINTiMINT-501795.

Proteomic databases

MaxQBiP53871.
PaxDbiP53871.
PeptideAtlasiP53871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL191W; YNL191W; YNL191W.
GeneIDi855530.
KEGGisce:YNL191W.

Organism-specific databases

CYGDiYNL191w.
EuPathDBiFungiDB:YNL191W.
SGDiS000005135. DUG3.

Phylogenomic databases

eggNOGiCOG0121.
HOGENOMiHOG000256686.
InParanoidiP53871.
KOiK18802.
OMAiYPHNPRI.
OrthoDBiEOG7QNVX7.

Enzyme and pathway databases

BioCyciYEAST:G3O-33202-MONOMER.

Miscellaneous databases

NextBioi979577.
PROiP53871.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR026869. Put_GATase_2.
[Graphical view]
PfamiPF13230. GATase_4. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae."
    Ganguli D., Kumar C., Bachhawat A.K.
    Genetics 175:1137-1151(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE GSH DEGRADOSOMAL COMPLEX, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDUG3_YEAST
AccessioniPrimary (citable) accession number: P53871
Secondary accession number(s): D6W0Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.