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P53871

- DUG3_YEAST

UniProt

P53871 - DUG3_YEAST

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Protein
Probable glutamine amidotransferase DUG3
Gene
DUG3, YNL191W, N1410
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: SGD
  2. omega peptidase activity Source: SGD
  3. peptidase activity Source: SGD

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. glutathione catabolic process Source: SGD
  3. proteolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamine amidotransferase DUG3
Alternative name(s):
Deficient in utilization of glutathione protein 3
GSH degradosomal complex subunit DUG3
Gene namesi
Name:DUG3
Ordered Locus Names:YNL191W
ORF Names:N1410
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL191w.
SGDiS000005135. DUG3.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 357356Probable glutamine amidotransferase DUG3
PRO_0000203401Add
BLAST

Proteomic databases

MaxQBiP53871.
PaxDbiP53871.
PeptideAtlasiP53871.

Expressioni

Gene expression databases

GenevestigatoriP53871.

Interactioni

Subunit structurei

Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3.1 Publication

Protein-protein interaction databases

BioGridi35642. 5 interactions.
DIPiDIP-3956N.
IntActiP53871. 4 interactions.
MINTiMINT-501795.
STRINGi4932.YNL191W.

Structurei

3D structure databases

ProteinModelPortaliP53871.
SMRiP53871. Positions 46-291.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 260259Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

Belongs to the DUG3 family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0121.
HOGENOMiHOG000256686.
KOiK07008.
OMAiMHNGGIS.
OrthoDBiEOG7QNVX7.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR026869. Put_GATase_2.
[Graphical view]
PfamiPF13230. GATase_4. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53871-1 [UniParc]FASTAAdd to Basket

« Hide

MCRFLIFKGK QPIRLSHLLT RPAHSIINQS FDSRLRLDRR RPMNGDGFGV    50
AYYPLDTELS EDGPCLFKAI TPAWNNQNLS TLAEKTKSDL VFAHVRASTY 100
GVLSETNCHP FTYHSLCFMH NGGISNFKGI KRKLLNHIKD EYLNFIQGST 150
DSECAFALFL DTLDKLGYDP KKQDGDFGNV ALRKAMLRTI DYIRDWTKEA 200
NKDEAHVEPS LLNFAVTDGS TVVVSRYITS KTDEAASLHF SCGSSFVETS 250
PGEYRVERLD RNQDVIMVAS EPLTFERGDW TAVPTNSILT IKKQTILLHP 300
IIDEYYQEDP LYLRSSTLAE SKGLMGSIPL AKAVEKNVPP LEREGRTRPP 350
TAVAHIA 357
Length:357
Mass (Da):40,181
Last modified:October 1, 1996 - v1
Checksum:iAFAAEFE7152CFE1A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71467 Genomic DNA. Translation: CAA96085.1.
AY692717 Genomic DNA. Translation: AAT92736.1.
BK006947 Genomic DNA. Translation: DAA10362.1.
PIRiS63146.
RefSeqiNP_014208.1. NM_001183029.1.

Genome annotation databases

EnsemblFungiiYNL191W; YNL191W; YNL191W.
GeneIDi855530.
KEGGisce:YNL191W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71467 Genomic DNA. Translation: CAA96085.1 .
AY692717 Genomic DNA. Translation: AAT92736.1 .
BK006947 Genomic DNA. Translation: DAA10362.1 .
PIRi S63146.
RefSeqi NP_014208.1. NM_001183029.1.

3D structure databases

ProteinModelPortali P53871.
SMRi P53871. Positions 46-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35642. 5 interactions.
DIPi DIP-3956N.
IntActi P53871. 4 interactions.
MINTi MINT-501795.
STRINGi 4932.YNL191W.

Proteomic databases

MaxQBi P53871.
PaxDbi P53871.
PeptideAtlasi P53871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL191W ; YNL191W ; YNL191W .
GeneIDi 855530.
KEGGi sce:YNL191W.

Organism-specific databases

CYGDi YNL191w.
SGDi S000005135. DUG3.

Phylogenomic databases

eggNOGi COG0121.
HOGENOMi HOG000256686.
KOi K07008.
OMAi MHNGGIS.
OrthoDBi EOG7QNVX7.

Enzyme and pathway databases

BioCyci YEAST:G3O-33202-MONOMER.

Miscellaneous databases

NextBioi 979577.

Gene expression databases

Genevestigatori P53871.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR026869. Put_GATase_2.
[Graphical view ]
Pfami PF13230. GATase_4. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae."
    Ganguli D., Kumar C., Bachhawat A.K.
    Genetics 175:1137-1151(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE GSH DEGRADOSOMAL COMPLEX, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDUG3_YEAST
AccessioniPrimary (citable) accession number: P53871
Secondary accession number(s): D6W0Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7300 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi