P53866 (SQS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SQS1

Alternative name(s):
Squelch of splicing suppression protein 1

Gene names

Name:	SQS1
Ordered Locus Names:	YNL224C
ORF Names:	N1269

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in splicing since overexpression antagonizes the suppression of splicing defects by SPP382 mutants. Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 1440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SQS1 family. Contains 1 G-patch domain. Contains 1 R3H domain.
Sequence caution	The sequence AAA86499.1 differs from that shown. Reason: Frameshift at position 257.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from genetic interaction Ref.7. Source: SGD maturation of SSU-rRNA Inferred from genetic interaction PubMed 19801658PubMed 19927118. Source: SGD positive regulation of ATPase activity Inferred from mutant phenotype PubMed 19927118. Source: SGD positive regulation of helicase activity Inferred from direct assay PubMed 19927118. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 19927118. Source: SGD cytoplasm Inferred from direct assay Ref.5PubMed 22842922. Source: SGD nucleolus Inferred from direct assay PubMed 22842922. Source: SGD preribosome, large subunit precursor Inferred from direct assay PubMed 19927118. Source: SGD preribosome, small subunit precursor Inferred from direct assay PubMed 16227579PubMed 19927118. Source: SGD
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	Protein SQS1		PRO_0000203388
Regions
	Domain	594 – 656	63	R3H
	Domain	720 – 767	48	G-patch
Amino acid modifications
	Modified residue	105	1	Phosphoserine Ref.9 Ref.10
	Modified residue	106	1	Phosphoserine Ref.10
	Modified residue	217	1	Phosphoserine Ref.10
	Modified residue	255	1	Phosphoserine Ref.10
	Modified residue	334	1	Phosphoserine Ref.9 Ref.10
	Modified residue	343	1	Phosphoserine Ref.8 Ref.9 Ref.10
	Modified residue	345	1	Phosphoserine Ref.8 Ref.10
	Modified residue	372	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P53866 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CE5650B8679BF8C0	FASTA	767	86,950
	10 20 30 40 50 60 MAKRHSHYQG SRRRHARGSN SKKAGRGNAK GIQGRKIKKK PTPTNSWHNS SIPLGEGDLD 70 80 90 100 110 120 DVGADFNPGR AFISPKTIED YYFGRDAKSR SMKMGGLRPG NRYDSSTDLQ AGRAAFRKRP 130 140 150 160 170 180 MQFVKAKEVY DPSHNMIQKL RAKNETKNSE EIVEREADVF EEPGKMTSDV EYINNEDSEN 190 200 210 220 230 240 EDDDSQNSPS TDHSLSSNES KVEDGDLFFV DEEAQQSPDL TKIKRVCIEE IARPREVAIE 250 260 270 280 290 300 FDPILTIGKV ELSVSEGNES KEISVDVPNK GNKTYHPFAG YISNVLHGMH TSDSDNDELD 310 320 330 340 350 360 YEIETENNSE PLYESSASSE VDQGFNYVGQ RHNSRADNNL LPSPSPQLTE DIKCLSINGT 370 380 390 400 410 420 KTFEGNNDNL PSPASEELEF GFKEEDFVIN TNDIVVSNIR MGGVDNSYYL RCYRLLGDYD 430 440 450 460 470 480 FHWIDQDLLT DFVVDELGLP EDRLPAYLNF IKNSLIPKIE PAEPTYSDIP ISDSSDEGDS 490 500 510 520 530 540 YEGDSYEDDE DMASSVVHSD IEEGLDDLIA YTLKHDTERF KTFETKSLET KGKGKKKKLL 550 560 570 580 590 600 IDDALALDTE TLETLQSKFS KRIETKAKKR KAKEDFIDQE NRNSNDMLKK YPYGLHIQNI 610 620 630 640 650 660 KDEFESFLSR NNDRLTFPPL DPHGNKTVMK IAKHYNMKSS KIGKANHTSV VVEKIKKTKW 670 680 690 700 710 720 SSPNYSLIDQ LMRQRPVFMR IDIRRPREEQ AAFERTKTIR GKFHVKEGEI VGQNAPEIGN 730 740 750 760 ENIGRRMLEK LGWKSGEGLG IQGNKGISEP IFAKIKKNRS GLRHSES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain." Pandolfo D., de Antoni A., Lanfranchi G., Valle G. Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	Sun Z., Hampsey M. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Inhibition of a spliceosome turnover pathway suppresses splicing defects." Pandit S., Lynn B., Rymond B.C. Proc. Natl. Acad. Sci. U.S.A. 103:13700-13705(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343 AND SER-345, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-334 AND SER-343, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-106; SER-217; SER-255; SER-334; SER-343; SER-345 AND SER-372, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69381 Genomic DNA. Translation: CAA93374.1. Z71500 Genomic DNA. Translation: CAA96127.1. U20390 Genomic DNA. Translation: AAA86499.1. Frameshift. BK006947 Genomic DNA. Translation: DAA10332.1.
PIR	S63182.
RefSeq	NP_014175.1. NM_001183062.1.
3D structure databases
ProteinModelPortal	P53866.
SMR	P53866. Positions 596-656.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6728N.
IntAct	P53866. 7 interactions.
MINT	MINT-618682.
STRING	4932.YNL224C.
Proteomic databases
PaxDb	P53866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL224C; YNL224C; YNL224C.
GeneID	855497.
KEGG	sce:YNL224C.
Organism-specific databases
CYGD	YNL224c.
SGD	S000005168. SQS1.
Phylogenomic databases
eggNOG	NOG114135.
HOGENOM	HOG000154406.
OMA	NIGRRML.
OrthoDB	EOG4V46HJ.
Gene expression databases
Genevestigator	P53866.
GermOnline	YNL224C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000467. G_patch_dom. [Graphical view]
Pfam	PF01585. G-patch. 1 hit. [Graphical view]
SMART	SM00443. G_patch. 1 hit. [Graphical view]
PROSITE	PS50174. G_PATCH. 1 hit. PS51061. R3H. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979487.

Entry information

Entry name

SQS1_YEAST

Accession

Primary (citable) accession number: P53866
Secondary accession number(s): D6W0W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program