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P53866

- SQS1_YEAST

UniProt

P53866 - SQS1_YEAST

Protein

Protein SQS1

Gene

SQS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May be involved in splicing since overexpression antagonizes the suppression of splicing defects by SPP382 mutants.1 Publication

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. maturation of SSU-rRNA Source: SGD
    2. mRNA splicing, via spliceosome Source: SGD
    3. positive regulation of ATPase activity Source: SGD
    4. positive regulation of helicase activity Source: SGD
    5. rRNA processing Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33227-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SQS1
    Alternative name(s):
    Squelch of splicing suppression protein 1
    Gene namesi
    Name:SQS1
    Ordered Locus Names:YNL224C
    ORF Names:N1269
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL224c.
    SGDiS000005168. SQS1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. 90S preribosome Source: SGD
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell
    4. preribosome, large subunit precursor Source: SGD
    5. preribosome, small subunit precursor Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 767767Protein SQS1PRO_0000203388Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei105 – 1051Phosphoserine1 Publication
    Modified residuei217 – 2171Phosphoserine1 Publication
    Modified residuei255 – 2551Phosphoserine1 Publication
    Modified residuei334 – 3341Phosphoserine1 Publication
    Modified residuei343 – 3431Phosphoserine2 Publications
    Modified residuei345 – 3451Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53866.
    PaxDbiP53866.

    Expressioni

    Gene expression databases

    GenevestigatoriP53866.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP43P531317EBI-29168,EBI-505

    Protein-protein interaction databases

    BioGridi35612. 65 interactions.
    DIPiDIP-6728N.
    IntActiP53866. 7 interactions.
    MINTiMINT-618682.
    STRINGi4932.YNL224C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53866.
    SMRiP53866. Positions 596-656.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini594 – 65663R3HAdd
    BLAST
    Domaini720 – 76748G-patchPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SQS1 family.Curated
    Contains 1 G-patch domain.PROSITE-ProRule annotation
    Contains 1 R3H domain.Curated

    Phylogenomic databases

    eggNOGiNOG114135.
    HOGENOMiHOG000154406.
    OMAiNIGRRML.
    OrthoDBiEOG7ZGXCD.

    Family and domain databases

    Gene3Di3.30.1370.50. 1 hit.
    InterProiIPR000467. G_patch_dom.
    IPR001374. R3H_ss-bd.
    [Graphical view]
    PfamiPF01585. G-patch. 1 hit.
    [Graphical view]
    SMARTiSM00443. G_patch. 1 hit.
    [Graphical view]
    SUPFAMiSSF82708. SSF82708. 1 hit.
    PROSITEiPS50174. G_PATCH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53866-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKRHSHYQG SRRRHARGSN SKKAGRGNAK GIQGRKIKKK PTPTNSWHNS    50
    SIPLGEGDLD DVGADFNPGR AFISPKTIED YYFGRDAKSR SMKMGGLRPG 100
    NRYDSSTDLQ AGRAAFRKRP MQFVKAKEVY DPSHNMIQKL RAKNETKNSE 150
    EIVEREADVF EEPGKMTSDV EYINNEDSEN EDDDSQNSPS TDHSLSSNES 200
    KVEDGDLFFV DEEAQQSPDL TKIKRVCIEE IARPREVAIE FDPILTIGKV 250
    ELSVSEGNES KEISVDVPNK GNKTYHPFAG YISNVLHGMH TSDSDNDELD 300
    YEIETENNSE PLYESSASSE VDQGFNYVGQ RHNSRADNNL LPSPSPQLTE 350
    DIKCLSINGT KTFEGNNDNL PSPASEELEF GFKEEDFVIN TNDIVVSNIR 400
    MGGVDNSYYL RCYRLLGDYD FHWIDQDLLT DFVVDELGLP EDRLPAYLNF 450
    IKNSLIPKIE PAEPTYSDIP ISDSSDEGDS YEGDSYEDDE DMASSVVHSD 500
    IEEGLDDLIA YTLKHDTERF KTFETKSLET KGKGKKKKLL IDDALALDTE 550
    TLETLQSKFS KRIETKAKKR KAKEDFIDQE NRNSNDMLKK YPYGLHIQNI 600
    KDEFESFLSR NNDRLTFPPL DPHGNKTVMK IAKHYNMKSS KIGKANHTSV 650
    VVEKIKKTKW SSPNYSLIDQ LMRQRPVFMR IDIRRPREEQ AAFERTKTIR 700
    GKFHVKEGEI VGQNAPEIGN ENIGRRMLEK LGWKSGEGLG IQGNKGISEP 750
    IFAKIKKNRS GLRHSES 767
    Length:767
    Mass (Da):86,950
    Last modified:October 1, 1996 - v1
    Checksum:iCE5650B8679BF8C0
    GO

    Sequence cautioni

    The sequence AAA86499.1 differs from that shown. Reason: Frameshift at position 257.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69381 Genomic DNA. Translation: CAA93374.1.
    Z71500 Genomic DNA. Translation: CAA96127.1.
    U20390 Genomic DNA. Translation: AAA86499.1. Frameshift.
    BK006947 Genomic DNA. Translation: DAA10332.1.
    PIRiS63182.
    RefSeqiNP_014175.1. NM_001183062.1.

    Genome annotation databases

    EnsemblFungiiYNL224C; YNL224C; YNL224C.
    GeneIDi855497.
    KEGGisce:YNL224C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69381 Genomic DNA. Translation: CAA93374.1 .
    Z71500 Genomic DNA. Translation: CAA96127.1 .
    U20390 Genomic DNA. Translation: AAA86499.1 . Frameshift.
    BK006947 Genomic DNA. Translation: DAA10332.1 .
    PIRi S63182.
    RefSeqi NP_014175.1. NM_001183062.1.

    3D structure databases

    ProteinModelPortali P53866.
    SMRi P53866. Positions 596-656.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35612. 65 interactions.
    DIPi DIP-6728N.
    IntActi P53866. 7 interactions.
    MINTi MINT-618682.
    STRINGi 4932.YNL224C.

    Proteomic databases

    MaxQBi P53866.
    PaxDbi P53866.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL224C ; YNL224C ; YNL224C .
    GeneIDi 855497.
    KEGGi sce:YNL224C.

    Organism-specific databases

    CYGDi YNL224c.
    SGDi S000005168. SQS1.

    Phylogenomic databases

    eggNOGi NOG114135.
    HOGENOMi HOG000154406.
    OMAi NIGRRML.
    OrthoDBi EOG7ZGXCD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33227-MONOMER.

    Miscellaneous databases

    NextBioi 979487.

    Gene expression databases

    Genevestigatori P53866.

    Family and domain databases

    Gene3Di 3.30.1370.50. 1 hit.
    InterProi IPR000467. G_patch_dom.
    IPR001374. R3H_ss-bd.
    [Graphical view ]
    Pfami PF01585. G-patch. 1 hit.
    [Graphical view ]
    SMARTi SM00443. G_patch. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82708. SSF82708. 1 hit.
    PROSITEi PS50174. G_PATCH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
      Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
      Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Sun Z., Hampsey M.
      Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Inhibition of a spliceosome turnover pathway suppresses splicing defects."
      Pandit S., Lynn B., Rymond B.C.
      Proc. Natl. Acad. Sci. U.S.A. 103:13700-13705(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217; SER-255 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-334; SER-343 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSQS1_YEAST
    AccessioniPrimary (citable) accession number: P53866
    Secondary accession number(s): D6W0W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1440 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3