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P53866 (SQS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SQS1
Alternative name(s):
Squelch of splicing suppression protein 1
Gene names
Name:SQS1
Ordered Locus Names:YNL224C
ORF Names:N1269
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length767 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in splicing since overexpression antagonizes the suppression of splicing defects by SPP382 mutants. Ref.7

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 1440 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SQS1 family.

Contains 1 G-patch domain.

Contains 1 R3H domain.

Sequence caution

The sequence AAA86499.1 differs from that shown. Reason: Frameshift at position 257.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP43P531317EBI-29168,EBI-505

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 767767Protein SQS1
PRO_0000203388

Regions

Domain594 – 65663R3H
Domain720 – 76748G-patch

Amino acid modifications

Modified residue1051Phosphoserine Ref.10
Modified residue2171Phosphoserine Ref.9
Modified residue2551Phosphoserine Ref.9
Modified residue3341Phosphoserine Ref.10
Modified residue3431Phosphoserine Ref.8 Ref.10
Modified residue3451Phosphoserine Ref.8 Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P53866 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: CE5650B8679BF8C0

FASTA76786,950
        10         20         30         40         50         60 
MAKRHSHYQG SRRRHARGSN SKKAGRGNAK GIQGRKIKKK PTPTNSWHNS SIPLGEGDLD 

        70         80         90        100        110        120 
DVGADFNPGR AFISPKTIED YYFGRDAKSR SMKMGGLRPG NRYDSSTDLQ AGRAAFRKRP 

       130        140        150        160        170        180 
MQFVKAKEVY DPSHNMIQKL RAKNETKNSE EIVEREADVF EEPGKMTSDV EYINNEDSEN 

       190        200        210        220        230        240 
EDDDSQNSPS TDHSLSSNES KVEDGDLFFV DEEAQQSPDL TKIKRVCIEE IARPREVAIE 

       250        260        270        280        290        300 
FDPILTIGKV ELSVSEGNES KEISVDVPNK GNKTYHPFAG YISNVLHGMH TSDSDNDELD 

       310        320        330        340        350        360 
YEIETENNSE PLYESSASSE VDQGFNYVGQ RHNSRADNNL LPSPSPQLTE DIKCLSINGT 

       370        380        390        400        410        420 
KTFEGNNDNL PSPASEELEF GFKEEDFVIN TNDIVVSNIR MGGVDNSYYL RCYRLLGDYD 

       430        440        450        460        470        480 
FHWIDQDLLT DFVVDELGLP EDRLPAYLNF IKNSLIPKIE PAEPTYSDIP ISDSSDEGDS 

       490        500        510        520        530        540 
YEGDSYEDDE DMASSVVHSD IEEGLDDLIA YTLKHDTERF KTFETKSLET KGKGKKKKLL 

       550        560        570        580        590        600 
IDDALALDTE TLETLQSKFS KRIETKAKKR KAKEDFIDQE NRNSNDMLKK YPYGLHIQNI 

       610        620        630        640        650        660 
KDEFESFLSR NNDRLTFPPL DPHGNKTVMK IAKHYNMKSS KIGKANHTSV VVEKIKKTKW 

       670        680        690        700        710        720 
SSPNYSLIDQ LMRQRPVFMR IDIRRPREEQ AAFERTKTIR GKFHVKEGEI VGQNAPEIGN 

       730        740        750        760 
ENIGRRMLEK LGWKSGEGLG IQGNKGISEP IFAKIKKNRS GLRHSES 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]Sun Z., Hampsey M.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-298.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Inhibition of a spliceosome turnover pathway suppresses splicing defects."
Pandit S., Lynn B., Rymond B.C.
Proc. Natl. Acad. Sci. U.S.A. 103:13700-13705(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-343 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217; SER-255 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-334; SER-343 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z69381 Genomic DNA. Translation: CAA93374.1.
Z71500 Genomic DNA. Translation: CAA96127.1.
U20390 Genomic DNA. Translation: AAA86499.1. Frameshift.
BK006947 Genomic DNA. Translation: DAA10332.1.
PIRS63182.
RefSeqNP_014175.1. NM_001183062.1.

3D structure databases

ProteinModelPortalP53866.
SMRP53866. Positions 596-656.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35612. 65 interactions.
DIPDIP-6728N.
IntActP53866. 7 interactions.
MINTMINT-618682.
STRING4932.YNL224C.

Proteomic databases

MaxQBP53866.
PaxDbP53866.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL224C; YNL224C; YNL224C.
GeneID855497.
KEGGsce:YNL224C.

Organism-specific databases

CYGDYNL224c.
SGDS000005168. SQS1.

Phylogenomic databases

eggNOGNOG114135.
HOGENOMHOG000154406.
OMANIGRRML.
OrthoDBEOG7ZGXCD.

Enzyme and pathway databases

BioCycYEAST:G3O-33227-MONOMER.

Gene expression databases

GenevestigatorP53866.

Family and domain databases

Gene3D3.30.1370.50. 1 hit.
InterProIPR000467. G_patch_dom.
IPR001374. R3H_ss-bd.
[Graphical view]
PfamPF01585. G-patch. 1 hit.
[Graphical view]
SMARTSM00443. G_patch. 1 hit.
[Graphical view]
SUPFAMSSF82708. SSF82708. 1 hit.
PROSITEPS50174. G_PATCH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979487.

Entry information

Entry nameSQS1_YEAST
AccessionPrimary (citable) accession number: P53866
Secondary accession number(s): D6W0W6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families