P53863 (JJJ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
J protein JJJ1

Gene names

Name:	JJJ1
Ordered Locus Names:	YNL227C
ORF Names:	N1254

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus Potential.
Miscellaneous	Present with 2310 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 C2H2-type zinc fingers. Contains 1 J domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from mutant phenotype PubMed 11378903. Source: SGD rRNA processing Inferred from mutant phenotype PubMed 20368619. Source: SGD regulation of cell size Inferred from mutant phenotype PubMed 12089449. Source: SGD ribosomal large subunit biogenesis Inferred from mutant phenotype PubMed 17242366. Source: SGD ribosomal subunit export from nucleus Inferred from mutant phenotype PubMed 20368619. Source: SGD
Cellular_component	cytosol Inferred from direct assay PubMed 17242366. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD nucleolus Inferred from mutant phenotype PubMed 20368619. Source: SGD
Molecular_function	ATPase activator activity Inferred from direct assay PubMed 17242366. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	J protein JJJ1		PRO_0000071162
Regions
	Domain	3 – 72	70	J
	Zinc finger	338 – 362	25	C2H2-type 1
	Zinc finger	549 – 573	25	C2H2-type 2
Amino acid modifications
	Modified residue	504	1	Phosphothreonine Ref.5 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P53863 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0D184EF861105C95	FASTA	590	68,783
	10 20 30 40 50 60 MKTCYYELLG VETHASDLEL KKAYRKKALQ YHPDKNPDNV EEATQKFAVI RAAYEVLSDP 70 80 90 100 110 120 QERAWYDSHK EQILNDTPPS TDDYYDYEVD ATVTGVTTDE LLLFFNSALY TKIDNSAAGI 130 140 150 160 170 180 YQIAGKIFAK LAKDEILSGK RLGKFSEYQD DVFEQDINSI GYLKACDNFI NKTDKLLYPL 190 200 210 220 230 240 FGYSPTDYEY LKHFYKTWSA FNTLKSFSWK DEYMYSKNYD RRTKREVNRR NEKARQQARN 250 260 270 280 290 300 EYNKTVKRFV VFIKKLDKRM KEGAKIAEEQ RKLKEQQRKN ELNNRRKFGN DNNDEEKFHL 310 320 330 340 350 360 QSWQTVKEEN WDELEKVYDN FGEFENSKND KEGEVLIYEC FICNKTFKSE KQLKNHINTK 370 380 390 400 410 420 LHKKNMEEIR KEMEEENITL GLDNLSDLEK FDSADESVKE KEDIDLQALQ AELAEIERKL 430 440 450 460 470 480 AESSSEDESE DDNLNIEMDI EVEDVSSDEN VHVNTKNKKK RKKKKKAKVD TETEESESFD 490 500 510 520 530 540 DTKDKRSNEL DDLLASLGDK GLQTDDDEDW STKAKKKKGK QPKKNSKSTK STPSLSTLPS 550 560 570 580 590 SMSPTSAIEV CTTCGESFDS RNKLFNHVKI AGHAAVKNVV KRKKVKTKRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain." Pandolfo D., de Antoni A., Lanfranchi G., Valle G. Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-504, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-504, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69381 Genomic DNA. Translation: CAA93371.1. Z71504 Genomic DNA. Translation: CAA96132.1. BK006947 Genomic DNA. Translation: DAA10330.1.
PIR	S63193.
RefSeq	NP_014172.1. NM_001183065.1.
3D structure databases
ProteinModelPortal	P53863.
SMR	P53863. Positions 5-67.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-972N.
IntAct	P53863. 22 interactions.
MINT	MINT-424101.
STRING	4932.YNL227C.
Proteomic databases
PaxDb	P53863.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL227C; YNL227C; YNL227C.
GeneID	855495.
KEGG	sce:YNL227C.
Organism-specific databases
CYGD	YNL227c.
SGD	S000005171. JJJ1.
Phylogenomic databases
eggNOG	COG2214.
GeneTree	ENSGT00510000047097.
HOGENOM	HOG000193307.
KO	K09506.
OMA	EKQLENH.
OrthoDB	EOG4MWCG2.
Gene expression databases
Genevestigator	P53863.
GermOnline	YNL227C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR001623. DnaJ_domain. IPR018253. DnaJ_domain_CS. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR022755. Znf_C2H2_jaz. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF12171. zf-C2H2_jaz. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. SM00355. ZnF_C2H2. 2 hits. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	979481.

Entry information

Entry name

JJJ1_YEAST

Accession

Primary (citable) accession number: P53863
Secondary accession number(s): D6W0W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program