P53862 (YNW8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
putative uncharacterized membrane protein YNL228W

Gene names

Ordered Locus Names:	YNL228W
ORF Names:	N1249

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Caution	Product of a dubious gene prediction. Almost completely overlaps JJJ1.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 258	235	putative uncharacterized membrane protein YNL228W		PRO_0000014339
Regions
	Topological domain	24 – 64	41	Cytoplasmic Potential
	Transmembrane	65 – 85	21	Helical; Potential
	Topological domain	86 – 123	38	Extracellular Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Topological domain	145 – 230	86	Cytoplasmic Potential
	Transmembrane	231 – 251	21	Helical; Potential
	Topological domain	252 – 258	7	Extracellular Potential
	Compositional bias	79 – 82	4	Poly-Phe
	Compositional bias	88 – 92	5	Poly-Ser
	Compositional bias	129 – 141	13	Poly-Phe
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53862 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 24F58C70CD10E86C	FASTA	258	28,642
	10 20 30 40 50 60 MVWCHYILLV LTFFLFTTFF TAACPAIFTW LNSLFRLSND SPHVVHTSIA EVGDIEDGRV 70 80 90 100 110 120 DKDGVLFVDL EFFLGCLPFF FFALVDQSSS SSVCKPLSPS DAKRSSNSLL RLSLVSSNDS 130 140 150 160 170 180 DSSVSVSTFA FFFFFLFFLF FVFTCTFSSE LTSSTSISIS MLRLSSSLSS SEDDSASFLS 190 200 210 220 230 240 ISASSACNAC RSISSFSLTL SSAESNFSRS ERLSNPSVMF SSSISFRISS IFFLCSLVFM 250 WFFNCFSDLN VLLQIKHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain." Pandolfo D., de Antoni A., Lanfranchi G., Valle G. Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69381 Genomic DNA. Translation: CAA93370.1. Z71504 Genomic DNA. Translation: CAA96131.1.
PIR	S63194.
3D structure databases
ProteinModelPortal	P53862.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4282N.
MINT	MINT-558266.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL228W; YNL228W; YNL228W.
Organism-specific databases
SGD	S000005172. YNL228W.
Gene expression databases
Genevestigator	P53862.
GermOnline	YNL228W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

YNW8_YEAST

Accession

Primary (citable) accession number: P53862

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program