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P53855

- ATG2_YEAST

UniProt

P53855 - ATG2_YEAST

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Protein

Autophagy-related protein 2

Gene

ATG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for cytoplasm to vacuole transport (Cvt) vesicle and autophagosome completion. Necessary for the localization of ATG18 to the preautophagosomal structure (PAS) and the binding of ATG18 to ATG9. Involved in correct ATG9 trafficking through the preautophagosomal structure and in peroxisome degradation. ATG2 is the most downstream ATG protein in the preautophagosomal structure organization process. Plays a significant role in life span extension.14 Publications

GO - Biological processi

  1. autophagic vacuole assembly Source: SGD
  2. autophagy Source: UniProtKB
  3. CVT pathway Source: SGD
  4. late nucleophagy Source: SGD
  5. mitochondrion degradation Source: SGD
  6. peroxisome degradation Source: SGD
  7. piecemeal microautophagy of nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33239-MONOMER.

Protein family/group databases

TCDBi9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 2
Alternative name(s):
Sporulation-specific protein 72
Gene namesi
Name:ATG2
Synonyms:APG2, AUT8, SPO72
Ordered Locus Names:YNL242W
ORF Names:N1106
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL242w.
SGDiS000005186. ATG2.

Subcellular locationi

Preautophagosomal structure membrane 7 Publications; Peripheral membrane protein 7 Publications
Note: ATG1 and ATG9 are required for the proper localization the PAS.

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. extrinsic component of membrane Source: SGD
  3. extrinsic component of pre-autophagosomal structure membrane Source: UniProtKB
  4. pre-autophagosomal structure Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi83 – 831G → E: Leads to a severely reduced activity of autophagy and a dispersed localization in the cytoplasm. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15921592Autophagy-related protein 2PRO_0000215836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53855.
PaxDbiP53855.

Expressioni

Gene expression databases

GenevestigatoriP53855.

Interactioni

Subunit structurei

Interacts with ATG18.4 Publications

Protein-protein interaction databases

BioGridi35597. 18 interactions.
DIPiDIP-2620N.
IntActiP53855. 4 interactions.
MINTiMINT-426483.
STRINGi4932.YNL242W.

Structurei

3D structure databases

ProteinModelPortaliP53855.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1049 – 107527Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the ATG2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG304940.
GeneTreeiENSGT00620000087966.
HOGENOMiHOG000034152.
InParanoidiP53855.
KOiK17906.
OMAiMSSWLPQ.
OrthoDBiEOG7B31W6.

Family and domain databases

InterProiIPR026849. ATG2.
IPR026885. ATG2_CAD_motif.
IPR015412. Autophagy-rel_C.
[Graphical view]
PANTHERiPTHR13190. PTHR13190. 1 hit.
PfamiPF13329. ATG2_CAD. 1 hit.
PF09333. ATG_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53855-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFWLPQNIQ KRLLLYVLQQ ISLFSNIDLS NLDVSIGSKS HFSFHDVNLS
60 70 80 90 100
LDDLNIPNVQ INEGIVDELV LKLTVSGGVE IDGSGLRFIM TPLYSSGSQE
110 120 130 140 150
LHSDFLVKSI QDLTNSMLQF SDPLTTYNRY KEDDISSSDS SSDLNSNIEA
160 170 180 190 200
SKPAANGSYT LQNMRNKALN VALAKLKIAL KDVTIRFIVN DRDPSDNIVE
210 220 230 240 250
VHLESIQLIT TDANLRHINI ENITISSIQK QAVPDSPVHP FNNDDLSQSV
260 270 280 290 300
YLSKMEATSL YMSAMEEQSN EDPSEPQVTQ EEQENDKCKE SLMEINNLNI
310 320 330 340 350
AFKGLSSVND LRMSNIVIDI QDVHLAIHKI VEIKNSTLKN IIDIIVTHLD
360 370 380 390 400
ANESFSCQDS QSPSPDKQEP SALSSVDIKC IYLNLGQDIT VILKSFKLEQ
410 420 430 440 450
KENNSLAFSL GSFYSNSSPL TISHKTKPLL TGEQTPQSIA LNMGDELDII
460 470 480 490 500
ISHDGIAHFF KIFQFVSKCM SFYQNKSKGM MPQIASDTKR TVQLTSKAVK
510 520 530 540 550
LSLKFPYFLL CFQVSPFIYD SNRELYIELV DVFKKLPSRC TKILTMSSIT
560 570 580 590 600
ISNLQSPLQL GSYDDTLKEA LIYSSVHAII KEVIFNEEYS GIVQLVEDIS
610 620 630 640 650
AFGKLFTDSK NSECTGKSKS KRGSFLQRSV RVLNSSRFVY KQSLSANFSL
660 670 680 690 700
KIDSMKLKVS EIIGPQFGSV EALLSNNFFA ITDDSQIVYF TKNLKVERKT
710 720 730 740 750
PSLLEPQEIM SVVLNKAVNE PVLYVHRRAN GKLKVIFNNI RIHYYARWLE
760 770 780 790 800
ILKKNIGPDN ASSKDEPVSQ KLSKKQPTSG FPWELKCLDC SLILHPFRLK
810 820 830 840 850
SVMVIVLDNL TTGGSSFIPQ AKLLSKANTL FLIDDYQNFK IQKDKNWPSL
860 870 880 890 900
INFYAGQGFS AIGKIDTLNF LINKSDGALL LDCKIEQVGL SLCADSFQTF
910 920 930 940 950
CQLCIDLKYP QTFPDEEKFR TQLKNPIDVF KDIDCDLFNS AFIRENNHQN
960 970 980 990 1000
DYDSVHLVDS FLDKTHEFNN GARSKLSSQG SYEMDSSSGT ATGGILLPHE
1010 1020 1030 1040 1050
SYLDSAQPKE EDTPPIASKE QERDVDIRGS IDVEKVVIKL FDGYDWKYTR
1060 1070 1080 1090 1100
KFIANTVEKL DKELSKAEAS SSKSNVPQSE ANIFDSIYIS ANKNNVTDLR
1110 1120 1130 1140 1150
RNLDGEIQGV QNSFSDVSKV NLRPSKHYKA LIQLNKVHVN LKNYRVDEPD
1160 1170 1180 1190 1200
ESNSDNSTDV LNRCVVSIYE FEIIDNVPTS TWNKFVTLLK HEPWPHSSPM
1210 1220 1230 1240 1250
FLLDLEFIRP IDFLQAVELV MQLNVAPLRL HVDQDTLEFL IRFLGFKDKR
1260 1270 1280 1290 1300
FELIDEYPDI VFIQKFSTNS IKLRLDYKPK KVDYAGLRSG QTSELMNFFT
1310 1320 1330 1340 1350
LDGSKIILKS VVLYGLNGFD ELNNKLKAIW TPDITKKQLP GVLEGLAPVR
1360 1370 1380 1390 1400
SFMAIGSGVK TLVTVLMSEY RQEGHLGRSL KKGGNVFLKT TTGDFVKLGV
1410 1420 1430 1440 1450
KLTSGTQAIL ENTEELFGGV GSNGRVYDAS KFGSADGADS DTAAVLDLDT
1460 1470 1480 1490 1500
LFEEDQLVGS KYSRIRDHEP TAVVIDMSSP GDHNEPTIVS LYADQPLDLP
1510 1520 1530 1540 1550
TGLKEAYSSL EKHMHIAYDA VWRAKGQMKD DKRGGPSAAA VYVARAAPVA
1560 1570 1580 1590
IIRPLIGATE AVSKTLQGIA NQVDKTHNEQ INDKYKSNRT DS
Length:1,592
Mass (Da):178,414
Last modified:October 1, 1996 - v1
Checksum:i54FDDF56297FFF99
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z69381 Genomic DNA. Translation: CAA93356.1.
Z71518 Genomic DNA. Translation: CAA96147.1.
BK006947 Genomic DNA. Translation: DAA10317.1.
PIRiS63208.
RefSeqiNP_014157.1. NM_001183080.1.

Genome annotation databases

EnsemblFungiiYNL242W; YNL242W; YNL242W.
GeneIDi855479.
KEGGisce:YNL242W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z69381 Genomic DNA. Translation: CAA93356.1 .
Z71518 Genomic DNA. Translation: CAA96147.1 .
BK006947 Genomic DNA. Translation: DAA10317.1 .
PIRi S63208.
RefSeqi NP_014157.1. NM_001183080.1.

3D structure databases

ProteinModelPortali P53855.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35597. 18 interactions.
DIPi DIP-2620N.
IntActi P53855. 4 interactions.
MINTi MINT-426483.
STRINGi 4932.YNL242W.

Protein family/group databases

TCDBi 9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Proteomic databases

MaxQBi P53855.
PaxDbi P53855.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL242W ; YNL242W ; YNL242W .
GeneIDi 855479.
KEGGi sce:YNL242W.

Organism-specific databases

CYGDi YNL242w.
SGDi S000005186. ATG2.

Phylogenomic databases

eggNOGi NOG304940.
GeneTreei ENSGT00620000087966.
HOGENOMi HOG000034152.
InParanoidi P53855.
KOi K17906.
OMAi MSSWLPQ.
OrthoDBi EOG7B31W6.

Enzyme and pathway databases

BioCyci YEAST:G3O-33239-MONOMER.

Miscellaneous databases

NextBioi 979442.

Gene expression databases

Genevestigatori P53855.

Family and domain databases

InterProi IPR026849. ATG2.
IPR026885. ATG2_CAD_motif.
IPR015412. Autophagy-rel_C.
[Graphical view ]
PANTHERi PTHR13190. PTHR13190. 1 hit.
Pfami PF13329. ATG2_CAD. 1 hit.
PF09333. ATG_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
    Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
    Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
    Tsukada M., Ohsumi Y.
    FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Disruption of six unknown open reading frames from Saccharomyces cerevisiae reveals two genes involved in vacuolar morphogenesis and one gene required for sporulation."
    Saiz J.E., de Los Angeles Santos M., Vazquez de Aldana C.R., Revuelta J.L.
    Yeast 15:155-164(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A genomic screen identifies AUT8 as a novel gene essential for autophagy in the yeast Saccharomyces cerevisiae."
    Barth H., Thumm M.
    Gene 274:151-156(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways."
    Wang C.-W., Kim J., Huang W.-P., Abeliovich H., Stromhaug P.E., Dunn W.A. Jr., Klionsky D.J.
    J. Biol. Chem. 276:30442-30451(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Apg2p functions in autophagosome formation on the perivacuolar structure."
    Shintani T., Suzuki K., Kamada Y., Noda T., Ohsumi Y.
    J. Biol. Chem. 276:30452-30460(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-83.
  9. Cited for: NOMENCLATURE.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure."
    Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J.
    Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
    Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
    Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG18.
  13. "The Atg18-Atg2 complex is recruited to autophagic membranes via phosphatidylinositol 3-phosphate and exerts an essential function."
    Obara K., Sekito T., Niimi K., Ohsumi Y.
    J. Biol. Chem. 283:23972-23980(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG18, SUBCELLULAR LOCATION.
  14. "Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy."
    Geng J., Baba M., Nair U., Klionsky D.J.
    J. Cell Biol. 182:129-140(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy."
    He C., Baba M., Cao Y., Klionsky D.J.
    Mol. Biol. Cell 19:5506-5516(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Atg17 recruits Atg9 to organize the pre-autophagosomal structure."
    Sekito T., Kawamata T., Ichikawa R., Suzuki K., Ohsumi Y.
    Genes Cells 14:525-538(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  19. "Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae."
    van der Vaart A., Griffith J., Reggiori F.
    Mol. Biol. Cell 21:2270-2284(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  20. "Tor directly controls the Atg1 kinase complex to regulate autophagy."
    Kamada Y., Yoshino K., Kondo C., Kawamata T., Oshiro N., Yonezawa K., Ohsumi Y.
    Mol. Cell. Biol. 30:1049-1058(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  21. "Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2."
    Kobayashi T., Suzuki K., Ohsumi Y.
    FEBS Lett. 586:2473-2478(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  22. "Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18."
    Watanabe Y., Kobayashi T., Yamamoto H., Hoshida H., Akada R., Inagaki F., Ohsumi Y., Noda N.N.
    J. Biol. Chem. 287:31681-31690(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG18.
  23. "Atg18 function in autophagy is regulated by specific sites within its beta-propeller."
    Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F.
    J. Cell Sci. 126:593-604(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG18, FUNCTION.
  24. "Autophagy and leucine promote chronological longevity and respiration proficiency during calorie restriction in yeast."
    Aris J.P., Alvers A.L., Ferraiuolo R.A., Fishwick L.K., Hanvivatpong A., Hu D., Kirlew C., Leonard M.T., Losin K.J., Marraffini M., Seo A.Y., Swanberg V., Westcott J.L., Wood M.S., Leeuwenburgh C., Dunn W.A. Jr.
    Exp. Gerontol. 48:1107-1119(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiATG2_YEAST
AccessioniPrimary (citable) accession number: P53855
Secondary accession number(s): D6W0V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3