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P53855

- ATG2_YEAST

UniProt

P53855 - ATG2_YEAST

Protein

Autophagy-related protein 2

Gene

ATG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required for cytoplasm to vacuole transport (Cvt) vesicle and autophagosome completion. Necessary for the localization of ATG18 to the preautophagosomal structure (PAS) and the binding of ATG18 to ATG9. Involved in correct ATG9 trafficking through the preautophagosomal structure and in peroxisome degradation. ATG2 is the most downstream ATG protein in the preautophagosomal structure organization process. Plays a significant role in life span extension.14 Publications

    GO - Biological processi

    1. autophagic vacuole assembly Source: SGD
    2. autophagy Source: UniProtKB
    3. CVT pathway Source: SGD
    4. late nucleophagy Source: SGD
    5. mitochondrion degradation Source: SGD
    6. peroxisome degradation Source: SGD
    7. piecemeal microautophagy of nucleus Source: SGD

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33239-MONOMER.

    Protein family/group databases

    TCDBi9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autophagy-related protein 2
    Alternative name(s):
    Sporulation-specific protein 72
    Gene namesi
    Name:ATG2
    Synonyms:APG2, AUT8, SPO72
    Ordered Locus Names:YNL242W
    ORF Names:N1106
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL242w.
    SGDiS000005186. ATG2.

    Subcellular locationi

    Preautophagosomal structure membrane 7 Publications; Peripheral membrane protein 7 Publications
    Note: ATG1 and ATG9 are required for the proper localization the PAS.

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. extrinsic component of membrane Source: SGD
    3. pre-autophagosomal structure Source: SGD
    4. pre-autophagosomal structure membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi83 – 831G → E: Leads to a severely reduced activity of autophagy and a dispersed localization in the cytoplasm. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15921592Autophagy-related protein 2PRO_0000215836Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei236 – 2361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53855.
    PaxDbiP53855.

    Expressioni

    Gene expression databases

    GenevestigatoriP53855.

    Interactioni

    Subunit structurei

    Interacts with ATG18.4 Publications

    Protein-protein interaction databases

    BioGridi35597. 18 interactions.
    DIPiDIP-2620N.
    IntActiP53855. 4 interactions.
    MINTiMINT-426483.
    STRINGi4932.YNL242W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53855.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1049 – 107527Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATG2 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG304940.
    GeneTreeiENSGT00620000087966.
    HOGENOMiHOG000034152.
    KOiK17906.
    OMAiMSSWLPQ.
    OrthoDBiEOG7B31W6.

    Family and domain databases

    InterProiIPR026849. ATG2.
    IPR026885. ATG2_CAD_motif.
    IPR015412. Autophagy-rel_C.
    [Graphical view]
    PANTHERiPTHR13190. PTHR13190. 1 hit.
    PfamiPF13329. ATG2_CAD. 1 hit.
    PF09333. ATG_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53855-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFWLPQNIQ KRLLLYVLQQ ISLFSNIDLS NLDVSIGSKS HFSFHDVNLS     50
    LDDLNIPNVQ INEGIVDELV LKLTVSGGVE IDGSGLRFIM TPLYSSGSQE 100
    LHSDFLVKSI QDLTNSMLQF SDPLTTYNRY KEDDISSSDS SSDLNSNIEA 150
    SKPAANGSYT LQNMRNKALN VALAKLKIAL KDVTIRFIVN DRDPSDNIVE 200
    VHLESIQLIT TDANLRHINI ENITISSIQK QAVPDSPVHP FNNDDLSQSV 250
    YLSKMEATSL YMSAMEEQSN EDPSEPQVTQ EEQENDKCKE SLMEINNLNI 300
    AFKGLSSVND LRMSNIVIDI QDVHLAIHKI VEIKNSTLKN IIDIIVTHLD 350
    ANESFSCQDS QSPSPDKQEP SALSSVDIKC IYLNLGQDIT VILKSFKLEQ 400
    KENNSLAFSL GSFYSNSSPL TISHKTKPLL TGEQTPQSIA LNMGDELDII 450
    ISHDGIAHFF KIFQFVSKCM SFYQNKSKGM MPQIASDTKR TVQLTSKAVK 500
    LSLKFPYFLL CFQVSPFIYD SNRELYIELV DVFKKLPSRC TKILTMSSIT 550
    ISNLQSPLQL GSYDDTLKEA LIYSSVHAII KEVIFNEEYS GIVQLVEDIS 600
    AFGKLFTDSK NSECTGKSKS KRGSFLQRSV RVLNSSRFVY KQSLSANFSL 650
    KIDSMKLKVS EIIGPQFGSV EALLSNNFFA ITDDSQIVYF TKNLKVERKT 700
    PSLLEPQEIM SVVLNKAVNE PVLYVHRRAN GKLKVIFNNI RIHYYARWLE 750
    ILKKNIGPDN ASSKDEPVSQ KLSKKQPTSG FPWELKCLDC SLILHPFRLK 800
    SVMVIVLDNL TTGGSSFIPQ AKLLSKANTL FLIDDYQNFK IQKDKNWPSL 850
    INFYAGQGFS AIGKIDTLNF LINKSDGALL LDCKIEQVGL SLCADSFQTF 900
    CQLCIDLKYP QTFPDEEKFR TQLKNPIDVF KDIDCDLFNS AFIRENNHQN 950
    DYDSVHLVDS FLDKTHEFNN GARSKLSSQG SYEMDSSSGT ATGGILLPHE 1000
    SYLDSAQPKE EDTPPIASKE QERDVDIRGS IDVEKVVIKL FDGYDWKYTR 1050
    KFIANTVEKL DKELSKAEAS SSKSNVPQSE ANIFDSIYIS ANKNNVTDLR 1100
    RNLDGEIQGV QNSFSDVSKV NLRPSKHYKA LIQLNKVHVN LKNYRVDEPD 1150
    ESNSDNSTDV LNRCVVSIYE FEIIDNVPTS TWNKFVTLLK HEPWPHSSPM 1200
    FLLDLEFIRP IDFLQAVELV MQLNVAPLRL HVDQDTLEFL IRFLGFKDKR 1250
    FELIDEYPDI VFIQKFSTNS IKLRLDYKPK KVDYAGLRSG QTSELMNFFT 1300
    LDGSKIILKS VVLYGLNGFD ELNNKLKAIW TPDITKKQLP GVLEGLAPVR 1350
    SFMAIGSGVK TLVTVLMSEY RQEGHLGRSL KKGGNVFLKT TTGDFVKLGV 1400
    KLTSGTQAIL ENTEELFGGV GSNGRVYDAS KFGSADGADS DTAAVLDLDT 1450
    LFEEDQLVGS KYSRIRDHEP TAVVIDMSSP GDHNEPTIVS LYADQPLDLP 1500
    TGLKEAYSSL EKHMHIAYDA VWRAKGQMKD DKRGGPSAAA VYVARAAPVA 1550
    IIRPLIGATE AVSKTLQGIA NQVDKTHNEQ INDKYKSNRT DS 1592
    Length:1,592
    Mass (Da):178,414
    Last modified:October 1, 1996 - v1
    Checksum:i54FDDF56297FFF99
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69381 Genomic DNA. Translation: CAA93356.1.
    Z71518 Genomic DNA. Translation: CAA96147.1.
    BK006947 Genomic DNA. Translation: DAA10317.1.
    PIRiS63208.
    RefSeqiNP_014157.1. NM_001183080.1.

    Genome annotation databases

    EnsemblFungiiYNL242W; YNL242W; YNL242W.
    GeneIDi855479.
    KEGGisce:YNL242W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69381 Genomic DNA. Translation: CAA93356.1 .
    Z71518 Genomic DNA. Translation: CAA96147.1 .
    BK006947 Genomic DNA. Translation: DAA10317.1 .
    PIRi S63208.
    RefSeqi NP_014157.1. NM_001183080.1.

    3D structure databases

    ProteinModelPortali P53855.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35597. 18 interactions.
    DIPi DIP-2620N.
    IntActi P53855. 4 interactions.
    MINTi MINT-426483.
    STRINGi 4932.YNL242W.

    Protein family/group databases

    TCDBi 9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

    Proteomic databases

    MaxQBi P53855.
    PaxDbi P53855.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL242W ; YNL242W ; YNL242W .
    GeneIDi 855479.
    KEGGi sce:YNL242W.

    Organism-specific databases

    CYGDi YNL242w.
    SGDi S000005186. ATG2.

    Phylogenomic databases

    eggNOGi NOG304940.
    GeneTreei ENSGT00620000087966.
    HOGENOMi HOG000034152.
    KOi K17906.
    OMAi MSSWLPQ.
    OrthoDBi EOG7B31W6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33239-MONOMER.

    Miscellaneous databases

    NextBioi 979442.

    Gene expression databases

    Genevestigatori P53855.

    Family and domain databases

    InterProi IPR026849. ATG2.
    IPR026885. ATG2_CAD_motif.
    IPR015412. Autophagy-rel_C.
    [Graphical view ]
    PANTHERi PTHR13190. PTHR13190. 1 hit.
    Pfami PF13329. ATG2_CAD. 1 hit.
    PF09333. ATG_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
      Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
      Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
      Tsukada M., Ohsumi Y.
      FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Disruption of six unknown open reading frames from Saccharomyces cerevisiae reveals two genes involved in vacuolar morphogenesis and one gene required for sporulation."
      Saiz J.E., de Los Angeles Santos M., Vazquez de Aldana C.R., Revuelta J.L.
      Yeast 15:155-164(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "A genomic screen identifies AUT8 as a novel gene essential for autophagy in the yeast Saccharomyces cerevisiae."
      Barth H., Thumm M.
      Gene 274:151-156(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways."
      Wang C.-W., Kim J., Huang W.-P., Abeliovich H., Stromhaug P.E., Dunn W.A. Jr., Klionsky D.J.
      J. Biol. Chem. 276:30442-30451(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Apg2p functions in autophagosome formation on the perivacuolar structure."
      Shintani T., Suzuki K., Kamada Y., Noda T., Ohsumi Y.
      J. Biol. Chem. 276:30452-30460(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-83.
    9. Cited for: NOMENCLATURE.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure."
      Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J.
      Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
      Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
      Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG18.
    13. "The Atg18-Atg2 complex is recruited to autophagic membranes via phosphatidylinositol 3-phosphate and exerts an essential function."
      Obara K., Sekito T., Niimi K., Ohsumi Y.
      J. Biol. Chem. 283:23972-23980(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG18, SUBCELLULAR LOCATION.
    14. "Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy."
      Geng J., Baba M., Nair U., Klionsky D.J.
      J. Cell Biol. 182:129-140(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy."
      He C., Baba M., Cao Y., Klionsky D.J.
      Mol. Biol. Cell 19:5506-5516(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "Atg17 recruits Atg9 to organize the pre-autophagosomal structure."
      Sekito T., Kawamata T., Ichikawa R., Suzuki K., Ohsumi Y.
      Genes Cells 14:525-538(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    19. "Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae."
      van der Vaart A., Griffith J., Reggiori F.
      Mol. Biol. Cell 21:2270-2284(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    20. "Tor directly controls the Atg1 kinase complex to regulate autophagy."
      Kamada Y., Yoshino K., Kondo C., Kawamata T., Oshiro N., Yonezawa K., Ohsumi Y.
      Mol. Cell. Biol. 30:1049-1058(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    21. "Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2."
      Kobayashi T., Suzuki K., Ohsumi Y.
      FEBS Lett. 586:2473-2478(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    22. "Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18."
      Watanabe Y., Kobayashi T., Yamamoto H., Hoshida H., Akada R., Inagaki F., Ohsumi Y., Noda N.N.
      J. Biol. Chem. 287:31681-31690(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG18.
    23. "Atg18 function in autophagy is regulated by specific sites within its beta-propeller."
      Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F.
      J. Cell Sci. 126:593-604(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG18, FUNCTION.
    24. "Autophagy and leucine promote chronological longevity and respiration proficiency during calorie restriction in yeast."
      Aris J.P., Alvers A.L., Ferraiuolo R.A., Fishwick L.K., Hanvivatpong A., Hu D., Kirlew C., Leonard M.T., Losin K.J., Marraffini M., Seo A.Y., Swanberg V., Westcott J.L., Wood M.S., Leeuwenburgh C., Dunn W.A. Jr.
      Exp. Gerontol. 48:1107-1119(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiATG2_YEAST
    AccessioniPrimary (citable) accession number: P53855
    Secondary accession number(s): D6W0V1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 876 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3