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P53855 (ATG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 2
Alternative name(s):
Sporulation-specific protein 72
Gene names
Name:ATG2
Synonyms:APG2, AUT8, SPO72
Ordered Locus Names:YNL242W
ORF Names:N1106
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1592 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for cytoplasm to vacuole transport (Cvt) vesicle and autophagosome completion. Necessary for the localization of ATG18 to the preautophagosomal structure (PAS) and the binding of ATG18 to ATG9. Involved in correct ATG9 trafficking through the preautophagosomal structure and in peroxisome degradation. ATG2 is the most downstream ATG protein in the preautophagosomal structure organization process. Plays a significant role in life span extension. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.11 Ref.12 Ref.13 Ref.14 Ref.17 Ref.20 Ref.21 Ref.23 Ref.24

Subunit structure

Interacts with ATG18. Ref.12 Ref.13 Ref.22 Ref.23

Subcellular location

Preautophagosomal structure membrane; Peripheral membrane protein. Note: ATG1 and ATG9 are required for the proper localization the PAS. Ref.8 Ref.11 Ref.12 Ref.13 Ref.15 Ref.19 Ref.21

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ATG2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15921592Autophagy-related protein 2
PRO_0000215836

Regions

Coiled coil1049 – 107527 Potential

Amino acid modifications

Modified residue2361Phosphoserine Ref.18

Experimental info

Mutagenesis831G → E: Leads to a severely reduced activity of autophagy and a dispersed localization in the cytoplasm. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P53855 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 54FDDF56297FFF99

FASTA1,592178,414
        10         20         30         40         50         60 
MAFWLPQNIQ KRLLLYVLQQ ISLFSNIDLS NLDVSIGSKS HFSFHDVNLS LDDLNIPNVQ 

        70         80         90        100        110        120 
INEGIVDELV LKLTVSGGVE IDGSGLRFIM TPLYSSGSQE LHSDFLVKSI QDLTNSMLQF 

       130        140        150        160        170        180 
SDPLTTYNRY KEDDISSSDS SSDLNSNIEA SKPAANGSYT LQNMRNKALN VALAKLKIAL 

       190        200        210        220        230        240 
KDVTIRFIVN DRDPSDNIVE VHLESIQLIT TDANLRHINI ENITISSIQK QAVPDSPVHP 

       250        260        270        280        290        300 
FNNDDLSQSV YLSKMEATSL YMSAMEEQSN EDPSEPQVTQ EEQENDKCKE SLMEINNLNI 

       310        320        330        340        350        360 
AFKGLSSVND LRMSNIVIDI QDVHLAIHKI VEIKNSTLKN IIDIIVTHLD ANESFSCQDS 

       370        380        390        400        410        420 
QSPSPDKQEP SALSSVDIKC IYLNLGQDIT VILKSFKLEQ KENNSLAFSL GSFYSNSSPL 

       430        440        450        460        470        480 
TISHKTKPLL TGEQTPQSIA LNMGDELDII ISHDGIAHFF KIFQFVSKCM SFYQNKSKGM 

       490        500        510        520        530        540 
MPQIASDTKR TVQLTSKAVK LSLKFPYFLL CFQVSPFIYD SNRELYIELV DVFKKLPSRC 

       550        560        570        580        590        600 
TKILTMSSIT ISNLQSPLQL GSYDDTLKEA LIYSSVHAII KEVIFNEEYS GIVQLVEDIS 

       610        620        630        640        650        660 
AFGKLFTDSK NSECTGKSKS KRGSFLQRSV RVLNSSRFVY KQSLSANFSL KIDSMKLKVS 

       670        680        690        700        710        720 
EIIGPQFGSV EALLSNNFFA ITDDSQIVYF TKNLKVERKT PSLLEPQEIM SVVLNKAVNE 

       730        740        750        760        770        780 
PVLYVHRRAN GKLKVIFNNI RIHYYARWLE ILKKNIGPDN ASSKDEPVSQ KLSKKQPTSG 

       790        800        810        820        830        840 
FPWELKCLDC SLILHPFRLK SVMVIVLDNL TTGGSSFIPQ AKLLSKANTL FLIDDYQNFK 

       850        860        870        880        890        900 
IQKDKNWPSL INFYAGQGFS AIGKIDTLNF LINKSDGALL LDCKIEQVGL SLCADSFQTF 

       910        920        930        940        950        960 
CQLCIDLKYP QTFPDEEKFR TQLKNPIDVF KDIDCDLFNS AFIRENNHQN DYDSVHLVDS 

       970        980        990       1000       1010       1020 
FLDKTHEFNN GARSKLSSQG SYEMDSSSGT ATGGILLPHE SYLDSAQPKE EDTPPIASKE 

      1030       1040       1050       1060       1070       1080 
QERDVDIRGS IDVEKVVIKL FDGYDWKYTR KFIANTVEKL DKELSKAEAS SSKSNVPQSE 

      1090       1100       1110       1120       1130       1140 
ANIFDSIYIS ANKNNVTDLR RNLDGEIQGV QNSFSDVSKV NLRPSKHYKA LIQLNKVHVN 

      1150       1160       1170       1180       1190       1200 
LKNYRVDEPD ESNSDNSTDV LNRCVVSIYE FEIIDNVPTS TWNKFVTLLK HEPWPHSSPM 

      1210       1220       1230       1240       1250       1260 
FLLDLEFIRP IDFLQAVELV MQLNVAPLRL HVDQDTLEFL IRFLGFKDKR FELIDEYPDI 

      1270       1280       1290       1300       1310       1320 
VFIQKFSTNS IKLRLDYKPK KVDYAGLRSG QTSELMNFFT LDGSKIILKS VVLYGLNGFD 

      1330       1340       1350       1360       1370       1380 
ELNNKLKAIW TPDITKKQLP GVLEGLAPVR SFMAIGSGVK TLVTVLMSEY RQEGHLGRSL 

      1390       1400       1410       1420       1430       1440 
KKGGNVFLKT TTGDFVKLGV KLTSGTQAIL ENTEELFGGV GSNGRVYDAS KFGSADGADS 

      1450       1460       1470       1480       1490       1500 
DTAAVLDLDT LFEEDQLVGS KYSRIRDHEP TAVVIDMSSP GDHNEPTIVS LYADQPLDLP 

      1510       1520       1530       1540       1550       1560 
TGLKEAYSSL EKHMHIAYDA VWRAKGQMKD DKRGGPSAAA VYVARAAPVA IIRPLIGATE 

      1570       1580       1590 
AVSKTLQGIA NQVDKTHNEQ INDKYKSNRT DS 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
Tsukada M., Ohsumi Y.
FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Disruption of six unknown open reading frames from Saccharomyces cerevisiae reveals two genes involved in vacuolar morphogenesis and one gene required for sporulation."
Saiz J.E., de Los Angeles Santos M., Vazquez de Aldana C.R., Revuelta J.L.
Yeast 15:155-164(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"A genomic screen identifies AUT8 as a novel gene essential for autophagy in the yeast Saccharomyces cerevisiae."
Barth H., Thumm M.
Gene 274:151-156(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways."
Wang C.-W., Kim J., Huang W.-P., Abeliovich H., Stromhaug P.E., Dunn W.A. Jr., Klionsky D.J.
J. Biol. Chem. 276:30442-30451(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Apg2p functions in autophagosome formation on the perivacuolar structure."
Shintani T., Suzuki K., Kamada Y., Noda T., Ohsumi Y.
J. Biol. Chem. 276:30452-30460(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-83.
[9]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure."
Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J.
Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]"Hierarchy of Atg proteins in pre-autophagosomal structure organization."
Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG18.
[13]"The Atg18-Atg2 complex is recruited to autophagic membranes via phosphatidylinositol 3-phosphate and exerts an essential function."
Obara K., Sekito T., Niimi K., Ohsumi Y.
J. Biol. Chem. 283:23972-23980(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG18, SUBCELLULAR LOCATION.
[14]"Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy."
Geng J., Baba M., Nair U., Klionsky D.J.
J. Cell Biol. 182:129-140(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy."
He C., Baba M., Cao Y., Klionsky D.J.
Mol. Biol. Cell 19:5506-5516(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[16]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"Atg17 recruits Atg9 to organize the pre-autophagosomal structure."
Sekito T., Kawamata T., Ichikawa R., Suzuki K., Ohsumi Y.
Genes Cells 14:525-538(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[18]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[19]"Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae."
van der Vaart A., Griffith J., Reggiori F.
Mol. Biol. Cell 21:2270-2284(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[20]"Tor directly controls the Atg1 kinase complex to regulate autophagy."
Kamada Y., Yoshino K., Kondo C., Kawamata T., Oshiro N., Yonezawa K., Ohsumi Y.
Mol. Cell. Biol. 30:1049-1058(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[21]"Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2."
Kobayashi T., Suzuki K., Ohsumi Y.
FEBS Lett. 586:2473-2478(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[22]"Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18."
Watanabe Y., Kobayashi T., Yamamoto H., Hoshida H., Akada R., Inagaki F., Ohsumi Y., Noda N.N.
J. Biol. Chem. 287:31681-31690(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG18.
[23]"Atg18 function in autophagy is regulated by specific sites within its beta-propeller."
Rieter E., Vinke F., Bakula D., Cebollero E., Ungermann C., Proikas-Cezanne T., Reggiori F.
J. Cell Sci. 126:593-604(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG18, FUNCTION.
[24]"Autophagy and leucine promote chronological longevity and respiration proficiency during calorie restriction in yeast."
Aris J.P., Alvers A.L., Ferraiuolo R.A., Fishwick L.K., Hanvivatpong A., Hu D., Kirlew C., Leonard M.T., Losin K.J., Marraffini M., Seo A.Y., Swanberg V., Westcott J.L., Wood M.S., Leeuwenburgh C., Dunn W.A. Jr.
Exp. Gerontol. 48:1107-1119(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z69381 Genomic DNA. Translation: CAA93356.1.
Z71518 Genomic DNA. Translation: CAA96147.1.
BK006947 Genomic DNA. Translation: DAA10317.1.
PIRS63208.
RefSeqNP_014157.1. NM_001183080.1.

3D structure databases

ProteinModelPortalP53855.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35597. 18 interactions.
DIPDIP-2620N.
IntActP53855. 4 interactions.
MINTMINT-426483.
STRING4932.YNL242W.

Protein family/group databases

TCDB9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Proteomic databases

PaxDbP53855.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL242W; YNL242W; YNL242W.
GeneID855479.
KEGGsce:YNL242W.

Organism-specific databases

CYGDYNL242w.
SGDS000005186. ATG2.

Phylogenomic databases

eggNOGNOG304940.
GeneTreeENSGT00620000087966.
HOGENOMHOG000034152.
KOK17906.
OMASELMNFF.
OrthoDBEOG7B31W6.

Enzyme and pathway databases

BioCycYEAST:G3O-33239-MONOMER.

Gene expression databases

GenevestigatorP53855.

Family and domain databases

InterProIPR026849. ATG2.
IPR026885. ATG2_CAD_motif.
IPR015412. Autophagy-rel_C.
[Graphical view]
PANTHERPTHR13190. PTHR13190. 1 hit.
PfamPF13329. ATG2_CAD. 1 hit.
PF09333. ATG_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979442.

Entry information

Entry nameATG2_YEAST
AccessionPrimary (citable) accession number: P53855
Secondary accession number(s): D6W0V1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families