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Protein

Pre-mRNA-splicing factor CWC25

Gene

CWC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing.1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-33242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC25
Alternative name(s):
Complexed with CEF1 protein 25
Gene namesi
Name:CWC25
Ordered Locus Names:YNL245C
ORF Names:N0901
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL245C.
SGDiS000005189. CWC25.

Subcellular locationi

GO - Cellular componenti

  • U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000795961 – 179Pre-mRNA-splicing factor CWC25Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53854.
PRIDEiP53854.
TopDownProteomicsiP53854.

PTM databases

iPTMnetiP53854.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CLF1, PRP8, PRP19 and SNU114.2 Publications

Protein-protein interaction databases

BioGridi35594. 39 interactors.
DIPiDIP-2781N.
IntActiP53854. 18 interactors.
MINTiMINT-1603940.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMKelectron microscopy3.40G1-179[»]
5LJ3electron microscopy3.80F1-179[»]
5LJ5electron microscopy3.80F1-179[»]
ProteinModelPortaliP53854.
SMRiP53854.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 57Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the CWC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000112092.
InParanoidiP53854.
OMAiQPTSHEY.
OrthoDBiEOG092C579Z.

Family and domain databases

InterProiIPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view]
PfamiPF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view]
SMARTiSM01083. Cir_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGDLNLLK SWNPKLMKNR KKVWETEQDL ITEQQKLNTR LKEIEKEREL
60 70 80 90 100
NELLNESSKD KPETLKNDLA LKKSGLEWMY QDAKLSDEKE DYLLGKKKLD
110 120 130 140 150
SSILNQPATP PVRAATTISA SGAATSISSQ KKKSKLLKDD PMSKFKVTKQ
160 170
QRRTPDSTKK RAMSQRGKPL SKPAPDLDY
Length:179
Mass (Da):20,374
Last modified:October 1, 1996 - v1
Checksum:iC6146E12248E48E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96722 Genomic DNA. Translation: CAA65498.1.
Z71522 Genomic DNA. Translation: CAA96153.1.
AY693108 Genomic DNA. Translation: AAT93127.1.
BK006947 Genomic DNA. Translation: DAA10314.1.
PIRiS63213.
RefSeqiNP_014154.1. NM_001183083.1.

Genome annotation databases

EnsemblFungiiYNL245C; YNL245C; YNL245C.
GeneIDi855476.
KEGGisce:YNL245C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96722 Genomic DNA. Translation: CAA65498.1.
Z71522 Genomic DNA. Translation: CAA96153.1.
AY693108 Genomic DNA. Translation: AAT93127.1.
BK006947 Genomic DNA. Translation: DAA10314.1.
PIRiS63213.
RefSeqiNP_014154.1. NM_001183083.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMKelectron microscopy3.40G1-179[»]
5LJ3electron microscopy3.80F1-179[»]
5LJ5electron microscopy3.80F1-179[»]
ProteinModelPortaliP53854.
SMRiP53854.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35594. 39 interactors.
DIPiDIP-2781N.
IntActiP53854. 18 interactors.
MINTiMINT-1603940.

PTM databases

iPTMnetiP53854.

Proteomic databases

MaxQBiP53854.
PRIDEiP53854.
TopDownProteomicsiP53854.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL245C; YNL245C; YNL245C.
GeneIDi855476.
KEGGisce:YNL245C.

Organism-specific databases

EuPathDBiFungiDB:YNL245C.
SGDiS000005189. CWC25.

Phylogenomic databases

HOGENOMiHOG000112092.
InParanoidiP53854.
OMAiQPTSHEY.
OrthoDBiEOG092C579Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-33242-MONOMER.

Miscellaneous databases

PROiP53854.

Family and domain databases

InterProiIPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view]
PfamiPF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view]
SMARTiSM01083. Cir_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCWC25_YEAST
AccessioniPrimary (citable) accession number: P53854
Secondary accession number(s): D6W0U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.