P53854 (CWC25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor CWC25

Alternative name(s):
Complexed with CEF1 protein 25

Gene names

Name:	CWC25
Ordered Locus Names:	YNL245C
ORF Names:	N0901

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. Ref.6
Subunit structure	Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CLF1, PRP8, PRP19 and SNU114. Ref.5 Ref.6
Subcellular location	Nucleus Ref.6 Ref.7.
Miscellaneous	Present with 861 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CWC25 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from direct assay PubMed 19935684. Source: SGD
Cellular_component	U2-type spliceosomal complex Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Pre-mRNA-splicing factor CWC25		PRO_0000079596
Regions
	Coiled coil	25 – 57	33	Potential

Sequences

	Sequence		Length	Mass (Da)
	P53854 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C6146E12248E48E8	FASTA	179	20,374
	10 20 30 40 50 60 MGSGDLNLLK SWNPKLMKNR KKVWETEQDL ITEQQKLNTR LKEIEKEREL NELLNESSKD 70 80 90 100 110 120 KPETLKNDLA LKKSGLEWMY QDAKLSDEKE DYLLGKKKLD SSILNQPATP PVRAATTISA 130 140 150 160 170 SGAATSISSQ KKKSKLLKDD PMSKFKVTKQ QRRTPDSTKK RAMSQRGKPL SKPAPDLDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae." Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H. Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[6]	"Assigning function to yeast proteins by integration of technologies." Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M., Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E., Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N. Mol. Cell 12:1353-1365(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, INTERACTION WITH CLF1; PRP8; PRP19 AND SNU114, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96722 Genomic DNA. Translation: CAA65498.1. Z71522 Genomic DNA. Translation: CAA96153.1. AY693108 Genomic DNA. Translation: AAT93127.1. BK006947 Genomic DNA. Translation: DAA10314.1.
PIR	S63213.
RefSeq	NP_014154.1. NM_001183083.1.
3D structure databases
ProteinModelPortal	P53854.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2781N.
IntAct	P53854. 17 interactions.
STRING	4932.YNL245C.
Proteomic databases
PaxDb	P53854.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL245C; YNL245C; YNL245C.
GeneID	855476.
KEGG	sce:YNL245C.
Organism-specific databases
CYGD	YNL245c.
SGD	S000005189. CWC25.
Phylogenomic databases
eggNOG	NOG265524.
GeneTree	ENSGT00440000039055.
HOGENOM	HOG000112092.
OMA	PTSHEYP.
OrthoDB	EOG4V46JB.
Gene expression databases
Genevestigator	P53854.
GermOnline	YNL245C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019339. CIR_N_dom. IPR022209. CWC25. [Graphical view]
Pfam	PF10197. Cir_N. 1 hit. PF12542. CWC25. 1 hit. [Graphical view]
SMART	SM01083. Cir_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979433.

Entry information

Entry name

CWC25_YEAST

Accession

Primary (citable) accession number: P53854
Secondary accession number(s): D6W0U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program