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P53854

- CWC25_YEAST

UniProt

P53854 - CWC25_YEAST

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Protein

Pre-mRNA-splicing factor CWC25

Gene

CWC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing.1 Publication

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-33242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC25
Alternative name(s):
Complexed with CEF1 protein 25
Gene namesi
Name:CWC25
Ordered Locus Names:YNL245C
ORF Names:N0901
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL245c.
SGDiS000005189. CWC25.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Pre-mRNA-splicing factor CWC25PRO_0000079596Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53854.
PaxDbiP53854.

Expressioni

Gene expression databases

GenevestigatoriP53854.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CLF1, PRP8, PRP19 and SNU114.2 Publications

Protein-protein interaction databases

BioGridi35594. 39 interactions.
DIPiDIP-2781N.
IntActiP53854. 17 interactions.
MINTiMINT-1603940.
STRINGi4932.YNL245C.

Structurei

3D structure databases

ProteinModelPortaliP53854.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 5733Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the CWC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG265524.
HOGENOMiHOG000112092.
InParanoidiP53854.
OMAiRAPHACA.
OrthoDBiEOG7KH9Z9.

Family and domain databases

InterProiIPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view]
PfamiPF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view]
SMARTiSM01083. Cir_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53854-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSGDLNLLK SWNPKLMKNR KKVWETEQDL ITEQQKLNTR LKEIEKEREL
60 70 80 90 100
NELLNESSKD KPETLKNDLA LKKSGLEWMY QDAKLSDEKE DYLLGKKKLD
110 120 130 140 150
SSILNQPATP PVRAATTISA SGAATSISSQ KKKSKLLKDD PMSKFKVTKQ
160 170
QRRTPDSTKK RAMSQRGKPL SKPAPDLDY
Length:179
Mass (Da):20,374
Last modified:October 1, 1996 - v1
Checksum:iC6146E12248E48E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96722 Genomic DNA. Translation: CAA65498.1.
Z71522 Genomic DNA. Translation: CAA96153.1.
AY693108 Genomic DNA. Translation: AAT93127.1.
BK006947 Genomic DNA. Translation: DAA10314.1.
PIRiS63213.
RefSeqiNP_014154.1. NM_001183083.1.

Genome annotation databases

EnsemblFungiiYNL245C; YNL245C; YNL245C.
GeneIDi855476.
KEGGisce:YNL245C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96722 Genomic DNA. Translation: CAA65498.1 .
Z71522 Genomic DNA. Translation: CAA96153.1 .
AY693108 Genomic DNA. Translation: AAT93127.1 .
BK006947 Genomic DNA. Translation: DAA10314.1 .
PIRi S63213.
RefSeqi NP_014154.1. NM_001183083.1.

3D structure databases

ProteinModelPortali P53854.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35594. 39 interactions.
DIPi DIP-2781N.
IntActi P53854. 17 interactions.
MINTi MINT-1603940.
STRINGi 4932.YNL245C.

Proteomic databases

MaxQBi P53854.
PaxDbi P53854.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL245C ; YNL245C ; YNL245C .
GeneIDi 855476.
KEGGi sce:YNL245C.

Organism-specific databases

CYGDi YNL245c.
SGDi S000005189. CWC25.

Phylogenomic databases

eggNOGi NOG265524.
HOGENOMi HOG000112092.
InParanoidi P53854.
OMAi RAPHACA.
OrthoDBi EOG7KH9Z9.

Enzyme and pathway databases

BioCyci YEAST:G3O-33242-MONOMER.

Miscellaneous databases

NextBioi 979433.

Gene expression databases

Genevestigatori P53854.

Family and domain databases

InterProi IPR019339. CIR_N_dom.
IPR022209. CWC25.
[Graphical view ]
Pfami PF10197. Cir_N. 1 hit.
PF12542. CWC25. 1 hit.
[Graphical view ]
SMARTi SM01083. Cir_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H.
    Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CLF1; PRP8; PRP19 AND SNU114, SUBCELLULAR LOCATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCWC25_YEAST
AccessioniPrimary (citable) accession number: P53854
Secondary accession number(s): D6W0U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 861 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3