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P53854

- CWC25_YEAST

UniProt

P53854 - CWC25_YEAST

Protein

Pre-mRNA-splicing factor CWC25

Gene

CWC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing.1 Publication

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33242-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor CWC25
    Alternative name(s):
    Complexed with CEF1 protein 25
    Gene namesi
    Name:CWC25
    Ordered Locus Names:YNL245C
    ORF Names:N0901
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL245c.
    SGDiS000005189. CWC25.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. U2-type spliceosomal complex Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Pre-mRNA-splicing factor CWC25PRO_0000079596Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei129 – 1291Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53854.
    PaxDbiP53854.

    Expressioni

    Gene expression databases

    GenevestigatoriP53854.

    Interactioni

    Subunit structurei

    Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CLF1, PRP8, PRP19 and SNU114.2 Publications

    Protein-protein interaction databases

    BioGridi35594. 39 interactions.
    DIPiDIP-2781N.
    IntActiP53854. 17 interactions.
    MINTiMINT-1603940.
    STRINGi4932.YNL245C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53854.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili25 – 5733Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CWC25 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG265524.
    HOGENOMiHOG000112092.
    OMAiRAPHACA.
    OrthoDBiEOG7KH9Z9.

    Family and domain databases

    InterProiIPR019339. CIR_N_dom.
    IPR022209. CWC25.
    [Graphical view]
    PfamiPF10197. Cir_N. 1 hit.
    PF12542. CWC25. 1 hit.
    [Graphical view]
    SMARTiSM01083. Cir_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53854-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSGDLNLLK SWNPKLMKNR KKVWETEQDL ITEQQKLNTR LKEIEKEREL    50
    NELLNESSKD KPETLKNDLA LKKSGLEWMY QDAKLSDEKE DYLLGKKKLD 100
    SSILNQPATP PVRAATTISA SGAATSISSQ KKKSKLLKDD PMSKFKVTKQ 150
    QRRTPDSTKK RAMSQRGKPL SKPAPDLDY 179
    Length:179
    Mass (Da):20,374
    Last modified:October 1, 1996 - v1
    Checksum:iC6146E12248E48E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96722 Genomic DNA. Translation: CAA65498.1.
    Z71522 Genomic DNA. Translation: CAA96153.1.
    AY693108 Genomic DNA. Translation: AAT93127.1.
    BK006947 Genomic DNA. Translation: DAA10314.1.
    PIRiS63213.
    RefSeqiNP_014154.1. NM_001183083.1.

    Genome annotation databases

    EnsemblFungiiYNL245C; YNL245C; YNL245C.
    GeneIDi855476.
    KEGGisce:YNL245C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96722 Genomic DNA. Translation: CAA65498.1 .
    Z71522 Genomic DNA. Translation: CAA96153.1 .
    AY693108 Genomic DNA. Translation: AAT93127.1 .
    BK006947 Genomic DNA. Translation: DAA10314.1 .
    PIRi S63213.
    RefSeqi NP_014154.1. NM_001183083.1.

    3D structure databases

    ProteinModelPortali P53854.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35594. 39 interactions.
    DIPi DIP-2781N.
    IntActi P53854. 17 interactions.
    MINTi MINT-1603940.
    STRINGi 4932.YNL245C.

    Proteomic databases

    MaxQBi P53854.
    PaxDbi P53854.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL245C ; YNL245C ; YNL245C .
    GeneIDi 855476.
    KEGGi sce:YNL245C.

    Organism-specific databases

    CYGDi YNL245c.
    SGDi S000005189. CWC25.

    Phylogenomic databases

    eggNOGi NOG265524.
    HOGENOMi HOG000112092.
    OMAi RAPHACA.
    OrthoDBi EOG7KH9Z9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33242-MONOMER.

    Miscellaneous databases

    NextBioi 979433.

    Gene expression databases

    Genevestigatori P53854.

    Family and domain databases

    InterProi IPR019339. CIR_N_dom.
    IPR022209. CWC25.
    [Graphical view ]
    Pfami PF10197. Cir_N. 1 hit.
    PF12542. CWC25. 1 hit.
    [Graphical view ]
    SMARTi SM01083. Cir_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H.
      Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CLF1; PRP8; PRP19 AND SNU114, SUBCELLULAR LOCATION.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCWC25_YEAST
    AccessioniPrimary (citable) accession number: P53854
    Secondary accession number(s): D6W0U8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 861 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3