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Protein

Protein transport protein DSL1

Gene

DSL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.3 Publications

GO - Biological processi

  • ER-dependent peroxisome organization Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33254-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein DSL1
Alternative name(s):
Dependent on SLY1-20 protein 1
Gene namesi
Name:DSL1
Ordered Locus Names:YNL258C
ORF Names:N0842
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL258C.
SGDiS000005202. DSL1.

Subcellular locationi

GO - Cellular componenti

  • Dsl1p complex Source: SGD
  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi413 – 4131W → A: Viable and reduced interaction with RET2, strong Golgi-ER retrograde transport defect. Loss of interaction with RET2; when associated with A-455. Lethal and loss of interactions with RET1 and RET2; when associated with A-425. 1 Publication
Mutagenesisi425 – 4251W → A: Loss of interaction with RET1; when associated with A-413. 1 Publication
Mutagenesisi455 – 4551W → A: Viable and no effect. Lethal and loss of interaction with RET2 and reduced interaction with RET1; when associated with A-413. 1 Publication
Mutagenesisi725 – 75430Missing in dsl1-22; strong Golgi-ER retrograde transport defect. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Protein transport protein DSL1PRO_0000203383Add
BLAST

Proteomic databases

MaxQBiP53847.

PTM databases

iPTMnetiP53847.

Interactioni

Subunit structurei

Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Binds the coatomer complex through direct interaction with RET2/COPD and RET1/COPA. Binds TIP20 and SEC39/DSL3.

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC39Q127458EBI-29249,EBI-31898
TIP20P338917EBI-29249,EBI-19396

Protein-protein interaction databases

BioGridi35581. 69 interactions.
DIPiDIP-4274N.
IntActiP53847. 9 interactions.
MINTiMINT-473831.

Structurei

Secondary structure

1
754
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 7329Combined sources
Helixi77 – 9317Combined sources
Helixi95 – 984Combined sources
Helixi102 – 12928Combined sources
Beta strandi132 – 1343Combined sources
Beta strandi136 – 1416Combined sources
Beta strandi143 – 1475Combined sources
Turni148 – 1514Combined sources
Beta strandi152 – 1554Combined sources
Helixi156 – 16712Combined sources
Helixi169 – 1713Combined sources
Helixi178 – 1814Combined sources
Helixi186 – 20318Combined sources
Helixi207 – 21610Combined sources
Beta strandi222 – 2276Combined sources
Turni228 – 2314Combined sources
Beta strandi232 – 2376Combined sources
Helixi246 – 26217Combined sources
Helixi266 – 29025Combined sources
Helixi292 – 2954Combined sources
Beta strandi297 – 3004Combined sources
Helixi302 – 32120Combined sources
Helixi331 – 3366Combined sources
Helixi340 – 35415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ETUX-ray2.40A1-361[»]
3ETVX-ray1.94A37-339[»]
5FJWX-ray2.80L/M/N/O/P/Q/R/S411-419[»]
5FJZX-ray1.90P/Q/R/S411-419[»]
ProteinModelPortaliP53847.
SMRiP53847. Positions 37-355, 517-751.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53847.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 200200Interaction with TIP20Add
BLAST
Regioni406 – 45954Interaction with RET2Add
BLAST
Regioni406 – 44035Interaction with RET1Add
BLAST

Phylogenomic databases

HOGENOMiHOG000112285.
InParanoidiP53847.
OMAiLENCYYS.
OrthoDBiEOG092C0TGT.

Family and domain databases

InterProiIPR021876. Dsl1_C.
IPR021875. Dsl1_N_dom.
[Graphical view]
PfamiPF11989. Dsl1_C. 1 hit.
PF11988. Dsl1_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLFPNKGE IIRELLKDPL ILKNDSKRSN GSELELDSSD LLQREAILAN
60 70 80 90 100
ELNILDNLKT FLNLIKEVKT NLNILELENC YYSLQSLRKK MRNNAAYLKQ
110 120 130 140 150
SFNFQQSIST YVDTLHLELV STLYKILTNG FWKITENSIQ FTPTVEWGKD
160 170 180 190 200
KVHIEYDTFM DFVAQQYFPK GSLDNQAWFI LDMTSADSQE QVRAKLNTIM
210 220 230 240 250
KEYMNLSRIV SMIKNSIFIS GKEISYENEK NILVFSKSSS HGQHCVSTVL
260 270 280 290 300
TSFEAVCDFM LDGLAFRDRK TLSYELGPLF NTEFTKFVKN NASIILESLD
310 320 330 340 350
SPLKNLVSVI NNKLTRLVAK SEVTNWTHSG KEIQDLLMNK QLYYNLLLDK
360 370 380 390 400
VLESHISEIR SIFEDPKKSW QNLEVVELTT SNTNTMSEKI GKNDSDVQNE
410 420 430 440 450
KELHNAVSKD DDWNWEVEDD DADAWGDEID VNIDDEEEKT NQEKEKEPEE
460 470 480 490 500
EENAWDEAWA IDENIDDASL ENGKEHLKAH DVGSLDKDHI EVTQLPKLFL
510 520 530 540 550
AISQNFKSSF ADSHVDEQYF AYKYNLLQTS YMAMCTANFS HNWCQLYVDM
560 570 580 590 600
RYLIERDEKL YRIKELTRNL LETKLNMKYR IVCQLIRHQL TEFRENERNP
610 620 630 640 650
SWDATIEKLL PYILKEIVRP LQKIRGEEGS RYLLSFLNFL YNDCVTKEIL
660 670 680 690 700
KWQIISEVNS ENLGELVSLL VNNTDIQLLA KEPSYKKMRE KFATMGKFLP
710 720 730 740 750
LHLKEIMEMF YNGDFYLFAT DELIQWIELL FADTPLRRNA IDDIYEIRGT

ALDD
Length:754
Mass (Da):88,072
Last modified:October 1, 1996 - v1
Checksum:i51EDB17F2C55A917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96722 Genomic DNA. Translation: CAA65486.1.
Z71534 Genomic DNA. Translation: CAA96165.1.
BK006947 Genomic DNA. Translation: DAA10301.1.
PIRiS63231.
RefSeqiNP_014141.1. NM_001183096.1.

Genome annotation databases

EnsemblFungiiYNL258C; YNL258C; YNL258C.
GeneIDi855463.
KEGGisce:YNL258C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96722 Genomic DNA. Translation: CAA65486.1.
Z71534 Genomic DNA. Translation: CAA96165.1.
BK006947 Genomic DNA. Translation: DAA10301.1.
PIRiS63231.
RefSeqiNP_014141.1. NM_001183096.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ETUX-ray2.40A1-361[»]
3ETVX-ray1.94A37-339[»]
5FJWX-ray2.80L/M/N/O/P/Q/R/S411-419[»]
5FJZX-ray1.90P/Q/R/S411-419[»]
ProteinModelPortaliP53847.
SMRiP53847. Positions 37-355, 517-751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35581. 69 interactions.
DIPiDIP-4274N.
IntActiP53847. 9 interactions.
MINTiMINT-473831.

PTM databases

iPTMnetiP53847.

Proteomic databases

MaxQBiP53847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL258C; YNL258C; YNL258C.
GeneIDi855463.
KEGGisce:YNL258C.

Organism-specific databases

EuPathDBiFungiDB:YNL258C.
SGDiS000005202. DSL1.

Phylogenomic databases

HOGENOMiHOG000112285.
InParanoidiP53847.
OMAiLENCYYS.
OrthoDBiEOG092C0TGT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33254-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP53847.
PROiP53847.

Family and domain databases

InterProiIPR021876. Dsl1_C.
IPR021875. Dsl1_N_dom.
[Graphical view]
PfamiPF11989. Dsl1_C. 1 hit.
PF11988. Dsl1_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDSL1_YEAST
AccessioniPrimary (citable) accession number: P53847
Secondary accession number(s): D6W0T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.