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P53845 (YIF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein YIF1
Alternative name(s):
YIP1-interacting factor 1
Gene names
Name:YIF1
Ordered Locus Names:YNL263C
ORF Names:N0820
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for fusion of ER-derived vesicles with the Golgi during ER-to-Golgi protein transport. May be involved in proper membrane localization of Rab GTPases. Ref.8

Subunit structure

Component of the YIP1-YIF1 complex, composed of at least YIF1, YIP1 and YOS1. The complex interacts with the ER to Golgi SNAREs BOS1 and SEC22. Interacts with the YIP1 family members YIP4 and YIP5, and with the Rab GTPases SEC4, YPT1, YPT6, YPT7, YPT10, YPT11, YPT31, YPT32 and YPT52. Interacts with BTN2 and SNX3. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle › COPII-coated vesicle. Note: Also found in ER-derived COPII-coated vesicles. Ref.5 Ref.10

Sequence similarities

Belongs to the YIF1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Protein transport protein YIF1
PRO_0000203381

Regions

Topological domain1 – 153153Cytoplasmic Potential
Transmembrane154 – 17421Helical; Potential
Topological domain175 – 18814Lumenal Potential
Transmembrane189 – 20921Helical; Potential
Topological domain210 – 2178Cytoplasmic Potential
Transmembrane218 – 23821Helical; Potential
Topological domain239 – 2435Lumenal Potential
Transmembrane244 – 26421Helical; Potential
Topological domain265 – 29329Cytoplasmic Potential
Transmembrane294 – 31320Helical; Potential
Topological domain3141Lumenal Potential

Sequences

Sequence LengthMass (Da)Tools
P53845 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3D1550C65A7D0A92

FASTA31435,498
        10         20         30         40         50         60 
MSYNPYAYAT SEQNGVNDRF SHTPQQQRPM QIPRNTPVNG QGNANMNANV NGSGGGFPFQ 

        70         80         90        100        110        120 
DPRGSMAFQL GQSAFSNFIG QDNFNQFQET VNKATANAAG SQQISTYFQV STRYVINKLK 

       130        140        150        160        170        180 
LILVPFLNGT KNWQRIMDSG NFLPPRDDVN SPDMYMPIMG LVTYILIWNT QQGLKGSFNP 

       190        200        210        220        230        240 
EDLYYKLSST LAFVCLDLLI LKLGLYLLID SKIPSFSLVE LLCYVGYKFV PLILAQLLTN 

       250        260        270        280        290        300 
VTMPFNLNIL IKFYLFIAFG VFLLRSVKFN LLSRSGAEDD DIHVSISKST VKKCNYFLFV 

       310 
YGFIWQNVLM WLMG

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes."
Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.
Yeast 12:505-514(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting."
Matern H.T., Yang X., Andrulis E., Sternglanz R., Trepte H.-H., Gallwitz D.
EMBO J. 19:4485-4492(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP1, TOPOLOGY.
[5]"Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex."
Otte S., Belden W.J., Heidtman M., Liu J., Jensen O.N., Barlowe C.
J. Cell Biol. 152:503-518(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[6]"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
Calero M., Winand N.J., Collins R.N.
FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SEC4; YIP4; YIP5; YPT1; YPT6; YPT7; YPT10; YPT11; YPT31; YPT32 AND YPT52.
[7]"The yeast model for Batten disease: a role for Btn2p in the trafficking of the Golgi-associated vesicular targeting protein, Yif1p."
Chattopadhyay S., Roberts P.M., Pearce D.A.
Biochem. Biophys. Res. Commun. 302:534-538(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BTN2.
[8]"The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles."
Barrowman J., Wang W., Zhang Y., Ferro-Novick S.
J. Biol. Chem. 278:19878-19884(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BOS1; SEC22 AND YPT1.
[9]"The phox homology (PX) domain protein interaction network in yeast."
Vollert C.S., Uetz P.
Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNX3.
[10]"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
Inadome H., Noda Y., Adachi H., Yoda K.
Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[11]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X92494 Genomic DNA. Translation: CAA63234.1.
Z71539 Genomic DNA. Translation: CAA96170.1.
BK006947 Genomic DNA. Translation: DAA10296.1.
PIRS60918.
RefSeqNP_014136.1. NM_001183101.1.

3D structure databases

ProteinModelPortalP53845.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1729N.
IntActP53845. 29 interactions.
MINTMINT-388912.
STRING4932.YNL263C.

Proteomic databases

PaxDbP53845.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL263C; YNL263C; YNL263C.
GeneID855458.
KEGGsce:YNL263C.

Organism-specific databases

CYGDYNL263c.
SGDS000005207. YIF1.

Phylogenomic databases

eggNOGCOG5197.
GeneTreeENSGT00390000009423.
HOGENOMHOG000201672.
OMAANMNANV.
OrthoDBEOG4RFQ2S.

Gene expression databases

GenevestigatorP53845.
GermOnlineYNL263C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR005578. Hrf1.
[Graphical view]
PANTHERPTHR14083. PTHR14083. 1 hit.
PfamPF03878. YIF1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979379.

Entry information

Entry nameYIF1_YEAST
AccessionPrimary (citable) accession number: P53845
Secondary accession number(s): D6W0T0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents