Reviewed, UniProtKB/Swiss-Prot P53843 (IST1_YEAST)

Names and origin

Protein names

Recommended name:
    Vacuolar protein sorting-associated protein IST1
Alternative name(s):
    Increased sodium tolerance protein 1

Gene names

Name:	IST1
Ordered Locus Names:	YNL265C
ORF Names:	N0809

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in a late step in sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins are targeted into the vacuole after fusion of the endosome with the vacuole. Regulates the recruitment of VPS4 to the ESCRT-III complex, probably in conjunction with DID2, and VPS4 catalyzes the disassembly of the ESCRT-III complex. Ref.6 Ref.7
Subunit structure	Interacts with DID2. Interacts with VPS4 (via MIT domain); the interaction prevents VPS4 oligomerization, competes with the binding of VTA1 to VSP4 and diminishes the ATPase activity of VSP4. Ref.6 Ref.7
Subcellular location	Cytoplasm. Endosome. Note: The endosomal location appears to be dependent on DID2. Ref.6 Ref.7
Miscellaneous	Present with 4760 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the IST1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	late endosome to vacuole transport via multivesicular body sorting pathway Ref.7 Inferred from genetic interaction. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endosome Ref.7 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-28245,EBI-28245
	P38800	1	EBI-28245,EBI-24597
DID2	P69771	1	EBI-28245,EBI-2053489
VTA1	Q06263	1	EBI-28245,EBI-37098

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Vacuolar protein sorting-associated protein IST1		PRO_0000076227
Regions
	Region	251 – 298	48	Interaction with VPS4
	Motif	286 – 296	11	MIT-interacting motif
	Compositional bias	190 – 196	7	Poly-Ser
Amino acid modifications
	Modified residue	244	1	Phosphoserine Ref.4 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53843-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F16A94BFC7716A1A	FASTA	298	34,496
	10 20 30 40 50 60 MAPSMIPFTI KLKTCLKMCI QRLRYAQEKQ QAIAKQSRRQ VAQLLLTNKE QKAHYRVETL 70 80 90 100 110 120 IHDDIHIELL EILELYCELL LARVQVINDI STEEQLVKEH MDDGINEAIR SLIYAILFVD 130 140 150 160 170 180 EVKELSQLKD LMAWKINVEF VNGVIADHID VPEKIIKKCS PSVPKEELVD LYLKEIAKTY 190 200 210 220 230 240 DVPYSKLENS LSSSSSNISS DFSDPSGDIE DNDEEKPILA LDNDDNDNAD AKHPITVKKP 250 260 270 280 290 RQNSENIKNE LKIPKDIKKE VIEKKQSEKK TTKRKTKKEQ ENDELDELKK RFDALRRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes." Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H. Yeast 12:505-514(1996) [PubMed: 8740425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, MASS SPECTROMETRY.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, MASS SPECTROMETRY.
[6]	"Ist1 regulates Vps4 localization and assembly." Dimaano C., Jones C.B., Hanono A., Curtiss M., Babst M. Mol. Biol. Cell 19:465-474(2008) [PubMed: 18032582] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS4, SUBCELLULAR LOCATION.
[7]	"Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting." Rue S.M., Mattei S., Saksena S., Emr S.D. Mol. Biol. Cell 19:475-484(2008) [PubMed: 18032584] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DID2 AND VPS4.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X92494 Genomic DNA. No translation available.
Z71542 Genomic DNA. Translation: CAA96172.1.

PIR

S63238.

RefSeq

NP_014134.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GGY	X-ray	1.70	A/B	1-193	[»]
3GGZ	X-ray	3.80	A/B/C/D	1-193	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:6713N.

IntAct

P53843. 22 interactions.

STRING

P53843.

Genome annotation databases

Ensembl

YNL265C; YNL265C; YNL265C; Saccharomyces cerevisiae. [Genome view]

GeneID

855456.

KEGG

sce:YNL265C.

NMPDR

fig|4932.3.peg.5199.

Organism-specific databases

CYGD

YNL265c.

SGD

S000005209. IST1.

Phylogenomic databases

HOGENOM

P53843.

OMA

DIHIELL

OrthoDB

EOG9FJ9RW

Gene expression databases

ArrayExpress

P53843.

Genevestigator

P53843.

GermOnline

YNL265C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005061. DUF292_euk.
[Graphical view]

Pfam

PF03398. DUF292. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

979373.

Entry information

Entry name

IST1_YEAST

Accession

Primary (citable) accession number: P53843

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)