P53843 (IST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein IST1

Alternative name(s):
Increased sodium tolerance protein 1

Gene names

Name:	IST1
Ordered Locus Names:	YNL265C
ORF Names:	N0809

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a late step in sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins are targeted into the vacuole after fusion of the endosome with the vacuole. Regulates the recruitment of VPS4 to the ESCRT-III complex, probably in conjunction with DID2, and VPS4 catalyzes the disassembly of the ESCRT-III complex. Ref.7 Ref.8
Subunit structure	Interacts with DID2. Interacts with VPS4 (via MIT domain); the interaction prevents VPS4 oligomerization, competes with the binding of VTA1 to VSP4 and diminishes the ATPase activity of VSP4. Ref.7 Ref.8
Subcellular location	Cytoplasm. Endosome. Note: The endosomal location appears to be dependent on DID2. Ref.7 Ref.8
Miscellaneous	Present with 4760 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the IST1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endosome Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 298

298

Vacuolar protein sorting-associated protein IST1

PRO_0000076227

Regions

Region

251 – 298

48

Interaction with VPS4

Motif

286 – 296

11

MIT-interacting motif

Compositional bias

190 – 196

7

Poly-Ser

Amino acid modifications

Modified residue

244

1

Phosphoserine Ref.5 Ref.6

Secondary structure

1

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298

Helix Strand Turn

Details...

Helix

8 – 46

39

Helix

50 – 82

33

Helix

84 – 87

4

Helix

93 – 99

7

Helix

106 – 115

10

Helix

116 – 118

3

Helix

125 – 135

11

Helix

138 – 141

4

Turn

145 – 147

3

Helix

153 – 159

7

Helix

166 – 179

14

Helix

184 – 188

5

Sequences

	Sequence		Length	Mass (Da)
	P53843 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F16A94BFC7716A1A	FASTA	298	34,496
	10 20 30 40 50 60 MAPSMIPFTI KLKTCLKMCI QRLRYAQEKQ QAIAKQSRRQ VAQLLLTNKE QKAHYRVETL 70 80 90 100 110 120 IHDDIHIELL EILELYCELL LARVQVINDI STEEQLVKEH MDDGINEAIR SLIYAILFVD 130 140 150 160 170 180 EVKELSQLKD LMAWKINVEF VNGVIADHID VPEKIIKKCS PSVPKEELVD LYLKEIAKTY 190 200 210 220 230 240 DVPYSKLENS LSSSSSNISS DFSDPSGDIE DNDEEKPILA LDNDDNDNAD AKHPITVKKP 250 260 270 280 290 RQNSENIKNE LKIPKDIKKE VIEKKQSEKK TTKRKTKKEQ ENDELDELKK RFDALRRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes." Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H. Yeast 12:505-514(1996) [PubMed: 8740425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244, MASS SPECTROMETRY.
[7]	"Ist1 regulates Vps4 localization and assembly." Dimaano C., Jones C.B., Hanono A., Curtiss M., Babst M. Mol. Biol. Cell 19:465-474(2008) [PubMed: 18032582] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS4, SUBCELLULAR LOCATION.
[8]	"Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting." Rue S.M., Mattei S., Saksena S., Emr S.D. Mol. Biol. Cell 19:475-484(2008) [PubMed: 18032584] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DID2 AND VPS4.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X92494 Genomic DNA. No translation available.
Z71542 Genomic DNA. Translation: CAA96172.1.
BK006947 Genomic DNA. Translation: DAA10294.1.

PIR

S63238.

RefSeq

NP_014134.1. NM_001183103.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GGY	X-ray	1.70	A/B	1-193	[»]
3GGZ	X-ray	3.80	A/B/C/D	1-193	[»]

ProteinModelPortal

P53843.

SMR

P53843. Positions 5-192.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6713N.

IntAct

P53843. 27 interactions.

MINT

MINT-626376.

STRING

P53843.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNL265C; YNL265C; YNL265C.

GeneID

855456.

KEGG

sce:YNL265C.

NMPDR

fig|4932.3.peg.5199.

Organism-specific databases

CYGD

YNL265c.

SGD

S000005209. IST1.

Phylogenomic databases

eggNOG

fuNOG10296.

GeneTree

EFGT00050000005312.

HOGENOM

HBG739022.

OMA

CIQRLRY.

OrthoDB

EOG4C8C2V.

Gene expression databases

ArrayExpress

P53843.

Genevestigator

P53843.

GermOnline

YNL265C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005061. DUF292_euk.
[Graphical view]

Pfam

PF03398. Ist1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

979373.

Entry information

Entry name

IST1_YEAST

Accession

Primary (citable) accession number: P53843
Secondary accession number(s): D6W0S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program