Reviewed, UniProtKB/Swiss-Prot P53841 (BSC4_YEAST)

Names and origin

Protein names

Recommended name:
    Bypass of stop codon protein 4

Gene names

Name:	BSC4
Ordered Locus Names:	YNL269W
ORF Names:	N0670

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous

Open reading frame that exhibits genomic organization compatible with a translational readthrough-dependent mode of expression. The sequence can give a readthrough frequency of 9% when cloned in a plasmid with reporter genes. When the initial stop codon is bypassed, translation will go on to the second stop codon 321 bp downstream. This protein has no orthologs in any species other than the S.cerevisiae lineage. It may be the result of de novo origination of a protein-coding gene.

Ontologies

Keywords
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-2047140,EBI-24597
ECM4	P36156	1	EBI-2047140,EBI-2042717

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Bypass of stop codon protein 4		PRO_0000064994
Natural variations
	Natural variant	3	1	I → V in strain: XH1549. Ref.1
	Natural variant	6	1	R → Q in strain: AS2.101, AS2.1406, AS2.179, AS2.7, AS2.724, AS2.771, AS2.820 and AS2.93. Ref.1
	Natural variant	9 – 10	2	NK → T in strain: XH1549. Ref.1
	Natural variant	16	1	I → L in strain: XH1549. Ref.1
	Natural variant	19	1	K → Q in strain: XH1549. Ref.1
	Natural variant	22	1	T → S in strain: XH1549. Ref.1
	Natural variant	32	1	V → G in strain: XH1549. Ref.1
	Natural variant	42	1	S → N in strain: AS2.101, AS2.1406, AS2.179, AS2.7, AS2.724, AS2.771, AS2.820, AS2.93 and XH1549. Ref.1
	Natural variant	92	1	R → H in strain: AS2.724 and AS2.820. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53841-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F9B0C290CB317BBC	FASTA	131	15,314
	10 20 30 40 50 60 MSIVLRKSNK KNKNCITSKF YTIHIIKIST PVFRAPIAIG ESPYVEWSCL QVVFRKDMVT 70 80 90 100 110 120 KKTTFAQLIT RLNHFLCQAL KRRDSKTYIL CRTAVFGAMT PFSPRKSHIN NKLPMQPRKK 130 KIVIIYVVRF H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"De novo origination of a new protein-coding gene in Saccharomyces cerevisiae." Cai J., Zhao R., Jiang H., Wang W. Genetics 179:487-496(2008) [PubMed: 18493065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-3; GLN-6; THR-9; LEU-16; GLN-19; SER-22; GLY-32; ASN-42 AND HIS-92. Strain: AS2.101, AS2.1406, AS2.148, AS2.179, AS2.2, AS2.2079, AS2.2080, AS2.3, AS2.7, AS2.724, AS2.771, AS2.820, AS2.93 and XH1549.
[2]	"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes." Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H. Yeast 12:505-514(1996) [PubMed: 8740425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Identification of stop codon readthrough genes in Saccharomyces cerevisiae." Namy O., Duchateau-Nguyen G., Hatin I., Hermann-Le Denmat S., Termier M., Rousset J.-P. Nucleic Acids Res. 31:2289-2296(2003) [PubMed: 12711673] [Abstract] Cited for: GENE NAME.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU375912 Genomic DNA. Translation: ACB40933.1. EU375913 Genomic DNA. Translation: ACB40934.1. EU375914 Genomic DNA. Translation: ACB40935.1. EU375915 Genomic DNA. Translation: ACB40936.1. EU375916 Genomic DNA. Translation: ACB40937.1. EU375917 Genomic DNA. Translation: ACB40938.1. EU375918 Genomic DNA. Translation: ACB40939.1. EU375919 Genomic DNA. Translation: ACB40940.1. EU375920 Genomic DNA. Translation: ACB40941.1. EU375921 Genomic DNA. Translation: ACB40942.1. EU375922 Genomic DNA. Translation: ACB40943.1. EU375923 Genomic DNA. Translation: ACB40944.1. EU375924 Genomic DNA. Translation: ACB40945.1. EU375925 Genomic DNA. Translation: ACB40946.1. X92494 Genomic DNA. Translation: CAA63229.1. Z71545 Genomic DNA. Translation: CAA96176.1.
PIR	S60913.
RefSeq	NP_014130.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P53841. 2 interactions.
Genome annotation databases
Ensembl	YNL269W; YNL269W; YNL269W; Saccharomyces cerevisiae. [Genome view]
GeneID	855452.
KEGG	sce:YNL269W.
NMPDR	fig|4932.3.peg.5195.
Organism-specific databases
CYGD	YNL269w.
SGD	S000005213. BSC4.
Phylogenomic databases
OrthoDB	EOG9S7M7G
Gene expression databases
ArrayExpress	P53841.
Genevestigator	P53841.
GermOnline	YNL269W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	979364.

Entry information

Entry name

BSC4_YEAST

Accession

Primary (citable) accession number: P53841
Secondary accession number(s): B2Y304 , B2Y305, B2Y306, B2Y308

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)