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P53840

- TOF1_YEAST

UniProt

P53840 - TOF1_YEAST

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Protein
Topoisomerase 1-associated factor 1
Gene
TOF1, YNL273W, N0636
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.9 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. DNA repair Source: SGD
  2. DNA replication Source: SGD
  3. DNA replication checkpoint Source: SGD
  4. maintenance of DNA repeat elements Source: SGD
  5. meiotic nuclear division Source: UniProtKB-KW
  6. mitotic sister chromatid cohesion Source: SGD
  7. replication fork arrest Source: SGD
  8. replication fork protection Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA replication inhibitor

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Topoisomerase 1-associated factor 1
Gene namesi
Name:TOF1
Ordered Locus Names:YNL273W
ORF Names:N0636
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL273w.
SGDiS000005217. TOF1.

Subcellular locationi

Nucleus
Note: Associated with chromatin during S phase.2 Publications

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nuclear chromosome Source: SGD
  3. nuclear replication fork Source: SGD
  4. nucleus Source: SGD
  5. replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12381238Topoisomerase 1-associated factor 1
PRO_0000072620Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei626 – 6261Phosphoserine1 Publication
Modified residuei654 – 6541Phosphoserine1 Publication
Modified residuei1056 – 10561Phosphoserine1 Publication
Modified residuei1058 – 10581Phosphoserine1 Publication
Modified residuei1213 – 12131Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53840.
PaxDbiP53840.

Expressioni

Gene expression databases

GenevestigatoriP53840.

Interactioni

Subunit structurei

Component of the fork protection complex (FPC) consisting of TOF1 and CSM3. Interacts with WSS1 and ESC4.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC45Q080323EBI-28257,EBI-4292
CSM3Q046598EBI-28257,EBI-28093

Protein-protein interaction databases

BioGridi35567. 206 interactions.
DIPiDIP-4272N.
IntActiP53840. 8 interactions.
MINTiMINT-529011.
STRINGi4932.YNL273W.

Structurei

3D structure databases

ProteinModelPortaliP53840.

Family & Domainsi

Sequence similaritiesi

Belongs to the timeless family.

Phylogenomic databases

eggNOGiNOG310534.
HOGENOMiHOG000057108.
KOiK10997.
OMAiGDDCLAC.
OrthoDBiEOG7PGF01.

Family and domain databases

InterProiIPR006906. Timeless.
[Graphical view]
PfamiPF04821. TIMELESS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53840-1 [UniParc]FASTAAdd to Basket

« Hide

MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD     50
CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLLP ILIDWENKCS 100
LAAKLAKNNP DHEEFRNKAY YDKIALNCLQ LLVLMTWPLI VTEQSSSNQI 150
TLYGELKKHQ LVYKKTILSM ESGKVLRAAI RLALDVIKID RLSRTPRDNM 200
VLKLVLNFFR NVIAIEPGEF TINTKKSMPK KGITSIDTLP PNVSMDDISL 250
NTVISSFHKN KVFGFLLTLT SSLSKEFDQD FINIPLLEIM FYFTKDVNQE 300
LLFPRQFETG THSKVVNKNE SSSANNIVTS AGFELSKLLQ KEHQMRKNVI 350
KHTSARHSRF GGLLSIQTPD KTRLTVSGSQ ALVDEKIALQ KLDDSKKWNK 400
RIIKKHQSVA AEGLPNSLLN SQTGKAIFFT ESNGKHFKEF INNFIDSGFN 450
ILLHSVTNYF TTEQDRMVTL EQVEYLLFFA WFVKYQLLRS KIDNSADIKQ 500
VSEALKEVTF ILVSSLLRSA YDLKNWTVTH AGMIAFNELL NLVSRTKAAQ 550
EEDSTDIEFI VSRLFSDERI QLLSNLPKIG SKYSLQFMKS CIELTHSVLK 600
VLEQYSDDKT LVIEGKSRRQ KKFNISEGDI TKLIEEENVD RDEALDILTS 650
SLRSIEVNFQ KVQANYMTEP VIETYINFLE RFRELEDDSI KKVFSFFHRV 700
FVQAKEQALL FRFDLIILLR EMLSPDGLDR MSRSRKYVSQ FSDYFLARLK 750
KRLKKSPAWF VGLLFPPLHN SEVGFYQRYG EYNVLNNESM YAAPASQFKP 800
IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW 850
LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRALL LLIGYSIPRK 900
INEPCFLPGT VEVSDLTVSC ELVKKYLSTP FETPNGLPSS SYLLRVRSEK 950
DSFSHNEQDG WEGDDDYDYN DPYIVPDDQI LSKSDAAYFK DLDNNASDKL 1000
KGTKFSKGIA RSKKKDKRKR RKGEAKTNLP MFGDQDDERP QTVRERHGVF 1050
SKEFISDSED DEDLMNPIFF ENETYMRWLL DKNNGQLTED RYIQFAKFAA 1100
ERMNNGGVVT GDYTSLFGGS IPSIESIRAT ESSSFAPDKS LISLASHVAS 1150
EMSIFDVNNN NNNQLSDDDV NSESRNSLGS SQPSNSQNMF QSEVYSRKES 1200
TKRSLEASAA DESDEDEEAI RLFGKKSRVV LSQGDSDD 1238
Length:1,238
Mass (Da):141,120
Last modified:October 1, 1996 - v1
Checksum:iCF8FEC33EE0088E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71549 Genomic DNA. Translation: CAA96181.1.
BK006947 Genomic DNA. Translation: DAA10287.1.
PIRiS63247.
RefSeqiNP_014126.1. NM_001183111.1.

Genome annotation databases

EnsemblFungiiYNL273W; YNL273W; YNL273W.
GeneIDi855448.
KEGGisce:YNL273W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71549 Genomic DNA. Translation: CAA96181.1 .
BK006947 Genomic DNA. Translation: DAA10287.1 .
PIRi S63247.
RefSeqi NP_014126.1. NM_001183111.1.

3D structure databases

ProteinModelPortali P53840.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35567. 206 interactions.
DIPi DIP-4272N.
IntActi P53840. 8 interactions.
MINTi MINT-529011.
STRINGi 4932.YNL273W.

Proteomic databases

MaxQBi P53840.
PaxDbi P53840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL273W ; YNL273W ; YNL273W .
GeneIDi 855448.
KEGGi sce:YNL273W.

Organism-specific databases

CYGDi YNL273w.
SGDi S000005217. TOF1.

Phylogenomic databases

eggNOGi NOG310534.
HOGENOMi HOG000057108.
KOi K10997.
OMAi GDDCLAC.
OrthoDBi EOG7PGF01.

Enzyme and pathway databases

BioCyci YEAST:G3O-33267-MONOMER.

Miscellaneous databases

NextBioi 979352.

Gene expression databases

Genevestigatori P53840.

Family and domain databases

InterProi IPR006906. Timeless.
[Graphical view ]
Pfami PF04821. TIMELESS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae."
    Park H., Sternglanz R.
    Yeast 15:35-41(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae."
    Foss E.J.
    Genetics 157:567-577(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex."
    Katou Y., Kanoh Y., Bando M., Noguchi H., Tanaka H., Ashikari T., Sugimoto K., Shirahige K.
    Nature 424:1078-1083(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Identification of protein complexes required for efficient sister chromatid cohesion."
    Mayer M.L., Pot I., Chang M., Xu H., Aneliunas V., Kwok T., Newitt R., Aebersold R., Boone C., Brown G.W., Hieter P.
    Mol. Biol. Cell 15:1736-1745(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CSM3.
  9. "Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response."
    O'Neill B.M., Hanway D., Winzeler E.A., Romesberg F.E.
    Nucleic Acids Res. 32:6519-6530(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH WSS1.
  10. "Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex."
    Nedelcheva M.N., Roguev A., Dolapchiev L.B., Shevchenko A., Taskov H.B., Shevchenko A., Stewart A.F., Stoynov S.S.
    J. Mol. Biol. 347:509-521(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE FPC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53."
    Tourriere H., Versini G., Cordon-Preciado V., Alabert C., Pasero P.
    Mol. Cell 19:699-706(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Disruption of mechanisms that prevent rereplication triggers a DNA damage response."
    Archambault V., Ikui A.E., Drapkin B.J., Cross F.R.
    Mol. Cell. Biol. 25:6707-6721(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Esc4/Rtt107 and the control of recombination during replication."
    Chin J.K., Bashkirov V.I., Heyer W.-D., Romesberg F.E.
    DNA Repair 5:618-628(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ESC4.
  14. "Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage."
    Redon C., Pilch D.R., Bonner W.M.
    Genetics 172:67-76(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE FPC COMPLEX.
  15. "The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae."
    Mohanty B.K., Bairwa N.K., Bastia D.
    Proc. Natl. Acad. Sci. U.S.A. 103:897-902(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE FPC COMPLEX.
  16. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626; SER-654 AND SER-1213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1056; SER-1058 AND SER-1213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTOF1_YEAST
AccessioniPrimary (citable) accession number: P53840
Secondary accession number(s): D6W0S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi