Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Topoisomerase 1-associated factor 1

Gene

TOF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.9 Publications

GO - Biological processi

  • DNA repair Source: SGD
  • DNA replication Source: SGD
  • DNA replication checkpoint Source: SGD
  • maintenance of DNA repeat elements Source: SGD
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic sister chromatid cohesion Source: SGD
  • replication fork arrest Source: SGD
  • replication fork protection Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

DNA replication inhibitor

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33267-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Topoisomerase 1-associated factor 1
Gene namesi
Name:TOF1
Ordered Locus Names:YNL273W
ORF Names:N0636
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL273W.
SGDiS000005217. TOF1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nuclear chromosome Source: SGD
  • nuclear replication fork Source: SGD
  • nucleus Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726201 – 1238Topoisomerase 1-associated factor 1Add BLAST1238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei626PhosphoserineCombined sources1
Modified residuei654PhosphoserineCombined sources1
Modified residuei1056PhosphoserineCombined sources1
Modified residuei1058PhosphoserineCombined sources1
Modified residuei1213PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53840.
PRIDEiP53840.

PTM databases

iPTMnetiP53840.

Interactioni

Subunit structurei

Component of the fork protection complex (FPC) consisting of TOF1 and CSM3. Interacts with WSS1 and ESC4.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC45Q080323EBI-28257,EBI-4292
CSM3Q046598EBI-28257,EBI-28093

Protein-protein interaction databases

BioGridi35567. 211 interactors.
DIPiDIP-4272N.
IntActiP53840. 8 interactors.
MINTiMINT-529011.

Structurei

3D structure databases

ProteinModelPortaliP53840.
SMRiP53840.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the timeless family.Curated

Phylogenomic databases

HOGENOMiHOG000057108.
InParanoidiP53840.
KOiK10997.
OMAiGDDCLAC.
OrthoDBiEOG092C0HGP.

Family and domain databases

InterProiIPR006906. Timeless.
[Graphical view]
PfamiPF04821. TIMELESS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD
60 70 80 90 100
CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLLP ILIDWENKCS
110 120 130 140 150
LAAKLAKNNP DHEEFRNKAY YDKIALNCLQ LLVLMTWPLI VTEQSSSNQI
160 170 180 190 200
TLYGELKKHQ LVYKKTILSM ESGKVLRAAI RLALDVIKID RLSRTPRDNM
210 220 230 240 250
VLKLVLNFFR NVIAIEPGEF TINTKKSMPK KGITSIDTLP PNVSMDDISL
260 270 280 290 300
NTVISSFHKN KVFGFLLTLT SSLSKEFDQD FINIPLLEIM FYFTKDVNQE
310 320 330 340 350
LLFPRQFETG THSKVVNKNE SSSANNIVTS AGFELSKLLQ KEHQMRKNVI
360 370 380 390 400
KHTSARHSRF GGLLSIQTPD KTRLTVSGSQ ALVDEKIALQ KLDDSKKWNK
410 420 430 440 450
RIIKKHQSVA AEGLPNSLLN SQTGKAIFFT ESNGKHFKEF INNFIDSGFN
460 470 480 490 500
ILLHSVTNYF TTEQDRMVTL EQVEYLLFFA WFVKYQLLRS KIDNSADIKQ
510 520 530 540 550
VSEALKEVTF ILVSSLLRSA YDLKNWTVTH AGMIAFNELL NLVSRTKAAQ
560 570 580 590 600
EEDSTDIEFI VSRLFSDERI QLLSNLPKIG SKYSLQFMKS CIELTHSVLK
610 620 630 640 650
VLEQYSDDKT LVIEGKSRRQ KKFNISEGDI TKLIEEENVD RDEALDILTS
660 670 680 690 700
SLRSIEVNFQ KVQANYMTEP VIETYINFLE RFRELEDDSI KKVFSFFHRV
710 720 730 740 750
FVQAKEQALL FRFDLIILLR EMLSPDGLDR MSRSRKYVSQ FSDYFLARLK
760 770 780 790 800
KRLKKSPAWF VGLLFPPLHN SEVGFYQRYG EYNVLNNESM YAAPASQFKP
810 820 830 840 850
IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW
860 870 880 890 900
LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRALL LLIGYSIPRK
910 920 930 940 950
INEPCFLPGT VEVSDLTVSC ELVKKYLSTP FETPNGLPSS SYLLRVRSEK
960 970 980 990 1000
DSFSHNEQDG WEGDDDYDYN DPYIVPDDQI LSKSDAAYFK DLDNNASDKL
1010 1020 1030 1040 1050
KGTKFSKGIA RSKKKDKRKR RKGEAKTNLP MFGDQDDERP QTVRERHGVF
1060 1070 1080 1090 1100
SKEFISDSED DEDLMNPIFF ENETYMRWLL DKNNGQLTED RYIQFAKFAA
1110 1120 1130 1140 1150
ERMNNGGVVT GDYTSLFGGS IPSIESIRAT ESSSFAPDKS LISLASHVAS
1160 1170 1180 1190 1200
EMSIFDVNNN NNNQLSDDDV NSESRNSLGS SQPSNSQNMF QSEVYSRKES
1210 1220 1230
TKRSLEASAA DESDEDEEAI RLFGKKSRVV LSQGDSDD
Length:1,238
Mass (Da):141,120
Last modified:October 1, 1996 - v1
Checksum:iCF8FEC33EE0088E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71549 Genomic DNA. Translation: CAA96181.1.
BK006947 Genomic DNA. Translation: DAA10287.1.
PIRiS63247.
RefSeqiNP_014126.1. NM_001183111.1.

Genome annotation databases

EnsemblFungiiYNL273W; YNL273W; YNL273W.
GeneIDi855448.
KEGGisce:YNL273W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71549 Genomic DNA. Translation: CAA96181.1.
BK006947 Genomic DNA. Translation: DAA10287.1.
PIRiS63247.
RefSeqiNP_014126.1. NM_001183111.1.

3D structure databases

ProteinModelPortaliP53840.
SMRiP53840.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35567. 211 interactors.
DIPiDIP-4272N.
IntActiP53840. 8 interactors.
MINTiMINT-529011.

PTM databases

iPTMnetiP53840.

Proteomic databases

MaxQBiP53840.
PRIDEiP53840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL273W; YNL273W; YNL273W.
GeneIDi855448.
KEGGisce:YNL273W.

Organism-specific databases

EuPathDBiFungiDB:YNL273W.
SGDiS000005217. TOF1.

Phylogenomic databases

HOGENOMiHOG000057108.
InParanoidiP53840.
KOiK10997.
OMAiGDDCLAC.
OrthoDBiEOG092C0HGP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33267-MONOMER.

Miscellaneous databases

PROiP53840.

Family and domain databases

InterProiIPR006906. Timeless.
[Graphical view]
PfamiPF04821. TIMELESS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOF1_YEAST
AccessioniPrimary (citable) accession number: P53840
Secondary accession number(s): D6W0S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.