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P53840

- TOF1_YEAST

UniProt

P53840 - TOF1_YEAST

Protein

Topoisomerase 1-associated factor 1

Gene

TOF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.9 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. DNA repair Source: SGD
    2. DNA replication Source: SGD
    3. DNA replication checkpoint Source: SGD
    4. maintenance of DNA repeat elements Source: SGD
    5. meiotic nuclear division Source: UniProtKB-KW
    6. mitotic sister chromatid cohesion Source: SGD
    7. replication fork arrest Source: SGD
    8. replication fork protection Source: SGD

    Keywords - Molecular functioni

    DNA replication inhibitor

    Keywords - Biological processi

    Cell cycle, DNA damage, DNA repair, Meiosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33267-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Topoisomerase 1-associated factor 1
    Gene namesi
    Name:TOF1
    Ordered Locus Names:YNL273W
    ORF Names:N0636
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL273w.
    SGDiS000005217. TOF1.

    Subcellular locationi

    Nucleus 2 Publications
    Note: Associated with chromatin during S phase.

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nuclear chromosome Source: SGD
    3. nuclear replication fork Source: SGD
    4. nucleus Source: SGD
    5. replication fork protection complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12381238Topoisomerase 1-associated factor 1PRO_0000072620Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei626 – 6261Phosphoserine1 Publication
    Modified residuei654 – 6541Phosphoserine1 Publication
    Modified residuei1056 – 10561Phosphoserine1 Publication
    Modified residuei1058 – 10581Phosphoserine1 Publication
    Modified residuei1213 – 12131Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53840.
    PaxDbiP53840.

    Expressioni

    Gene expression databases

    GenevestigatoriP53840.

    Interactioni

    Subunit structurei

    Component of the fork protection complex (FPC) consisting of TOF1 and CSM3. Interacts with WSS1 and ESC4.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC45Q080323EBI-28257,EBI-4292
    CSM3Q046598EBI-28257,EBI-28093

    Protein-protein interaction databases

    BioGridi35567. 206 interactions.
    DIPiDIP-4272N.
    IntActiP53840. 8 interactions.
    MINTiMINT-529011.
    STRINGi4932.YNL273W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53840.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the timeless family.Curated

    Phylogenomic databases

    eggNOGiNOG310534.
    HOGENOMiHOG000057108.
    KOiK10997.
    OMAiGDDCLAC.
    OrthoDBiEOG7PGF01.

    Family and domain databases

    InterProiIPR006906. Timeless.
    [Graphical view]
    PfamiPF04821. TIMELESS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53840-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD     50
    CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLLP ILIDWENKCS 100
    LAAKLAKNNP DHEEFRNKAY YDKIALNCLQ LLVLMTWPLI VTEQSSSNQI 150
    TLYGELKKHQ LVYKKTILSM ESGKVLRAAI RLALDVIKID RLSRTPRDNM 200
    VLKLVLNFFR NVIAIEPGEF TINTKKSMPK KGITSIDTLP PNVSMDDISL 250
    NTVISSFHKN KVFGFLLTLT SSLSKEFDQD FINIPLLEIM FYFTKDVNQE 300
    LLFPRQFETG THSKVVNKNE SSSANNIVTS AGFELSKLLQ KEHQMRKNVI 350
    KHTSARHSRF GGLLSIQTPD KTRLTVSGSQ ALVDEKIALQ KLDDSKKWNK 400
    RIIKKHQSVA AEGLPNSLLN SQTGKAIFFT ESNGKHFKEF INNFIDSGFN 450
    ILLHSVTNYF TTEQDRMVTL EQVEYLLFFA WFVKYQLLRS KIDNSADIKQ 500
    VSEALKEVTF ILVSSLLRSA YDLKNWTVTH AGMIAFNELL NLVSRTKAAQ 550
    EEDSTDIEFI VSRLFSDERI QLLSNLPKIG SKYSLQFMKS CIELTHSVLK 600
    VLEQYSDDKT LVIEGKSRRQ KKFNISEGDI TKLIEEENVD RDEALDILTS 650
    SLRSIEVNFQ KVQANYMTEP VIETYINFLE RFRELEDDSI KKVFSFFHRV 700
    FVQAKEQALL FRFDLIILLR EMLSPDGLDR MSRSRKYVSQ FSDYFLARLK 750
    KRLKKSPAWF VGLLFPPLHN SEVGFYQRYG EYNVLNNESM YAAPASQFKP 800
    IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW 850
    LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRALL LLIGYSIPRK 900
    INEPCFLPGT VEVSDLTVSC ELVKKYLSTP FETPNGLPSS SYLLRVRSEK 950
    DSFSHNEQDG WEGDDDYDYN DPYIVPDDQI LSKSDAAYFK DLDNNASDKL 1000
    KGTKFSKGIA RSKKKDKRKR RKGEAKTNLP MFGDQDDERP QTVRERHGVF 1050
    SKEFISDSED DEDLMNPIFF ENETYMRWLL DKNNGQLTED RYIQFAKFAA 1100
    ERMNNGGVVT GDYTSLFGGS IPSIESIRAT ESSSFAPDKS LISLASHVAS 1150
    EMSIFDVNNN NNNQLSDDDV NSESRNSLGS SQPSNSQNMF QSEVYSRKES 1200
    TKRSLEASAA DESDEDEEAI RLFGKKSRVV LSQGDSDD 1238
    Length:1,238
    Mass (Da):141,120
    Last modified:October 1, 1996 - v1
    Checksum:iCF8FEC33EE0088E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71549 Genomic DNA. Translation: CAA96181.1.
    BK006947 Genomic DNA. Translation: DAA10287.1.
    PIRiS63247.
    RefSeqiNP_014126.1. NM_001183111.1.

    Genome annotation databases

    EnsemblFungiiYNL273W; YNL273W; YNL273W.
    GeneIDi855448.
    KEGGisce:YNL273W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71549 Genomic DNA. Translation: CAA96181.1 .
    BK006947 Genomic DNA. Translation: DAA10287.1 .
    PIRi S63247.
    RefSeqi NP_014126.1. NM_001183111.1.

    3D structure databases

    ProteinModelPortali P53840.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35567. 206 interactions.
    DIPi DIP-4272N.
    IntActi P53840. 8 interactions.
    MINTi MINT-529011.
    STRINGi 4932.YNL273W.

    Proteomic databases

    MaxQBi P53840.
    PaxDbi P53840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL273W ; YNL273W ; YNL273W .
    GeneIDi 855448.
    KEGGi sce:YNL273W.

    Organism-specific databases

    CYGDi YNL273w.
    SGDi S000005217. TOF1.

    Phylogenomic databases

    eggNOGi NOG310534.
    HOGENOMi HOG000057108.
    KOi K10997.
    OMAi GDDCLAC.
    OrthoDBi EOG7PGF01.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33267-MONOMER.

    Miscellaneous databases

    NextBioi 979352.

    Gene expression databases

    Genevestigatori P53840.

    Family and domain databases

    InterProi IPR006906. Timeless.
    [Graphical view ]
    Pfami PF04821. TIMELESS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae."
      Park H., Sternglanz R.
      Yeast 15:35-41(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae."
      Foss E.J.
      Genetics 157:567-577(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex."
      Katou Y., Kanoh Y., Bando M., Noguchi H., Tanaka H., Ashikari T., Sugimoto K., Shirahige K.
      Nature 424:1078-1083(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Identification of protein complexes required for efficient sister chromatid cohesion."
      Mayer M.L., Pot I., Chang M., Xu H., Aneliunas V., Kwok T., Newitt R., Aebersold R., Boone C., Brown G.W., Hieter P.
      Mol. Biol. Cell 15:1736-1745(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CSM3.
    9. "Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response."
      O'Neill B.M., Hanway D., Winzeler E.A., Romesberg F.E.
      Nucleic Acids Res. 32:6519-6530(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH WSS1.
    10. "Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex."
      Nedelcheva M.N., Roguev A., Dolapchiev L.B., Shevchenko A., Taskov H.B., Shevchenko A., Stewart A.F., Stoynov S.S.
      J. Mol. Biol. 347:509-521(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE FPC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    11. "Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53."
      Tourriere H., Versini G., Cordon-Preciado V., Alabert C., Pasero P.
      Mol. Cell 19:699-706(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Disruption of mechanisms that prevent rereplication triggers a DNA damage response."
      Archambault V., Ikui A.E., Drapkin B.J., Cross F.R.
      Mol. Cell. Biol. 25:6707-6721(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Esc4/Rtt107 and the control of recombination during replication."
      Chin J.K., Bashkirov V.I., Heyer W.-D., Romesberg F.E.
      DNA Repair 5:618-628(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ESC4.
    14. "Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage."
      Redon C., Pilch D.R., Bonner W.M.
      Genetics 172:67-76(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE FPC COMPLEX.
    15. "The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae."
      Mohanty B.K., Bairwa N.K., Bastia D.
      Proc. Natl. Acad. Sci. U.S.A. 103:897-902(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE FPC COMPLEX.
    16. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626; SER-654 AND SER-1213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1056; SER-1058 AND SER-1213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTOF1_YEAST
    AccessioniPrimary (citable) accession number: P53840
    Secondary accession number(s): D6W0S1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 952 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3