P53840 (TOF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Topoisomerase 1-associated factor 1

Gene names

Name:	TOF1
Ordered Locus Names:	YNL273W
ORF Names:	N0636

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase. Ref.4 Ref.5 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.15
Subunit structure	Component of the fork protection complex (FPC) consisting of TOF1 and CSM3. Interacts with WSS1 and ESC4. Ref.8 Ref.9 Ref.10 Ref.13
Subcellular location	Nucleus. Note: Associated with chromatin during S phase. Ref.5 Ref.6
Miscellaneous	Present with 952 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the timeless family.

Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair Meiosis
Cellular component	Nucleus
Molecular function	DNA replication inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from mutant phenotype Ref.14. Source: SGD DNA replication checkpoint Inferred from physical interaction Ref.5. Source: SGD maintenance of DNA repeat elements Inferred from mutant phenotype PubMed 18321795. Source: SGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic sister chromatid cohesion Inferred from mutant phenotype Ref.8PubMed 17483413. Source: SGD replication fork arrest Inferred from mutant phenotype PubMed 16103218Ref.15PubMed 17652453. Source: SGD replication fork protection Inferred from mutant phenotype Ref.15PubMed 18632578. Source: SGD
Cellular_component	replication fork protection complex Inferred from direct assay PubMed 16531994. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC45	Q08032	3	EBI-28257,EBI-4292
CSM3	Q04659	8	EBI-28257,EBI-28093

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1238	1238	Topoisomerase 1-associated factor 1		PRO_0000072620
Amino acid modifications
	Modified residue	626	1	Phosphoserine Ref.17 Ref.18
	Modified residue	654	1	Phosphoserine Ref.18
	Modified residue	1134	1	Phosphoserine Ref.18
	Modified residue	1213	1	Phosphoserine Ref.16 Ref.18

Sequences

	Sequence		Length	Mass (Da)
	P53840 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CF8FEC33EE0088E9	FASTA	1,238	141,120
	10 20 30 40 50 60 MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD CLACLKDLKR 70 80 90 100 110 120 WFKLVDDQQK RWDVAMAVAE YRILTDDLLP ILIDWENKCS LAAKLAKNNP DHEEFRNKAY 130 140 150 160 170 180 YDKIALNCLQ LLVLMTWPLI VTEQSSSNQI TLYGELKKHQ LVYKKTILSM ESGKVLRAAI 190 200 210 220 230 240 RLALDVIKID RLSRTPRDNM VLKLVLNFFR NVIAIEPGEF TINTKKSMPK KGITSIDTLP 250 260 270 280 290 300 PNVSMDDISL NTVISSFHKN KVFGFLLTLT SSLSKEFDQD FINIPLLEIM FYFTKDVNQE 310 320 330 340 350 360 LLFPRQFETG THSKVVNKNE SSSANNIVTS AGFELSKLLQ KEHQMRKNVI KHTSARHSRF 370 380 390 400 410 420 GGLLSIQTPD KTRLTVSGSQ ALVDEKIALQ KLDDSKKWNK RIIKKHQSVA AEGLPNSLLN 430 440 450 460 470 480 SQTGKAIFFT ESNGKHFKEF INNFIDSGFN ILLHSVTNYF TTEQDRMVTL EQVEYLLFFA 490 500 510 520 530 540 WFVKYQLLRS KIDNSADIKQ VSEALKEVTF ILVSSLLRSA YDLKNWTVTH AGMIAFNELL 550 560 570 580 590 600 NLVSRTKAAQ EEDSTDIEFI VSRLFSDERI QLLSNLPKIG SKYSLQFMKS CIELTHSVLK 610 620 630 640 650 660 VLEQYSDDKT LVIEGKSRRQ KKFNISEGDI TKLIEEENVD RDEALDILTS SLRSIEVNFQ 670 680 690 700 710 720 KVQANYMTEP VIETYINFLE RFRELEDDSI KKVFSFFHRV FVQAKEQALL FRFDLIILLR 730 740 750 760 770 780 EMLSPDGLDR MSRSRKYVSQ FSDYFLARLK KRLKKSPAWF VGLLFPPLHN SEVGFYQRYG 790 800 810 820 830 840 EYNVLNNESM YAAPASQFKP IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI 850 860 870 880 890 900 THTLDIFKSW LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRALL LLIGYSIPRK 910 920 930 940 950 960 INEPCFLPGT VEVSDLTVSC ELVKKYLSTP FETPNGLPSS SYLLRVRSEK DSFSHNEQDG 970 980 990 1000 1010 1020 WEGDDDYDYN DPYIVPDDQI LSKSDAAYFK DLDNNASDKL KGTKFSKGIA RSKKKDKRKR 1030 1040 1050 1060 1070 1080 RKGEAKTNLP MFGDQDDERP QTVRERHGVF SKEFISDSED DEDLMNPIFF ENETYMRWLL 1090 1100 1110 1120 1130 1140 DKNNGQLTED RYIQFAKFAA ERMNNGGVVT GDYTSLFGGS IPSIESIRAT ESSSFAPDKS 1150 1160 1170 1180 1190 1200 LISLASHVAS EMSIFDVNNN NNNQLSDDDV NSESRNSLGS SQPSNSQNMF QSEVYSRKES 1210 1220 1230 TKRSLEASAA DESDEDEEAI RLFGKKSRVV LSQGDSDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae." Park H., Sternglanz R. Yeast 15:35-41(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[4]	"Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae." Foss E.J. Genetics 157:567-577(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex." Katou Y., Kanoh Y., Bando M., Noguchi H., Tanaka H., Ashikari T., Sugimoto K., Shirahige K. Nature 424:1078-1083(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Identification of protein complexes required for efficient sister chromatid cohesion." Mayer M.L., Pot I., Chang M., Xu H., Aneliunas V., Kwok T., Newitt R., Aebersold R., Boone C., Brown G.W., Hieter P. Mol. Biol. Cell 15:1736-1745(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CSM3.
[9]	"Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response." O'Neill B.M., Hanway D., Winzeler E.A., Romesberg F.E. Nucleic Acids Res. 32:6519-6530(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH WSS1.
[10]	"Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex." Nedelcheva M.N., Roguev A., Dolapchiev L.B., Shevchenko A., Taskov H.B., Shevchenko A., Stewart A.F., Stoynov S.S. J. Mol. Biol. 347:509-521(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE FPC COMPLEX, MASS SPECTROMETRY.
[11]	"Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53." Tourriere H., Versini G., Cordon-Preciado V., Alabert C., Pasero P. Mol. Cell 19:699-706(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Disruption of mechanisms that prevent rereplication triggers a DNA damage response." Archambault V., Ikui A.E., Drapkin B.J., Cross F.R. Mol. Cell. Biol. 25:6707-6721(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Esc4/Rtt107 and the control of recombination during replication." Chin J.K., Bashkirov V.I., Heyer W.-D., Romesberg F.E. DNA Repair 5:618-628(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ESC4.
[14]	"Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage." Redon C., Pilch D.R., Bonner W.M. Genetics 172:67-76(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE FPC COMPLEX.
[15]	"The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae." Mohanty B.K., Bairwa N.K., Bastia D. Proc. Natl. Acad. Sci. U.S.A. 103:897-902(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE FPC COMPLEX.
[16]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213, MASS SPECTROMETRY. Strain: ADR376.
[17]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626, MASS SPECTROMETRY.
[18]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-626; SER-654; SER-1134 AND SER-1213, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71549 Genomic DNA. Translation: CAA96181.1. BK006947 Genomic DNA. Translation: DAA10287.1.
PIR	S63247.
RefSeq	NP_014126.1. NM_001183111.1.
3D structure databases
ProteinModelPortal	P53840.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4272N.
IntAct	P53840. 7 interactions.
MINT	MINT-529011.
STRING	4932.YNL273W.
Proteomic databases
PaxDb	P53840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL273W; YNL273W; YNL273W.
GeneID	855448.
KEGG	sce:YNL273W.
Organism-specific databases
CYGD	YNL273w.
SGD	S000005217. TOF1.
Phylogenomic databases
eggNOG	NOG310534.
GeneTree	ENSGT00390000015124.
HOGENOM	HOG000057108.
KO	K10997.
OMA	GDDCLAC.
OrthoDB	EOG45MRDF.
Gene expression databases
Genevestigator	P53840.
GermOnline	YNL273W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006906. Timeless. [Graphical view]
Pfam	PF04821. TIMELESS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979352.

Entry information

Entry name

TOF1_YEAST

Accession

Primary (citable) accession number: P53840
Secondary accession number(s): D6W0S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program