Reviewed, UniProtKB/Swiss-Prot P53838 (BOR1_YEAST)

Names and origin

Protein names

Recommended name:
    Boron transporter 1

Gene names

Name:	BOR1
Ordered Locus Names:	YNL275W
ORF Names:	N0626

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions in boric acid/borate export across the plasma membrane, and thereby protects yeast cells from boron toxicity. Involved in the trafficking of proteins to the vacuole. Ref.2 Ref.4 Ref.5 Ref.7 Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein.
Miscellaneous	Present with 195 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Ontologies

Keywords
Biological process	Anion exchange Ion transport Transport
Cellular component	Cell membrane Membrane Vacuole
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anion transport Inferred from electronic annotation. Source: InterPro boron transport Ref.4 Ref.7 Inferred from direct assay. Source: SGD protein targeting to vacuole Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	fungal-type vacuole Ref.5 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Ref.2 Inferred from direct assay. Source: SGD plasma membrane Ref.2 Inferred from direct assay. Source: SGD
Molecular function	boron binding Inferred from direct assay. Source: SGD boron transporter activity Ref.4 Ref.7 Inferred from direct assay. Source: SGD inorganic anion exchanger activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Boron transporter 1		PRO_0000079243
Regions
	Topological domain	1 – 84	84	Cytoplasmic Potential
	Transmembrane	85 – 105	21	Potential
	Topological domain	106 – 116	11	Extracellular Potential
	Transmembrane	117 – 134	18	Potential
	Topological domain	135 – 140	6	Cytoplasmic Potential
	Transmembrane	141 – 160	20	Potential
	Topological domain	161 – 165	5	Extracellular Potential
	Transmembrane	166 – 186	21	Potential
	Topological domain	187 – 192	6	Cytoplasmic Potential
	Transmembrane	193 – 213	21	Potential
	Topological domain	214 – 235	22	Extracellular Potential
	Transmembrane	236 – 256	21	Potential
	Topological domain	257 – 274	18	Cytoplasmic Potential
	Transmembrane	275 – 295	21	Potential
	Topological domain	296 – 329	34	Extracellular Potential
	Transmembrane	330 – 350	21	Potential
	Topological domain	351 – 373	23	Cytoplasmic Potential
	Transmembrane	374 – 394	21	Potential
	Topological domain	395 – 438	44	Extracellular Potential
	Transmembrane	439 – 459	21	Potential
	Topological domain	460 – 495	36	Cytoplasmic Potential
	Transmembrane	496 – 516	21	Potential
	Topological domain	517 – 518	2	Extracellular Potential
	Transmembrane	519 – 539	21	Potential
	Topological domain	540 – 576	37	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P53838-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4EA3FFC89F66307A	FASTA	576	65,028
	10 20 30 40 50 60 MSNESTRVTV SRGCTASDEC AQALERTNDE LDRESSVSES RSDEESHEKL SRRRFPTLGI 70 80 90 100 110 120 GIWLDLKDRI PYYKSDWVDA FNYRVIPSIV DTYFNNLLPA IAFAQDMFDR TDNSYGVNEV 130 140 150 160 170 180 LLSSAMAGIV FGVLGGQPLC IVGVTGPISI FNYTVYEIIK PLNTSYFGFM FWICMWSMIF 190 200 210 220 230 240 HLVLAFTNAV CLLQYVTTFP CDIFGLFINV VYIQKGIQIL TRQFSAKSGE KSVQDGFASV 250 260 270 280 290 300 VVALVMTAFG LFFKLFHYYP LFSHRIRTFI SDYSTALSVL FWSSFTHFGG YLHDVKFKKL 310 320 330 340 350 360 PITKAFFPTS KVNRPQNTWL AYEPIPVKDV FIALPFGIFL TILFYFDHNV SSLMAQRHQY 370 380 390 400 410 420 KLKKPSSFHY DFALLGLTTC ISGVLGIPAP NGLIPQAPLH TETLLVRDSN QKVISCVEQR 430 440 450 460 470 480 FTNTFQGLMI LGTMTRPLLV CLGEIPQAVL SGLFFIMGIN GLMTNSIIQR LVFLFSDPNR 490 500 510 520 530 540 RDNTSPLMKV SKKSMLIFLS FSLTGFAGEF AITNTIAAIG FPLVLLLSVL VSFSFAYIFP 550 560 570 TEELKILDTN VAQKFTIKNL LLENIRDAKF CDKHED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Expression and characterization of the anion transporter homologue YNL275w in Saccharomyces cerevisiae." Zhao R., Reithmeier R.A.F. Am. J. Physiol. 281:C33-C45(2001) [PubMed: 11401825] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae." Nozawa A., Takano J., Kobayashi M., von Wiren N., Fujiwara T. FEMS Microbiol. Lett. 262:216-222(2006) [PubMed: 16923078] [Abstract] Cited for: FUNCTION.
[5]	"Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast." Decker B.L., Wickner W.T. J. Biol. Chem. 281:14523-14528(2006) [PubMed: 16565073] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[7]	"Transport and regulatory characteristics of the yeast bicarbonate transporter homolog Bor1p." Jennings M.L., Howren T.R., Cui J., Winters M., Hannigan R. Am. J. Physiol. 293:C468-C476(2007) [PubMed: 17459946] [Abstract] Cited for: FUNCTION.
[8]	"Saccharomyces cerevisiae Bor1p is a boron exporter and a key determinant of boron tolerance." Takano J., Kobayashi M., Noda Y., Fujiwara T. FEMS Microbiol. Lett. 267:230-235(2007) [PubMed: 17166224] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71551 Genomic DNA. Translation: CAA96183.1.
PIR	S63249.
RefSeq	NP_014124.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:8001N.
IntAct	P53838. 1 interaction.
STRING	P53838.
Protein family/group databases
TCDB	2.A.31.3.2. anion exchanger (AE) family.
Genome annotation databases
Ensembl	YNL275W; YNL275W; YNL275W; Saccharomyces cerevisiae. [Genome view]
GeneID	855446.
KEGG	sce:YNL275W.
NMPDR	fig|4932.3.peg.5189.
Organism-specific databases
CYGD	YNL275w.
SGD	S000005219. BOR1.
Phylogenomic databases
HOGENOM	P53838.
OMA	YDFALLG
OrthoDB	EOG9XKWQV
Gene expression databases
ArrayExpress	P53838.
Genevestigator	P53838.
GermOnline	YNL275W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011531. HCO3_transpt_C. IPR003020. HCO3_transpt_euk. [Graphical view]
PANTHER	PTHR11453. HCO3_transpt_euk. 1 hit.
Pfam	PF00955. HCO3_cotransp. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979346.

Entry information

Entry name

BOR1_YEAST

Accession

Primary (citable) accession number: P53838

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)