Reviewed, UniProtKB/Swiss-Prot P53836 (CA120_YEAST)

Names and origin

Protein names

Recommended name:
    CCR4-NOT transcriptional complex subunit CAF120
Alternative name(s):
    120 kDa CCR4-associated factor

Gene names

Name:	CAF120
Ordered Locus Names:	YNL278W
ORF Names:	N0610

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1060 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.
Subunit structure	Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, CAF120, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.
Subcellular location	Cytoplasm. Nucleus. Bud neck Ref.3.
Miscellaneous	Present with 1380 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the CAF120 family. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Ref.2 Inferred from physical interaction. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	CCR4-NOT complex Ref.2 Inferred from physical interaction. Source: SGD cellular bud neck Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1060	1060	CCR4-NOT transcriptional complex subunit CAF120		PRO_0000203378
Regions
	Domain	75 – 204	130	PH
	Compositional bias	886 – 1030	145	Pro-rich
Amino acid modifications
	Modified residue	491	1	Phosphoserine Ref.7
	Modified residue	493	1	Phosphoserine Ref.7
	Modified residue	494	1	Phosphoserine Ref.7
	Modified residue	505	1	Phosphoserine Ref.7
	Modified residue	510	1	Phosphoserine Ref.5
	Modified residue	518	1	Phosphoserine Ref.5
	Modified residue	524	1	Phosphothreonine Ref.7
	Modified residue	538	1	Phosphoserine Ref.5 Ref.6
	Modified residue	556	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53836-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CFB3F2D0B268E15B	FASTA	1,060	118,280
	10 20 30 40 50 60 MRIFSGDNKV VDSLASNPGL MSPSNFGGDF GSRLKVNVTS KKKLNDSSPT SPMESSPVSP 70 80 90 100 110 120 ELVPILTLLN AHTHRRYHEG VFLILQDLNN NGTHAARKWK DVYGVLLGTQ LALWDAKELA 130 140 150 160 170 180 EFTDPSCPVS EKKLKEVASK PTYINLTDAT LRTLDNSDNI VMECGKNLTN ALVVSTTLKN 190 200 210 220 230 240 RYFLQFGNKE SFNAWNSAIR LCLYECSSLQ EAYTGAFISS RGAKLGDIRI LLTNRKYDYK 250 260 270 280 290 300 DWVSVRFGAG MPWKRCYAVI SQSSSKKKGH FGEINLYEND KKVKKNHAMA TIVEAKALYA 310 320 330 340 350 360 VYPSSPKLID SSTIIKVVGS VKFEKKESAQ EKDVFIMPEK HQAVPSYDTI IRFLIPAMDT 370 380 390 400 410 420 FKLYGRPEKL LSSKNDPHSL LFGLPVLPHI YYLEVEDLLP LTNSVSSLHW SNNEWKEHIS 430 440 450 460 470 480 DILQRKIAQG YCGCNSTSNI TSPLPSPFLG SADLFERADG VLSPKLSYGS KSSSNNSSKN 490 500 510 520 530 540 SLPKRERVKL SSSSEQDLNN SDSPSIKRKS PPLVISESPH KVHTPTDASF RTRVTEGSPY 550 560 570 580 590 600 AKQRHPKPFA SSVNDSPSDR AKSRTVPYNN NDRKATTPEK FERGETSCGK NVDESLEKVR 610 620 630 640 650 660 NMKLEIPESN FDKFMTDKNL LSVDSKCSNE KKLSVESDLS AIYEKYSNGP FGHTEGLNGS 670 680 690 700 710 720 SDETYLRFQR ASVHSESNYN SRKSFTPSDF SNGNEEEHAV LQELNSLTQR INELGMESIN 730 740 750 760 770 780 SNSDSDRING SYSQVDFGNN NDEDDMNLFD PDFMAQDQLR AEERDYNKDD RTPLAKVPAA 790 800 810 820 830 840 FQSTGLGITP DDDIERQYIT EHRSRHEVPK RSPEKPSNPL EIGNPYAKPG TRLNTTHTHS 850 860 870 880 890 900 KTDRSITPQR GQPVPSGQQI SSYVQPANIN SPNKMYGANN SAMGSPRNPK TRAPPGPYNQ 910 920 930 940 950 960 GWNNRPSPSN IYQRPHPSDT QPQAYHLPGN PYSTGNRPNM QAQYHPQQVP MPILQQPNRP 970 980 990 1000 1010 1020 YQPYAMNTHM GSPGGYAGAA PPFQPANVNY NTRPQQPWPT PNSPSAHYRP PPNLNQPQNG 1030 1040 1050 1060 SAGYYRPPAP QLQNSQARPQ KKDGFSQFMP SATTKNPYAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex." Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L. J. Mol. Biol. 314:683-694(2001) [PubMed: 11733989] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510; SER-518; SER-538 AND SER-556, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-538, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491; SER-493; SER-494; SER-505 AND THR-524, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71554 Genomic DNA. Translation: CAA96190.1.
PIR	S63252.
RefSeq	NP_014121.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6489N.
IntAct	P53836. 13 interactions.
STRING	P53836.
Genome annotation databases
Ensembl	YNL278W; YNL278W; YNL278W; Saccharomyces cerevisiae. [Genome view]
GeneID	855443.
KEGG	sce:YNL278W.
NMPDR	fig|4932.3.peg.5185.
Organism-specific databases
CYGD	YNL278w.
SGD	S000005222. CAF120.
Phylogenomic databases
HOGENOM	HBG202745.
OrthoDB	EOG9NS4TP.
Gene expression databases
ArrayExpress	P53836.
Genevestigator	P53836.
GermOnline	YNL278W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979340.

Entry information

Entry name

CA120_YEAST

Accession

Primary (citable) accession number: P53836

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 15, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)