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Protein

CCR4-NOT transcriptional complex subunit CAF120

Gene

CAF120

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcriptional complex subunit CAF120
Alternative name(s):
120 kDa CCR4-associated factor
Gene namesi
Name:CAF120
Ordered Locus Names:YNL278W
ORF Names:N0610
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL278W.
SGDiS000005222. CAF120.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10601060CCR4-NOT transcriptional complex subunit CAF120PRO_0000203378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei491 – 4911PhosphoserineCombined sources
Modified residuei510 – 5101PhosphoserineCombined sources
Modified residuei518 – 5181PhosphoserineCombined sources
Modified residuei538 – 5381PhosphoserineCombined sources
Modified residuei556 – 5561PhosphoserineCombined sources
Modified residuei871 – 8711PhosphoserineCombined sources
Modified residuei885 – 8851PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53836.

PTM databases

iPTMnetiP53836.

Interactioni

Subunit structurei

Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, CAF120, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.1 Publication

Protein-protein interaction databases

BioGridi35563. 34 interactions.
DIPiDIP-6489N.
IntActiP53836. 2 interactions.
MINTiMINT-4500708.

Structurei

3D structure databases

ProteinModelPortaliP53836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 204130PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi886 – 1030145Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the CAF120 family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000068670.
HOGENOMiHOG000057106.
InParanoidiP53836.
KOiK12609.
OrthoDBiEOG7HF1V1.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIFSGDNKV VDSLASNPGL MSPSNFGGDF GSRLKVNVTS KKKLNDSSPT
60 70 80 90 100
SPMESSPVSP ELVPILTLLN AHTHRRYHEG VFLILQDLNN NGTHAARKWK
110 120 130 140 150
DVYGVLLGTQ LALWDAKELA EFTDPSCPVS EKKLKEVASK PTYINLTDAT
160 170 180 190 200
LRTLDNSDNI VMECGKNLTN ALVVSTTLKN RYFLQFGNKE SFNAWNSAIR
210 220 230 240 250
LCLYECSSLQ EAYTGAFISS RGAKLGDIRI LLTNRKYDYK DWVSVRFGAG
260 270 280 290 300
MPWKRCYAVI SQSSSKKKGH FGEINLYEND KKVKKNHAMA TIVEAKALYA
310 320 330 340 350
VYPSSPKLID SSTIIKVVGS VKFEKKESAQ EKDVFIMPEK HQAVPSYDTI
360 370 380 390 400
IRFLIPAMDT FKLYGRPEKL LSSKNDPHSL LFGLPVLPHI YYLEVEDLLP
410 420 430 440 450
LTNSVSSLHW SNNEWKEHIS DILQRKIAQG YCGCNSTSNI TSPLPSPFLG
460 470 480 490 500
SADLFERADG VLSPKLSYGS KSSSNNSSKN SLPKRERVKL SSSSEQDLNN
510 520 530 540 550
SDSPSIKRKS PPLVISESPH KVHTPTDASF RTRVTEGSPY AKQRHPKPFA
560 570 580 590 600
SSVNDSPSDR AKSRTVPYNN NDRKATTPEK FERGETSCGK NVDESLEKVR
610 620 630 640 650
NMKLEIPESN FDKFMTDKNL LSVDSKCSNE KKLSVESDLS AIYEKYSNGP
660 670 680 690 700
FGHTEGLNGS SDETYLRFQR ASVHSESNYN SRKSFTPSDF SNGNEEEHAV
710 720 730 740 750
LQELNSLTQR INELGMESIN SNSDSDRING SYSQVDFGNN NDEDDMNLFD
760 770 780 790 800
PDFMAQDQLR AEERDYNKDD RTPLAKVPAA FQSTGLGITP DDDIERQYIT
810 820 830 840 850
EHRSRHEVPK RSPEKPSNPL EIGNPYAKPG TRLNTTHTHS KTDRSITPQR
860 870 880 890 900
GQPVPSGQQI SSYVQPANIN SPNKMYGANN SAMGSPRNPK TRAPPGPYNQ
910 920 930 940 950
GWNNRPSPSN IYQRPHPSDT QPQAYHLPGN PYSTGNRPNM QAQYHPQQVP
960 970 980 990 1000
MPILQQPNRP YQPYAMNTHM GSPGGYAGAA PPFQPANVNY NTRPQQPWPT
1010 1020 1030 1040 1050
PNSPSAHYRP PPNLNQPQNG SAGYYRPPAP QLQNSQARPQ KKDGFSQFMP
1060
SATTKNPYAQ
Length:1,060
Mass (Da):118,280
Last modified:October 1, 1996 - v1
Checksum:iCFB3F2D0B268E15B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71554 Genomic DNA. Translation: CAA96190.1.
BK006947 Genomic DNA. Translation: DAA10282.1.
PIRiS63252.
RefSeqiNP_014121.1. NM_001183116.1.

Genome annotation databases

EnsemblFungiiYNL278W; YNL278W; YNL278W.
GeneIDi855443.
KEGGisce:YNL278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71554 Genomic DNA. Translation: CAA96190.1.
BK006947 Genomic DNA. Translation: DAA10282.1.
PIRiS63252.
RefSeqiNP_014121.1. NM_001183116.1.

3D structure databases

ProteinModelPortaliP53836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35563. 34 interactions.
DIPiDIP-6489N.
IntActiP53836. 2 interactions.
MINTiMINT-4500708.

PTM databases

iPTMnetiP53836.

Proteomic databases

MaxQBiP53836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL278W; YNL278W; YNL278W.
GeneIDi855443.
KEGGisce:YNL278W.

Organism-specific databases

EuPathDBiFungiDB:YNL278W.
SGDiS000005222. CAF120.

Phylogenomic databases

GeneTreeiENSGT00530000068670.
HOGENOMiHOG000057106.
InParanoidiP53836.
KOiK12609.
OrthoDBiEOG7HF1V1.

Enzyme and pathway databases

BioCyciYEAST:G3O-33270-MONOMER.

Miscellaneous databases

PROiP53836.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
    Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
    J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510; SER-518; SER-538 AND SER-556, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491; SER-556; SER-871 AND SER-885, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCA120_YEAST
AccessioniPrimary (citable) accession number: P53836
Secondary accession number(s): D6W0R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.