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P53835

- PRM1_YEAST

UniProt

P53835 - PRM1_YEAST

Protein

Plasma membrane fusion protein PRM1

Gene

PRM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.5 Publications

    GO - Biological processi

    1. plasma membrane fusion involved in cytogamy Source: SGD

    Keywords - Biological processi

    Conjugation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33271-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasma membrane fusion protein PRM1
    Alternative name(s):
    Pheromone-regulated membrane protein 1
    Gene namesi
    Name:PRM1
    Ordered Locus Names:YNL279W
    ORF Names:N0605
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL279w.
    SGDiS000005223. PRM1.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
    Note: Localizes at sites of cell fusion during mating.

    GO - Cellular componenti

    1. integral component of membrane Source: SGD
    2. mating projection tip Source: SGD
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 661661Plasma membrane fusion protein PRM1PRO_0000203377Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi512 – 5121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi531 – 5311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi573 – 5731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi587 – 5871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP53835.
    PeptideAtlasiP53835.

    Expressioni

    Inductioni

    By pheromones during mating, through the regulation by the STE12 transcription factor. Also induced in respiratory-deficient cells.3 Publications

    Gene expression databases

    GenevestigatoriP53835.

    Interactioni

    Protein-protein interaction databases

    BioGridi35562. 17 interactions.
    DIPiDIP-1385N.
    IntActiP53835. 3 interactions.
    MINTiMINT-384905.
    STRINGi4932.YNL279W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53835.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini38 – 10568CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini127 – 296170ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini318 – 424107CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini446 – 629184ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini651 – 66111CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei106 – 12621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei297 – 31721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei425 – 44521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei630 – 65021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PRM1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG74887.
    HOGENOMiHOG000115701.
    OMAiLTWVHDH.
    OrthoDBiEOG715QCZ.

    Family and domain databases

    InterProiIPR026777. PRM1.
    [Graphical view]
    PANTHERiPTHR31030. PTHR31030. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53835-1 [UniParc]FASTAAdd to Basket

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    MSGFKCYLQL GDRLSQIWLN KYTLVLLLAM LKLLFFSKSI QHAIEVSETY    50
    ILSNCYSIDS LYSKMTDNTP HYLGIMGNYL IEKGMEETVK ATLETLSLIV 100
    YASEGLVNFA IDLYLGTYAC LIVSAVDGTV DVATNITEKL ISLVNDTVSS 150
    VANELDTGLN DISKIINKVI KAASKVENFF TGDDDDSNMT SSIKSVNLTI 200
    SALHNLYIPS SINDKLEELS AKTPDFAQVK NTTKNLISVP FNEVRKNIKA 250
    VNASNIIGDT SVLYVPPVSL DNSTGICSSN QSEILAFYSI LGHVLKIATV 300
    VCITVLICFA VGAMAPVAWN EIKLWRRLCG MRDHYMLSRQ DSYTSFSSEN 350
    THELKDPFRD PPIQNGQYDV IASYQQCFQT WNTRIAGWMT NLVTFGKSPE 400
    NIDPKTKQKI EWVVAYMTSE RALCVLGIGL LGILVCICQF VMIALLKHKI 450
    SHSLTSNDGD GVQNLLKSST AVDIENQMSL WSVQTNKYIN TTETNINQEV 500
    FGWINTTTLS VNNTVATMIS DIDTTLADVF NGTLLYNPMK TVVGCAIENK 550
    LYTIEKAMTW IHDKAQLHIP RINGTQIKQA LAKQTDNSTI PTASSTSAAT 600
    ENLLENLVND MREGLLKILR AYHRITLGEL TVALVILAVW LVQLPIALVI 650
    LRLRLRKATF D 661
    Length:661
    Mass (Da):73,353
    Last modified:October 1, 1996 - v1
    Checksum:i2038CACDCEDD927D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71555 Genomic DNA. Translation: CAA96191.1.
    BK006947 Genomic DNA. Translation: DAA10281.1.
    PIRiS63253.
    RefSeqiNP_014120.1. NM_001183117.1.

    Genome annotation databases

    EnsemblFungiiYNL279W; YNL279W; YNL279W.
    GeneIDi855442.
    KEGGisce:YNL279W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71555 Genomic DNA. Translation: CAA96191.1 .
    BK006947 Genomic DNA. Translation: DAA10281.1 .
    PIRi S63253.
    RefSeqi NP_014120.1. NM_001183117.1.

    3D structure databases

    ProteinModelPortali P53835.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35562. 17 interactions.
    DIPi DIP-1385N.
    IntActi P53835. 3 interactions.
    MINTi MINT-384905.
    STRINGi 4932.YNL279W.

    Proteomic databases

    PaxDbi P53835.
    PeptideAtlasi P53835.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL279W ; YNL279W ; YNL279W .
    GeneIDi 855442.
    KEGGi sce:YNL279W.

    Organism-specific databases

    CYGDi YNL279w.
    SGDi S000005223. PRM1.

    Phylogenomic databases

    eggNOGi NOG74887.
    HOGENOMi HOG000115701.
    OMAi LTWVHDH.
    OrthoDBi EOG715QCZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33271-MONOMER.

    Miscellaneous databases

    NextBioi 979337.

    Gene expression databases

    Genevestigatori P53835.

    Family and domain databases

    InterProi IPR026777. PRM1.
    [Graphical view ]
    PANTHERi PTHR31030. PTHR31030. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating."
      Heiman M.G., Walter P.
      J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INDUCTION.
    4. Cited for: INDUCTION.
    5. Cited for: SUBCELLULAR LOCATION.
    6. "Program-specific distribution of a transcription factor dependent on partner transcription factor and MAPK signaling."
      Zeitlinger J., Simon I., Harbison C.T., Hannett N.M., Volkert T.L., Fink G.R., Young R.A.
      Cell 113:395-404(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Prm1 prevents contact-dependent lysis of yeast mating pairs."
      Jin H., Carlile C., Nolan S., Grote E.
      Eukaryot. Cell 3:1664-1673(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating."
      Heiman M.G., Engel A., Walter P.
      J. Cell Biol. 176:209-222(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating."
      Aguilar P.S., Engel A., Walter P.
      Mol. Biol. Cell 18:547-556(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast."
      Jin H., McCaffery J.M., Grote E.
      J. Cell Biol. 180:813-826(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPRM1_YEAST
    AccessioniPrimary (citable) accession number: P53835
    Secondary accession number(s): D6W0R5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3