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P53835

- PRM1_YEAST

UniProt

P53835 - PRM1_YEAST

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Protein

Plasma membrane fusion protein PRM1

Gene
PRM1, YNL279W, N0605
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.5 Publications

GO - Biological processi

  1. plasma membrane fusion involved in cytogamy Source: SGD
Complete GO annotation...

Keywords - Biological processi

Conjugation

Enzyme and pathway databases

BioCyciYEAST:G3O-33271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane fusion protein PRM1
Alternative name(s):
Pheromone-regulated membrane protein 1
Gene namesi
Name:PRM1
Ordered Locus Names:YNL279W
ORF Names:N0605
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL279w.
SGDiS000005223. PRM1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein
Note: Localizes at sites of cell fusion during mating.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616Extracellular Reviewed predictionAdd
BLAST
Transmembranei17 – 3721Helical; Reviewed predictionAdd
BLAST
Topological domaini38 – 10568Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei106 – 12621Helical; Reviewed predictionAdd
BLAST
Topological domaini127 – 296170Extracellular Reviewed predictionAdd
BLAST
Transmembranei297 – 31721Helical; Reviewed predictionAdd
BLAST
Topological domaini318 – 424107Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei425 – 44521Helical; Reviewed predictionAdd
BLAST
Topological domaini446 – 629184Extracellular Reviewed predictionAdd
BLAST
Transmembranei630 – 65021Helical; Reviewed predictionAdd
BLAST
Topological domaini651 – 66111Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: SGD
  2. mating projection tip Source: SGD
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661Plasma membrane fusion protein PRM1PRO_0000203377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi188 – 1881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi197 – 1971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi231 – 2311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi252 – 2521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi272 – 2721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi280 – 2801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi490 – 4901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi505 – 5051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi512 – 5121N-linked (GlcNAc...) Reviewed prediction
Glycosylationi531 – 5311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi573 – 5731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi587 – 5871N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP53835.
PeptideAtlasiP53835.

Expressioni

Inductioni

By pheromones during mating, through the regulation by the STE12 transcription factor. Also induced in respiratory-deficient cells.3 Publications

Gene expression databases

GenevestigatoriP53835.

Interactioni

Protein-protein interaction databases

BioGridi35562. 17 interactions.
DIPiDIP-1385N.
IntActiP53835. 3 interactions.
MINTiMINT-384905.
STRINGi4932.YNL279W.

Structurei

3D structure databases

ProteinModelPortaliP53835.

Family & Domainsi

Sequence similaritiesi

Belongs to the PRM1 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG74887.
HOGENOMiHOG000115701.
OMAiLTWVHDH.
OrthoDBiEOG715QCZ.

Family and domain databases

InterProiIPR026777. PRM1.
[Graphical view]
PANTHERiPTHR31030. PTHR31030. 1 hit.

Sequencei

Sequence statusi: Complete.

P53835-1 [UniParc]FASTAAdd to Basket

« Hide

MSGFKCYLQL GDRLSQIWLN KYTLVLLLAM LKLLFFSKSI QHAIEVSETY    50
ILSNCYSIDS LYSKMTDNTP HYLGIMGNYL IEKGMEETVK ATLETLSLIV 100
YASEGLVNFA IDLYLGTYAC LIVSAVDGTV DVATNITEKL ISLVNDTVSS 150
VANELDTGLN DISKIINKVI KAASKVENFF TGDDDDSNMT SSIKSVNLTI 200
SALHNLYIPS SINDKLEELS AKTPDFAQVK NTTKNLISVP FNEVRKNIKA 250
VNASNIIGDT SVLYVPPVSL DNSTGICSSN QSEILAFYSI LGHVLKIATV 300
VCITVLICFA VGAMAPVAWN EIKLWRRLCG MRDHYMLSRQ DSYTSFSSEN 350
THELKDPFRD PPIQNGQYDV IASYQQCFQT WNTRIAGWMT NLVTFGKSPE 400
NIDPKTKQKI EWVVAYMTSE RALCVLGIGL LGILVCICQF VMIALLKHKI 450
SHSLTSNDGD GVQNLLKSST AVDIENQMSL WSVQTNKYIN TTETNINQEV 500
FGWINTTTLS VNNTVATMIS DIDTTLADVF NGTLLYNPMK TVVGCAIENK 550
LYTIEKAMTW IHDKAQLHIP RINGTQIKQA LAKQTDNSTI PTASSTSAAT 600
ENLLENLVND MREGLLKILR AYHRITLGEL TVALVILAVW LVQLPIALVI 650
LRLRLRKATF D 661
Length:661
Mass (Da):73,353
Last modified:October 1, 1996 - v1
Checksum:i2038CACDCEDD927D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71555 Genomic DNA. Translation: CAA96191.1.
BK006947 Genomic DNA. Translation: DAA10281.1.
PIRiS63253.
RefSeqiNP_014120.1. NM_001183117.1.

Genome annotation databases

EnsemblFungiiYNL279W; YNL279W; YNL279W.
GeneIDi855442.
KEGGisce:YNL279W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71555 Genomic DNA. Translation: CAA96191.1 .
BK006947 Genomic DNA. Translation: DAA10281.1 .
PIRi S63253.
RefSeqi NP_014120.1. NM_001183117.1.

3D structure databases

ProteinModelPortali P53835.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35562. 17 interactions.
DIPi DIP-1385N.
IntActi P53835. 3 interactions.
MINTi MINT-384905.
STRINGi 4932.YNL279W.

Proteomic databases

PaxDbi P53835.
PeptideAtlasi P53835.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL279W ; YNL279W ; YNL279W .
GeneIDi 855442.
KEGGi sce:YNL279W.

Organism-specific databases

CYGDi YNL279w.
SGDi S000005223. PRM1.

Phylogenomic databases

eggNOGi NOG74887.
HOGENOMi HOG000115701.
OMAi LTWVHDH.
OrthoDBi EOG715QCZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-33271-MONOMER.

Miscellaneous databases

NextBioi 979337.

Gene expression databases

Genevestigatori P53835.

Family and domain databases

InterProi IPR026777. PRM1.
[Graphical view ]
PANTHERi PTHR31030. PTHR31030. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating."
    Heiman M.G., Walter P.
    J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INDUCTION.
  4. Cited for: INDUCTION.
  5. Cited for: SUBCELLULAR LOCATION.
  6. "Program-specific distribution of a transcription factor dependent on partner transcription factor and MAPK signaling."
    Zeitlinger J., Simon I., Harbison C.T., Hannett N.M., Volkert T.L., Fink G.R., Young R.A.
    Cell 113:395-404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Prm1 prevents contact-dependent lysis of yeast mating pairs."
    Jin H., Carlile C., Nolan S., Grote E.
    Eukaryot. Cell 3:1664-1673(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating."
    Heiman M.G., Engel A., Walter P.
    J. Cell Biol. 176:209-222(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating."
    Aguilar P.S., Engel A., Walter P.
    Mol. Biol. Cell 18:547-556(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast."
    Jin H., McCaffery J.M., Grote E.
    J. Cell Biol. 180:813-826(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPRM1_YEAST
AccessioniPrimary (citable) accession number: P53835
Secondary accession number(s): D6W0R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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