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P53835

- PRM1_YEAST

UniProt

P53835 - PRM1_YEAST

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Protein

Plasma membrane fusion protein PRM1

Gene

PRM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.5 Publications

GO - Biological processi

  1. plasma membrane fusion involved in cytogamy Source: SGD
Complete GO annotation...

Keywords - Biological processi

Conjugation

Enzyme and pathway databases

BioCyciYEAST:G3O-33271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane fusion protein PRM1
Alternative name(s):
Pheromone-regulated membrane protein 1
Gene namesi
Name:PRM1
Ordered Locus Names:YNL279W
ORF Names:N0605
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL279w.
SGDiS000005223. PRM1.

Subcellular locationi

Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
Note: Localizes at sites of cell fusion during mating.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616ExtracellularSequence AnalysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence AnalysisAdd
BLAST
Topological domaini38 – 10568CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei106 – 12621HelicalSequence AnalysisAdd
BLAST
Topological domaini127 – 296170ExtracellularSequence AnalysisAdd
BLAST
Transmembranei297 – 31721HelicalSequence AnalysisAdd
BLAST
Topological domaini318 – 424107CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei425 – 44521HelicalSequence AnalysisAdd
BLAST
Topological domaini446 – 629184ExtracellularSequence AnalysisAdd
BLAST
Transmembranei630 – 65021HelicalSequence AnalysisAdd
BLAST
Topological domaini651 – 66111CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: SGD
  2. mating projection tip Source: SGD
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661Plasma membrane fusion protein PRM1PRO_0000203377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi512 – 5121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi531 – 5311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi573 – 5731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi587 – 5871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP53835.
PeptideAtlasiP53835.

Expressioni

Inductioni

By pheromones during mating, through the regulation by the STE12 transcription factor. Also induced in respiratory-deficient cells.3 Publications

Gene expression databases

GenevestigatoriP53835.

Interactioni

Protein-protein interaction databases

BioGridi35562. 17 interactions.
DIPiDIP-1385N.
IntActiP53835. 3 interactions.
MINTiMINT-384905.
STRINGi4932.YNL279W.

Structurei

3D structure databases

ProteinModelPortaliP53835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PRM1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG74887.
HOGENOMiHOG000115701.
InParanoidiP53835.
OMAiLTWVHDH.
OrthoDBiEOG715QCZ.

Family and domain databases

InterProiIPR026777. PRM1.
[Graphical view]
PANTHERiPTHR31030. PTHR31030. 1 hit.

Sequencei

Sequence statusi: Complete.

P53835-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGFKCYLQL GDRLSQIWLN KYTLVLLLAM LKLLFFSKSI QHAIEVSETY
60 70 80 90 100
ILSNCYSIDS LYSKMTDNTP HYLGIMGNYL IEKGMEETVK ATLETLSLIV
110 120 130 140 150
YASEGLVNFA IDLYLGTYAC LIVSAVDGTV DVATNITEKL ISLVNDTVSS
160 170 180 190 200
VANELDTGLN DISKIINKVI KAASKVENFF TGDDDDSNMT SSIKSVNLTI
210 220 230 240 250
SALHNLYIPS SINDKLEELS AKTPDFAQVK NTTKNLISVP FNEVRKNIKA
260 270 280 290 300
VNASNIIGDT SVLYVPPVSL DNSTGICSSN QSEILAFYSI LGHVLKIATV
310 320 330 340 350
VCITVLICFA VGAMAPVAWN EIKLWRRLCG MRDHYMLSRQ DSYTSFSSEN
360 370 380 390 400
THELKDPFRD PPIQNGQYDV IASYQQCFQT WNTRIAGWMT NLVTFGKSPE
410 420 430 440 450
NIDPKTKQKI EWVVAYMTSE RALCVLGIGL LGILVCICQF VMIALLKHKI
460 470 480 490 500
SHSLTSNDGD GVQNLLKSST AVDIENQMSL WSVQTNKYIN TTETNINQEV
510 520 530 540 550
FGWINTTTLS VNNTVATMIS DIDTTLADVF NGTLLYNPMK TVVGCAIENK
560 570 580 590 600
LYTIEKAMTW IHDKAQLHIP RINGTQIKQA LAKQTDNSTI PTASSTSAAT
610 620 630 640 650
ENLLENLVND MREGLLKILR AYHRITLGEL TVALVILAVW LVQLPIALVI
660
LRLRLRKATF D
Length:661
Mass (Da):73,353
Last modified:October 1, 1996 - v1
Checksum:i2038CACDCEDD927D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71555 Genomic DNA. Translation: CAA96191.1.
BK006947 Genomic DNA. Translation: DAA10281.1.
PIRiS63253.
RefSeqiNP_014120.1. NM_001183117.1.

Genome annotation databases

EnsemblFungiiYNL279W; YNL279W; YNL279W.
GeneIDi855442.
KEGGisce:YNL279W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71555 Genomic DNA. Translation: CAA96191.1 .
BK006947 Genomic DNA. Translation: DAA10281.1 .
PIRi S63253.
RefSeqi NP_014120.1. NM_001183117.1.

3D structure databases

ProteinModelPortali P53835.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35562. 17 interactions.
DIPi DIP-1385N.
IntActi P53835. 3 interactions.
MINTi MINT-384905.
STRINGi 4932.YNL279W.

Proteomic databases

PaxDbi P53835.
PeptideAtlasi P53835.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL279W ; YNL279W ; YNL279W .
GeneIDi 855442.
KEGGi sce:YNL279W.

Organism-specific databases

CYGDi YNL279w.
SGDi S000005223. PRM1.

Phylogenomic databases

eggNOGi NOG74887.
HOGENOMi HOG000115701.
InParanoidi P53835.
OMAi LTWVHDH.
OrthoDBi EOG715QCZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-33271-MONOMER.

Miscellaneous databases

NextBioi 979337.

Gene expression databases

Genevestigatori P53835.

Family and domain databases

InterProi IPR026777. PRM1.
[Graphical view ]
PANTHERi PTHR31030. PTHR31030. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating."
    Heiman M.G., Walter P.
    J. Cell Biol. 151:719-730(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INDUCTION.
  4. Cited for: INDUCTION.
  5. Cited for: SUBCELLULAR LOCATION.
  6. "Program-specific distribution of a transcription factor dependent on partner transcription factor and MAPK signaling."
    Zeitlinger J., Simon I., Harbison C.T., Hannett N.M., Volkert T.L., Fink G.R., Young R.A.
    Cell 113:395-404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Prm1 prevents contact-dependent lysis of yeast mating pairs."
    Jin H., Carlile C., Nolan S., Grote E.
    Eukaryot. Cell 3:1664-1673(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating."
    Heiman M.G., Engel A., Walter P.
    J. Cell Biol. 176:209-222(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating."
    Aguilar P.S., Engel A., Walter P.
    Mol. Biol. Cell 18:547-556(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast."
    Jin H., McCaffery J.M., Grote E.
    J. Cell Biol. 180:813-826(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPRM1_YEAST
AccessioniPrimary (citable) accession number: P53835
Secondary accession number(s): D6W0R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3