P53833 (POP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleases P/MRP protein subunit POP3

Alternative name(s):
RNA-processing protein POP3
RNases MRP/P 22.6 kDa subunit

Gene names

Name:	POP3
Ordered Locus Names:	YNL282W
ORF Names:	N0586

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for processing of 5.8S rRNA (short form) at site A3 and for 5'- and 3'-processing of pre-tRNA. Ref.1 Ref.4
Subunit structure	Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex. Ref.1
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 1360 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the POP3 family.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intronic box C/D snoRNA processing Inferred from direct assay. Source: SGD mRNA cleavage Inferred from physical interaction. Source: SGD rRNA processing Inferred from direct assay Ref.4. Source: SGD tRNA processing Inferred from direct assay Ref.4. Source: SGD
Cellular component	nucleolar ribonuclease P complex Inferred from direct assay Ref.4. Source: SGD ribonuclease MRP complex Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Ribonucleases P/MRP protein subunit POP3		PRO_0000058516

Sequences

	Sequence		Length	Mass (Da)
	P53833 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5091EE590BDBCBEA	FASTA	195	22,612
	10 20 30 40 50 60 MSGSLKSLDK KIAKRRQVYK PVLDNPFTNE AHMWPRVHDQ PLIWQLLQSS IINKLIHIQS 70 80 90 100 110 120 KENYPWELYT DFNEIVQYLS GAHGNSDPVC LFVCNKDPDV PLVLLQQIPL LCYMAPMTVK 130 140 150 160 170 180 LVQLPKSAMD TFKSVSKYGM LLLRCDDRVD KKFVSQIQKN VDLLQFPWLN AIKYRPTSVK 190 LLKTTVPIVS KKRQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pop3p is essential for the activity of the RNase MRP and RNase P ribonucleoproteins in vivo." Dichtl B., Tollervey D. EMBO J. 16:417-429(1997) [PubMed: 9029160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: SL311.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP." Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R. Genes Dev. 12:1678-1690(1998) [PubMed: 9620854] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component." Salinas K., Wierzbicki S., Zhou L., Schmitt M.E. J. Biol. Chem. 280:11352-11360(2005) [PubMed: 15637077] [Abstract] Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95844 Genomic DNA. Translation: CAA65102.1. Z71558 Genomic DNA. Translation: CAA96194.1. BK006947 Genomic DNA. Translation: DAA10278.1.
PIR	S63256.
RefSeq	NP_014117.1. NM_001183120.1.
3D structure databases
ProteinModelPortal	P53833.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4997N.
IntAct	P53833. 10 interactions.
MINT	MINT-515744.
STRING	P53833.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL282W; YNL282W; YNL282W.
GeneID	855439.
KEGG	sce:YNL282W.
NMPDR	fig|4932.3.peg.5181.
Organism-specific databases
CYGD	YNL282w.
SGD	S000005226. POP3.
Phylogenomic databases
eggNOG	fuNOG11791.
HOGENOM	HBG202743.
OMA	AKRRQVY.
OrthoDB	EOG4H75N2.
Gene expression databases
ArrayExpress	P53833.
Genevestigator	P53833.
GermOnline	YNL282W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013241. RNase_P_Pop3. [Graphical view]
KO	K14522.
Pfam	PF08228. RNase_P_pop3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979328.

Entry information

Entry name

POP3_YEAST

Accession

Primary (citable) accession number: P53833
Secondary accession number(s): D6W0R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program