Reviewed, UniProtKB/Swiss-Prot P53830 (YN26_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized RNA-binding protein YNL286W

Gene names

Ordered Locus Names:	YNL286W
ORF Names:	N0549

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 1360 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from genetic interaction. Source: SGD
Cellular component	U2 snRNP Traceable author statement. Source: SGD
Molecular function	RNA binding Inferred from mutant phenotype. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Uncharacterized RNA-binding protein YNL286W		PRO_0000082037
Regions
	Domain	45 – 130	86	RRM
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53830-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 69A926BF0DB2C6FE	FASTA	285	32,284
	10 20 30 40 50 60 MDADELELKG HLKKLKKEEL LRRKQLKESN LQKRELEYNN ASKNTSIYIS GLPTDKTTKE 70 80 90 100 110 120 GLTEQFCKYG MIRTNRDGEP LCKLYVNDKG AFKGDALITY SKEESVTLAI EMMNESIFLG 130 140 150 160 170 180 KQIRVERAQF QNKEGDNMHG KENDLKEFNG PEPPIKRLKK AKSEGEGEVI DYNDDESLAK 190 200 210 220 230 240 ADRTVIFANV FNIYKSYTND DINDIQEDLL EGCEEIGQVD SISVSPNKGE ATVVFKNNKV 250 260 270 280 ALQCCKIMTG RYFDGQKLLA FISGDENTSS TSDKNEDSEV EDDLI

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71562 Genomic DNA. Translation: CAA96203.1.
PIR	S63260.
RefSeq	NP_014113.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2316N.
IntAct	P53830. 5 interactions.
STRING	P53830.
Genome annotation databases
Ensembl	YNL286W; YNL286W; YNL286W; Saccharomyces cerevisiae. [Genome view]
GeneID	855430.
KEGG	sce:YNL286W.
NMPDR	fig|4932.3.peg.5175.
Organism-specific databases
CYGD	YNL286w.
SGD	S000005230. CUS2.
Phylogenomic databases
OMA	IEMMDNT.
OrthoDB	EOG94N084.
Gene expression databases
ArrayExpress	P53830.
Genevestigator	P53830.
GermOnline	YNL286W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979307.

Entry information

Entry name

YN26_YEAST

Accession

Primary (citable) accession number: P53830

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 15, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)