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Protein

Cold sensitive U2 snRNA suppressor 2

Gene

CUS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

U2 snRNP protein which helps to refold U2 into a structure favorable for its binding to SF3b and SF3a prior to spliceosome assembly. Mediates functional interactions between U2 RNA and PRP5. Enforces ATP dependence during formation of the prespliceosome by brokering an interaction between PRP5 and the U2 snRNP that depends on correct U2 RNA structure.3 Publications

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • U2 snRNA binding Source: GO_Central

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • RNA folding Source: SGD
  • U2-type prespliceosome assembly Source: GO_Central

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-33276-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cold sensitive U2 snRNA suppressor 2
Gene namesi
Name:CUS2
Ordered Locus Names:YNL286W
ORF Names:N0549
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL286W
SGDiS000005230 CUS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000820371 – 285Cold sensitive U2 snRNA suppressor 2Add BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei163PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53830
PaxDbiP53830
PRIDEiP53830

PTM databases

iPTMnetiP53830

Interactioni

Subunit structurei

Interacts with PRP11. Associates with the U2 snRNA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSH155P499553EBI-28299,EBI-664

Protein-protein interaction databases

BioGridi35551, 46 interactors
DIPiDIP-2316N
IntActiP53830, 8 interactors
MINTiP53830
STRINGi4932.YNL286W

Structurei

3D structure databases

ProteinModelPortaliP53830
SMRiP53830
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 130RRM 1PROSITE-ProRule annotationAdd BLAST86
Domaini183 – 265RRM 2PROSITE-ProRule annotationAdd BLAST83

Sequence similaritiesi

Belongs to the HTATSF1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000009902
InParanoidiP53830
KOiK13093
OMAiANDIHIK
OrthoDBiEOG092C5OTL

Family and domain databases

CDDicd12281 RRM1_TatSF1_like, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034393 TatSF1-like
IPR034392 TatSF1-like_RRM1
PANTHERiPTHR15608 PTHR15608, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

P53830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDADELELKG HLKKLKKEEL LRRKQLKESN LQKRELEYNN ASKNTSIYIS
60 70 80 90 100
GLPTDKTTKE GLTEQFCKYG MIRTNRDGEP LCKLYVNDKG AFKGDALITY
110 120 130 140 150
SKEESVTLAI EMMNESIFLG KQIRVERAQF QNKEGDNMHG KENDLKEFNG
160 170 180 190 200
PEPPIKRLKK AKSEGEGEVI DYNDDESLAK ADRTVIFANV FNIYKSYTND
210 220 230 240 250
DINDIQEDLL EGCEEIGQVD SISVSPNKGE ATVVFKNNKV ALQCCKIMTG
260 270 280
RYFDGQKLLA FISGDENTSS TSDKNEDSEV EDDLI
Length:285
Mass (Da):32,284
Last modified:October 1, 1996 - v1
Checksum:i69A926BF0DB2C6FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235F → L in AAS56342 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71562 Genomic DNA Translation: CAA96203.1
AY558016 Genomic DNA Translation: AAS56342.1
BK006947 Genomic DNA Translation: DAA10273.1
PIRiS63260
RefSeqiNP_014113.1, NM_001183124.1

Genome annotation databases

EnsemblFungiiYNL286W; YNL286W; YNL286W
GeneIDi855430
KEGGisce:YNL286W

Similar proteinsi

Entry informationi

Entry nameiCUS2_YEAST
AccessioniPrimary (citable) accession number: P53830
Secondary accession number(s): D6W0Q7, E9P8U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health