P53830 (CUS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cold sensitive U2 snRNA suppressor 2

Gene names

Name:	CUS2
Ordered Locus Names:	YNL286W
ORF Names:	N0549

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	U2 snRNP protein which helps to refold U2 into a structure favorable for its binding to SF3b and SF3a prior to spliceosome assembly. Mediates functional interactions between U2 RNA and PRP5. Enforces ATP dependence during formation of the prespliceosome by brokering an interaction between PRP5 and the U2 snRNP that depends on correct U2 RNA structure. Ref.4 Ref.5 Ref.9
Subunit structure	Interacts with PRP11. Associates with the U2 snRNA. Ref.4
Miscellaneous	Present with 1360 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the HTATSF1 family. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Spliceosome
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from genetic interaction. Source: SGD
Cellular component	U2 snRNP Traceable author statement Ref.6. Source: SGD spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from mutant phenotype Ref.4. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Cold sensitive U2 snRNA suppressor 2		PRO_0000082037
Regions
	Domain	45 – 130	86	RRM 1
	Domain	183 – 265	83	RRM 2
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	235	1	F → L in AAS56342. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53830 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 69A926BF0DB2C6FE	FASTA	285	32,284
	10 20 30 40 50 60 MDADELELKG HLKKLKKEEL LRRKQLKESN LQKRELEYNN ASKNTSIYIS GLPTDKTTKE 70 80 90 100 110 120 GLTEQFCKYG MIRTNRDGEP LCKLYVNDKG AFKGDALITY SKEESVTLAI EMMNESIFLG 130 140 150 160 170 180 KQIRVERAQF QNKEGDNMHG KENDLKEFNG PEPPIKRLKK AKSEGEGEVI DYNDDESLAK 190 200 210 220 230 240 ADRTVIFANV FNIYKSYTND DINDIQEDLL EGCEEIGQVD SISVSPNKGE ATVVFKNNKV 250 260 270 280 ALQCCKIMTG RYFDGQKLLA FISGDENTSS TSDKNEDSEV EDDLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif." Yan D., Perriman R., Igel H., Howe K.J., Neville M., Ares M. Jr. Mol. Cell. Biol. 18:5000-5009(1998) [PubMed: 9710584] [Abstract] Cited for: FUNCTION, INTERACTION WITH PRP11, ASSOCIATION WITH U2.
[5]	"ATP can be dispensable for prespliceosome formation in yeast." Perriman R., Ares M. Jr. Genes Dev. 14:97-107(2000) [PubMed: 10640279] [Abstract] Cited for: FUNCTION.
[6]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract] Cited for: IDENTIFICATION IN THE U2 SNRNP, MASS SPECTROMETRY.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Competition between the ATPase Prp5 and branch region-U2 snRNA pairing modulates the fidelity of spliceosome assembly." Xu Y.Z., Query C.C. Mol. Cell 28:838-849(2007) [PubMed: 18082608] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71562 Genomic DNA. Translation: CAA96203.1. AY558016 Genomic DNA. Translation: AAS56342.1. BK006947 Genomic DNA. Translation: DAA10273.1.
PIR	S63260.
RefSeq	NP_014113.1. NM_001183124.1.
3D structure databases
ProteinModelPortal	P53830.
SMR	P53830. Positions 39-129, 174-273.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2316N.
IntAct	P53830. 5 interactions.
MINT	MINT-2787174.
STRING	P53830.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL286W; YNL286W; YNL286W.
GeneID	855430.
KEGG	sce:YNL286W.
NMPDR	fig|4932.3.peg.5175.
Organism-specific databases
CYGD	YNL286w.
SGD	S000005230. CUS2.
Phylogenomic databases
eggNOG	fuNOG06041.
OMA	IEMMDNT.
OrthoDB	EOG4JWZQ8.
Gene expression databases
ArrayExpress	P53830.
Genevestigator	P53830.
GermOnline	YNL286W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
KO	K13093.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979307.

Entry information

Entry name

CUS2_YEAST

Accession

Primary (citable) accession number: P53830
Secondary accession number(s): D6W0Q7, E9P8U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program