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Protein

Probable pyridoxal 5'-phosphate synthase subunit SNZ2

Gene

SNZ2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by a SNO isoform. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.By similarity

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211D-ribose 5-phosphateBy similarity
Active sitei78 – 781Schiff-base intermediate with D-ribose 5-phosphateBy similarity
Binding sitei150 – 1501D-ribose 5-phosphate; via amide nitrogenBy similarity
Binding sitei213 – 2131D-ribose 5-phosphate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. GMP synthase (glutamine-hydrolyzing) activity Source: SGD
  2. pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity Source: UniProtKB-EC

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: InterPro
  2. pyridoxine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate, Schiff base

Enzyme and pathway databases

BioCyciYEAST:G3O-33316-MONOMER.
UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyridoxal 5'-phosphate synthase subunit SNZ2 (EC:4.3.3.6)
Short name:
PLP synthase subunit SNZ2
Alternative name(s):
PDX1 homolog 2
Short name:
Pdx1.2
Gene namesi
Name:SNZ2
Ordered Locus Names:YNL333W
ORF Names:N0290
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL333w.
SGDiS000005277. SNZ2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable pyridoxal 5'-phosphate synthase subunit SNZ2PRO_0000109359Add
BLAST

Proteomic databases

MaxQBiP53824.
PaxDbiP53824.

Expressioni

Inductioni

By the absence of external thiamine.1 Publication

Gene expression databases

GenevestigatoriP53824.

Interactioni

Subunit structurei

Homohexamer (By similarity). Interacts with THI11 (PubMed:12271461).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SNO1Q031443EBI-17623,EBI-28190

Protein-protein interaction databases

BioGridi35508. 13 interactions.
DIPiDIP-1695N.
IntActiP53824. 12 interactions.
MINTiMINT-406912.
STRINGi4932.YNL333W.

Structurei

3D structure databases

ProteinModelPortaliP53824.
SMRiP53824. Positions 14-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni234 – 2352D-ribose 5-phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the PdxS/SNZ family.Curated

Phylogenomic databases

eggNOGiCOG0214.
GeneTreeiENSGT00390000018460.
HOGENOMiHOG000227586.
InParanoidiP53824.
OMAiDEDNHID.
OrthoDBiEOG7TN031.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_01824. PdxS.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR001852. Snz1p/Sor1.
[Graphical view]
PfamiPF01680. SOR_SNZ. 1 hit.
[Graphical view]
PIRSFiPIRSF029271. Pdx1. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00343. TIGR00343. 1 hit.
PROSITEiPS01235. PDXS_SNZ_1. 1 hit.
PS51129. PDXS_SNZ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFKVKTGL AQMLKGGVIM DVVTPEQAII AERAGACAVM ALERIPADMR
60 70 80 90 100
KSGQVCRMSD PRMIKEIMEA VSIPVMAKVR IGHFVEAQIL EELQVDYIDE
110 120 130 140 150
SEVLTPADWT HHIEKHNFKV PFVCGAKDLG EALRRINEGA AMIRTKGEAG
160 170 180 190 200
TGDVSEAVKH ITKIKAEIQQ YKENLKTESD FAAKATELRV PVDLLKTTLS
210 220 230 240 250
EGKLPVVNFA AGGVATPADA ALLMQLGCEG VFVGSGIFKS SDPEKLACAI
260 270 280 290
VEATTHYDNP AKLLQISSDL GDLMGGISIQ SINEAGGKNG ARLSEIGW
Length:298
Mass (Da):32,033
Last modified:October 1, 1996 - v1
Checksum:iEE02E7C546C460AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71608 Genomic DNA. Translation: CAA96266.1.
Z71609 Genomic DNA. Translation: CAA96267.1.
AY692873 Genomic DNA. Translation: AAT92892.1.
BK006947 Genomic DNA. Translation: DAA10230.1.
RefSeqiNP_014066.1. NM_001183171.1.

Genome annotation databases

EnsemblFungiiYNL333W; YNL333W; YNL333W.
GeneIDi855383.
KEGGisce:YNL333W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71608 Genomic DNA. Translation: CAA96266.1.
Z71609 Genomic DNA. Translation: CAA96267.1.
AY692873 Genomic DNA. Translation: AAT92892.1.
BK006947 Genomic DNA. Translation: DAA10230.1.
RefSeqiNP_014066.1. NM_001183171.1.

3D structure databases

ProteinModelPortaliP53824.
SMRiP53824. Positions 14-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35508. 13 interactions.
DIPiDIP-1695N.
IntActiP53824. 12 interactions.
MINTiMINT-406912.
STRINGi4932.YNL333W.

Proteomic databases

MaxQBiP53824.
PaxDbiP53824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL333W; YNL333W; YNL333W.
GeneIDi855383.
KEGGisce:YNL333W.

Organism-specific databases

CYGDiYNL333w.
SGDiS000005277. SNZ2.

Phylogenomic databases

eggNOGiCOG0214.
GeneTreeiENSGT00390000018460.
HOGENOMiHOG000227586.
InParanoidiP53824.
OMAiDEDNHID.
OrthoDBiEOG7TN031.

Enzyme and pathway databases

UniPathwayiUPA00245.
BioCyciYEAST:G3O-33316-MONOMER.

Miscellaneous databases

NextBioi979178.

Gene expression databases

GenevestigatoriP53824.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_01824. PdxS.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR001852. Snz1p/Sor1.
[Graphical view]
PfamiPF01680. SOR_SNZ. 1 hit.
[Graphical view]
PIRSFiPIRSF029271. Pdx1. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00343. TIGR00343. 1 hit.
PROSITEiPS01235. PDXS_SNZ_1. 1 hit.
PS51129. PDXS_SNZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: PROBABLE FUNCTION, INDUCTION, INTERACTION WITH THI11.

Entry informationi

Entry nameiSNZ2_YEAST
AccessioniPrimary (citable) accession number: P53824
Secondary accession number(s): D6W0L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.