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Protein

Probable pyridoxal 5'-phosphate synthase subunit SNO2

Gene

SNO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of a SNZ isoform.By similarity1 Publication

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.By similarity
L-glutamine + H2O = L-glutamate + NH3.By similarity

Pathway:ipyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911NucleophileBy similarity
Binding sitei120 – 1201L-glutamineBy similarity
Active sitei197 – 1971Charge relay systemBy similarity
Active sitei199 – 1991Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • pyridoxal phosphate biosynthetic process Source: UniProtKB-UniPathway
  • pyridoxine metabolic process Source: SGD
  • thiamine biosynthetic process Source: SGD
  • vitamin B6 biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:G3O-33317-MONOMER.
UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyridoxal 5'-phosphate synthase subunit SNO2 (EC:4.3.3.6)
Alternative name(s):
PDX2 homolog 2
Short name:
Pdx2.2
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2)
Gene namesi
Name:SNO2
Ordered Locus Names:YNL334C
ORF Names:N0285
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIV

Organism-specific databases

CYGDiYNL334c.
EuPathDBiFungiDB:YNL334C.
SGDiS000005278. SNO2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Probable pyridoxal 5'-phosphate synthase subunit SNO2PRO_0000135620Add
BLAST

Proteomic databases

MaxQBiP53823.
PaxDbiP53823.

Interactioni

Protein-protein interaction databases

BioGridi35507. 9 interactions.
DIPiDIP-2069N.
IntActiP53823. 6 interactions.
MINTiMINT-509304.

Structurei

3D structure databases

ProteinModelPortaliP53823.
SMRiP53823. Positions 3-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 603L-glutamine bindingBy similarity
Regioni151 – 1522L-glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0311.
GeneTreeiENSGT00390000011516.
HOGENOMiHOG000039949.
InParanoidiP53823.
KOiK08681.
OMAiAGMIYIS.
OrthoDBiEOG7BW0W3.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
[Graphical view]
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVIGVLAL QGAFIEHVRH VEKCIVENRD FYEKKLSVMT VKDKNQLAQC
60 70 80 90 100
DALIIPGGES TAMSLIAERT GFYDDLYAFV HNPSKVTWGT CAGMIYISQQ
110 120 130 140 150
LSNEEKLVKT LNLLKVKVKR NAFGRQAQSS TRICDFSNFI PHCNDFPATF
160 170 180 190 200
IRAPVIEEVL DPEHVQVLYK LDGKDNGGQE LIVAAKQKNN ILATSFHPEL
210 220
AENDIRFHDW FIREFVLKNY SK
Length:222
Mass (Da):25,208
Last modified:October 1, 1996 - v1
Checksum:i6755EE1D826CAABA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71610 Genomic DNA. Translation: CAA96268.1.
BK006947 Genomic DNA. Translation: DAA10229.1.
PIRiS63320.
RefSeqiNP_014065.1. NM_001183172.1.

Genome annotation databases

EnsemblFungiiYNL334C; YNL334C; YNL334C.
GeneIDi855382.
KEGGisce:YNL334C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71610 Genomic DNA. Translation: CAA96268.1.
BK006947 Genomic DNA. Translation: DAA10229.1.
PIRiS63320.
RefSeqiNP_014065.1. NM_001183172.1.

3D structure databases

ProteinModelPortaliP53823.
SMRiP53823. Positions 3-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35507. 9 interactions.
DIPiDIP-2069N.
IntActiP53823. 6 interactions.
MINTiMINT-509304.

Proteomic databases

MaxQBiP53823.
PaxDbiP53823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL334C; YNL334C; YNL334C.
GeneIDi855382.
KEGGisce:YNL334C.

Organism-specific databases

CYGDiYNL334c.
EuPathDBiFungiDB:YNL334C.
SGDiS000005278. SNO2.

Phylogenomic databases

eggNOGiCOG0311.
GeneTreeiENSGT00390000011516.
HOGENOMiHOG000039949.
InParanoidiP53823.
KOiK08681.
OMAiAGMIYIS.
OrthoDBiEOG7BW0W3.

Enzyme and pathway databases

UniPathwayiUPA00245.
BioCyciYEAST:G3O-33317-MONOMER.

Miscellaneous databases

NextBioi979175.
PROiP53823.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
[Graphical view]
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: FUNCTION.

Entry informationi

Entry nameiSNO2_YEAST
AccessioniPrimary (citable) accession number: P53823
Secondary accession number(s): D6W0L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.