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Protein

HRAS-like suppressor 3

Gene

Pla2g16

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. N- and O-acylation activity is hardly detectable. Also has weak lysophospholipase activity (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211By similarity
Active sitei33 – 331By similarity
Active sitei111 – 1111Acyl-thioester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • negative regulation of cell cycle Source: RGD
  • phospholipid biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.32. 5301.
ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482801. Acyl chain remodelling of PS.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-1482922. Acyl chain remodelling of PI.

Names & Taxonomyi

Protein namesi
Recommended name:
HRAS-like suppressor 3 (EC:3.1.1.32, EC:3.1.1.4)
Short name:
HRSL3
Alternative name(s):
Group XVI phospholipase A1/A2
H-rev 107 protein
Gene namesi
Name:Pla2g16
Synonyms:H-rev107, Hrasls3, Hrev107
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2829. Pla2g16.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmperinuclear region 1 Publication
  • Membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 130130CytoplasmicSequence analysisAdd
BLAST
Transmembranei131 – 15323HelicalSequence analysisAdd
BLAST
Topological domaini154 – 1607LumenalSequence analysis

GO - Cellular componenti

  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160HRAS-like suppressor 3PRO_0000152487Add
BLAST

Proteomic databases

PaxDbiP53817.

Expressioni

Tissue specificityi

Specifically expressed in H-ras resistant fibroblasts.2 Publications

Inductioni

Induced during preadipocyte differentiation into adipocytes.1 Publication

Gene expression databases

ExpressionAtlasiP53817. baseline and differential.
GenevisibleiP53817. RN.

Interactioni

Subunit structurei

Interacts with PPP2R1A; this interaction might decrease PP2A activity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038045.

Family & Domainsi

Sequence similaritiesi

Belongs to the H-rev107 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IW0Y. Eukaryota.
ENOG4111TDD. LUCA.
GeneTreeiENSGT00390000013327.
HOGENOMiHOG000293227.
InParanoidiP53817.
KOiK16817.
OMAiHVNNKHD.
OrthoDBiEOG7B8S59.
PhylomeDBiP53817.
TreeFamiTF330836.

Family and domain databases

InterProiIPR007053. LRAT-like_dom.
[Graphical view]
PfamiPF04970. LRAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIPEPKPGD LIEIFRPMYS HWAIYVGDGY VIHLAPPSEI PGAGAASIMS
60 70 80 90 100
ALTDKAIVKK ELLRDVAGKD KYQVNNKHDK EYTPLPLNKI IQRAEELVGQ
110 120 130 140 150
EVLYRLTSEN CEHFVNELRY GVPRSDQVRD AVKVATVTGV GLAALGLIGV
160
MLSRNKKQKQ
Length:160
Mass (Da):17,749
Last modified:September 1, 2009 - v2
Checksum:iA0B32D6FA0A943A3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311A → T in CAA53991 (PubMed:8290259).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76453 mRNA. Translation: CAA53991.1.
AB298805 mRNA. Translation: BAH08634.1.
CH473953 Genomic DNA. Translation: EDM12684.1.
CH473953 Genomic DNA. Translation: EDM12685.1.
PIRiS42794.
RefSeqiNP_058756.2. NM_017060.2.
UniGeneiRn.11377.

Genome annotation databases

EnsembliENSRNOT00000032879; ENSRNOP00000038045; ENSRNOG00000021206.
GeneIDi24913.
KEGGirno:24913.
UCSCiRGD:2829. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76453 mRNA. Translation: CAA53991.1.
AB298805 mRNA. Translation: BAH08634.1.
CH473953 Genomic DNA. Translation: EDM12684.1.
CH473953 Genomic DNA. Translation: EDM12685.1.
PIRiS42794.
RefSeqiNP_058756.2. NM_017060.2.
UniGeneiRn.11377.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038045.

Proteomic databases

PaxDbiP53817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000032879; ENSRNOP00000038045; ENSRNOG00000021206.
GeneIDi24913.
KEGGirno:24913.
UCSCiRGD:2829. rat.

Organism-specific databases

CTDi11145.
RGDi2829. Pla2g16.

Phylogenomic databases

eggNOGiENOG410IW0Y. Eukaryota.
ENOG4111TDD. LUCA.
GeneTreeiENSGT00390000013327.
HOGENOMiHOG000293227.
InParanoidiP53817.
KOiK16817.
OMAiHVNNKHD.
OrthoDBiEOG7B8S59.
PhylomeDBiP53817.
TreeFamiTF330836.

Enzyme and pathway databases

BRENDAi3.1.1.32. 5301.
ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482801. Acyl chain remodelling of PS.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-1482922. Acyl chain remodelling of PI.

Miscellaneous databases

PROiP53817.

Gene expression databases

ExpressionAtlasiP53817. baseline and differential.
GenevisibleiP53817. RN.

Family and domain databases

InterProiIPR007053. LRAT-like_dom.
[Graphical view]
PfamiPF04970. LRAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Subtraction cloning of H-rev107, a gene specifically expressed in H-ras resistant fibroblasts."
    Hajnal A., Klemenz R., Schaefer R.
    Oncogene 9:479-490(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Purification and characterization of LRAT-like protein 3."
    Morishita J., Okamoto Y., Jin X.H., Tsuboi K., Ueda N.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  4. "Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA)."
    Duncan R.E., Sarkadi-Nagy E., Jaworski K., Ahmadian M., Sul H.S.
    J. Biol. Chem. 283:25428-25436(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiHRSL3_RAT
AccessioniPrimary (citable) accession number: P53817
Secondary accession number(s): B7X6T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 1, 2009
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.