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Protein

Smoothelin

Gene

SMTN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Structural protein of the cytoskeleton.

GO - Molecular functioni

  • actin binding Source: ProtInc
  • structural constituent of muscle Source: ProtInc

GO - Biological processi

  • muscle organ development Source: ProtInc
  • smooth muscle contraction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:G66-32265-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Smoothelin
Gene namesi
Name:SMTN
Synonyms:SMSMO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:11126. SMTN.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: HPA
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: ProtInc
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi6525.
OpenTargetsiENSG00000183963.
PharmGKBiPA35975.

Polymorphism and mutation databases

BioMutaiSMTN.
DMDMi338817991.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000719762 – 917SmoothelinAdd BLAST916

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei341PhosphoserineCombined sources1
Modified residuei351PhosphothreonineCombined sources1
Modified residuei357PhosphoserineCombined sources1
Modified residuei360PhosphothreonineCombined sources1
Modified residuei373PhosphothreonineCombined sources1
Modified residuei503PhosphoserineCombined sources1
Modified residuei514PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1
Modified residuei576PhosphoserineCombined sources1
Modified residuei729PhosphoserineCombined sources1
Modified residuei792PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP53814.
MaxQBiP53814.
PaxDbiP53814.
PeptideAtlasiP53814.
PRIDEiP53814.

PTM databases

iPTMnetiP53814.
PhosphoSitePlusiP53814.

Expressioni

Tissue specificityi

Smooth muscle; contractile or vascular (for the long form).

Gene expression databases

BgeeiENSG00000183963.
ExpressionAtlasiP53814. baseline and differential.
GenevisibleiP53814. HS.

Organism-specific databases

HPAiCAB001721.
HPA051778.
HPA058590.

Interactioni

GO - Molecular functioni

  • actin binding Source: ProtInc

Protein-protein interaction databases

BioGridi112416. 8 interactors.
IntActiP53814. 8 interactors.
STRINGi9606.ENSP00000351593.

Structurei

Secondary structure

1917
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi801 – 812Combined sources12
Beta strandi813 – 815Combined sources3
Turni824 – 829Combined sources6
Helixi832 – 841Combined sources10
Turni843 – 845Combined sources3
Helixi856 – 868Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D87NMR-A801-868[»]
ProteinModelPortaliP53814.
SMRiP53814.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53814.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini799 – 903CHPROSITE-ProRule annotationAdd BLAST105

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili24 – 89Sequence analysisAdd BLAST66
Coiled coili603 – 630Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi194 – 282Pro-richAdd BLAST89
Compositional biasi710 – 715Poly-Ser6

Sequence similaritiesi

Belongs to the smoothelin family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4678. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
HOVERGENiHBG076678.
InParanoidiP53814.
TreeFamiTF316716.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR022189. SMTN.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF12510. Smoothelin. 3 hits.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform B (identifier: P53814-1) [UniParc]FASTAAdd to basket
Also known as: Long, B1, L1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEALAGLD EGALRKLLEV TADLAERRRI RSAIRELQRQ ELEREEEALA
60 70 80 90 100
SKRFRAERQD NKENWLHSQQ REAEQRAALA RLAGQLESMN DVEELTALLR
110 120 130 140 150
SAGEYEERKL IRAAIRRVRA QEIEAATLAG RLYSGRPNSG SREDSKGLAA
160 170 180 190 200
HRLEQCEVPE REEQEQQAEV SKPTPTPEGT SQDVTTVTLL LRAPPGSTSS
210 220 230 240 250
SPASPSSSPT PASPEPPLEP AEAQCLTAEV PGSPEPPPSP PKTTSPEPQE
260 270 280 290 300
SPTLPSTEGQ VVNKLLSGPK ETPAAQSPTR GPSDTKRADV AGPRPCQRSL
310 320 330 340 350
SVLSPRQPAQ NRESTPLASG PSSFQRAGSV RDRVHKFTSD SPMAARLQDG
360 370 380 390 400
TPQAALSPLT PARLLGPSLT STTPASSSSG SSSRGPSDTS SRFSKEQRGV
410 420 430 440 450
AQPLAQLRSC PQEEGPRGRG LAARPLENRA GGPVARSEEP GAPLPVAVGT
460 470 480 490 500
AEPGGSMKTT FTIEIKDGRG QASTGRVLLP TGNQRAELTL GLRAPPTLLS
510 520 530 540 550
TSSGGKSTIT RVNSPGTLAR LGSVTHVTSF SHAPPSSRGG CSIKMEAEPA
560 570 580 590 600
EPLAAAVEAA NGAEQTRVNK APEGRSPLSA EELMTIEDEG VLDKMLDQST
610 620 630 640 650
DFEERKLIRA ALRELRQRKR DQRDKERERR LQEARGRPGE GRGNTATETT
660 670 680 690 700
TRHSQRAADG SAVSTVTKTE RLVHSNDGTR TARTTTVESS FVRRSENGSG
710 720 730 740 750
STMMQTKTFS SSSSSKKMGS IFDREDQASP RAGSLAALEK RQAEKKKELM
760 770 780 790 800
KAQSLPKTSA SQARKAMIEK LEKEGAAGSP GGPRAAVQRS TSFGVPNANS
810 820 830 840 850
IKQMLLDWCR AKTRGYEHVD IQNFSSSWSD GMAFCALVHN FFPEAFDYGQ
860 870 880 890 900
LSPQNRRQNF EVAFSSAEML VDCVPLVEVD DMMIMGKKPD PKCVFTYVQS
910
LYNHLRRHEL RLRGKNV
Length:917
Mass (Da):99,059
Last modified:June 28, 2011 - v7
Checksum:iEC508A8239AA6B36
GO
Isoform A (identifier: P53814-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-456: Missing.

Note: Produced by alternative promoter usage.
Show »
Length:461
Mass (Da):50,572
Checksum:iFE6EDC0D644E1B5D
GO
Isoform B2 (identifier: P53814-5) [UniParc]FASTAAdd to basket
Also known as: L2

The sequence of this isoform differs from the canonical sequence as follows:
     869-917: MLVDCVPLVE...HELRLRGKNV → THADCPQLLD...QKGLVKTKKS

Show »
Length:915
Mass (Da):98,919
Checksum:iFF0ABC36BBEC9B30
GO
Isoform B3 (identifier: P53814-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     868-868: E → ETHADCPQLLDTEDMVRLREPDWK

Show »
Length:940
Mass (Da):101,810
Checksum:iE9C48F69CEEE2847
GO

Sequence cautioni

The sequence AAL36149 differs from that shown. Reason: Frameshift at positions 362 and 384.Curated
The sequence AAL36150 differs from that shown. Reason: Frameshift at positions 362 and 384.Curated
The sequence CAA73884 differs from that shown. Reason: Frameshift at positions 362 and 384.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279T → P in AAF03562 (PubMed:12176134).Curated1
Sequence conflicti279T → P in AAL36149 (PubMed:12176134).Curated1
Sequence conflicti279T → P in AAL36150 (PubMed:12176134).Curated1
Sequence conflicti279T → P in CAA73884 (PubMed:12176134).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038785455G → D.4 PublicationsCorresponds to variant rs1064178dbSNPEnsembl.1
Natural variantiVAR_038786547A → P.4 PublicationsCorresponds to variant rs3205187dbSNPEnsembl.1
Natural variantiVAR_038787559A → V.Corresponds to variant rs5997872dbSNPEnsembl.1
Natural variantiVAR_038788580A → T.Corresponds to variant rs12158015dbSNPEnsembl.1
Natural variantiVAR_035657637R → Q in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs777851029dbSNPEnsembl.1
Natural variantiVAR_062223642R → C.Corresponds to variant rs34292278dbSNPEnsembl.1
Natural variantiVAR_035658763A → V in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0043981 – 456Missing in isoform A. 2 PublicationsAdd BLAST456
Alternative sequenceiVSP_031242868E → ETHADCPQLLDTEDMVRLRE PDWK in isoform B3. 1 Publication1
Alternative sequenceiVSP_007020869 – 917MLVDC…RGKNV → THADCPQLLDTEDMVRLREP DWKCVYTYIQEFYRCLVQKG LVKTKKS in isoform B2. 4 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49989 mRNA. Translation: CAA90281.2.
AJ010306 mRNA. Translation: CAA09077.2.
Y13492 mRNA. Translation: CAA73884.2. Frameshift.
AY061971 mRNA. Translation: AAL36149.1. Frameshift.
AY061972 mRNA. Translation: AAL36150.1. Frameshift.
AF115570
, AF115552, AF115553, AF115554, AF115555, AF115556, AF115557, AF115558, AF115559, AF115560, AF115561, AF115562, AF115563, AF115564, AF115565, AF115566, AF115567, AF115568, AF115569 Genomic DNA. Translation: AAF03562.1.
AF115570
, AF115560, AF115561, AF115562, AF115563, AF115564, AF115565, AF115566, AF115567, AF115568, AF115569 Genomic DNA. Translation: AAF03563.1.
AF064238 mRNA. Translation: AAF01481.3.
AC005005 Genomic DNA. Translation: AAD15619.1.
CH471095 Genomic DNA. Translation: EAW59930.1.
BC034237 mRNA. Translation: AAH34237.1.
CCDSiCCDS13886.1. [P53814-1]
CCDS13887.1. [P53814-6]
CCDS13888.1. [P53814-5]
PIRiT09575.
RefSeqiNP_001193946.1. NM_001207017.1.
NP_001193947.1. NM_001207018.1.
NP_008863.3. NM_006932.4. [P53814-1]
NP_599031.1. NM_134269.2. [P53814-5]
NP_599032.2. NM_134270.2. [P53814-6]
UniGeneiHs.149098.

Genome annotation databases

EnsembliENST00000333137; ENSP00000329532; ENSG00000183963. [P53814-5]
ENST00000347557; ENSP00000328635; ENSG00000183963. [P53814-1]
ENST00000358743; ENSP00000351593; ENSG00000183963. [P53814-6]
GeneIDi6525.
KEGGihsa:6525.
UCSCiuc003ajk.3. human. [P53814-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49989 mRNA. Translation: CAA90281.2.
AJ010306 mRNA. Translation: CAA09077.2.
Y13492 mRNA. Translation: CAA73884.2. Frameshift.
AY061971 mRNA. Translation: AAL36149.1. Frameshift.
AY061972 mRNA. Translation: AAL36150.1. Frameshift.
AF115570
, AF115552, AF115553, AF115554, AF115555, AF115556, AF115557, AF115558, AF115559, AF115560, AF115561, AF115562, AF115563, AF115564, AF115565, AF115566, AF115567, AF115568, AF115569 Genomic DNA. Translation: AAF03562.1.
AF115570
, AF115560, AF115561, AF115562, AF115563, AF115564, AF115565, AF115566, AF115567, AF115568, AF115569 Genomic DNA. Translation: AAF03563.1.
AF064238 mRNA. Translation: AAF01481.3.
AC005005 Genomic DNA. Translation: AAD15619.1.
CH471095 Genomic DNA. Translation: EAW59930.1.
BC034237 mRNA. Translation: AAH34237.1.
CCDSiCCDS13886.1. [P53814-1]
CCDS13887.1. [P53814-6]
CCDS13888.1. [P53814-5]
PIRiT09575.
RefSeqiNP_001193946.1. NM_001207017.1.
NP_001193947.1. NM_001207018.1.
NP_008863.3. NM_006932.4. [P53814-1]
NP_599031.1. NM_134269.2. [P53814-5]
NP_599032.2. NM_134270.2. [P53814-6]
UniGeneiHs.149098.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D87NMR-A801-868[»]
ProteinModelPortaliP53814.
SMRiP53814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112416. 8 interactors.
IntActiP53814. 8 interactors.
STRINGi9606.ENSP00000351593.

PTM databases

iPTMnetiP53814.
PhosphoSitePlusiP53814.

Polymorphism and mutation databases

BioMutaiSMTN.
DMDMi338817991.

Proteomic databases

EPDiP53814.
MaxQBiP53814.
PaxDbiP53814.
PeptideAtlasiP53814.
PRIDEiP53814.

Protocols and materials databases

DNASUi6525.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333137; ENSP00000329532; ENSG00000183963. [P53814-5]
ENST00000347557; ENSP00000328635; ENSG00000183963. [P53814-1]
ENST00000358743; ENSP00000351593; ENSG00000183963. [P53814-6]
GeneIDi6525.
KEGGihsa:6525.
UCSCiuc003ajk.3. human. [P53814-1]

Organism-specific databases

CTDi6525.
DisGeNETi6525.
GeneCardsiSMTN.
H-InvDBHIX0016383.
HGNCiHGNC:11126. SMTN.
HPAiCAB001721.
HPA051778.
HPA058590.
MIMi602127. gene.
neXtProtiNX_P53814.
OpenTargetsiENSG00000183963.
PharmGKBiPA35975.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4678. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
HOVERGENiHBG076678.
InParanoidiP53814.
TreeFamiTF316716.

Enzyme and pathway databases

BioCyciZFISH:G66-32265-MONOMER.

Miscellaneous databases

ChiTaRSiSMTN. human.
EvolutionaryTraceiP53814.
GeneWikiiSMTN.
GenomeRNAii6525.
PROiP53814.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183963.
ExpressionAtlasiP53814. baseline and differential.
GenevisibleiP53814. HS.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR022189. SMTN.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF12510. Smoothelin. 3 hits.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMTN_HUMAN
AccessioniPrimary (citable) accession number: P53814
Secondary accession number(s): O00569
, O95769, O95937, Q8N4H8, Q8WWW1, Q8WWW2, Q9P1S8, Q9UIT1, Q9UIT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 155 of the entry and version 7 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.